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The map label for this gene is rsmE [H]

Identifier: 66767551

GI number: 66767551

Start: 1498641

End: 1499375

Strand: Reverse

Name: rsmE [H]

Synonym: XC_1224

Alternate gene names: 66767551

Gene position: 1499375-1498641 (Counterclockwise)

Preceding gene: 66767552

Following gene: 66767549

Centisome position: 29.12

GC content: 67.48

Gene sequence:

>735_bases
ATGCGCCTGACCCGTTCGTATGTTGCATTGCCGCTGCACAGCGGCGGCGATGTCACGCTGCCGGAGGAATCGGCCAACCA
CCTGCTGCGTGTGCTGCGGCTGCGCGAAGGCGATGCCTGCGTGCTGTTCAATGGCGACGGCAGTGACTACCACGCACGCA
TCACCGTGGCCGGCAAGCGCGAGGCGCGGGCTGTGGTCGAGCGCGTCGAGGCAGTGAGCAACGAACCGCCGCTGGCAATC
ACCCTGCTGCAGGGCATTGCGCGCGGCGAGAAGATGGACCTGATTCTGCAGAAAGCCACCGAGCTCGGCGTGACAGCGGT
CGTGCCGGTCAATGCCGAGCGCACCGAGGTGAAGTTGGATGCCGCGCGCCTGGAAAAACGCATGGCGCATTGGCGCAGCG
TGATTGTCTCGGCCTGCGAGCAATCCGGGCGCGCACGCATTCCCTCGCTGGCACCGCCTTCCTCGCTGCAGGACGCCGCG
CGTGCAAGCGATGCACAGGCGCTGCGGTTGACGCTGGACCCGCACGGCGAACATCGCCTGGGCACGCTGGCGGTGCAGCC
CGGGCAAGAGCTGATTGTGGCGATTGGCCCGGAAGGCGGCTGGTCACCGCGCGACCGCACAACGCTGGGCGAGGCCGGCT
TTACCGGCTTGCGCCTGGGTCCCCGCATCCTGCGCACCGAAACCGCGGGATTGGCAGCGATTGCGGCGCTGCAGTCGCGA
TTTGGGGATTTGTAG

Upstream 100 bases:

>100_bases
TGGGCGACGTGCTGTACTGGATGCCGCCGTATTGCGTGGACGACGCACAGCTTGCGCTGCTTGCCGACACCACCCGCCAC
GCCATCGCCAAGGCCGTTGC

Downstream 100 bases:

>100_bases
GGCCGGTTGTTCGTGTGTGTGCGGCACTGGCCAGGCACGCCCTGTCAGGGCTACGTTTCTGTAGGAGCGCGCTCGGGCGC
GAGGGCGTTATCGGTAGAGC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRLTRSYVALPLHSGGDVTLPEESANHLLRVLRLREGDACVLFNGDGSDYHARITVAGKREARAVVERVEAVSNEPPLAI
TLLQGIARGEKMDLILQKATELGVTAVVPVNAERTEVKLDAARLEKRMAHWRSVIVSACEQSGRARIPSLAPPSSLQDAA
RASDAQALRLTLDPHGEHRLGTLAVQPGQELIVAIGPEGGWSPRDRTTLGEAGFTGLRLGPRILRTETAGLAAIAALQSR
FGDL

Sequences:

>Translated_244_residues
MRLTRSYVALPLHSGGDVTLPEESANHLLRVLRLREGDACVLFNGDGSDYHARITVAGKREARAVVERVEAVSNEPPLAI
TLLQGIARGEKMDLILQKATELGVTAVVPVNAERTEVKLDAARLEKRMAHWRSVIVSACEQSGRARIPSLAPPSSLQDAA
RASDAQALRLTLDPHGEHRLGTLAVQPGQELIVAIGPEGGWSPRDRTTLGEAGFTGLRLGPRILRTETAGLAAIAALQSR
FGDL
>Mature_244_residues
MRLTRSYVALPLHSGGDVTLPEESANHLLRVLRLREGDACVLFNGDGSDYHARITVAGKREARAVVERVEAVSNEPPLAI
TLLQGIARGEKMDLILQKATELGVTAVVPVNAERTEVKLDAARLEKRMAHWRSVIVSACEQSGRARIPSLAPPSSLQDAA
RASDAQALRLTLDPHGEHRLGTLAVQPGQELIVAIGPEGGWSPRDRTTLGEAGFTGLRLGPRILRTETAGLAAIAALQSR
FGDL

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=244, Percent_Identity=42.2131147540984, Blast_Score=185, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26162; Mature: 26162

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTRSYVALPLHSGGDVTLPEESANHLLRVLRLREGDACVLFNGDGSDYHARITVAGKR
CCCCCCEEEEEECCCCCEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEECCH
EARAVVERVEAVSNEPPLAITLLQGIARGEKMDLILQKATELGVTAVVPVNAERTEVKLD
HHHHHHHHHHHHCCCCCEEHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEHH
AARLEKRMAHWRSVIVSACEQSGRARIPSLAPPSSLQDAARASDAQALRLTLDPHGEHRL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCE
GTLAVQPGQELIVAIGPEGGWSPRDRTTLGEAGFTGLRLGPRILRTETAGLAAIAALQSR
EEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHH
FGDL
HCCC
>Mature Secondary Structure
MRLTRSYVALPLHSGGDVTLPEESANHLLRVLRLREGDACVLFNGDGSDYHARITVAGKR
CCCCCCEEEEEECCCCCEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEECCH
EARAVVERVEAVSNEPPLAITLLQGIARGEKMDLILQKATELGVTAVVPVNAERTEVKLD
HHHHHHHHHHHHCCCCCEEHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEHH
AARLEKRMAHWRSVIVSACEQSGRARIPSLAPPSSLQDAARASDAQALRLTLDPHGEHRL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCE
GTLAVQPGQELIVAIGPEGGWSPRDRTTLGEAGFTGLRLGPRILRTETAGLAAIAALQSR
EEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHH
FGDL
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]