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The map label for this gene is aspA [H]

Identifier: 66048275

GI number: 66048275

Start: 5992080

End: 5993504

Strand: Direct

Name: aspA [H]

Synonym: Psyr_5051

Alternate gene names: 66048275

Gene position: 5992080-5993504 (Clockwise)

Preceding gene: 66048273

Following gene: 66048276

Centisome position: 98.33

GC content: 61.89

Gene sequence:

>1425_bases
ATGTCCTCGCCTGCATCATCGCGCATCGAAAAAGACCTGCTTGGTGTTCTCGAAGTACCTGCCAACGCTTATTACGGCAT
CCAGACCCTGCGAGCGGTGAACAACTTTCACCTCTCCGGCGTGCCACTTTCGCACTACCCGAAACTGGTAGTGGCGCTGG
CCATGGTCAAGCAGGCGGCGGCGGATGCAAACCATCAGCTTGGACACCTCAATGCTGCCAAGCATGCGGCCATCAGCGAG
GCCTGCGCGCGCCTGATCCGCGGCGACTTCCATGATCAGTTCGTGGTCGACATGATCCAGGGCGGCGCTGGCACGTCCAC
CAACATGAATGCCAACGAAGTCATCGCCAACATCGCTCTGGAAACCATGGGTTTCGAGAAAGGCGACTACAAACACCTGC
ACCCCAACAACGATGTCAACATGGCGCAGTCGACCAACGACGCCTACCCCACTGCGATCCGTCTGGGTCTGCTGCTGGGT
CACGACGCCCTGCTCGCCAGCCTGTCCAGCCTGATTCAGGCGTTCGCCGCCAAGGGCGAAGAATTCAACCACGTGCTGAA
GATGGGCCGCACCCAGTTGCAGGACGCCGTTCCGATGACTCTGGGTCAGGAATTCCGCGCCTTCGCCACCACCCTGACCG
AAGACCTCAACCGCCTGCGCAGCCTGGCCCCCGAGCTGTTGACCGAAGTGAACCTGGGCGGAACCGCCATCGGTACCGGC
ATCAACGCCGACCCCGGCTATCAGAAGCTGGCGGTCGATCGTCTGGCGCTCATCAGCGGCCAGCCTCTGGTGCCTGCCGC
CGACCTGATCGAAGCGACCTCCGACATGGGCGCCTTCGTGCTGTTCTCGGGCATGCTCAAGCGTACTGCGGTCAAGCTGT
CGAAAATCTGCAACGACCTGCGTCTGCTGTCCAGCGGACCGCGCACCGGCATCAACGAAATCAACCTGCCGGCACGCCAG
CCAGGCAGCTCGATCATGCCCGGCAAGGTCAACCCGGTGATCCCGGAAGCGGTCAATCAGGTGGCTTTCGAAATCATCGG
CAACGACCTGTCGCTGACCATGGCTGCCGAAGGCGGGCAATTGCAGCTCAACGTGATGGAGCCGCTGATCGCCTACAAGA
TCTTCGACTCGATCCGCCTGCTGCAACGTGCCATGGACATGCTGCGCGAGCACTGCATCGTCGGCATCACGGCCAACGAA
CAGCGCTGCCGCGAGCTGGTCGAGCATTCGATCGGTCTGGTCACCGCCCTGAACCCTTACATCGGTTACGAGAACTCCAC
CCGTATCGCCCGCATCGCGCTGGAAACCGGCCGCGGCGTGCTGGAACTGGTGCGTGAGGAAGGTCTGCTCGACGACGCCA
TGCTCGACGACATCCTGCGCCCGGAAAACATGATCGCTCCGCGTCTGGCCCCCTTGAAGGCCTGA

Upstream 100 bases:

>100_bases
GCATAGGCCAGCGTTTAACAGCATTGGTCAACCGAGGGCGGCAAGCCCACTATTCGTGGCGTTCCGGCATTGTCCGTGCC
TTTTTGAGACTTTTTTTATC

Downstream 100 bases:

>100_bases
CAGGAATGACCTGCGCAACCTGCTCACCAGATTGAGGGCCTAGACACCCCTCAATTTTTTGAGGGTCTGGGAGTGATTCT
CCAGACCCTTTTTTTTGCCT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSSPASSRIEKDLLGVLEVPANAYYGIQTLRAVNNFHLSGVPLSHYPKLVVALAMVKQAAADANHQLGHLNAAKHAAISE
ACARLIRGDFHDQFVVDMIQGGAGTSTNMNANEVIANIALETMGFEKGDYKHLHPNNDVNMAQSTNDAYPTAIRLGLLLG
HDALLASLSSLIQAFAAKGEEFNHVLKMGRTQLQDAVPMTLGQEFRAFATTLTEDLNRLRSLAPELLTEVNLGGTAIGTG
INADPGYQKLAVDRLALISGQPLVPAADLIEATSDMGAFVLFSGMLKRTAVKLSKICNDLRLLSSGPRTGINEINLPARQ
PGSSIMPGKVNPVIPEAVNQVAFEIIGNDLSLTMAAEGGQLQLNVMEPLIAYKIFDSIRLLQRAMDMLREHCIVGITANE
QRCRELVEHSIGLVTALNPYIGYENSTRIARIALETGRGVLELVREEGLLDDAMLDDILRPENMIAPRLAPLKA

Sequences:

>Translated_474_residues
MSSPASSRIEKDLLGVLEVPANAYYGIQTLRAVNNFHLSGVPLSHYPKLVVALAMVKQAAADANHQLGHLNAAKHAAISE
ACARLIRGDFHDQFVVDMIQGGAGTSTNMNANEVIANIALETMGFEKGDYKHLHPNNDVNMAQSTNDAYPTAIRLGLLLG
HDALLASLSSLIQAFAAKGEEFNHVLKMGRTQLQDAVPMTLGQEFRAFATTLTEDLNRLRSLAPELLTEVNLGGTAIGTG
INADPGYQKLAVDRLALISGQPLVPAADLIEATSDMGAFVLFSGMLKRTAVKLSKICNDLRLLSSGPRTGINEINLPARQ
PGSSIMPGKVNPVIPEAVNQVAFEIIGNDLSLTMAAEGGQLQLNVMEPLIAYKIFDSIRLLQRAMDMLREHCIVGITANE
QRCRELVEHSIGLVTALNPYIGYENSTRIARIALETGRGVLELVREEGLLDDAMLDDILRPENMIAPRLAPLKA
>Mature_473_residues
SSPASSRIEKDLLGVLEVPANAYYGIQTLRAVNNFHLSGVPLSHYPKLVVALAMVKQAAADANHQLGHLNAAKHAAISEA
CARLIRGDFHDQFVVDMIQGGAGTSTNMNANEVIANIALETMGFEKGDYKHLHPNNDVNMAQSTNDAYPTAIRLGLLLGH
DALLASLSSLIQAFAAKGEEFNHVLKMGRTQLQDAVPMTLGQEFRAFATTLTEDLNRLRSLAPELLTEVNLGGTAIGTGI
NADPGYQKLAVDRLALISGQPLVPAADLIEATSDMGAFVLFSGMLKRTAVKLSKICNDLRLLSSGPRTGINEINLPARQP
GSSIMPGKVNPVIPEAVNQVAFEIIGNDLSLTMAAEGGQLQLNVMEPLIAYKIFDSIRLLQRAMDMLREHCIVGITANEQ
RCRELVEHSIGLVTALNPYIGYENSTRIARIALETGRGVLELVREEGLLDDAMLDDILRPENMIAPRLAPLKA

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=469, Percent_Identity=40.724946695096, Blast_Score=358, Evalue=7e-99,
Organism=Escherichia coli, GI87082375, Length=461, Percent_Identity=57.0498915401301, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI1787896, Length=461, Percent_Identity=41.2147505422993, Blast_Score=359, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17553882, Length=468, Percent_Identity=41.025641025641, Blast_Score=356, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI32565146, Length=331, Percent_Identity=40.1812688821752, Blast_Score=260, Evalue=9e-70,
Organism=Saccharomyces cerevisiae, GI6324993, Length=465, Percent_Identity=40.2150537634409, Blast_Score=348, Evalue=8e-97,
Organism=Drosophila melanogaster, GI24640177, Length=464, Percent_Identity=40.5172413793103, Blast_Score=349, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24640179, Length=464, Percent_Identity=40.5172413793103, Blast_Score=349, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=39.5698924731183, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=38.5775862068966, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24583245, Length=467, Percent_Identity=36.1884368308351, Blast_Score=302, Evalue=3e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51118; Mature: 50986

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPASSRIEKDLLGVLEVPANAYYGIQTLRAVNNFHLSGVPLSHYPKLVVALAMVKQAA
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ADANHQLGHLNAAKHAAISEACARLIRGDFHDQFVVDMIQGGAGTSTNMNANEVIANIAL
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ETMGFEKGDYKHLHPNNDVNMAQSTNDAYPTAIRLGLLLGHDALLASLSSLIQAFAAKGE
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EFNHVLKMGRTQLQDAVPMTLGQEFRAFATTLTEDLNRLRSLAPELLTEVNLGGTAIGTG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCC
INADPGYQKLAVDRLALISGQPLVPAADLIEATSDMGAFVLFSGMLKRTAVKLSKICNDL
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
RLLSSGPRTGINEINLPARQPGSSIMPGKVNPVIPEAVNQVAFEIIGNDLSLTMAAEGGQ
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCE
LQLNVMEPLIAYKIFDSIRLLQRAMDMLREHCIVGITANEQRCRELVEHSIGLVTALNPY
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHCCHHEECCCC
IGYENSTRIARIALETGRGVLELVREEGLLDDAMLDDILRPENMIAPRLAPLKA
CCCCCCCEEEEEEEHHCCHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCCCCCCC
>Mature Secondary Structure 
SSPASSRIEKDLLGVLEVPANAYYGIQTLRAVNNFHLSGVPLSHYPKLVVALAMVKQAA
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ADANHQLGHLNAAKHAAISEACARLIRGDFHDQFVVDMIQGGAGTSTNMNANEVIANIAL
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ETMGFEKGDYKHLHPNNDVNMAQSTNDAYPTAIRLGLLLGHDALLASLSSLIQAFAAKGE
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EFNHVLKMGRTQLQDAVPMTLGQEFRAFATTLTEDLNRLRSLAPELLTEVNLGGTAIGTG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCC
INADPGYQKLAVDRLALISGQPLVPAADLIEATSDMGAFVLFSGMLKRTAVKLSKICNDL
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
RLLSSGPRTGINEINLPARQPGSSIMPGKVNPVIPEAVNQVAFEIIGNDLSLTMAAEGGQ
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCE
LQLNVMEPLIAYKIFDSIRLLQRAMDMLREHCIVGITANEQRCRELVEHSIGLVTALNPY
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHCCHHEECCCC
IGYENSTRIARIALETGRGVLELVREEGLLDDAMLDDILRPENMIAPRLAPLKA
CCCCCCCEEEEEEEHHCCHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]