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The map label for this gene is wcaJ [H]

Identifier: 66767984

GI number: 66767984

Start: 1996080

End: 1997534

Strand: Direct

Name: wcaJ [H]

Synonym: XC_1660

Alternate gene names: 66767984

Gene position: 1996080-1997534 (Clockwise)

Preceding gene: 66767983

Following gene: 66767985

Centisome position: 38.77

GC content: 61.03

Gene sequence:

>1455_bases
ATGCTTTTGGCAGACTTGAGTAGCGCGACTTACACGACATCCTCGCCGCGATTGTTGTCCAAATATTCGGCAGCCGCCGA
CCTGGTCTTGCGAGTGTTCGACCTGACCATGGTCGTGGCGTCCGGACTGATCGCATACCGCATCGTTTTCGGTACCTGGG
TACCCGCAGCGCCTTATCGTGTCGCGATTGCAACAACGTTGTTGTATTCGGTGATCTGCTTCGCGTTATTCCCGCTGTAT
CGCAGCTGGCGCGGCCGTGGGTTGCTGAGTGAGCTGATGGTGCTGGGCGGTGCGTTCGGCGGCGTGTTTGCGCTGTTCGC
GGTGCATGCATTGATCGTGCAGGTGGGTGAGCAGGTGTCGCGTGGCTGGATTGGCCTGTGGTTCGTCGGCGGCCTGGTGT
CGCTGGTGGCCGCACGCACCTTGCTGCGTGGCTTCCTCAATCATCTGCGTACGCAGGGTGTGGATGTCCAGCGCGTGGTG
GTGGTCGGTCTGCGTCATCCAGTGATGAAGATCAGTCATTACTTGAGCCGTAACCCGTGGGTCGGTATGAACCTGGTGGG
CTACTTCCGCACGCCGTACGACCTGGCAGTGACCGATCAGCGCCAGGGCCTGCCGTGCCTGGGTGATCCTGATGAGTTGA
TCGAGTACCTGAAGAACAACCAGGTGGAGCAGGTGTGGATCTCGTTGCCGCTTGGCGAGCGCGACCACATCAAGCAGCTG
CTGCAGCGTCTGGATCGCTACCCGATCAACGTGAAGCTGGTGCCCGACCTGTTTGACTTCGGCCTGTTGAACCAGTCCGC
CGAGCAGATCGGCAGCGTGCCGGTGATCAACCTGCGCCAGGGTGGCGTGGATCGTGATAACTACTTCGTGGTCGCCAAGG
CGCTGCAGGACAAGATCCTGGCGGTGATTGCGCTGATGGGCCTGTGGCCGCTGATGCTGGCCATTGCGGTAGGCGTGAAG
ATGAGCTCGCCCGGCCCGGTGTTCTTCCGTCAGCGCCGCCACGGCCTGGGTGGCCGCGAGTTCTACATGTTCAAGTTCCG
CTCGATGCGGGTGCATGACGATCATGGCACCACGATTCAGCAGGCGACCAAGAACGACACGCGGATTACGCGCTTCGGCA
GTTTCCTGCGCCGCAGCAGCCTGGACGAGCTGCCGCAGATCTTCAATGTCTTGGGTGGCAGCATGTCGATCGTGGGCCCG
CGCCCGCACGCCGCGCAGCACAACACGCACTATGAAAAGCTGATCAACCATTACATGCAGCGTCACTACGTCAAGCCGGG
GATTACCGGTTGGGCGCAGGTCAACGGTTTCCGCGGTGAGACCCCGGAGCTGCGGACGATGAAGAAGCGCATCCAGTACG
ACCTTGACTACATCCGTCGTTGGTCGCTGTGGCTGGATATCCGCATCATCGTGCTGACGGCCGTGCGCGTGCTCGGACAG
AAGACCGCGTACTGA

Upstream 100 bases:

>100_bases
TAGGTATTACCGCGGTGATCGGACGACAGGATGATTGAAAGCTCGCGTGCGATTCGTATGTTCCCCCGCATGCGCCGTAT
CGAGTTTGGAGGACATCCCC

Downstream 100 bases:

>100_bases
TGACGGTGGGGAGTGTGCGACCTGGCGCACCTTGCGCCGCGGGCGGCTGCATCGCAGCCGCCTTTCTCTCGCGGGCGCTG
ACATGCTGATTCAAATGAGC

Product: GumD protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MLLADLSSATYTTSSPRLLSKYSAAADLVLRVFDLTMVVASGLIAYRIVFGTWVPAAPYRVAIATTLLYSVICFALFPLY
RSWRGRGLLSELMVLGGAFGGVFALFAVHALIVQVGEQVSRGWIGLWFVGGLVSLVAARTLLRGFLNHLRTQGVDVQRVV
VVGLRHPVMKISHYLSRNPWVGMNLVGYFRTPYDLAVTDQRQGLPCLGDPDELIEYLKNNQVEQVWISLPLGERDHIKQL
LQRLDRYPINVKLVPDLFDFGLLNQSAEQIGSVPVINLRQGGVDRDNYFVVAKALQDKILAVIALMGLWPLMLAIAVGVK
MSSPGPVFFRQRRHGLGGREFYMFKFRSMRVHDDHGTTIQQATKNDTRITRFGSFLRRSSLDELPQIFNVLGGSMSIVGP
RPHAAQHNTHYEKLINHYMQRHYVKPGITGWAQVNGFRGETPELRTMKKRIQYDLDYIRRWSLWLDIRIIVLTAVRVLGQ
KTAY

Sequences:

>Translated_484_residues
MLLADLSSATYTTSSPRLLSKYSAAADLVLRVFDLTMVVASGLIAYRIVFGTWVPAAPYRVAIATTLLYSVICFALFPLY
RSWRGRGLLSELMVLGGAFGGVFALFAVHALIVQVGEQVSRGWIGLWFVGGLVSLVAARTLLRGFLNHLRTQGVDVQRVV
VVGLRHPVMKISHYLSRNPWVGMNLVGYFRTPYDLAVTDQRQGLPCLGDPDELIEYLKNNQVEQVWISLPLGERDHIKQL
LQRLDRYPINVKLVPDLFDFGLLNQSAEQIGSVPVINLRQGGVDRDNYFVVAKALQDKILAVIALMGLWPLMLAIAVGVK
MSSPGPVFFRQRRHGLGGREFYMFKFRSMRVHDDHGTTIQQATKNDTRITRFGSFLRRSSLDELPQIFNVLGGSMSIVGP
RPHAAQHNTHYEKLINHYMQRHYVKPGITGWAQVNGFRGETPELRTMKKRIQYDLDYIRRWSLWLDIRIIVLTAVRVLGQ
KTAY
>Mature_484_residues
MLLADLSSATYTTSSPRLLSKYSAAADLVLRVFDLTMVVASGLIAYRIVFGTWVPAAPYRVAIATTLLYSVICFALFPLY
RSWRGRGLLSELMVLGGAFGGVFALFAVHALIVQVGEQVSRGWIGLWFVGGLVSLVAARTLLRGFLNHLRTQGVDVQRVV
VVGLRHPVMKISHYLSRNPWVGMNLVGYFRTPYDLAVTDQRQGLPCLGDPDELIEYLKNNQVEQVWISLPLGERDHIKQL
LQRLDRYPINVKLVPDLFDFGLLNQSAEQIGSVPVINLRQGGVDRDNYFVVAKALQDKILAVIALMGLWPLMLAIAVGVK
MSSPGPVFFRQRRHGLGGREFYMFKFRSMRVHDDHGTTIQQATKNDTRITRFGSFLRRSSLDELPQIFNVLGGSMSIVGP
RPHAAQHNTHYEKLINHYMQRHYVKPGITGWAQVNGFRGETPELRTMKKRIQYDLDYIRRWSLWLDIRIIVLTAVRVLGQ
KTAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=429, Percent_Identity=38.2284382284382, Blast_Score=292, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 54681; Mature: 54681

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLADLSSATYTTSSPRLLSKYSAAADLVLRVFDLTMVVASGLIAYRIVFGTWVPAAPYR
CEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
VAIATTLLYSVICFALFPLYRSWRGRGLLSELMVLGGAFGGVFALFAVHALIVQVGEQVS
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
RGWIGLWFVGGLVSLVAARTLLRGFLNHLRTQGVDVQRVVVVGLRHPVMKISHYLSRNPW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCC
VGMNLVGYFRTPYDLAVTDQRQGLPCLGDPDELIEYLKNNQVEQVWISLPLGERDHIKQL
CCCCHHEEECCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHH
LQRLDRYPINVKLVPDLFDFGLLNQSAEQIGSVPVINLRQGGVDRDNYFVVAKALQDKIL
HHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEHHHHHHHHH
AVIALMGLWPLMLAIAVGVKMSSPGPVFFRQRRHGLGGREFYMFKFRSMRVHDDHGTTIQ
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCHHH
QATKNDTRITRFGSFLRRSSLDELPQIFNVLGGSMSIVGPRPHAAQHNTHYEKLINHYMQ
HHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHH
RHYVKPGITGWAQVNGFRGETPELRTMKKRIQYDLDYIRRWSLWLDIRIIVLTAVRVLGQ
HHCCCCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTAY
CCCC
>Mature Secondary Structure
MLLADLSSATYTTSSPRLLSKYSAAADLVLRVFDLTMVVASGLIAYRIVFGTWVPAAPYR
CEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
VAIATTLLYSVICFALFPLYRSWRGRGLLSELMVLGGAFGGVFALFAVHALIVQVGEQVS
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
RGWIGLWFVGGLVSLVAARTLLRGFLNHLRTQGVDVQRVVVVGLRHPVMKISHYLSRNPW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCC
VGMNLVGYFRTPYDLAVTDQRQGLPCLGDPDELIEYLKNNQVEQVWISLPLGERDHIKQL
CCCCHHEEECCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHH
LQRLDRYPINVKLVPDLFDFGLLNQSAEQIGSVPVINLRQGGVDRDNYFVVAKALQDKIL
HHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEHHHHHHHHH
AVIALMGLWPLMLAIAVGVKMSSPGPVFFRQRRHGLGGREFYMFKFRSMRVHDDHGTTIQ
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCHHH
QATKNDTRITRFGSFLRRSSLDELPQIFNVLGGSMSIVGPRPHAAQHNTHYEKLINHYMQ
HHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHH
RHYVKPGITGWAQVNGFRGETPELRTMKKRIQYDLDYIRRWSLWLDIRIIVLTAVRVLGQ
HHCCCCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTAY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]