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The map label for this gene is queA [H]

Identifier: 66768058

GI number: 66768058

Start: 2097956

End: 2099026

Strand: Direct

Name: queA [H]

Synonym: XC_1735

Alternate gene names: 66768058

Gene position: 2097956-2099026 (Clockwise)

Preceding gene: 66768057

Following gene: 66768059

Centisome position: 40.75

GC content: 68.72

Gene sequence:

>1071_bases
TTGAAGAAGTCCGATTTCCATTACGACCTGCCCGAAGAACTGATCGCCCAGGCGCCGTTGGCCGAGCGGGCGGCCAGCCG
TTTGCTGGTGGTGCCGCCGACGCCGGCCGCCTTCAGTGATCGACAGGTGCGCGATCTGCCTGAGCTGCTGCAGCCGGGCG
ACCTGCTGATCTTCAACGACACCCGGGTGATCCCGGCGCGGTTGTTCGGGCAGAAGGCCAGCGGCGGGCGGGTGGAGATC
CTGATCGAGCGGCTGCTCGGCGGGCAGCAGGCGCGGGCGCAGATCGGCGCCAGCAAGTCGCCCAAGGCCGGCAGCGTGAT
CGCGCTGGACGCCGGTGGCCAGGCCGAAGTGCTGGGCCGCGACGGCGAGTTCTACCTGCTGCGTTTCGACATCCCCGCGC
CGCTGGAGCATTGGTTGCTCGACGCCGGGCGCCTGCCGCTGCCGCCGTACATCCGCCGCGAGCCGGGGCTGGACGATCGC
GAGCGCTACCAGACCGTGTTCGCGCGCGAAGTGGGCGCGGTCGCTGCGCCGACCGCCGGCCTGCATTTCGATGAAGCCCT
GCTGGCGCGCCTGCGCGAGCGCGGGGTGGAGTTCGGCCATGTGACCTTGCACGTGGGCGCCGGCACCTTCCAGCCGGTGC
GCGTGGATGCGCTGGACAAGCATGTGATGCACACCGAGTGGCTCAATGTGGGCGCCGCGCTGGTCGAGCAGGTGCGCCGC
ACCCGTGCGCGCGGTGGCCGCGTGATCGCGGTGGGCACCACCGTGGTGCGCTCGCTGGAAAGCGCCTGGCGCAAGACCGA
CGACGCCCCGCACGGCGAATTGCAGTCGTTCGCCGGCGAAACCCAGATCTTCATCCTGCCCGGCTACCGCATCCGCAGCG
TCGATGCGATGGTCACCAATTTCCACCTGCCCGAAAGCACGCTGCTGATGATGGTCTCGGCCTTCGCCGGCTGCGACCGC
ATCTTCGCCGCCTACGCACACGCCATCGCGCAGCGCTATCGCTTCTTCTCCTATGGCGATGCGATGTTGTTGTGGAGCAG
GGAGTGGGGAATCGGGAATGGGGAATCGTAG

Upstream 100 bases:

>100_bases
CGATCTCCGCAGGTCTTGTTTCCCTGGAGGAAAAGGCTGCCAAACCTGCCTCAGCATGTCCCGCTAAACTAGCGCCCCCA
AACCGCTGTACCGCCACGCC

Downstream 100 bases:

>100_bases
GGCTTGCCGCCACACGTTCCGCCATCGCCACCCGCTTCTCCGATTCCCGACTCCCGATTCCCCATTCCCCATTCCCGGCC
TTCCAATGTCCCGACTCCAG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKKSDFHYDLPEELIAQAPLAERAASRLLVVPPTPAAFSDRQVRDLPELLQPGDLLIFNDTRVIPARLFGQKASGGRVEI
LIERLLGGQQARAQIGASKSPKAGSVIALDAGGQAEVLGRDGEFYLLRFDIPAPLEHWLLDAGRLPLPPYIRREPGLDDR
ERYQTVFAREVGAVAAPTAGLHFDEALLARLRERGVEFGHVTLHVGAGTFQPVRVDALDKHVMHTEWLNVGAALVEQVRR
TRARGGRVIAVGTTVVRSLESAWRKTDDAPHGELQSFAGETQIFILPGYRIRSVDAMVTNFHLPESTLLMMVSAFAGCDR
IFAAYAHAIAQRYRFFSYGDAMLLWSREWGIGNGES

Sequences:

>Translated_356_residues
MKKSDFHYDLPEELIAQAPLAERAASRLLVVPPTPAAFSDRQVRDLPELLQPGDLLIFNDTRVIPARLFGQKASGGRVEI
LIERLLGGQQARAQIGASKSPKAGSVIALDAGGQAEVLGRDGEFYLLRFDIPAPLEHWLLDAGRLPLPPYIRREPGLDDR
ERYQTVFAREVGAVAAPTAGLHFDEALLARLRERGVEFGHVTLHVGAGTFQPVRVDALDKHVMHTEWLNVGAALVEQVRR
TRARGGRVIAVGTTVVRSLESAWRKTDDAPHGELQSFAGETQIFILPGYRIRSVDAMVTNFHLPESTLLMMVSAFAGCDR
IFAAYAHAIAQRYRFFSYGDAMLLWSREWGIGNGES
>Mature_356_residues
MKKSDFHYDLPEELIAQAPLAERAASRLLVVPPTPAAFSDRQVRDLPELLQPGDLLIFNDTRVIPARLFGQKASGGRVEI
LIERLLGGQQARAQIGASKSPKAGSVIALDAGGQAEVLGRDGEFYLLRFDIPAPLEHWLLDAGRLPLPPYIRREPGLDDR
ERYQTVFAREVGAVAAPTAGLHFDEALLARLRERGVEFGHVTLHVGAGTFQPVRVDALDKHVMHTEWLNVGAALVEQVRR
TRARGGRVIAVGTTVVRSLESAWRKTDDAPHGELQSFAGETQIFILPGYRIRSVDAMVTNFHLPESTLLMMVSAFAGCDR
IFAAYAHAIAQRYRFFSYGDAMLLWSREWGIGNGES

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=354, Percent_Identity=51.4124293785311, Blast_Score=352, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39295; Mature: 39295

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSDFHYDLPEELIAQAPLAERAASRLLVVPPTPAAFSDRQVRDLPELLQPGDLLIFND
CCCCCCCCCCCHHHHHCCCHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECC
TRVIPARLFGQKASGGRVEILIERLLGGQQARAQIGASKSPKAGSVIALDAGGQAEVLGR
CCEEHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEC
DGEFYLLRFDIPAPLEHWLLDAGRLPLPPYIRREPGLDDRERYQTVFAREVGAVAAPTAG
CCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
LHFDEALLARLRERGVEFGHVTLHVGAGTFQPVRVDALDKHVMHTEWLNVGAALVEQVRR
CCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TRARGGRVIAVGTTVVRSLESAWRKTDDAPHGELQSFAGETQIFILPGYRIRSVDAMVTN
HHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEEECCCEEEHHHHHHHC
FHLPESTLLMMVSAFAGCDRIFAAYAHAIAQRYRFFSYGDAMLLWSREWGIGNGES
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKKSDFHYDLPEELIAQAPLAERAASRLLVVPPTPAAFSDRQVRDLPELLQPGDLLIFND
CCCCCCCCCCCHHHHHCCCHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECC
TRVIPARLFGQKASGGRVEILIERLLGGQQARAQIGASKSPKAGSVIALDAGGQAEVLGR
CCEEHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEC
DGEFYLLRFDIPAPLEHWLLDAGRLPLPPYIRREPGLDDRERYQTVFAREVGAVAAPTAG
CCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
LHFDEALLARLRERGVEFGHVTLHVGAGTFQPVRVDALDKHVMHTEWLNVGAALVEQVRR
CCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TRARGGRVIAVGTTVVRSLESAWRKTDDAPHGELQSFAGETQIFILPGYRIRSVDAMVTN
HHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEEECCCEEEHHHHHHHC
FHLPESTLLMMVSAFAGCDRIFAAYAHAIAQRYRFFSYGDAMLLWSREWGIGNGES
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]