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The map label for this gene is miaB

Identifier: 66768109

GI number: 66768109

Start: 2174516

End: 2175973

Strand: Reverse

Name: miaB

Synonym: XC_1788

Alternate gene names: 66768109

Gene position: 2175973-2174516 (Counterclockwise)

Preceding gene: 66768110

Following gene: 66768108

Centisome position: 42.26

GC content: 64.2

Gene sequence:

>1458_bases
ATGCCCGGGACATCCGTTTCCGATCTGCCGACCACGGCCACCGCCGTGGACGCGCCCGCCCTGCTGCCCCTGCCCGTTGG
CCGCCCGCAAGCGCCTGCCCTGGTGCGCGGCAAGCTGTACATCAAGACCCACGGGTGCCAGATGAACGAGTACGACTCGG
CCAAGATGGCCGACGTGCTTGCCGCGTCCGAAGGACTGGAGCTCACCGACAATCCGGAAGACGCGGACGTGGTGCTGGTC
AACACCTGCTCCATTCGCGAAAAGGCGCAGGAAAAGGTGTTCAGCCAGTTGGGCCGCTGGAAGGCGCTCAAGGCCGGCGG
CAAGCCGGTGATCATCGGCGTGGGTGGCTGCGTGGCGTCGCAGGAAGGCGAAGCGATCGTCAAGCGCGCGCCGTACGTGG
ACCTGGTGTTTGGCCCGCAGACCCTGCACCGGCTGCCGGAGTTGATCCGTGCGCGCCGTGAGTCCGGCAAGTCGCAGGTG
GACATCAGCTTCCCGGAGATCGAGAAGTTCGACCGCCTGCCCGAACCGCGTGCCGATGGCCCGTCGGCGTTCGTGTCGAT
CATGGAAGGTTGTTCCAAGTACTGCTCGTTCTGCGTGGTGCCATATACACGTGGCGAAGAAGTCAGCCGGCCGTTCGAGG
ACGTGCTGGTGGAAGTGGCGCAGCTGGCTGCACAAGGCGTGCGCGAGATCAATCTGCTCGGCCAGAACGTCAACGCGTAT
CGCGGTGCGTACGGTGCCGATGCTGGCGAGCCGGCGCAGTACGCCGATCTGGGGTTGCTGATCCGTACCATTGCGCAGAT
CGACGGGATTGGCCGGATCCGCTTTACCACCTCGCACCCGCTGGAGTTTTCCGATTCGCTGGTCGATGCCTACCGCGATG
TGCCGCAGCTGGCCAATTACCTGCATCTGCCGGTGCAGGCCGGCAGCGACCGCATCCTGTCGGCGATGAAGCGCGGCTAC
ACCGCACTGGAATTCAAGTCCAAGATCCGCAAGCTGCGCGCAGTGCGGCCAGACATCTCGATCAGCTCCGACTTCATCGT
CGGCTTTCCGGGCGAGACCGACGCCGATTTCGACAAGACCATGAAGTTGATCGAGGACGTGGGCTTCGACCAGAGCTTCT
CCTTTATCTACTCGCGTCGCCCCGGTACGCCGGCATCGGATCTGGAAGACGACACCCCCGATGCGGTGAAGCAGGCTCGC
CTTGCACGTCTGCAGGCGCATATCAACGCGCATGCCGCCGGCATTTCGCAACGCATGGTCGGCAGCGTGCAACGCGTGCT
GGTGGAAGGCCCGTCGCGCCGTGATGCCAATGAGCTCACCGGCAAGACCGAAAACATGCGCCCGGTGAATTTCCCGGGGA
ATCCGCGCCTGGTGGGCCAGTTCGTGGATGTGCTGATCACCGAAGCACTGAGCAATTCGCTGCGCGGGCGCATCCAATTG
GACGACAGCGCGGCCTGA

Upstream 100 bases:

>100_bases
GGCGCGGGCCGCGGCGGTCGATGCCGGCGCCTGACCGCCACCGGGGCGGCGTGAGCGGGTAAACTGCGCGGCTATCTTCT
ACCCGCCTGGAATAAGCGCC

Downstream 100 bases:

>100_bases
GCGGGCAGCGCGCATTCTTGCCCTCCGGGAGACCTCACACGCTGATGCAGTTGGCGTGTGGTGGTCGCCCAGGTGCAGGC
CGACGACGGTGGCGAGACAT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MPGTSVSDLPTTATAVDAPALLPLPVGRPQAPALVRGKLYIKTHGCQMNEYDSAKMADVLAASEGLELTDNPEDADVVLV
NTCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQV
DISFPEIEKFDRLPEPRADGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAY
RGAYGADAGEPAQYADLGLLIRTIAQIDGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYLHLPVQAGSDRILSAMKRGY
TALEFKSKIRKLRAVRPDISISSDFIVGFPGETDADFDKTMKLIEDVGFDQSFSFIYSRRPGTPASDLEDDTPDAVKQAR
LARLQAHINAHAAGISQRMVGSVQRVLVEGPSRRDANELTGKTENMRPVNFPGNPRLVGQFVDVLITEALSNSLRGRIQL
DDSAA

