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The map label for this gene is dosP [H]

Identifier: 66767737

GI number: 66767737

Start: 1701644

End: 1702927

Strand: Reverse

Name: dosP [H]

Synonym: XC_1411

Alternate gene names: 66767737

Gene position: 1702927-1701644 (Counterclockwise)

Preceding gene: 66767738

Following gene: 66767736

Centisome position: 33.07

GC content: 68.85

Gene sequence:

>1284_bases
ATGCACTCTCCACTCCTTTCCTGCAACTGCGCACTGGCCGGCCCGGTCCTGGTGGTCGACGACAGCGTGGTCCAGCGCGA
ACACGCCATGGCGCTGTGCCGCCAATTGGGCGCCAGCGTGGTGGAAGGTGCCGGCGACGGCCATGCCGCGCTCGACTGGC
TGAGCCGCGCCAGCGCACCATCGCTGCTGTTGATCGACCTGGAAATGCCGGGAATGGACGGCGTGCAGCTGCTCGATGCG
CTGGCACGCGGCCAGCACAGCGTGCCGGTGGTGGTGGTCTCGCAGCGCGGCGGCGCGCTGATCGATGCGGTGATGCAACT
CAGCCGCAGCGCCGGTGTGCGCGTGCTCGCCGGCCTGGAAAAACCGCTGCGCCTGCAGGACCTGGCCACCACGCTGGACT
GCATTCCCGAACCGCTCGCCGAGACCACCACCGCGCTGTCCACGCCGCTCTCGCCGCTGCTCTCCAGTGGCGGCGCGGCG
GCCTCGCGGCTGGATCGCGCCATGCGCCGCGGCGAAATCCGCGTGGCTTATCAGCCCAAGCTGGACCTGCGCGACGGCCG
CCTGCGCGGCGTTGAGGCCCTGGCGCGCTGGCGCCGCCCGCAAGGCGACATGATCGGCCCGGACCGCTTCATTCCCTTGG
CCGAGCGCGAAGGTTTGATCCACGCACTCACCCAGCAGGTGATCGACAAGGCGGTTGCGCAACTGGTCGACTGGCGCGCC
GAAGGCCTGCAACTCACGCTGGCCTTGAACCTGTCGCCCAATCTGCTGGGCGAGCAGGATTTTCTCGAACACCTGTGCGC
CAAGCTGGCCGACAACGGCCTGGCACCGGCCGACCTGGTGCTGGAACTCACCGAAAGCGCCATCGTGGAGCCGGCCAATG
CGCTGAGCATGCTGGCGCGGCTGCGCCTGCATGGGTTCGGCCTGTCCATCGACGATTACGGCACCGGCTTTTCCTCGTTG
CAGCGGCTGGCCAGCATCCCGTTTACCGAGCTCAAACTCGACCGCAGCTTCGTGCATGCCGCGCACCGCAGCCGCAGCCA
GCGCACCGTGCTGGAATCCACGCTGGAGCTGGCCCACCGGCTGGAACTCACTGCCGTGGCCGAGGGCGTGGAAACCCCGG
AGGACTGGCGCCTGCTCCGCGAACTCGGCTGCGACCTGGCCCAGGGCTACCTGATGGCCTCCCCGATGCCGGGCCCGATG
TTGTCGGAGTGGTGGCGCGAACACGCCAGCCGCATCGCCCGTCTCTGCAGCAGCGAGCTTCCCACTGATGCCGACCTTGT
CTGA

Upstream 100 bases:

>100_bases
TCCTGCTGGATGCCGACGCCGTGCTGGCCAACGACGCGCCGGCGCAGCTGCCCGACGCCGCGCTGGCGGCCTGACTCTTT
CCGTGCACGCCAGGACTCTC

Downstream 100 bases:

>100_bases
GCCCATGAGCACGTCGACCGCGATCACCGAACAGGAATTCGGGCGCTTTCAGCGCTTCATTTTCGAAGCCGCCGGCATCA
GCATTTCTTCCGGCAAGAAG

Product: response regulator

Products: NA

Alternate protein names: Direct oxygen sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase, [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MHSPLLSCNCALAGPVLVVDDSVVQREHAMALCRQLGASVVEGAGDGHAALDWLSRASAPSLLLIDLEMPGMDGVQLLDA
LARGQHSVPVVVVSQRGGALIDAVMQLSRSAGVRVLAGLEKPLRLQDLATTLDCIPEPLAETTTALSTPLSPLLSSGGAA
ASRLDRAMRRGEIRVAYQPKLDLRDGRLRGVEALARWRRPQGDMIGPDRFIPLAEREGLIHALTQQVIDKAVAQLVDWRA
EGLQLTLALNLSPNLLGEQDFLEHLCAKLADNGLAPADLVLELTESAIVEPANALSMLARLRLHGFGLSIDDYGTGFSSL
QRLASIPFTELKLDRSFVHAAHRSRSQRTVLESTLELAHRLELTAVAEGVETPEDWRLLRELGCDLAQGYLMASPMPGPM
LSEWWREHASRIARLCSSELPTDADLV

Sequences:

>Translated_427_residues
MHSPLLSCNCALAGPVLVVDDSVVQREHAMALCRQLGASVVEGAGDGHAALDWLSRASAPSLLLIDLEMPGMDGVQLLDA
LARGQHSVPVVVVSQRGGALIDAVMQLSRSAGVRVLAGLEKPLRLQDLATTLDCIPEPLAETTTALSTPLSPLLSSGGAA
ASRLDRAMRRGEIRVAYQPKLDLRDGRLRGVEALARWRRPQGDMIGPDRFIPLAEREGLIHALTQQVIDKAVAQLVDWRA
EGLQLTLALNLSPNLLGEQDFLEHLCAKLADNGLAPADLVLELTESAIVEPANALSMLARLRLHGFGLSIDDYGTGFSSL
QRLASIPFTELKLDRSFVHAAHRSRSQRTVLESTLELAHRLELTAVAEGVETPEDWRLLRELGCDLAQGYLMASPMPGPM
LSEWWREHASRIARLCSSELPTDADLV
>Mature_427_residues
MHSPLLSCNCALAGPVLVVDDSVVQREHAMALCRQLGASVVEGAGDGHAALDWLSRASAPSLLLIDLEMPGMDGVQLLDA
LARGQHSVPVVVVSQRGGALIDAVMQLSRSAGVRVLAGLEKPLRLQDLATTLDCIPEPLAETTTALSTPLSPLLSSGGAA
ASRLDRAMRRGEIRVAYQPKLDLRDGRLRGVEALARWRRPQGDMIGPDRFIPLAEREGLIHALTQQVIDKAVAQLVDWRA
EGLQLTLALNLSPNLLGEQDFLEHLCAKLADNGLAPADLVLELTESAIVEPANALSMLARLRLHGFGLSIDDYGTGFSSL
QRLASIPFTELKLDRSFVHAAHRSRSQRTVLESTLELAHRLELTAVAEGVETPEDWRLLRELGCDLAQGYLMASPMPGPM
LSEWWREHASRIARLCSSELPTDADLV

Specific function: In association with DosC is involved in the production and removal of the second messenger c-di-GMP in response to changing O(2) levels. Has phosphodiesterase (PDE) activity with c- di-GMP (PubMed:15995192), very poor activity on cAMP (PubMed:15995192) bu

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=354, Percent_Identity=31.0734463276836, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1790496, Length=242, Percent_Identity=33.4710743801653, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787541, Length=251, Percent_Identity=34.2629482071713, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI87081743, Length=246, Percent_Identity=33.739837398374, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87081845, Length=241, Percent_Identity=36.9294605809129, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788502, Length=244, Percent_Identity=35.2459016393443, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787055, Length=248, Percent_Identity=33.0645161290323, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1786507, Length=242, Percent_Identity=34.297520661157, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI87081980, Length=241, Percent_Identity=31.9502074688797, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI226510982, Length=247, Percent_Identity=31.5789473684211, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788849, Length=240, Percent_Identity=30, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082096, Length=233, Percent_Identity=27.8969957081545, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1787410, Length=235, Percent_Identity=25.1063829787234, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR012226
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00989 PAS; PF08448 PAS_4 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 46171; Mature: 46171

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSPLLSCNCALAGPVLVVDDSVVQREHAMALCRQLGASVVEGAGDGHAALDWLSRASAP
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
SLLLIDLEMPGMDGVQLLDALARGQHSVPVVVVSQRGGALIDAVMQLSRSAGVRVLAGLE
CEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEECCCC
KPLRLQDLATTLDCIPEPLAETTTALSTPLSPLLSSGGAAASRLDRAMRRGEIRVAYQPK
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHCCCEEEEECCC
LDLRDGRLRGVEALARWRRPQGDMIGPDRFIPLAEREGLIHALTQQVIDKAVAQLVDWRA
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHC
EGLQLTLALNLSPNLLGEQDFLEHLCAKLADNGLAPADLVLELTESAIVEPANALSMLAR
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LRLHGFGLSIDDYGTGFSSLQRLASIPFTELKLDRSFVHAAHRSRSQRTVLESTLELAHR
HHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELTAVAEGVETPEDWRLLRELGCDLAQGYLMASPMPGPMLSEWWREHASRIARLCSSEL
HHHHHHHHCCCCCHHHHHHHHHCCHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
PTDADLV
CCCCCCC
>Mature Secondary Structure
MHSPLLSCNCALAGPVLVVDDSVVQREHAMALCRQLGASVVEGAGDGHAALDWLSRASAP
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
SLLLIDLEMPGMDGVQLLDALARGQHSVPVVVVSQRGGALIDAVMQLSRSAGVRVLAGLE
CEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEECCCC
KPLRLQDLATTLDCIPEPLAETTTALSTPLSPLLSSGGAAASRLDRAMRRGEIRVAYQPK
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHCCCEEEEECCC
LDLRDGRLRGVEALARWRRPQGDMIGPDRFIPLAEREGLIHALTQQVIDKAVAQLVDWRA
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHC
EGLQLTLALNLSPNLLGEQDFLEHLCAKLADNGLAPADLVLELTESAIVEPANALSMLAR
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LRLHGFGLSIDDYGTGFSSLQRLASIPFTELKLDRSFVHAAHRSRSQRTVLESTLELAHR
HHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELTAVAEGVETPEDWRLLRELGCDLAQGYLMASPMPGPMLSEWWREHASRIARLCSSEL
HHHHHHHHCCCCCHHHHHHHHHCCHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
PTDADLV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]