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The map label for this gene is mexA [H]

Identifier: 66768070

GI number: 66768070

Start: 2117763

End: 2119073

Strand: Reverse

Name: mexA [H]

Synonym: XC_1748

Alternate gene names: 66768070

Gene position: 2119073-2117763 (Counterclockwise)

Preceding gene: 66768071

Following gene: 66768069

Centisome position: 41.16

GC content: 70.18

Gene sequence:

>1311_bases
ATGATTGCCCCGCTCCGCACCTTCGGCCTGGCCTGTGCCATCACCGTGGCGCTCGCTGCCTGCAGCAAGCCCGAACAACA
GCAGGCTCCGCCGCCGCCGGAAGTGTCGGTGCTGGAAATGAAACCGCAGACGCTGCCGCTGGAACGTGACCTGGTTGGTC
GCCTCTCGGCGTTCCGCTCGGCCGACGTGCGCGCCCGTGTGGATGGTGTGCTGCTCAAGCGCCTGTACACCGAAGGCACC
GACGTCAAGGAAGGCCAGCCGCTGTTCGAGATCGACCCGATGCCGCTGCGCGCAACGCTGCTGCAGGCACAGGGCCAGCT
GGCTGCCGCCGAGGCCACCTATGCCAACGCCAAGATCGCCGCGCAGCGCGCGCGCAGCCTGGCGCCGCAGCAGTACGTCT
CGCGCGCCGACATCGACACCGCCGAAGCCACCGAGCGCTCGTCCGGCGCCAGCGTGCAGCAGGCGCGCGGTGTGGTCGAG
TCCGCAAGCATCCAGCTCAGCTTCGCCAGCGTGACCTCGCCGATCACCGGCCGCGCCGGCATTCAGCGCGTCACCGAAGG
CGCGCTGGTCGGGTCGGGTGAAGCCACCTTGCTGACCACGGTCGACCAGATCGACCCGCTGTACGTGAACTTCGCCATGA
GCAGCGAAGAACTGGCGGCACTGCGTCAGGCGCAGAGCAGTGGCAATGTGCAGCTCAGCGGCGACGGCAAGTCCACCATC
AACGTGGAGCTGGGCAACGGCACGCAGTACCCGCACCCCGGCACGCTGGACGTGTCCGCGGTGACGGTGGACCCGAGCAC
CGGTGCGGTGTCGCTGCGCGCCACCCTGCCCAACCCGGAAATGGCGCTGCTGCCAGGCGCGTTCGTAACCTTCAAGGCCA
GCCTGGGCCAGCGCAACAACGCCTATCTCGTGCCGCAGCAGGCGCTGCAGCGCGATGCCACCGGCGCCTACGCGCTGGTG
CTGGGCAAGGACGGCAAGGTGGTCCGCAAGAACCTCACCGTCGACGGCCAGCAGAAAGGCCAGTGGATCGTCACCGCCGG
CATGGCACCGGGCGACCAGGTCATCGTCGACGGCGTGCAGAAGGCGAAGGAAGGCCAGCCCGCCAAGGGCGTGCCGTGGG
ATCCGAACAAGCCCGCACAAGGCGGCCAGCCCGGTGCGCCGGGTGCCGCCCCGGCCGGGGGTCAGGCCGGCACCGGCCAG
GGCGCACCGGCCGGTGCCGGCAAGGGCGACGCCGGCGGTCCGGCGGGTGGCGAACAGCCCAAGTCCGATGACGCGGGCCA
GCCGTCCGATTCCCAGTCCAAGCAGCAGTGA

Upstream 100 bases:

>100_bases
CTGTCGACACGTTTATGCGTGCGTTCGCCGCGGTGGTCACGCCGCCCCGGACGCCCGCGCGTTCCTTTCTCACTTCAAGA
CCCTAACGGAGTTCCTCCTC

Downstream 100 bases:

>100_bases
CGGAACCCCGCCATGCCTAAGTTTTTTATCGAACACCCGGTCTTTGCCTGGGTGGTGGCGATCCTGATCTCGCTCGCGGG
CGTGATCTCGATCCTGAATC

Product: multidrug resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MIAPLRTFGLACAITVALAACSKPEQQQAPPPPEVSVLEMKPQTLPLERDLVGRLSAFRSADVRARVDGVLLKRLYTEGT
DVKEGQPLFEIDPMPLRATLLQAQGQLAAAEATYANAKIAAQRARSLAPQQYVSRADIDTAEATERSSGASVQQARGVVE
SASIQLSFASVTSPITGRAGIQRVTEGALVGSGEATLLTTVDQIDPLYVNFAMSSEELAALRQAQSSGNVQLSGDGKSTI
NVELGNGTQYPHPGTLDVSAVTVDPSTGAVSLRATLPNPEMALLPGAFVTFKASLGQRNNAYLVPQQALQRDATGAYALV
LGKDGKVVRKNLTVDGQQKGQWIVTAGMAPGDQVIVDGVQKAKEGQPAKGVPWDPNKPAQGGQPGAPGAAPAGGQAGTGQ
GAPAGAGKGDAGGPAGGEQPKSDDAGQPSDSQSKQQ

Sequences:

