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The map label for this gene is potI [H]

Identifier: 66768099

GI number: 66768099

Start: 2163264

End: 2164112

Strand: Direct

Name: potI [H]

Synonym: XC_1778

Alternate gene names: 66768099

Gene position: 2163264-2164112 (Clockwise)

Preceding gene: 77761166

Following gene: 66768101

Centisome position: 42.02

GC content: 64.66

Gene sequence:

>849_bases
ATGATGCGCGCCGCGCGCGGTGGGCGTGTGCTGGGCGGCAGCGTGCTCGCGCTGGGGTTCGGTTTTTTGTATCTGCCGAT
CCTGTTGCTGATGGTGTATTCGTTCAACGCCTCGCGCCTGGCCACGGTGTGGGGCGGATTTTCCACCCGCTGGTATGGCG
AGTTGCTGCGTGACCGCCAATTGCTGGACGCGGCCTGGGTGAGCCTGGAAGTGGCGTTCTGGACCGCATGCGCGTCCACC
GTGCTCGGCACCATGGCGGCGATGGCGATGGTGCGCATGCGGCGGTTTCCCGGCAAAACGCTGTTCGGTGCGCTGATCAC
CGCACCGCTGGTGATGCCGGAGGTGATCATTGGTCTGTCGATCCTGTTGCTGCTGGTCTCGATGGGCGGCGTGCTCGGCA
TCGCGCCGCGCGGCGCGGTCGCCATCTGGGTGGCGCATGTCACCTTCACCGTCTCATTCGTGACCGTGGTGATTTCCTCG
CGGCTGCAGGAGCTGGACCGCTCGCTGGAAGAAGCCGCCATGGATCTGGGCGCCACGCCACTGAAGGTGTTCTTCCTGAT
CACCTTGCCGATCATCGCGCCAGCACTTGCGTCGGGCTGGCTGTTGGCATTCACGCTCTCGCTGGACGATGTGGTCATTG
CCAGTTTCCTGGCGGGCCCAAGCTCCACCACGCTGCCGATCAAGGTGTTTTCGTCGGTACGCCAGGGCATCAGCCCCAAG
ATCAATGCGCTGGCCACGGTGATGGTGCTGGCGGTGTCGGTGGCGGCGGTGATCGGGTGGTGGTTGCTGGCGCGCAGTGA
AAAACGCCGCCTGCGTGATGCGCAACTTGCGCTGCAGTCTGCTCCCTGA

Upstream 100 bases:

>100_bases
CGGTGGCCGCCGCGGTAGCCACGGTGATGCTGGTGCTGCTGCTGGTGCCGATCGTGATCTTCCACCGTTACCAGCAGCGC
GAACTGGAAGGGCGGCTGAC

Downstream 100 bases:

>100_bases
GGGCGCGCCGGCGCTGAGGTGGCCAATGATCGCAGCGACCTTGCCGCCAGATAGATGCAAACGCTAGAGCCACCGGTGTG
TGCGAATGCTGCAAGTCGAT

Product: putrescine transport protein; permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MMRAARGGRVLGGSVLALGFGFLYLPILLLMVYSFNASRLATVWGGFSTRWYGELLRDRQLLDAAWVSLEVAFWTACAST
VLGTMAAMAMVRMRRFPGKTLFGALITAPLVMPEVIIGLSILLLLVSMGGVLGIAPRGAVAIWVAHVTFTVSFVTVVISS
RLQELDRSLEEAAMDLGATPLKVFFLITLPIIAPALASGWLLAFTLSLDDVVIASFLAGPSSTTLPIKVFSSVRQGISPK
INALATVMVLAVSVAAVIGWWLLARSEKRRLRDAQLALQSAP

Sequences:

>Translated_282_residues
MMRAARGGRVLGGSVLALGFGFLYLPILLLMVYSFNASRLATVWGGFSTRWYGELLRDRQLLDAAWVSLEVAFWTACAST
VLGTMAAMAMVRMRRFPGKTLFGALITAPLVMPEVIIGLSILLLLVSMGGVLGIAPRGAVAIWVAHVTFTVSFVTVVISS
RLQELDRSLEEAAMDLGATPLKVFFLITLPIIAPALASGWLLAFTLSLDDVVIASFLAGPSSTTLPIKVFSSVRQGISPK
INALATVMVLAVSVAAVIGWWLLARSEKRRLRDAQLALQSAP
>Mature_282_residues
MMRAARGGRVLGGSVLALGFGFLYLPILLLMVYSFNASRLATVWGGFSTRWYGELLRDRQLLDAAWVSLEVAFWTACAST
VLGTMAAMAMVRMRRFPGKTLFGALITAPLVMPEVIIGLSILLLLVSMGGVLGIAPRGAVAIWVAHVTFTVSFVTVVISS
RLQELDRSLEEAAMDLGATPLKVFFLITLPIIAPALASGWLLAFTLSLDDVVIASFLAGPSSTTLPIKVFSSVRQGISPK
INALATVMVLAVSVAAVIGWWLLARSEKRRLRDAQLALQSAP

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=264, Percent_Identity=62.1212121212121, Blast_Score=300, Evalue=8e-83,
Organism=Escherichia coli, GI1787368, Length=262, Percent_Identity=40.0763358778626, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1787714, Length=253, Percent_Identity=32.8063241106719, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788764, Length=208, Percent_Identity=31.25, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30221; Mature: 30221

Theoretical pI: Translated: 11.16; Mature: 11.16

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRAARGGRVLGGSVLALGFGFLYLPILLLMVYSFNASRLATVWGGFSTRWYGELLRDRQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHH
LLDAAWVSLEVAFWTACASTVLGTMAAMAMVRMRRFPGKTLFGALITAPLVMPEVIIGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ILLLLVSMGGVLGIAPRGAVAIWVAHVTFTVSFVTVVISSRLQELDRSLEEAAMDLGATP
HHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LKVFFLITLPIIAPALASGWLLAFTLSLDDVVIASFLAGPSSTTLPIKVFSSVRQGISPK
HHHHHHHHHHHHHHHHHCCHHHHEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCH
INALATVMVLAVSVAAVIGWWLLARSEKRRLRDAQLALQSAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMRAARGGRVLGGSVLALGFGFLYLPILLLMVYSFNASRLATVWGGFSTRWYGELLRDRQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHH
LLDAAWVSLEVAFWTACASTVLGTMAAMAMVRMRRFPGKTLFGALITAPLVMPEVIIGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ILLLLVSMGGVLGIAPRGAVAIWVAHVTFTVSFVTVVISSRLQELDRSLEEAAMDLGATP
HHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LKVFFLITLPIIAPALASGWLLAFTLSLDDVVIASFLAGPSSTTLPIKVFSSVRQGISPK
HHHHHHHHHHHHHHHHHCCHHHHEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCH
INALATVMVLAVSVAAVIGWWLLARSEKRRLRDAQLALQSAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]