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The map label for this gene is rluF [H]

Identifier: 66767803

GI number: 66767803

Start: 1778251

End: 1778973

Strand: Direct

Name: rluF [H]

Synonym: XC_1477

Alternate gene names: 66767803

Gene position: 1778251-1778973 (Clockwise)

Preceding gene: 66767802

Following gene: 66767806

Centisome position: 34.54

GC content: 64.45

Gene sequence:

>723_bases
ATGACGACCCGACTCAACAAACATATCGCCGAAACCGGCTTTTGCTCCCGCCGCGAGGCGGACCGCCTGATCGGCGAACG
CCGCGTCACCGTCAATGGCGTGATCGCCGGCACCGGCGCGGTGGTCGGCGAAGACGACACCGTGCTGGTGGACGGCCAGC
CCCTGCGCGTGCGCGAGGCCAAAAAGCCTGGTACGCGCCGCCACGTGTACATCGCACTCAACAAGCCAGTGGGCATCACC
AGCACCACCGAGAGTTCGGTGAAGGGCAACATCGTGGAATTCGTGGGCCACGAGCAGCGCATCTTCCCGATCGGCCGGCT
GGACAAGGACTCCGAAGGGCTGATCCTGATGACCAGCAACGGCAACATCGTCAACGAGATCCTGCGCGCGGAAAATCGGC
ACCAGAAGGAATACCTGGTGGCGGTCAACAAGCCGGTCACCGACGAATTCCTGCGCGGCATGGCCCGCGGCGTGCGCCTG
CATGACGAAACCACCTTGCCGTGCCGTACCGCGCGCATCGCCAAGTTCGGTTTCCGCATCGTGCTGGAGCAGGGCTTGAA
CCGGCAGATCCGCCTGATGGCCGCGGCGTTCGGCTTCCGCGTGACCCAGCTGCGCCGCGTGCGCATCGACAACATCAAGC
TGGCCGCGCTCAAGCCCGGCCAGTGGCGCAATCTCACCGACCAGGAATTGCAGGGGTTATTGCCGCAGCGTACTGATTGG
TGA

Upstream 100 bases:

>100_bases
ACGACCTGCTGCATGTGTCGCTACCCAGCCTGGCTGCCTCCTGAGCGGCCCTGACGCACGCAATGGCCCGCGCGGCGCCG
CAATCGCCGATAATCCGCCG

Downstream 100 bases:

>100_bases
GTGTGGCTTGGCAGTGTGTTGGGGCGGTCGTTGCTTGGCGCAGTTTGATCGTCCCTCGGACGCTTCCCGATGTCGCCCCT
GAAAAACCCCCTTCAAGCAC

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTTRLNKHIAETGFCSRREADRLIGERRVTVNGVIAGTGAVVGEDDTVLVDGQPLRVREAKKPGTRRHVYIALNKPVGIT
STTESSVKGNIVEFVGHEQRIFPIGRLDKDSEGLILMTSNGNIVNEILRAENRHQKEYLVAVNKPVTDEFLRGMARGVRL
HDETTLPCRTARIAKFGFRIVLEQGLNRQIRLMAAAFGFRVTQLRRVRIDNIKLAALKPGQWRNLTDQELQGLLPQRTDW

Sequences:

>Translated_240_residues
MTTRLNKHIAETGFCSRREADRLIGERRVTVNGVIAGTGAVVGEDDTVLVDGQPLRVREAKKPGTRRHVYIALNKPVGIT
STTESSVKGNIVEFVGHEQRIFPIGRLDKDSEGLILMTSNGNIVNEILRAENRHQKEYLVAVNKPVTDEFLRGMARGVRL
HDETTLPCRTARIAKFGFRIVLEQGLNRQIRLMAAAFGFRVTQLRRVRIDNIKLAALKPGQWRNLTDQELQGLLPQRTDW
>Mature_239_residues
TTRLNKHIAETGFCSRREADRLIGERRVTVNGVIAGTGAVVGEDDTVLVDGQPLRVREAKKPGTRRHVYIALNKPVGITS
TTESSVKGNIVEFVGHEQRIFPIGRLDKDSEGLILMTSNGNIVNEILRAENRHQKEYLVAVNKPVTDEFLRGMARGVRLH
DETTLPCRTARIAKFGFRIVLEQGLNRQIRLMAAAFGFRVTQLRRVRIDNIKLAALKPGQWRNLTDQELQGLLPQRTDW

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=53.6480686695279, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=35.7142857142857, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787524, Length=241, Percent_Identity=31.1203319502075, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081838, Length=187, Percent_Identity=30.4812834224599, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27027; Mature: 26895

Theoretical pI: Translated: 10.96; Mature: 10.96

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRLNKHIAETGFCSRREADRLIGERRVTVNGVIAGTGAVVGEDDTVLVDGQPLRVREA
CCCCHHHHHHHCCCCCHHHHHHHHCCCEEEEEEEEEECCEEECCCCEEEECCCCEEEECC
KKPGTRRHVYIALNKPVGITSTTESSVKGNIVEFVGHEQRIFPIGRLDKDSEGLILMTSN
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHEECCCCCEEEECCCCCCCCCCEEEEECC
GNIVNEILRAENRHQKEYLVAVNKPVTDEFLRGMARGVRLHDETTLPCRTARIAKFGFRI
CHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHH
VLEQGLNRQIRLMAAAFGFRVTQLRRVRIDNIKLAALKPGQWRNLTDQELQGLLPQRTDW
EEHHCCCHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTRLNKHIAETGFCSRREADRLIGERRVTVNGVIAGTGAVVGEDDTVLVDGQPLRVREA
CCCHHHHHHHCCCCCHHHHHHHHCCCEEEEEEEEEECCEEECCCCEEEECCCCEEEECC
KKPGTRRHVYIALNKPVGITSTTESSVKGNIVEFVGHEQRIFPIGRLDKDSEGLILMTSN
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHEECCCCCEEEECCCCCCCCCCEEEEECC
GNIVNEILRAENRHQKEYLVAVNKPVTDEFLRGMARGVRLHDETTLPCRTARIAKFGFRI
CHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHH
VLEQGLNRQIRLMAAAFGFRVTQLRRVRIDNIKLAALKPGQWRNLTDQELQGLLPQRTDW
EEHHCCCHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]