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The map label for this gene is leuA [H]

Identifier: 66767170

GI number: 66767170

Start: 1007240

End: 1008802

Strand: Reverse

Name: leuA [H]

Synonym: XC_0837

Alternate gene names: 66767170

Gene position: 1008802-1007240 (Counterclockwise)

Preceding gene: 66767171

Following gene: 66767165

Centisome position: 19.59

GC content: 65.2

Gene sequence:

>1563_bases
GTGAACACGACCGTATCCAACCAGACCCCGCATATCCGAATTTTCGACACCACCCTGCGCGACGGCGAGCAATCCCCCGG
CTGCAGCATGACGCCCCAACAAAAGCTGGTCATGGCGCGCGCGCTGGACGCGCTTGGCGTGGACATCATCGAAACCGGTT
TCCCGGCCAGCTCGTATTCCGACCGCGAAGCGGTGGCGATGATGGGCCGCGAATTGCGCCGCCCCACCCTGGCGGTGCTG
TCGCGCTGCCTGCAGGCCGATATCGAGATCTCTGCCCGTGCACTGGAAGCGGCCGCCAACCCGCGCCTGCATGTGTTCCT
GTCCACCAGCCCGCTGCACCGCGAGCACAAGCTGCGCATGAGCCGCGAGCAGGTGCTGGAATCGGTGCACAAGCACGTGA
CGCTGGCGCGCGGCTACATCGACGACATCGAGTTCTCGGCCGAAGATGCCACGCGCACCGAAGAAGATTTCCTGGCCGAA
GTGACACGCGTGGCCATCGCCGCAGGTGCGACCACGATCAACCTGCCGGACACGGTGGGTTTCACCACGCCGGAAGAAAT
CCGCGGCATGTTCTCGCGGCTGATCGCCAGCGTCGAAGGTGCCGAGAAGGTGATCTTCAGCACGCACTGCCACAACGACC
TGGGCCTGGCGGCTGCCAACTCGCTGGCCGCAATCGAAGGCGGCGCGCGCCAGGTGGAATGCACCATCAACGGCATCGGC
GAACGCGCCGGCAATTGCGCGCTGGAAGAAATCACCATGGCCCTGAAGGTGCGTGGTGCGTTCTACAACCTGGACACCGC
AATCAATACGCCGCGCATCGTCTCCACTTCGCAGCTGCTGCAGCGTTTGGTTGGCATGCCGGTGCAGCGCAACAAGGCGG
TGGTGGGCGGCAACGCGTTCGCGCATGAATCGGGCATCCACCAGCACGGCATGCTGCGTCACCGCGGCACTTACGAAATC
ATGCGCCCGGAAGATGTGGGCTGGGAGTCGTCGCAGATGGTGCTGGGCCGCCACAGCGGCCGCGCGGCAGTGGAACAGCG
CCTGCGCGCGCTGGGTTACCTGCTGGAGGAAGATGAGGCCAAGCTGGTGTTCGAACAGTTCAAGGCATTGTGCGAAAAGC
AGCGCGTCGTCACCGACGCCGACCTGCAAGCCTTGATGCAGGACGCCACCGTGCAGGAAGGCTACCGCCTGGCCTCGATG
ACCATCAGCGATGTCGGCAGCCGCGCCAATGCACTGGTTGAGCTGTCCGACCCGGACGGCAATCGCGTTGCAGAAACGGC
GCAAGGCAATGGCCCGGTAGACGCGCTATTCGGCGCGCTGGCGTCGGCCACCGGTGTGAAGCTGGAATTGGACAGCTACC
AGGTACACAGCGTAGGCATCGGCGCCGATGCCCGCGGCGAAGCCAGCCTGAGCGTGCGCCACGACGGCGTGGAATACGAA
GGCACCGGCACCAGCAAGGACATCATCGAGGCCAGTGCCCTGGCCTGGCTCGATGTTGCCAACCGCCTGCTGCGTCAGCG
CGAGCGTGGCGTGGTGGCGGGGAAGACGGCGGCGGTGGCGTAA

