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The map label for this gene is mcrB [H]

Identifier: 66767707

GI number: 66767707

Start: 1665787

End: 1668315

Strand: Direct

Name: mcrB [H]

Synonym: XC_1380

Alternate gene names: 66767707

Gene position: 1665787-1668315 (Clockwise)

Preceding gene: 66767706

Following gene: 66767708

Centisome position: 32.35

GC content: 56.58

Gene sequence:

>2529_bases
ATGGCCTTCAGCACGGACGACACTTTGTGGCGTGAGTTTCTTCAGGTGTGGCCGCTGGAGCGGTTACGCGAGATGACTCT
GGCCGAGTACACCACGGCGGGAGACAAAGACTGTTTTGTCTACTGGCTGGAGTTCCGGCTGGTTGATTACGGCAGTATCG
CCGGGGGGTCCGCTTTCAAATTTGCAATCTTTTCCCGCAAGGAAACGGGGGCGCGTGAGGGCGATACGTCCTTGGCTTAC
GACGACCGCTACGGCTGGTACCGCCGATTCGGCACATCTCCCGAAGAGGCATTTCAGACCATTCGTGGACATGTGGTGGC
CGTTGCTGAGGCTGCTCGTGACGGTCGACTGGACGAAATCGACAACAGCCCGCTTGGCGCTGCCTACCGCTGGAAGATCG
CCTTTCACTATCAGCCGCTGCGAAACCCGAGTACTCCTTGCGTCTACGTGCGTAAGCCTCTTTTGCACTTTCTCGGCCTA
TCGGCTGCGGACACTACAACACCTCAAAGTGAGCTTTACCAAGCCCTCGCAAGACTGCGTCAGGCGGATGAGAGTATCTT
CGTGTTCAGTGAACGGCTATGGAAAGACTGGGTCGCCGACACACCGTTTGTCGTCAAGCTGAGCGAGGGTGCAATCAAGC
ACGGCTATCTCCCGGTCAATCTCATCTCCGCCCCCTTTCCGGAGACCATGTACGGCGGCAAGACACCGAATGAAGCAGCC
GACGTGGCGCATTTCCGCACCGATACAGGTCTGGAGTTCGACACCGACCTACGCGTCTCTAGCCCCGGTAGCGCTCGTCT
GCGTCACAGACTGGGCGGTTATTTCGAAGACATTGGTGCAGCGGCCGGGGACCTCATCACTTTGACTCAGGATGGCGATG
GGACCTACCTTTTGTCCTCAAAGTTAGCGGTCCATGCGATCGGTCCGACTGCGTCTGCGGCAACAATCACCGCACGGGCA
TCAGTGAAGGAGGCTGCCGCCGTGCGCCCCCCTTTAAACCAGATCCTTTTCGGACCGCCGGGGACAGGAAAGACCTACGA
GGCTATCGAGAAGGCTTTGGAGATTCTCGACCCTTCCTTTCTCTTGCAGTTGGGCGATGACGAGAGCAACGAGCGCCGGA
GGGCGCTCAAACGGCGCTATGACGAACTCGCGAAGGAAGGCCTTGTGCAGTTCGTAACGTTCCACCAGAGCTTCAGCTAC
GAAAACTTCGTTGAGGGCTTGAGCGCCAGCTCCGATCCGGATACCAAGCAGGTCCGCTATGAGGTGGAAGACGGTGTCTT
CAAGGAGCTTTGCGAGTCTGCGCGCACCCGACTGGTCCGCAGGAGCGATGACGCCCCACTGGAACTCTCGGGCCGGCGCA
TCTGGAAGCTTTCGCTGGGCGACTCGATCACTGAGGGACACATCTTCGAGGAGTGCATGGAGAAAGGTCTTGCCCTGATG
GGCTTTGGCAACAACGCAGACTTTTCGCAGTGCCAGTCCCGCGAAGATATCCAGAAGATGTTTGCTGCCTCGGGTGAACC
CCTCTCGCTTGGCGCCTATCCAATCACAGCGATCAAGACGTTTGTCTGCCAGATGAAGTTGGGTGATCTCATTGTCGTCA
GTGAAGGCAACTTGAAATTCCGCGCCATTGGCGAGATTACCGGCGACTATCAGTTGCTGCCACGGGATAGTGACACCTAT
TCACAAGCGAGGCCTGTTCGCTGGTTGCGCGGCTACAAGCCAGCGTTACCGTACGGCGCGCTGATGGAAAATCGGTTCAG
CCAGATGACCGTGTACGAACTTCGAGCGGGATCGATTGACCACGACAAGTTTCGGGAGCTCCTGAAATCCGAATGCATCG
CGACCGGTGCTGACAGACCACACGTGTTGATCATCGATGAGATTAATCGAGGTAACGTCTCGCGAATCTTCGGCGAGTTG
ATTACGCTGATTGAGCCATCTAAGCGCTCAGGTGCTGCCGAGGCACTGGAGGTATTGCTGCCCTACTCGAAAGATCCATT
CAGCGTGCCGGACAACGTGTATCTGATCGGGACCATGAACACGGCAGACCGTTCATTGGCCGGCCTCGACATCGCACTTC
GTCGACGCTTCGACTTTGTGGAGATGGCACCCAGGCCGGACAAGCTTACCGGGGTCAAAGTTGCCGAAATCGACATTTCG
GCTGTACTTGAGACGATGAATCGGCGTATCGAGGTCTTGCTCGATCGCGAGCACCGGCTTGGGCATGCCTATTTTATGTC
GCTTGAAAACAGCAACGACCTTTCGAGGTTGTCGAATATCTTTCGGCAGCAAATCATCCCGCTGCTTCAGGAATACTTCT
TCGAGGACTGGCAGCGCATTGCGTGGATACTTAACGATCATCGCAAAGACGAGCGATTCCGTTTCATCGTACCCGCCCCT
TATTCGGTGGACTCGTTGTTCGGCGATACCGGTCAGGTAGCACAGGATTCCAAGGCATGGCGAATGAACGAAGAAGCCTT
TCACAAGCCGCAGAGCTATCTAGGCATCGTCGGTGACGGCAAGGTCTGA

Upstream 100 bases:

>100_bases
CGAGCTGCAAACGAGGCTGGGTTGGACGTGCCAGAACGATGTGGTCCCGCTCGGCCACCCACCTCGCACGATGCGACAAA
CCACAATGGAGCTGTGACCC

Downstream 100 bases:

>100_bases
GCCGGTGCGTAAGCTAGTTGTCGTGAGGGAGTACGCGCGGTTAACCACGTCCGCCGTTACCGATGAATCTCTCGACGTTG
CCCGCGTACCGGAATCAGCG

Product: McrB-related protein

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 842; Mature: 841

Protein sequence:

>842_residues
MAFSTDDTLWREFLQVWPLERLREMTLAEYTTAGDKDCFVYWLEFRLVDYGSIAGGSAFKFAIFSRKETGAREGDTSLAY
DDRYGWYRRFGTSPEEAFQTIRGHVVAVAEAARDGRLDEIDNSPLGAAYRWKIAFHYQPLRNPSTPCVYVRKPLLHFLGL
SAADTTTPQSELYQALARLRQADESIFVFSERLWKDWVADTPFVVKLSEGAIKHGYLPVNLISAPFPETMYGGKTPNEAA
DVAHFRTDTGLEFDTDLRVSSPGSARLRHRLGGYFEDIGAAAGDLITLTQDGDGTYLLSSKLAVHAIGPTASAATITARA
SVKEAAAVRPPLNQILFGPPGTGKTYEAIEKALEILDPSFLLQLGDDESNERRRALKRRYDELAKEGLVQFVTFHQSFSY
ENFVEGLSASSDPDTKQVRYEVEDGVFKELCESARTRLVRRSDDAPLELSGRRIWKLSLGDSITEGHIFEECMEKGLALM
GFGNNADFSQCQSREDIQKMFAASGEPLSLGAYPITAIKTFVCQMKLGDLIVVSEGNLKFRAIGEITGDYQLLPRDSDTY
SQARPVRWLRGYKPALPYGALMENRFSQMTVYELRAGSIDHDKFRELLKSECIATGADRPHVLIIDEINRGNVSRIFGEL
ITLIEPSKRSGAAEALEVLLPYSKDPFSVPDNVYLIGTMNTADRSLAGLDIALRRRFDFVEMAPRPDKLTGVKVAEIDIS
AVLETMNRRIEVLLDREHRLGHAYFMSLENSNDLSRLSNIFRQQIIPLLQEYFFEDWQRIAWILNDHRKDERFRFIVPAP
YSVDSLFGDTGQVAQDSKAWRMNEEAFHKPQSYLGIVGDGKV

Sequences:

>Translated_842_residues
MAFSTDDTLWREFLQVWPLERLREMTLAEYTTAGDKDCFVYWLEFRLVDYGSIAGGSAFKFAIFSRKETGAREGDTSLAY
DDRYGWYRRFGTSPEEAFQTIRGHVVAVAEAARDGRLDEIDNSPLGAAYRWKIAFHYQPLRNPSTPCVYVRKPLLHFLGL
SAADTTTPQSELYQALARLRQADESIFVFSERLWKDWVADTPFVVKLSEGAIKHGYLPVNLISAPFPETMYGGKTPNEAA
DVAHFRTDTGLEFDTDLRVSSPGSARLRHRLGGYFEDIGAAAGDLITLTQDGDGTYLLSSKLAVHAIGPTASAATITARA
SVKEAAAVRPPLNQILFGPPGTGKTYEAIEKALEILDPSFLLQLGDDESNERRRALKRRYDELAKEGLVQFVTFHQSFSY
ENFVEGLSASSDPDTKQVRYEVEDGVFKELCESARTRLVRRSDDAPLELSGRRIWKLSLGDSITEGHIFEECMEKGLALM
GFGNNADFSQCQSREDIQKMFAASGEPLSLGAYPITAIKTFVCQMKLGDLIVVSEGNLKFRAIGEITGDYQLLPRDSDTY
SQARPVRWLRGYKPALPYGALMENRFSQMTVYELRAGSIDHDKFRELLKSECIATGADRPHVLIIDEINRGNVSRIFGEL
ITLIEPSKRSGAAEALEVLLPYSKDPFSVPDNVYLIGTMNTADRSLAGLDIALRRRFDFVEMAPRPDKLTGVKVAEIDIS
AVLETMNRRIEVLLDREHRLGHAYFMSLENSNDLSRLSNIFRQQIIPLLQEYFFEDWQRIAWILNDHRKDERFRFIVPAP
YSVDSLFGDTGQVAQDSKAWRMNEEAFHKPQSYLGIVGDGKV
>Mature_841_residues
AFSTDDTLWREFLQVWPLERLREMTLAEYTTAGDKDCFVYWLEFRLVDYGSIAGGSAFKFAIFSRKETGAREGDTSLAYD
DRYGWYRRFGTSPEEAFQTIRGHVVAVAEAARDGRLDEIDNSPLGAAYRWKIAFHYQPLRNPSTPCVYVRKPLLHFLGLS
AADTTTPQSELYQALARLRQADESIFVFSERLWKDWVADTPFVVKLSEGAIKHGYLPVNLISAPFPETMYGGKTPNEAAD
VAHFRTDTGLEFDTDLRVSSPGSARLRHRLGGYFEDIGAAAGDLITLTQDGDGTYLLSSKLAVHAIGPTASAATITARAS
VKEAAAVRPPLNQILFGPPGTGKTYEAIEKALEILDPSFLLQLGDDESNERRRALKRRYDELAKEGLVQFVTFHQSFSYE
NFVEGLSASSDPDTKQVRYEVEDGVFKELCESARTRLVRRSDDAPLELSGRRIWKLSLGDSITEGHIFEECMEKGLALMG
FGNNADFSQCQSREDIQKMFAASGEPLSLGAYPITAIKTFVCQMKLGDLIVVSEGNLKFRAIGEITGDYQLLPRDSDTYS
QARPVRWLRGYKPALPYGALMENRFSQMTVYELRAGSIDHDKFRELLKSECIATGADRPHVLIIDEINRGNVSRIFGELI
TLIEPSKRSGAAEALEVLLPYSKDPFSVPDNVYLIGTMNTADRSLAGLDIALRRRFDFVEMAPRPDKLTGVKVAEIDISA
VLETMNRRIEVLLDREHRLGHAYFMSLENSNDLSRLSNIFRQQIIPLLQEYFFEDWQRIAWILNDHRKDERFRFIVPAPY
SVDSLFGDTGQVAQDSKAWRMNEEAFHKPQSYLGIVGDGKV

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=184, Percent_Identity=40.2173913043478, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 94759; Mature: 94628

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSTDDTLWREFLQVWPLERLREMTLAEYTTAGDKDCFVYWLEFRLVDYGSIAGGSAFK
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCCCCCEEE
FAIFSRKETGAREGDTSLAYDDRYGWYRRFGTSPEEAFQTIRGHVVAVAEAARDGRLDEI
EEEEECCCCCCCCCCCCCEECCCCCHHHHCCCCHHHHHHHHHHHEEEEEHHHCCCCCCCC
DNSPLGAAYRWKIAFHYQPLRNPSTPCVYVRKPLLHFLGLSAADTTTPQSELYQALARLR
CCCCCCCEEEEEEEEEEECCCCCCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
QADESIFVFSERLWKDWVADTPFVVKLSEGAIKHGYLPVNLISAPFPETMYGGKTPNEAA
HCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCCCHHCCCCCCCHHH
DVAHFRTDTGLEFDTDLRVSSPGSARLRHRLGGYFEDIGAAAGDLITLTQDGDGTYLLSS
HHHHHCCCCCCCCCCCCEECCCCHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCCEEEEC
KLAVHAIGPTASAATITARASVKEAAAVRPPLNQILFGPPGTGKTYEAIEKALEILDPSF
CEEEEEECCCCCCEEEEEHHHHHHHHHCCCCHHHEEECCCCCCHHHHHHHHHHHHHCCHH
LLQLGDDESNERRRALKRRYDELAKEGLVQFVTFHQSFSYENFVEGLSASSDPDTKQVRY
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHEEE
EVEDGVFKELCESARTRLVRRSDDAPLELSGRRIWKLSLGDSITEGHIFEECMEKGLALM
EHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCCCHHHHHHHHCCCEEE
GFGNNADFSQCQSREDIQKMFAASGEPLSLGAYPITAIKTFVCQMKLGDLIVVSEGNLKF
ECCCCCCHHHHCCHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHEECCCEEEEECCCEEE
RAIGEITGDYQLLPRDSDTYSQARPVRWLRGYKPALPYGALMENRFSQMTVYELRAGSID
EEEECCCCCEEECCCCCCCHHHHCCHHHHHCCCCCCCHHHHHHHHHHHEEEEEECCCCCC
HDKFRELLKSECIATGADRPHVLIIDEINRGNVSRIFGELITLIEPSKRSGAAEALEVLL
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHC
PYSKDPFSVPDNVYLIGTMNTADRSLAGLDIALRRRFDFVEMAPRPDKLTGVKVAEIDIS
CCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEHHHH
AVLETMNRRIEVLLDREHRLGHAYFMSLENSNDLSRLSNIFRQQIIPLLQEYFFEDWQRI
HHHHHHHHHEEEEECCCHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWILNDHRKDERFRFIVPAPYSVDSLFGDTGQVAQDSKAWRMNEEAFHKPQSYLGIVGDG
HHHHHCCCCCCCEEEEECCCCCHHHHCCCCCCCCCCCCHHCCCHHHHHCCCHHEEEECCC
KV
CC
>Mature Secondary Structure 
AFSTDDTLWREFLQVWPLERLREMTLAEYTTAGDKDCFVYWLEFRLVDYGSIAGGSAFK
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCCCCCEEE
FAIFSRKETGAREGDTSLAYDDRYGWYRRFGTSPEEAFQTIRGHVVAVAEAARDGRLDEI
EEEEECCCCCCCCCCCCCEECCCCCHHHHCCCCHHHHHHHHHHHEEEEEHHHCCCCCCCC
DNSPLGAAYRWKIAFHYQPLRNPSTPCVYVRKPLLHFLGLSAADTTTPQSELYQALARLR
CCCCCCCEEEEEEEEEEECCCCCCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
QADESIFVFSERLWKDWVADTPFVVKLSEGAIKHGYLPVNLISAPFPETMYGGKTPNEAA
HCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCCCHHCCCCCCCHHH
DVAHFRTDTGLEFDTDLRVSSPGSARLRHRLGGYFEDIGAAAGDLITLTQDGDGTYLLSS
HHHHHCCCCCCCCCCCCEECCCCHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCCEEEEC
KLAVHAIGPTASAATITARASVKEAAAVRPPLNQILFGPPGTGKTYEAIEKALEILDPSF
CEEEEEECCCCCCEEEEEHHHHHHHHHCCCCHHHEEECCCCCCHHHHHHHHHHHHHCCHH
LLQLGDDESNERRRALKRRYDELAKEGLVQFVTFHQSFSYENFVEGLSASSDPDTKQVRY
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHEEE
EVEDGVFKELCESARTRLVRRSDDAPLELSGRRIWKLSLGDSITEGHIFEECMEKGLALM
EHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCCCHHHHHHHHCCCEEE
GFGNNADFSQCQSREDIQKMFAASGEPLSLGAYPITAIKTFVCQMKLGDLIVVSEGNLKF
ECCCCCCHHHHCCHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHEECCCEEEEECCCEEE
RAIGEITGDYQLLPRDSDTYSQARPVRWLRGYKPALPYGALMENRFSQMTVYELRAGSID
EEEECCCCCEEECCCCCCCHHHHCCHHHHHCCCCCCCHHHHHHHHHHHEEEEEECCCCCC
HDKFRELLKSECIATGADRPHVLIIDEINRGNVSRIFGELITLIEPSKRSGAAEALEVLL
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHC
PYSKDPFSVPDNVYLIGTMNTADRSLAGLDIALRRRFDFVEMAPRPDKLTGVKVAEIDIS
CCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEHHHH
AVLETMNRRIEVLLDREHRLGHAYFMSLENSNDLSRLSNIFRQQIIPLLQEYFFEDWQRI
HHHHHHHHHEEEEECCCHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWILNDHRKDERFRFIVPAPYSVDSLFGDTGQVAQDSKAWRMNEEAFHKPQSYLGIVGDG
HHHHHCCCCCCCEEEEECCCCCHHHHCCCCCCCCCCCCHHCCCHHHHHCCCHHEEEECCC
KV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]