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The map label for this gene is otsA [H]

Identifier: 66767404

GI number: 66767404

Start: 1296518

End: 1297885

Strand: Reverse

Name: otsA [H]

Synonym: XC_1076

Alternate gene names: 66767404

Gene position: 1297885-1296518 (Counterclockwise)

Preceding gene: 66767405

Following gene: 66767403

Centisome position: 25.21

GC content: 62.72

Gene sequence:

>1368_bases
ATGAGTCGTCTGGTGGTAGTATCCAATCGTGTGGCCGTGCCTGGTGAAAGCCGTGCCGGCGGCCTGGCAGTGGGCTTGTT
GGCGGCGTTGAAGGAGCGCGGCGGGGTGTGGTTCGGCTGGAGCGGCAAGACCGTGCGCGGCGACAGCGGCGGCATGCACG
AGCAGACCGACGGTGACATCAAGTTCGTCACCATGGACCTCAACAAGCGCGACATCGATTCCTACTACAACGGCTTTGCC
AACCGCACGCTGTGGCCGCTGCTGCACTTCCGCCTGGACCTGGTCGATTACGACCGCGCCACGCGCGAAGGCTACCTGCG
CGTCAATCGCCTGTTCGCCGAGAAGCTGGCGCCCTTGCTCAAGGACAGCGACACGCTGTGGATCCACGATTACCACATGA
TTCCGCTCGGCGCGATGCTGCGCGAGCTCGGTGTGGGCTGCAAGATGGGCTTCTTCCTGCATGTGCCGATGCCGTCGGCC
GACCTGATGCAGGCGATGCCGGATCACGCGCGCTTGTTCAGCACGTTCTACGCGTACGACCTGGTTGGCTTCCAGACCCA
GCGCGATGCCGAACGCTTCAAGGCGTATGTGCGCCTGTTCGGTGGTGGGCGCGTGCTGGAGGGCGATCTGGTGGAAGGCC
CGGGTGGGCGCCGCTTCAGCGCTGCTGCGTTCCCGATCGGCATCGACACCGATCTGATTGCCAATCAGGCCAAGGCGTCG
GTCTCCAAGCAGGCAGTGCGCGATCTTCGCGAAAGCCTGCGCGGGCGCCAGCTCGCGATCGGCGTGGATCGCCTGGATTA
TTCCAAGGGTCTGCCCGAGCGCTTCCAGGGCTTTGAGCGCTATCTGGAGCGCTACCCGGATCAATCCGGCAGCCTCACCT
ATCTGCAGATTGCACCGGTCTCGCGCGGCGACGTCAACGAATATCGCCAGCTGCGCGGGCAGCTTGAGCAGATCGCAGGC
CACATCAACGGTGGTCATGCGGAGCCGGATTGGACGCCGCTGCGTTACGTCAACCAGAACTTCAGCCATGCCACGTTGAC
CGGATTTTATCGCGCTGCGGCCGTGGGTTTGGTGACACCATTGCGCGACGGCATGAATCTGGTCGCCAAGGAATTTGTGG
CAGCGCAGGATCCGGAGAATCCGGGTGTGCTGGTGTTGTCGTTGCTGGCCGGTGCGGCCGATGAGATGAAAGAGGCGCTG
TTGGTCAATCCGCATGATCTGGATGGTGTGGCCGATGCAATTGCGACGGCGGCGAGCATGCCGTTGGCAACGCGGGTGGA
GCGTTGGCACGCGATGATGGATCACTTGCGCAAGAACAACATCAATCATTGGCGGCAGCGGTATTTGCAGGCGTTGGCCG
ATATCTGA

Upstream 100 bases:

>100_bases
TTGAAAACGGCGAAGCCTGGGGCAATTTCCCCCAGACCTATTCGCATGTGGGCTTGATCATTGCAGCAATGCGCTTGTCG
CGGAGTTGGCAGGAGGCGTC

Downstream 100 bases:

>100_bases
GTCTGGCTGTTTCATGCGCGGTGTCGCGTTTCGCGTCACTGCGTTGATGCAATTTTCTCGCCGTGAGCATGAGAAAGCGT
TGCCTAGATGACTGCGCGCA

Product: trehalose-6-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSRLVVVSNRVAVPGESRAGGLAVGLLAALKERGGVWFGWSGKTVRGDSGGMHEQTDGDIKFVTMDLNKRDIDSYYNGFA
NRTLWPLLHFRLDLVDYDRATREGYLRVNRLFAEKLAPLLKDSDTLWIHDYHMIPLGAMLRELGVGCKMGFFLHVPMPSA
DLMQAMPDHARLFSTFYAYDLVGFQTQRDAERFKAYVRLFGGGRVLEGDLVEGPGGRRFSAAAFPIGIDTDLIANQAKAS
VSKQAVRDLRESLRGRQLAIGVDRLDYSKGLPERFQGFERYLERYPDQSGSLTYLQIAPVSRGDVNEYRQLRGQLEQIAG
HINGGHAEPDWTPLRYVNQNFSHATLTGFYRAAAVGLVTPLRDGMNLVAKEFVAAQDPENPGVLVLSLLAGAADEMKEAL
LVNPHDLDGVADAIATAASMPLATRVERWHAMMDHLRKNNINHWRQRYLQALADI

Sequences:

>Translated_455_residues
MSRLVVVSNRVAVPGESRAGGLAVGLLAALKERGGVWFGWSGKTVRGDSGGMHEQTDGDIKFVTMDLNKRDIDSYYNGFA
NRTLWPLLHFRLDLVDYDRATREGYLRVNRLFAEKLAPLLKDSDTLWIHDYHMIPLGAMLRELGVGCKMGFFLHVPMPSA
DLMQAMPDHARLFSTFYAYDLVGFQTQRDAERFKAYVRLFGGGRVLEGDLVEGPGGRRFSAAAFPIGIDTDLIANQAKAS
VSKQAVRDLRESLRGRQLAIGVDRLDYSKGLPERFQGFERYLERYPDQSGSLTYLQIAPVSRGDVNEYRQLRGQLEQIAG
HINGGHAEPDWTPLRYVNQNFSHATLTGFYRAAAVGLVTPLRDGMNLVAKEFVAAQDPENPGVLVLSLLAGAADEMKEAL
LVNPHDLDGVADAIATAASMPLATRVERWHAMMDHLRKNNINHWRQRYLQALADI
>Mature_454_residues
SRLVVVSNRVAVPGESRAGGLAVGLLAALKERGGVWFGWSGKTVRGDSGGMHEQTDGDIKFVTMDLNKRDIDSYYNGFAN
RTLWPLLHFRLDLVDYDRATREGYLRVNRLFAEKLAPLLKDSDTLWIHDYHMIPLGAMLRELGVGCKMGFFLHVPMPSAD
LMQAMPDHARLFSTFYAYDLVGFQTQRDAERFKAYVRLFGGGRVLEGDLVEGPGGRRFSAAAFPIGIDTDLIANQAKASV
SKQAVRDLRESLRGRQLAIGVDRLDYSKGLPERFQGFERYLERYPDQSGSLTYLQIAPVSRGDVNEYRQLRGQLEQIAGH
INGGHAEPDWTPLRYVNQNFSHATLTGFYRAAAVGLVTPLRDGMNLVAKEFVAAQDPENPGVLVLSLLAGAADEMKEALL
VNPHDLDGVADAIATAASMPLATRVERWHAMMDHLRKNNINHWRQRYLQALADI

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=458, Percent_Identity=49.3449781659389, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71987012, Length=435, Percent_Identity=29.6551724137931, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI115533560, Length=357, Percent_Identity=32.7731092436975, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI115533558, Length=357, Percent_Identity=32.7731092436975, Blast_Score=174, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6319602, Length=473, Percent_Identity=31.7124735729387, Blast_Score=268, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6323537, Length=387, Percent_Identity=27.1317829457364, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323917, Length=387, Percent_Identity=25.8397932816537, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6320279, Length=407, Percent_Identity=22.6044226044226, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI19920676, Length=486, Percent_Identity=35.1851851851852, Blast_Score=286, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 50717; Mature: 50585

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVVVSNRVAVPGESRAGGLAVGLLAALKERGGVWFGWSGKTVRGDSGGMHEQTDGDI
CCCEEEEECEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCEEECCCCCCCCCCCCCE
KFVTMDLNKRDIDSYYNGFANRTLWPLLHFRLDLVDYDRATREGYLRVNRLFAEKLAPLL
EEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KDSDTLWIHDYHMIPLGAMLRELGVGCKMGFFLHVPMPSADLMQAMPDHARLFSTFYAYD
CCCCEEEEEEEECCCHHHHHHHHCCCCEECEEEECCCCCHHHHHHCCHHHHHHHHHHHHH
LVGFQTQRDAERFKAYVRLFGGGRVLEGDLVEGPGGRRFSAAAFPIGIDTDLIANQAKAS
HHCCCCHHHHHHHHHHHHHHCCCEEEECCCEECCCCCEEECEECCCCCCHHHHHHHHHHH
VSKQAVRDLRESLRGRQLAIGVDRLDYSKGLPERFQGFERYLERYPDQSGSLTYLQIAPV
HHHHHHHHHHHHHCCCEEEEEECHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECC
SRGDVNEYRQLRGQLEQIAGHINGGHAEPDWTPLRYVNQNFSHATLTGFYRAAAVGLVTP
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LRDGMNLVAKEFVAAQDPENPGVLVLSLLAGAADEMKEALLVNPHDLDGVADAIATAASM
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCC
PLATRVERWHAMMDHLRKNNINHWRQRYLQALADI
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLVVVSNRVAVPGESRAGGLAVGLLAALKERGGVWFGWSGKTVRGDSGGMHEQTDGDI
CCEEEEECEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCEEECCCCCCCCCCCCCE
KFVTMDLNKRDIDSYYNGFANRTLWPLLHFRLDLVDYDRATREGYLRVNRLFAEKLAPLL
EEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KDSDTLWIHDYHMIPLGAMLRELGVGCKMGFFLHVPMPSADLMQAMPDHARLFSTFYAYD
CCCCEEEEEEEECCCHHHHHHHHCCCCEECEEEECCCCCHHHHHHCCHHHHHHHHHHHHH
LVGFQTQRDAERFKAYVRLFGGGRVLEGDLVEGPGGRRFSAAAFPIGIDTDLIANQAKAS
HHCCCCHHHHHHHHHHHHHHCCCEEEECCCEECCCCCEEECEECCCCCCHHHHHHHHHHH
VSKQAVRDLRESLRGRQLAIGVDRLDYSKGLPERFQGFERYLERYPDQSGSLTYLQIAPV
HHHHHHHHHHHHHCCCEEEEEECHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECC
SRGDVNEYRQLRGQLEQIAGHINGGHAEPDWTPLRYVNQNFSHATLTGFYRAAAVGLVTP
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LRDGMNLVAKEFVAAQDPENPGVLVLSLLAGAADEMKEALLVNPHDLDGVADAIATAASM
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCC
PLATRVERWHAMMDHLRKNNINHWRQRYLQALADI
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA