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The map label for this gene is pnp [H]

Identifier: 66767933

GI number: 66767933

Start: 1930836

End: 1932950

Strand: Direct

Name: pnp [H]

Synonym: XC_1609

Alternate gene names: 66767933

Gene position: 1930836-1932950 (Clockwise)

Preceding gene: 66767932

Following gene: 66767937

Centisome position: 37.5

GC content: 63.07

Gene sequence:

>2115_bases
GTGGCAAAAATCACCAAAACCTTCCAGTACGGCAAGCACACCGTCACCCTGGAGACGGGCGAAGTCGCTCGCCAGGCGAG
CGGTGCCGTCATCGTCAAGATGGACGACACCGTACTGCTGGTCACCGCCGTCGCCGCCAAGACCGCGCGCGAAGGGCAGG
ACTTCTTCCCCCTGACGGTTGACTATCAGGAGAAGTTCTATGCCGGCGGCCGCATCCCGGGCGGCTTCTTCAAGCGCGAA
GGACGTGCGACCGAGAAGGAAACGTTGATCTCGCGCCTGATCGATCGTCCGATCCGTCCGCTGTTCCCGGAGGACTACAA
GAACGAAGTGCAGATCATCGCCACGGTGATGTCGATGAACCCGGACATCGACGGCGATATCGCCGCGCTGATCGGTGCGT
CGGCTGCGCTGTCGTTGGCCGGTACCCCCTTCAACGGCCCGATCGGCGCCGCCAAGGTGGGCTACAAGAACGGCGAATAC
ATTCTCAACCCGACCATCACCGAGCTGAAGGATTCGCAGCTTGAGCTGGTTGTGGCCGGTACGGCGAACGCCGTGCTGAT
GGTGGAGTCGGAAGCGGCGCTGCTGTCGGAAGAAGTGATGCTGGGCGCGGTCACCTTTGGTCATCGCGAGATGCAGAAGG
TCATCAACATCATCAACGAACTGACCGTCGAAGCCGGCACCAAGCCCAGCACCTGGGTGGCCCCGGCCAAGAACACCGCG
CTGATCAGCGCGTTGAAGGAAGCGGTTGGCACCCAGCTGGCGGGTGCCTTCCAGGTGCGCGACAAGCTGCAGCGTCGCGA
CGCCATTTCGGCGATCAAGAAGGACGTGCTCGAGTCGCTGGCAGGCCGCGTGGCTTCCGAAGGCTGGAGCTCGGCCGAGC
TGTCGAAGGAATTCGGCGAGCTGGAATACCACACCATGCGTGACTCGGTGCTGGAGACCAAGGTGCGTATCGACGGCCGT
GCACTGGACACCGTGCGCCCGATCTCGGTACAGGCCGGCGTGCTGCCGCGGACCCATGGCTCGGCGCTGTTCACCCGCGG
CGAGACGCAGGCGATCGTGGTCACCACGCTGGGCACCGCCCGCGACGGCCAGGTGATCGACGCGGTCGCTGGCGAGTACA
AGGAAAACTTCCTGTTCCATTACAACTTCCCTCCGTATTCGGTGGGCGAGTGCGGCCGCTTTGGCGCGCCGAAGCGTCGC
GAGATCGGCCACGGCCGTCTGGCCAAGCGCGGCGTGTTGGCCGTGATGCCGAGCCTGGAAGAATTCCCGTACACCATCCG
CGTTGTCTCGGAAATCACCGAGTCCAACGGTTCCTCGTCGATGGCTTCGGTGTGCGGCTCCTCGCTCGCACTGATGGACG
CCGGCGTGCCGGTCAAGGCACCGGTGGCGGGTATCGCCATGGGCCTGGTGAAGGAAGACGACCGCTTCGTGGTGCTGTCC
GACATCCTGGGGGACGAAGATCACCTGGGCGACATGGACTTCAAGGTGGCCGGTACTGCTGAAGGCGTGTCCGCGCTGCA
GATGGACATCAAGATCGAAGGCATTACCGAAGAGATCATGAAGCAGGCACTGCAGCAGGCCAAGGCTGGCCGTCTGCACA
TCCTGGGCGAGATGTCCAAGGCGCTGACCACCCCGCGTCAGGAGCTGAGCGACTACGCGCCGCGCCTGCTGACCATCAAG
ATCCACCCGGACAAGATCCGCGAAGTGATCGGCAAGGGTGGCTCGACCATCCAGGCGATCACCAAGGAAACCGGGACGCA
GATCGATATCCAGGACGACGGCACGATCATCATTGCTTCGGTCAACGCGATTGCCGCGCAAGCTGCGAAGTCGCGCATCG
AGCAGATCACCTCGGACGTGGAGCCGGGCCGCATCTACGAAGGCAAGGTCGCCAAGATCATGGACTTCGGTGCGTTCGTG
ACCATCCTGCCGGGCAAGGATGGCCTGGTACACGTGTCGCAGATTTCCAGCGAGCGCGTGGAGAAGGTCGGCGACAAGTT
GAAGGAAGGCGATCTGGTCCGCGTCAAGGTGCTGGAAGTGGACAAGCAGGGCCGCATCCGTCTGTCGATCAAGGCAGTGG
AAGAAGGCGAAGGCGTGCCTGCATCGGCTGAGTAA