>Translated_436_residues
MIAPLRTFGLACAITVALAACSKPEQQQAPPPPEVSVLEMKPQTLPLERDLVGRLSAFRSADVRARVDGVLLKRLYTEGT
DVKEGQPLFEIDPMPLRATLLQAQGQLAAAEATYANAKIAAQRARSLAPQQYVSRADIDTAEATERSSGASVQQARGVVE
SASIQLSFASVTSPITGRAGIQRVTEGALVGSGEATLLTTVDQIDPLYVNFAMSSEELAALRQAQSSGNVQLSGDGKSTI
NVELGNGTQYPHPGTLDVSAVTVDPSTGAVSLRATLPNPEMALLPGAFVTFKASLGQRNNAYLVPQQALQRDATGAYALV
LGKDGKVVRKNLTVDGQQKGQWIVTAGMAPGDQVIVDGVQKAKEGQPAKGVPWDPNKPAQGGQPGAPGAAPAGGQAGTGQ
GAPAGAGKGDAGGPAGGEQPKSDDAGQPSDSQSKQQ
>Mature_436_residues
MIAPLRTFGLACAITVALAACSKPEQQQAPPPPEVSVLEMKPQTLPLERDLVGRLSAFRSADVRARVDGVLLKRLYTEGT
DVKEGQPLFEIDPMPLRATLLQAQGQLAAAEATYANAKIAAQRARSLAPQQYVSRADIDTAEATERSSGASVQQARGVVE
SASIQLSFASVTSPITGRAGIQRVTEGALVGSGEATLLTTVDQIDPLYVNFAMSSEELAALRQAQSSGNVQLSGDGKSTI
NVELGNGTQYPHPGTLDVSAVTVDPSTGAVSLRATLPNPEMALLPGAFVTFKASLGQRNNAYLVPQQALQRDATGAYALV
LGKDGKVVRKNLTVDGQQKGQWIVTAGMAPGDQVIVDGVQKAKEGQPAKGVPWDPNKPAQGGQPGAPGAAPAGGQAGTGQ
GAPAGAGKGDAGGPAGGEQPKSDDAGQPSDSQSKQQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=356, Percent_Identity=39.8876404494382, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789665, Length=380, Percent_Identity=39.2105263157895, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1786668, Length=380, Percent_Identity=40, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI87082036, Length=315, Percent_Identity=33.3333333333333, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44796; Mature: 44796

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAPLRTFGLACAITVALAACSKPEQQQAPPPPEVSVLEMKPQTLPLERDLVGRLSAFRS
CCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHC
ADVRARVDGVLLKRLYTEGTDVKEGQPLFEIDPMPLRATLLQAQGQLAAAEATYANAKIA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHCCCEEEHHHHHHHHHHH
AQRARSLAPQQYVSRADIDTAEATERSSGASVQQARGVVESASIQLSFASVTSPITGRAG
HHHHHHCCHHHHHHHCCCCHHHHHCCCCCCCHHHHHCCHHHCEEEEEEHHHCCCCCCHHH
IQRVTEGALVGSGEATLLTTVDQIDPLYVNFAMSSEELAALRQAQSSGNVQLSGDGKSTI
HHHHHCCCEECCCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEECCCCEEE
NVELGNGTQYPHPGTLDVSAVTVDPSTGAVSLRATLPNPEMALLPGAFVTFKASLGQRNN
EEEECCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCEEEECCCEEEEEECCCCCCC
AYLVPQQALQRDATGAYALVLGKDGKVVRKNLTVDGQQKGQWIVTAGMAPGDQVIVDGVQ
EEECCHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHH
KAKEGQPAKGVPWDPNKPAQGGQPGAPGAAPAGGQAGTGQGAPAGAGKGDAGGPAGGEQP
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KSDDAGQPSDSQSKQQ
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIAPLRTFGLACAITVALAACSKPEQQQAPPPPEVSVLEMKPQTLPLERDLVGRLSAFRS
CCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHC
ADVRARVDGVLLKRLYTEGTDVKEGQPLFEIDPMPLRATLLQAQGQLAAAEATYANAKIA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHCCCEEEHHHHHHHHHHH
AQRARSLAPQQYVSRADIDTAEATERSSGASVQQARGVVESASIQLSFASVTSPITGRAG
HHHHHHCCHHHHHHHCCCCHHHHHCCCCCCCHHHHHCCHHHCEEEEEEHHHCCCCCCHHH
IQRVTEGALVGSGEATLLTTVDQIDPLYVNFAMSSEELAALRQAQSSGNVQLSGDGKSTI
HHHHHCCCEECCCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEECCCCEEE
NVELGNGTQYPHPGTLDVSAVTVDPSTGAVSLRATLPNPEMALLPGAFVTFKASLGQRNN
EEEECCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCEEEECCCEEEEEECCCCCCC
AYLVPQQALQRDATGAYALVLGKDGKVVRKNLTVDGQQKGQWIVTAGMAPGDQVIVDGVQ
EEECCHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHH
KAKEGQPAKGVPWDPNKPAQGGQPGAPGAAPAGGQAGTGQGAPAGAGKGDAGGPAGGEQP
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KSDDAGQPSDSQSKQQ
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]