Sequences:

>Translated_485_residues
MPGTSVSDLPTTATAVDAPALLPLPVGRPQAPALVRGKLYIKTHGCQMNEYDSAKMADVLAASEGLELTDNPEDADVVLV
NTCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQV
DISFPEIEKFDRLPEPRADGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAY
RGAYGADAGEPAQYADLGLLIRTIAQIDGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYLHLPVQAGSDRILSAMKRGY
TALEFKSKIRKLRAVRPDISISSDFIVGFPGETDADFDKTMKLIEDVGFDQSFSFIYSRRPGTPASDLEDDTPDAVKQAR
LARLQAHINAHAAGISQRMVGSVQRVLVEGPSRRDANELTGKTENMRPVNFPGNPRLVGQFVDVLITEALSNSLRGRIQL
DDSAA
>Mature_484_residues
PGTSVSDLPTTATAVDAPALLPLPVGRPQAPALVRGKLYIKTHGCQMNEYDSAKMADVLAASEGLELTDNPEDADVVLVN
TCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVD
ISFPEIEKFDRLPEPRADGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAYR
GAYGADAGEPAQYADLGLLIRTIAQIDGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYLHLPVQAGSDRILSAMKRGYT
ALEFKSKIRKLRAVRPDISISSDFIVGFPGETDADFDKTMKLIEDVGFDQSFSFIYSRRPGTPASDLEDDTPDAVKQARL
ARLQAHINAHAAGISQRMVGSVQRVLVEGPSRRDANELTGKTENMRPVNFPGNPRLVGQFVDVLITEALSNSLRGRIQLD
DSAA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=487, Percent_Identity=38.1930184804928, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI28872782, Length=487, Percent_Identity=38.1930184804928, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI93277076, Length=451, Percent_Identity=29.490022172949, Blast_Score=176, Evalue=4e-44,
Organism=Escherichia coli, GI1786882, Length=442, Percent_Identity=66.9683257918552, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1787057, Length=423, Percent_Identity=28.8416075650118, Blast_Score=161, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17553146, Length=461, Percent_Identity=35.3579175704989, Blast_Score=257, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI71996771, Length=311, Percent_Identity=30.5466237942122, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21356207, Length=474, Percent_Identity=33.3333333333333, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=30.0653594771242, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_XANC8 (Q4UVS2)

Other databases:

- EMBL:   CP000050
- RefSeq:   YP_242871.1
- ProteinModelPortal:   Q4UVS2
- SMR:   Q4UVS2
- STRING:   Q4UVS2
- GeneID:   3380335
- GenomeReviews:   CP000050_GR
- KEGG:   xcb:XC_1788
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q4UVS2
- ProtClustDB:   PRK14325
- BioCyc:   XCAM314565:XC_1788-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52738; Mature: 52607

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTSVSDLPTTATAVDAPALLPLPVGRPQAPALVRGKLYIKTHGCQMNEYDSAKMADVL
CCCCCCCCCCCCHHHCCCCCEEECCCCCCCCCCEEECEEEEEECCCCCCCCCCHHHHHHH
AASEGLELTDNPEDADVVLVNTCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVAS
HHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCEECC
QEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVDISFPEIEKFDRLPEPRADG
CCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCC
PSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAY
HHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RGAYGADAGEPAQYADLGLLIRTIAQIDGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANY
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LHLPVQAGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSDFIVGFPGETDADFDKT
EECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHH
MKLIEDVGFDQSFSFIYSRRPGTPASDLEDDTPDAVKQARLARLQAHINAHAAGISQRMV
HHHHHHCCCCCCCHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSVQRVLVEGPSRRDANELTGKTENMRPVNFPGNPRLVGQFVDVLITEALSNSLRGRIQL
HHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
DDSAA
CCCCC
>Mature Secondary Structure 
PGTSVSDLPTTATAVDAPALLPLPVGRPQAPALVRGKLYIKTHGCQMNEYDSAKMADVL
CCCCCCCCCCCHHHCCCCCEEECCCCCCCCCCEEECEEEEEECCCCCCCCCCHHHHHHH
AASEGLELTDNPEDADVVLVNTCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVAS
HHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCEECC
QEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVDISFPEIEKFDRLPEPRADG
CCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCC
PSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAY
HHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RGAYGADAGEPAQYADLGLLIRTIAQIDGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANY
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LHLPVQAGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSDFIVGFPGETDADFDKT
EECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHH
MKLIEDVGFDQSFSFIYSRRPGTPASDLEDDTPDAVKQARLARLQAHINAHAAGISQRMV
HHHHHHCCCCCCCHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSVQRVLVEGPSRRDANELTGKTENMRPVNFPGNPRLVGQFVDVLITEALSNSLRGRIQL
HHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
DDSAA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA