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The map label for this gene is ispB [H]

Identifier: 66767704

GI number: 66767704

Start: 1663498

End: 1664496

Strand: Reverse

Name: ispB [H]

Synonym: XC_1377

Alternate gene names: 66767704

Gene position: 1664496-1663498 (Counterclockwise)

Preceding gene: 77761150

Following gene: 66767698

Centisome position: 32.33

GC content: 67.47

Gene sequence:

>999_bases
ATGACCATCGCCCAAGACCTCCCCACCGTACTGGGCCTGCCCCAGATCCAGTCCCTCGCCGCGCCCGACATGGCGGCGGT
CGATGCCTTGATCCGCCGCCGTCTGGCGTCGGACGTCGTGCTGATCAACCAGATTGCCGATCACATCATCTCCGCCGGCG
GCAAACGCCTGCGCCCGATGCTGGTGATGCTGGCCGGCCACGCCGCGGGCGGCTCCGGGCCGGAGCACCACCAGCTCGCG
GCGATCATCGAGTTCATCCATACCTCCACGCTGCTGCATGACGACGTGGTGGACGAATCAGACCTGCGCCGCGGCCGCAG
CACCGCCAATGCGCTATGGGGCAACGCGCCCAGCGTGCTGGTGGGCGATTTCCTGTATTCGCGCAGCTTCCAACTGATGG
TCGAACTGGACCGCATGGAAGTAATGCAGATCCTGGCCGACACCACCAACCGCATCGCCGAAGGCGAAGTGCTGCAGCTC
TTGCATGTACACAACCCCGACACCGATGAAGCCGCGTACCTGCGCGTGATCGAGCGCAAGACCGCGGTGCTGTTTGCCGC
CGGCACGCGCCTGGGCGCACTGGCCTCGGGCGCGGATGCGCAGGTGCAGCAGTGCCTGTACGACTACGGCATGCAGCTGG
GCTTTGCGTTCCAGATCGCCGACGACGTGCTCGACTACGCCGCAGATGCCACCGACCTGGGCAAGAACCTGGGCGACGAC
CTGGCCGAGGGCAAGGCCACCCTGCCGCTGATCCACGCCATTGCGCATTCCGATGCCGCCACCCAGCAGCGCCTGCGGCA
GATCGTCGAACAGGGCGATGCCGAGGCGATGCCGGAGGTGTTGACGGCCATCCGCGCAACCGGCGGGCTCGATTACAGCC
GCCAGCGCGCTGCCGAATATGCCGCCGCCGCCGAACAGGCCCTGGACGGCTTGCCGCCGAGCCCGGCAGTGGCCGCATTA
CGCGGCCTGGCGCGTTATGCGGTGGAACGGACGCACTGA

Upstream 100 bases:

>100_bases
CAGCTGGTTCCGGGGCCACCGCGCAAGGGCTGGAACAGGCGCGGCGGGACGGATCTGGCCCGGGCACCTATAATCGGCCG
ACATCTCGATTTGCCTGCGC

Downstream 100 bases:

>100_bases
TCGCAACACGCGGCGCTGGGCGAGTGTCCGGCGCCGCCACCGAGCGAAGTGTCCCGCAAAGCGCTTGTGGTTGTCAGTCC
GGCACCGCCCTGCAGGGCCG

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTIAQDLPTVLGLPQIQSLAAPDMAAVDALIRRRLASDVVLINQIADHIISAGGKRLRPMLVMLAGHAAGGSGPEHHQLA
AIIEFIHTSTLLHDDVVDESDLRRGRSTANALWGNAPSVLVGDFLYSRSFQLMVELDRMEVMQILADTTNRIAEGEVLQL
LHVHNPDTDEAAYLRVIERKTAVLFAAGTRLGALASGADAQVQQCLYDYGMQLGFAFQIADDVLDYAADATDLGKNLGDD
LAEGKATLPLIHAIAHSDAATQQRLRQIVEQGDAEAMPEVLTAIRATGGLDYSRQRAAEYAAAAEQALDGLPPSPAVAAL
RGLARYAVERTH

Sequences:

>Translated_332_residues
MTIAQDLPTVLGLPQIQSLAAPDMAAVDALIRRRLASDVVLINQIADHIISAGGKRLRPMLVMLAGHAAGGSGPEHHQLA
AIIEFIHTSTLLHDDVVDESDLRRGRSTANALWGNAPSVLVGDFLYSRSFQLMVELDRMEVMQILADTTNRIAEGEVLQL
LHVHNPDTDEAAYLRVIERKTAVLFAAGTRLGALASGADAQVQQCLYDYGMQLGFAFQIADDVLDYAADATDLGKNLGDD
LAEGKATLPLIHAIAHSDAATQQRLRQIVEQGDAEAMPEVLTAIRATGGLDYSRQRAAEYAAAAEQALDGLPPSPAVAAL
RGLARYAVERTH
>Mature_331_residues
TIAQDLPTVLGLPQIQSLAAPDMAAVDALIRRRLASDVVLINQIADHIISAGGKRLRPMLVMLAGHAAGGSGPEHHQLAA
IIEFIHTSTLLHDDVVDESDLRRGRSTANALWGNAPSVLVGDFLYSRSFQLMVELDRMEVMQILADTTNRIAEGEVLQLL
HVHNPDTDEAAYLRVIERKTAVLFAAGTRLGALASGADAQVQQCLYDYGMQLGFAFQIADDVLDYAADATDLGKNLGDDL
AEGKATLPLIHAIAHSDAATQQRLRQIVEQGDAEAMPEVLTAIRATGGLDYSRQRAAEYAAAAEQALDGLPPSPAVAALR
GLARYAVERTH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=320, Percent_Identity=29.0625, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4758430, Length=277, Percent_Identity=26.3537906137184, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI83700220, Length=277, Percent_Identity=26.3537906137184, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=52.3364485981308, Blast_Score=326, Evalue=1e-90,
Organism=Escherichia coli, GI1786623, Length=241, Percent_Identity=33.195020746888, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17505681, Length=301, Percent_Identity=31.5614617940199, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319475, Length=302, Percent_Identity=33.112582781457, Blast_Score=142, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6325188, Length=249, Percent_Identity=24.8995983935743, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24651612, Length=297, Percent_Identity=30.976430976431, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI281365769, Length=285, Percent_Identity=25.9649122807018, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24660002, Length=285, Percent_Identity=25.9649122807018, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35579; Mature: 35448

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAQDLPTVLGLPQIQSLAAPDMAAVDALIRRRLASDVVLINQIADHIISAGGKRLRPM
CCCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LVMLAGHAAGGSGPEHHQLAAIIEFIHTSTLLHDDVVDESDLRRGRSTANALWGNAPSVL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHCCCCCHHH
VGDFLYSRSFQLMVELDRMEVMQILADTTNRIAEGEVLQLLHVHNPDTDEAAYLRVIERK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHH
TAVLFAAGTRLGALASGADAQVQQCLYDYGMQLGFAFQIADDVLDYAADATDLGKNLGDD
HHHEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHH
LAEGKATLPLIHAIAHSDAATQQRLRQIVEQGDAEAMPEVLTAIRATGGLDYSRQRAAEY
HHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
AAAAEQALDGLPPSPAVAALRGLARYAVERTH
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIAQDLPTVLGLPQIQSLAAPDMAAVDALIRRRLASDVVLINQIADHIISAGGKRLRPM
CCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LVMLAGHAAGGSGPEHHQLAAIIEFIHTSTLLHDDVVDESDLRRGRSTANALWGNAPSVL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHCCCCCHHH
VGDFLYSRSFQLMVELDRMEVMQILADTTNRIAEGEVLQLLHVHNPDTDEAAYLRVIERK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHH
TAVLFAAGTRLGALASGADAQVQQCLYDYGMQLGFAFQIADDVLDYAADATDLGKNLGDD
HHHEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHH
LAEGKATLPLIHAIAHSDAATQQRLRQIVEQGDAEAMPEVLTAIRATGGLDYSRQRAAEY
HHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
AAAAEQALDGLPPSPAVAALRGLARYAVERTH
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]