Click here to switch to the map view.

The map label for this gene is gshB [H]

Identifier: 66767510

GI number: 66767510

Start: 1433234

End: 1434184

Strand: Reverse

Name: gshB [H]

Synonym: XC_1182

Alternate gene names: 66767510

Gene position: 1434184-1433234 (Counterclockwise)

Preceding gene: 66767519

Following gene: 66767509

Centisome position: 27.86

GC content: 68.35

Gene sequence:

>951_bases
ATGTCGCTCGACGTCGTTGTGGTGATGGATCCCATCGCCTCCATCAAGATCGCCAAAGACACCACCTTCGCCATGTTGCT
GGAAGCCCAGCGCCGTGGTCACCGCCTGCATTATGTGCGCCCCGGCGGACTCAGCCTGCATGAAGGACGCGCCGTGGCGC
AGGTCGCGCCGCTGAGCGTGCGCGAGGACAAGGCCAGCTGGTTCACCCTGGGCGCGTTCACCGAGCTGGTGTTTGGTCCC
GGCCAGGTGGTGCTGATGCGCAAGGACCCGCCGGTGGATGCCGAGTTCATCTACGACACCCAGGTGCTGGCCGTGGCGCA
GCGCGCCGGTGCCCAGGTGGTCAACGACCCGCAGGGCCTGCGCGACTACAACGAGAAGCTGGCCGCGCTGCTGTTCCCGC
AGTGCTGCCCGCCGACCCTGGTCAGCCGCGACGCGGCCGCGCTGAAGGCGTTCGTGCTCGCACATGGCCAGGCGGTGCTC
AAGCCGCTGGACGGCATGGGCGGGCGCTCGATCTTCCGCAGCGGCACCGGCGACCCCAATCTCAACGTGATCCTGGAAAC
GCTGACCGATGGCGGCCGCAAGCTGACCCTGGCGCAGCGCTTCATCCCCGACATCACTGCCGGCGACAAGCGCATCCTGC
TGGTGGACGGCGAGCCGGTGGACTACTGCCTGGCACGCATCCCGCAGGGCGACGAGTTCCGCGGCAACCTGGCCGCCGGC
GGCCGCGGCGAAGGCCGCCCGCTGTCCGAGCGCGACCGTTGGATCGCCGCGCAGGTGGGCCCGGAAATGAAACGCCGCGG
CATGCGCTTCGTGGGCCTGGACGTGATCGGCGATTACCTCACCGAAGTCAACGTCACCAGCCCCACCTGCGTGCGTGAGC
TGGATGCGCAGTTCGGGCTCAATATTGCCGGGCTGCTGTTCGATGCGATCGAGGCCGGCACCGCGCAATGA

Upstream 100 bases:

>100_bases
CGCCCTTGGACCGGGAAGTCGCGCATTCGCAACCCGCCGGGCGCGCCATCGCCCACCGCTTGAAGTTACCATCGGCAGCT
TGTTCTAAAGGTTTAGCCCC

Downstream 100 bases:

>100_bases
GCACGGCCGCGGCCTTGCCTGCACCGATGGACGAACGCCAGCGTCTGACCGCGACGCTGGTGATCTCGCTGCTGCTGCAT
GGCCTGCTGATCCTGGGTGT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSLDVVVVMDPIASIKIAKDTTFAMLLEAQRRGHRLHYVRPGGLSLHEGRAVAQVAPLSVREDKASWFTLGAFTELVFGP
GQVVLMRKDPPVDAEFIYDTQVLAVAQRAGAQVVNDPQGLRDYNEKLAALLFPQCCPPTLVSRDAAALKAFVLAHGQAVL
KPLDGMGGRSIFRSGTGDPNLNVILETLTDGGRKLTLAQRFIPDITAGDKRILLVDGEPVDYCLARIPQGDEFRGNLAAG
GRGEGRPLSERDRWIAAQVGPEMKRRGMRFVGLDVIGDYLTEVNVTSPTCVRELDAQFGLNIAGLLFDAIEAGTAQ

Sequences:

>Translated_316_residues
MSLDVVVVMDPIASIKIAKDTTFAMLLEAQRRGHRLHYVRPGGLSLHEGRAVAQVAPLSVREDKASWFTLGAFTELVFGP
GQVVLMRKDPPVDAEFIYDTQVLAVAQRAGAQVVNDPQGLRDYNEKLAALLFPQCCPPTLVSRDAAALKAFVLAHGQAVL
KPLDGMGGRSIFRSGTGDPNLNVILETLTDGGRKLTLAQRFIPDITAGDKRILLVDGEPVDYCLARIPQGDEFRGNLAAG
GRGEGRPLSERDRWIAAQVGPEMKRRGMRFVGLDVIGDYLTEVNVTSPTCVRELDAQFGLNIAGLLFDAIEAGTAQ
>Mature_315_residues
SLDVVVVMDPIASIKIAKDTTFAMLLEAQRRGHRLHYVRPGGLSLHEGRAVAQVAPLSVREDKASWFTLGAFTELVFGPG
QVVLMRKDPPVDAEFIYDTQVLAVAQRAGAQVVNDPQGLRDYNEKLAALLFPQCCPPTLVSRDAAALKAFVLAHGQAVLK
PLDGMGGRSIFRSGTGDPNLNVILETLTDGGRKLTLAQRFIPDITAGDKRILLVDGEPVDYCLARIPQGDEFRGNLAAGG
RGEGRPLSERDRWIAAQVGPEMKRRGMRFVGLDVIGDYLTEVNVTSPTCVRELDAQFGLNIAGLLFDAIEAGTAQ

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=310, Percent_Identity=57.741935483871, Blast_Score=363, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34174; Mature: 34043

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDVVVVMDPIASIKIAKDTTFAMLLEAQRRGHRLHYVRPGGLSLHEGRAVAQVAPLSV
CCEEEEEEECCCCEEEEECCCHHHHHHHHHHCCCEEEEECCCCCEECCCCEEEEECCCCE
REDKASWFTLGAFTELVFGPGQVVLMRKDPPVDAEFIYDTQVLAVAQRAGAQVVNDPQGL
ECCCCCEEEHHHHHHHHCCCCEEEEEECCCCCCCEEEECHHHHHHHHHCCCCCCCCCHHH
RDYNEKLAALLFPQCCPPTLVSRDAAALKAFVLAHGQAVLKPLDGMGGRSIFRSGTGDPN
HHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHCCHHHHHCCCCCCCCHHHCCCCCCCC
LNVILETLTDGGRKLTLAQRFIPDITAGDKRILLVDGEPVDYCLARIPQGDEFRGNLAAG
CEEEEEECCCCCCEEEHHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCC
GRGEGRPLSERDRWIAAQVGPEMKRRGMRFVGLDVIGDYLTEVNVTSPTCVRELDAQFGL
CCCCCCCCCHHHCEEEECCCHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHCC
NIAGLLFDAIEAGTAQ
CHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLDVVVVMDPIASIKIAKDTTFAMLLEAQRRGHRLHYVRPGGLSLHEGRAVAQVAPLSV
CEEEEEEECCCCEEEEECCCHHHHHHHHHHCCCEEEEECCCCCEECCCCEEEEECCCCE
REDKASWFTLGAFTELVFGPGQVVLMRKDPPVDAEFIYDTQVLAVAQRAGAQVVNDPQGL
ECCCCCEEEHHHHHHHHCCCCEEEEEECCCCCCCEEEECHHHHHHHHHCCCCCCCCCHHH
RDYNEKLAALLFPQCCPPTLVSRDAAALKAFVLAHGQAVLKPLDGMGGRSIFRSGTGDPN
HHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHCCHHHHHCCCCCCCCHHHCCCCCCCC
LNVILETLTDGGRKLTLAQRFIPDITAGDKRILLVDGEPVDYCLARIPQGDEFRGNLAAG
CEEEEEECCCCCCEEEHHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCC
GRGEGRPLSERDRWIAAQVGPEMKRRGMRFVGLDVIGDYLTEVNVTSPTCVRELDAQFGL
CCCCCCCCCHHHCEEEECCCHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHCC
NIAGLLFDAIEAGTAQ
CHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]