Upstream 100 bases:

>100_bases
AACGCAGCACCAAAGCATCACCGCCATCCATTGCTATTCCCCGCCCATCCGCAGTCGCATCAACGCCTGTCACCGCCATC
GCAGTAGAGGAGTCTTCCTG

Downstream 100 bases:

>100_bases
CGCGCGGTTTGGGTACAGCGTCGGCGACACGATGACGTTAGTAAAACGGCCAGCGAGTCATACGCTGGCCGTTTTGCGTT
TGTGCTTCCGCTAGTTTTTG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MNTTVSNQTPHIRIFDTTLRDGEQSPGCSMTPQQKLVMARALDALGVDIIETGFPASSYSDREAVAMMGRELRRPTLAVL
SRCLQADIEISARALEAAANPRLHVFLSTSPLHREHKLRMSREQVLESVHKHVTLARGYIDDIEFSAEDATRTEEDFLAE
VTRVAIAAGATTINLPDTVGFTTPEEIRGMFSRLIASVEGAEKVIFSTHCHNDLGLAAANSLAAIEGGARQVECTINGIG
ERAGNCALEEITMALKVRGAFYNLDTAINTPRIVSTSQLLQRLVGMPVQRNKAVVGGNAFAHESGIHQHGMLRHRGTYEI
MRPEDVGWESSQMVLGRHSGRAAVEQRLRALGYLLEEDEAKLVFEQFKALCEKQRVVTDADLQALMQDATVQEGYRLASM
TISDVGSRANALVELSDPDGNRVAETAQGNGPVDALFGALASATGVKLELDSYQVHSVGIGADARGEASLSVRHDGVEYE
GTGTSKDIIEASALAWLDVANRLLRQRERGVVAGKTAAVA

Sequences:

>Translated_520_residues
MNTTVSNQTPHIRIFDTTLRDGEQSPGCSMTPQQKLVMARALDALGVDIIETGFPASSYSDREAVAMMGRELRRPTLAVL
SRCLQADIEISARALEAAANPRLHVFLSTSPLHREHKLRMSREQVLESVHKHVTLARGYIDDIEFSAEDATRTEEDFLAE
VTRVAIAAGATTINLPDTVGFTTPEEIRGMFSRLIASVEGAEKVIFSTHCHNDLGLAAANSLAAIEGGARQVECTINGIG
ERAGNCALEEITMALKVRGAFYNLDTAINTPRIVSTSQLLQRLVGMPVQRNKAVVGGNAFAHESGIHQHGMLRHRGTYEI
MRPEDVGWESSQMVLGRHSGRAAVEQRLRALGYLLEEDEAKLVFEQFKALCEKQRVVTDADLQALMQDATVQEGYRLASM
TISDVGSRANALVELSDPDGNRVAETAQGNGPVDALFGALASATGVKLELDSYQVHSVGIGADARGEASLSVRHDGVEYE
GTGTSKDIIEASALAWLDVANRLLRQRERGVVAGKTAAVA
>Mature_520_residues
MNTTVSNQTPHIRIFDTTLRDGEQSPGCSMTPQQKLVMARALDALGVDIIETGFPASSYSDREAVAMMGRELRRPTLAVL
SRCLQADIEISARALEAAANPRLHVFLSTSPLHREHKLRMSREQVLESVHKHVTLARGYIDDIEFSAEDATRTEEDFLAE
VTRVAIAAGATTINLPDTVGFTTPEEIRGMFSRLIASVEGAEKVIFSTHCHNDLGLAAANSLAAIEGGARQVECTINGIG
ERAGNCALEEITMALKVRGAFYNLDTAINTPRIVSTSQLLQRLVGMPVQRNKAVVGGNAFAHESGIHQHGMLRHRGTYEI
MRPEDVGWESSQMVLGRHSGRAAVEQRLRALGYLLEEDEAKLVFEQFKALCEKQRVVTDADLQALMQDATVQEGYRLASM
TISDVGSRANALVELSDPDGNRVAETAQGNGPVDALFGALASATGVKLELDSYQVHSVGIGADARGEASLSVRHDGVEYE
GTGTSKDIIEASALAWLDVANRLLRQRERGVVAGKTAAVA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=504, Percent_Identity=44.8412698412698, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6320071, Length=381, Percent_Identity=28.0839895013123, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6320019, Length=381, Percent_Identity=27.8215223097113, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324682, Length=547, Percent_Identity=25.9597806215722, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=580, Percent_Identity=24.8275862068966, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24582381, Length=297, Percent_Identity=23.5690235690236, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56222; Mature: 56222

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTVSNQTPHIRIFDTTLRDGEQSPGCSMTPQQKLVMARALDALGVDIIETGFPASSYS
CCCCCCCCCCEEEEEECHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
DREAVAMMGRELRRPTLAVLSRCLQADIEISARALEAAANPRLHVFLSTSPLHREHKLRM
CHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
SREQVLESVHKHVTLARGYIDDIEFSAEDATRTEEDFLAEVTRVAIAAGATTINLPDTVG
HHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCC
FTTPEEIRGMFSRLIASVEGAEKVIFSTHCHNDLGLAAANSLAAIEGGARQVECTINGIG
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHCCHHEECCCCEEEEEEEECCC
ERAGNCALEEITMALKVRGAFYNLDTAINTPRIVSTSQLLQRLVGMPVQRNKAVVGGNAF
CCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCEECHHHHHHHHHCCCCCCCCEEECCCCH
AHESGIHQHGMLRHRGTYEIMRPEDVGWESSQMVLGRHSGRAAVEQRLRALGYLLEEDEA
HHCCCCCCCCCHHCCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCHH
KLVFEQFKALCEKQRVVTDADLQALMQDATVQEGYRLASMTISDVGSRANALVELSDPDG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCEEEEECCCCC
NRVAETAQGNGPVDALFGALASATGVKLELDSYQVHSVGIGADARGEASLSVRHDGVEYE
CCCHHCCCCCCCHHHHHHHHHHCCCCEEEECCEEEEEEECCCCCCCCCEEEEEECCEEEC
GTGTSKDIIEASALAWLDVANRLLRQRERGVVAGKTAAVA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHCCC
>Mature Secondary Structure
MNTTVSNQTPHIRIFDTTLRDGEQSPGCSMTPQQKLVMARALDALGVDIIETGFPASSYS
CCCCCCCCCCEEEEEECHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
DREAVAMMGRELRRPTLAVLSRCLQADIEISARALEAAANPRLHVFLSTSPLHREHKLRM
CHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
SREQVLESVHKHVTLARGYIDDIEFSAEDATRTEEDFLAEVTRVAIAAGATTINLPDTVG
HHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCC
FTTPEEIRGMFSRLIASVEGAEKVIFSTHCHNDLGLAAANSLAAIEGGARQVECTINGIG
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHCCHHEECCCCEEEEEEEECCC
ERAGNCALEEITMALKVRGAFYNLDTAINTPRIVSTSQLLQRLVGMPVQRNKAVVGGNAF
CCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCEECHHHHHHHHHCCCCCCCCEEECCCCH
AHESGIHQHGMLRHRGTYEIMRPEDVGWESSQMVLGRHSGRAAVEQRLRALGYLLEEDEA
HHCCCCCCCCCHHCCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCHH
KLVFEQFKALCEKQRVVTDADLQALMQDATVQEGYRLASMTISDVGSRANALVELSDPDG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCEEEEECCCCC
NRVAETAQGNGPVDALFGALASATGVKLELDSYQVHSVGIGADARGEASLSVRHDGVEYE
CCCHHCCCCCCCHHHHHHHHHHCCCCEEEECCEEEEEEECCCCCCCCCEEEEEECCEEEC
GTGTSKDIIEASALAWLDVANRLLRQRERGVVAGKTAAVA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]