Upstream 100 bases:

>100_bases
GCGTCACATGCGTCATGGGCCGGAGCCTCCCGGCCGCCCGTTCTCGGCATGGGTCGACGACGGTCCAACGAAGACAGTTA
CACCTAAGGAAAAAACCTCC

Downstream 100 bases:

>100_bases
TCGCCGCTGCATGTGCGTTAGAAAAAGCGGGCTTCGGCCCGCTTTTTTGTTGGGCGAAGTACGGTGCAGTACAGCGTTTG
TTTAGGGTGTTGTTGTGCAC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MAKITKTFQYGKHTVTLETGEVARQASGAVIVKMDDTVLLVTAVAAKTAREGQDFFPLTVDYQEKFYAGGRIPGGFFKRE
GRATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMNPDIDGDIAALIGASAALSLAGTPFNGPIGAAKVGYKNGEY
ILNPTITELKDSQLELVVAGTANAVLMVESEAALLSEEVMLGAVTFGHREMQKVINIINELTVEAGTKPSTWVAPAKNTA
LISALKEAVGTQLAGAFQVRDKLQRRDAISAIKKDVLESLAGRVASEGWSSAELSKEFGELEYHTMRDSVLETKVRIDGR
ALDTVRPISVQAGVLPRTHGSALFTRGETQAIVVTTLGTARDGQVIDAVAGEYKENFLFHYNFPPYSVGECGRFGAPKRR
EIGHGRLAKRGVLAVMPSLEEFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPVKAPVAGIAMGLVKEDDRFVVLS
DILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMSKALTTPRQELSDYAPRLLTIK
IHPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIASVNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFV
TILPGKDGLVHVSQISSERVEKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVPASAE

Sequences:

>Translated_704_residues
MAKITKTFQYGKHTVTLETGEVARQASGAVIVKMDDTVLLVTAVAAKTAREGQDFFPLTVDYQEKFYAGGRIPGGFFKRE
GRATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMNPDIDGDIAALIGASAALSLAGTPFNGPIGAAKVGYKNGEY
ILNPTITELKDSQLELVVAGTANAVLMVESEAALLSEEVMLGAVTFGHREMQKVINIINELTVEAGTKPSTWVAPAKNTA
LISALKEAVGTQLAGAFQVRDKLQRRDAISAIKKDVLESLAGRVASEGWSSAELSKEFGELEYHTMRDSVLETKVRIDGR
ALDTVRPISVQAGVLPRTHGSALFTRGETQAIVVTTLGTARDGQVIDAVAGEYKENFLFHYNFPPYSVGECGRFGAPKRR
EIGHGRLAKRGVLAVMPSLEEFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPVKAPVAGIAMGLVKEDDRFVVLS
DILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMSKALTTPRQELSDYAPRLLTIK
IHPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIASVNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFV
TILPGKDGLVHVSQISSERVEKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVPASAE
>Mature_703_residues
AKITKTFQYGKHTVTLETGEVARQASGAVIVKMDDTVLLVTAVAAKTAREGQDFFPLTVDYQEKFYAGGRIPGGFFKREG
RATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMNPDIDGDIAALIGASAALSLAGTPFNGPIGAAKVGYKNGEYI
LNPTITELKDSQLELVVAGTANAVLMVESEAALLSEEVMLGAVTFGHREMQKVINIINELTVEAGTKPSTWVAPAKNTAL
ISALKEAVGTQLAGAFQVRDKLQRRDAISAIKKDVLESLAGRVASEGWSSAELSKEFGELEYHTMRDSVLETKVRIDGRA
LDTVRPISVQAGVLPRTHGSALFTRGETQAIVVTTLGTARDGQVIDAVAGEYKENFLFHYNFPPYSVGECGRFGAPKRRE
IGHGRLAKRGVLAVMPSLEEFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPVKAPVAGIAMGLVKEDDRFVVLSD
ILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMSKALTTPRQELSDYAPRLLTIKI
HPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIASVNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFVT
ILPGKDGLVHVSQISSERVEKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVPASAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=709, Percent_Identity=36.5303244005642, Blast_Score=431, Evalue=1e-120,
Organism=Escherichia coli, GI145693187, Length=695, Percent_Identity=64.6043165467626, Blast_Score=894, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=82, Percent_Identity=46.3414634146341, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI115534063, Length=721, Percent_Identity=33.1484049930652, Blast_Score=360, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17535281, Length=81, Percent_Identity=46.9135802469136, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281362905, Length=728, Percent_Identity=38.3241758241758, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24651641, Length=728, Percent_Identity=38.3241758241758, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24651643, Length=728, Percent_Identity=38.3241758241758, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI161079377, Length=668, Percent_Identity=37.874251497006, Blast_Score=420, Evalue=1e-117,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 75672; Mature: 75541

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKITKTFQYGKHTVTLETGEVARQASGAVIVKMDDTVLLVTAVAAKTAREGQDFFPLTV
CCCCCCHHHCCCEEEEEECHHHHHHCCCEEEEEECCCEEEEEHHHHHHHHCCCCCCEEEE
DYQEKFYAGGRIPGGFFKREGRATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMN
CCCHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
PDIDGDIAALIGASAALSLAGTPFNGPIGAAKVGYKNGEYILNPTITELKDSQLELVVAG
CCCCCCHHHHHCCHHHEEECCCCCCCCCCCCEECCCCCCEEECCCHHHCCCCCEEEEEEC
TANAVLMVESEAALLSEEVMLGAVTFGHREMQKVINIINELTVEAGTKPSTWVAPAKNTA
CCCEEEEEECCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHH
LISALKEAVGTQLAGAFQVRDKLQRRDAISAIKKDVLESLAGRVASEGWSSAELSKEFGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
LEYHTMRDSVLETKVRIDGRALDTVRPISVQAGVLPRTHGSALFTRGETQAIVVTTLGTA
CHHHHHHHHHHHHHEEECCEEECCCCCCEEECCCCCCCCCCEEEECCCCCEEEEEEECCC
RDGQVIDAVAGEYKENFLFHYNFPPYSVGECGRFGAPKRREIGHGRLAKRGVLAVMPSLE
CCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHEECCCHH
EFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPVKAPVAGIAMGLVKEDDRFVVLS
HCCCHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHEECCCCEEEEH
DILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMSK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCEEEEHHHHH
ALTTPRQELSDYAPRLLTIKIHPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIAS
HHCCCHHHHHHCCCEEEEEEECHHHHHHHHCCCCCEEEHHHHCCCCEEEEECCCEEEEEE
VNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFVTILPGKDGLVHVSQISSERV
CHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHEECCCEEEEECCCCCEEEHHHHHHHHH
EKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVPASAE
HHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
AKITKTFQYGKHTVTLETGEVARQASGAVIVKMDDTVLLVTAVAAKTAREGQDFFPLTV
CCCCCHHHCCCEEEEEECHHHHHHCCCEEEEEECCCEEEEEHHHHHHHHCCCCCCEEEE
DYQEKFYAGGRIPGGFFKREGRATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMN
CCCHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
PDIDGDIAALIGASAALSLAGTPFNGPIGAAKVGYKNGEYILNPTITELKDSQLELVVAG
CCCCCCHHHHHCCHHHEEECCCCCCCCCCCCEECCCCCCEEECCCHHHCCCCCEEEEEEC
TANAVLMVESEAALLSEEVMLGAVTFGHREMQKVINIINELTVEAGTKPSTWVAPAKNTA
CCCEEEEEECCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHH
LISALKEAVGTQLAGAFQVRDKLQRRDAISAIKKDVLESLAGRVASEGWSSAELSKEFGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
LEYHTMRDSVLETKVRIDGRALDTVRPISVQAGVLPRTHGSALFTRGETQAIVVTTLGTA
CHHHHHHHHHHHHHEEECCEEECCCCCCEEECCCCCCCCCCEEEECCCCCEEEEEEECCC
RDGQVIDAVAGEYKENFLFHYNFPPYSVGECGRFGAPKRREIGHGRLAKRGVLAVMPSLE
CCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHEECCCHH
EFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPVKAPVAGIAMGLVKEDDRFVVLS
HCCCHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHEECCCCEEEEH
DILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMSK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCEEEEHHHHH
ALTTPRQELSDYAPRLLTIKIHPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIAS
HHCCCHHHHHHCCCEEEEEEECHHHHHHHHCCCCCEEEHHHHCCCCEEEEECCCEEEEEE
VNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFVTILPGKDGLVHVSQISSERV
CHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHEECCCEEEEECCCCCEEEHHHHHHHHH
EKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVPASAE
HHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]