Click here to switch to the map view.

The map label for this gene is pfkA1 [H]

Identifier: 66767204

GI number: 66767204

Start: 1050470

End: 1051726

Strand: Reverse

Name: pfkA1 [H]

Synonym: XC_0872

Alternate gene names: 66767204

Gene position: 1051726-1050470 (Counterclockwise)

Preceding gene: 66767209

Following gene: 66767203

Centisome position: 20.43

GC content: 67.3

Gene sequence:

>1257_bases
ATGACCAACGGCAACCTGTTGTATGCCCAGTCCGGCGGTGTCACTGCCGTCATCAACGCCACCGCTGCCGGGGTGATCGG
CGAAGCGCGCGCACGCAAGATCAAGGTGCTGGCCGCACGCAACGGCATCCTGGGCGCACTGCGCGAGGAATTGATCGACA
CCTCCAAGGAATCGGCCGCGGCGATCGCCGCGCTGGCGCAGACCCCCGGCGGCGCGTTCGGCTCCTGCCGCTACAAGCTC
AAATCGCTGGAGCAGGACCGCGCCAAATACGAGCGCCTGCTGGAGGTGCTGCGCGCGCACGACGTGCGCTGGTTCCTCTA
CAACGGCGGCAACGACTCGGCCGATACCGCACTGAAGGTCTCGCAGCTGGCCAAGGCGTTCGGCTACCCGCTGCACTGCA
TCGGCGTGCCCAAGACCATCGACAACGACCTGGCGGTGACCGACACCTGCCCCGGCTTCGGCTCGGCCGCCAAGTACACC
GCGGTCTCGGTGCGCGAAGCGGCGCTGGACGTGGCCGCCATGGCCGACACCTCCACCAAGGTCTTCATCTACGAAGCGAT
GGGCCGCCACGCCGGCTGGCTGGCCGCCGCCGCAGGCCTGGCCGGCCAAGGCCCGGACGACGCGCCGCAGATCATCCTGC
TGCCCGAGCGCGCCTTTGATCAGCCCGCGTTCCTGGCCAAGGTGCGCCAGGTGGTGGAACGGGTCGGCTGGTGCGTGGTG
GTGGCCAGCGAGGGCATCCAGGACGCACAGGGCAAATTCGTCGCCGATGCCGGTGGCGCGACCGACTCGTTCGGCCACGC
GCAACTGGGCGGTGTGGCCTCGTTCCTCGCAGCACAGGTCAAACAGGAACTCGGCTACAAGGTGCACTGGACGCTGCCCG
ATTACCTACAGCGTTCCGCACGCCACCTCGCCTCCAAGACCGACTGGGAACAAGCCCAGGCTGTGGGCAAGGCCGCCGTG
CAGTACGCACTCAAGGGCATGAACGCGGTGATCCCGGTGATCGAGCGCGTCAGCGACGCCCCCTATCGCTGGAAGATCGT
GCCCGCCCCGCTGCACAAGGTGGCCAACCACGAAAAGAAGATGCCACCTAGCTTCCTGCGCAAGGACGGCTTCGGCCTCA
CCGAACGCGCCCGCCGCTACTTCGCCCCACTGATCAAGGGCGAGGCCCCGCTGGCCTACGGCAGCGATGGCCTGCCGAAG
TATGTGACGTTGAAGAATGTGGCGGTGGCGAAGAAATTGCCGGCTTGGGAGGGCTGA

Upstream 100 bases:

>100_bases
TCGCCAGCGCTGACCGTATGGTCGTGCAGCTTACCGCATCCGGGGAATGTCCCCGCAATGTCCCCCGCGCCATGTCGCCA
TCTGAGGAGTGTGTCCCCCT

Downstream 100 bases:

>100_bases
ACAGGGGGAGGACGGCCGCCAGCCTGTGCAATTGTTACAGCGCCGGCGCTCGGCTTGAGCTGCGCAGGGATGCAGCCAAT
TCCGGCTACCCAGGCCAGGG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: ATP-PFK; Phosphofructokinase 1; Phosphohexokinase 1 [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MTNGNLLYAQSGGVTAVINATAAGVIGEARARKIKVLAARNGILGALREELIDTSKESAAAIAALAQTPGGAFGSCRYKL
KSLEQDRAKYERLLEVLRAHDVRWFLYNGGNDSADTALKVSQLAKAFGYPLHCIGVPKTIDNDLAVTDTCPGFGSAAKYT
AVSVREAALDVAAMADTSTKVFIYEAMGRHAGWLAAAAGLAGQGPDDAPQIILLPERAFDQPAFLAKVRQVVERVGWCVV
VASEGIQDAQGKFVADAGGATDSFGHAQLGGVASFLAAQVKQELGYKVHWTLPDYLQRSARHLASKTDWEQAQAVGKAAV
QYALKGMNAVIPVIERVSDAPYRWKIVPAPLHKVANHEKKMPPSFLRKDGFGLTERARRYFAPLIKGEAPLAYGSDGLPK
YVTLKNVAVAKKLPAWEG

Sequences:

>Translated_418_residues
MTNGNLLYAQSGGVTAVINATAAGVIGEARARKIKVLAARNGILGALREELIDTSKESAAAIAALAQTPGGAFGSCRYKL
KSLEQDRAKYERLLEVLRAHDVRWFLYNGGNDSADTALKVSQLAKAFGYPLHCIGVPKTIDNDLAVTDTCPGFGSAAKYT
AVSVREAALDVAAMADTSTKVFIYEAMGRHAGWLAAAAGLAGQGPDDAPQIILLPERAFDQPAFLAKVRQVVERVGWCVV
VASEGIQDAQGKFVADAGGATDSFGHAQLGGVASFLAAQVKQELGYKVHWTLPDYLQRSARHLASKTDWEQAQAVGKAAV
QYALKGMNAVIPVIERVSDAPYRWKIVPAPLHKVANHEKKMPPSFLRKDGFGLTERARRYFAPLIKGEAPLAYGSDGLPK
YVTLKNVAVAKKLPAWEG
>Mature_417_residues
TNGNLLYAQSGGVTAVINATAAGVIGEARARKIKVLAARNGILGALREELIDTSKESAAAIAALAQTPGGAFGSCRYKLK
SLEQDRAKYERLLEVLRAHDVRWFLYNGGNDSADTALKVSQLAKAFGYPLHCIGVPKTIDNDLAVTDTCPGFGSAAKYTA
VSVREAALDVAAMADTSTKVFIYEAMGRHAGWLAAAAGLAGQGPDDAPQIILLPERAFDQPAFLAKVRQVVERVGWCVVV
ASEGIQDAQGKFVADAGGATDSFGHAQLGGVASFLAAQVKQELGYKVHWTLPDYLQRSARHLASKTDWEQAQAVGKAAVQ
YALKGMNAVIPVIERVSDAPYRWKIVPAPLHKVANHEKKMPPSFLRKDGFGLTERARRYFAPLIKGEAPLAYGSDGLPKY
VTLKNVAVAKKLPAWEG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=223, Percent_Identity=28.6995515695067, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 44656; Mature: 44524

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNGNLLYAQSGGVTAVINATAAGVIGEARARKIKVLAARNGILGALREELIDTSKESAA
CCCCCEEEEECCCEEEEEECHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCHHHHH
AIAALAQTPGGAFGSCRYKLKSLEQDRAKYERLLEVLRAHDVRWFLYNGGNDSADTALKV
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
SQLAKAFGYPLHCIGVPKTIDNDLAVTDTCPGFGSAAKYTAVSVREAALDVAAMADTSTK
HHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
VFIYEAMGRHAGWLAAAAGLAGQGPDDAPQIILLPERAFDQPAFLAKVRQVVERVGWCVV
EEEEECCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEE
VASEGIQDAQGKFVADAGGATDSFGHAQLGGVASFLAAQVKQELGYKVHWTLPDYLQRSA
EECCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHH
RHLASKTDWEQAQAVGKAAVQYALKGMNAVIPVIERVSDAPYRWKIVPAPLHKVANHEKK
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCC
MPPSFLRKDGFGLTERARRYFAPLIKGEAPLAYGSDGLPKYVTLKNVAVAKKLPAWEG
CCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCCCC
>Mature Secondary Structure 
TNGNLLYAQSGGVTAVINATAAGVIGEARARKIKVLAARNGILGALREELIDTSKESAA
CCCCEEEEECCCEEEEEECHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCHHHHH
AIAALAQTPGGAFGSCRYKLKSLEQDRAKYERLLEVLRAHDVRWFLYNGGNDSADTALKV
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
SQLAKAFGYPLHCIGVPKTIDNDLAVTDTCPGFGSAAKYTAVSVREAALDVAAMADTSTK
HHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
VFIYEAMGRHAGWLAAAAGLAGQGPDDAPQIILLPERAFDQPAFLAKVRQVVERVGWCVV
EEEEECCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEE
VASEGIQDAQGKFVADAGGATDSFGHAQLGGVASFLAAQVKQELGYKVHWTLPDYLQRSA
EECCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHH
RHLASKTDWEQAQAVGKAAVQYALKGMNAVIPVIERVSDAPYRWKIVPAPLHKVANHEKK
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCC
MPPSFLRKDGFGLTERARRYFAPLIKGEAPLAYGSDGLPKYVTLKNVAVAKKLPAWEG
CCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9055413; 12000953 [H]