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The map label for this gene is artJ [H]

Identifier: 66048136

GI number: 66048136

Start: 5824402

End: 5825166

Strand: Direct

Name: artJ [H]

Synonym: Psyr_4912

Alternate gene names: 66048136

Gene position: 5824402-5825166 (Clockwise)

Preceding gene: 66048135

Following gene: 66048137

Centisome position: 95.58

GC content: 56.47

Gene sequence:

>765_bases
ATGCAATCGTACAAAAAGATCTTGCTGATCGCAGCGGCCACCCTTGCCTTCAGCGGCAGTGCCTTTGCTGAAACCATGAA
AATGGGCATCGAAGGGGCCTACCCGCCGTTCAACAACAAGGACGCGAGTGGCCAGGTGGTAGGCTTCGACTATGAAATCG
GCCAGGCCCTGTGCGCCAAGATGAAAGTCGAGTGCGAAGCGGTGACGTCAGACTGGGACGGCATCATCCCGGCGTTGATG
GCCAACAAGTTCGACTTCCTGATCTCGTCACTGTCGATCACCGAAGAGCGCAAGCAGGTGGTCGATTTCACCAACCCGTA
TTACTCGAACAAGCTGCAATTCATCGCGCCGAAAACCTCCTCGATCGACGTATCGAGCGTTGAAAAAGCCAAAGAGAGCC
TTGCCGGCAAGTCAGTGGGTGCACAGCGCTCGACGCTGGCCGCCACCTGGCTGGAAGACAAGCTCGGGATCATGGACGCC
AAACTCTATGACACTCAGGAAAACGCCTTCCTCGACCTGTCCTCCGGTCGCGTCGACGCCATGCTGGCCGACAAATACGT
CAGCTTCGAATGGCTGAAAAGCGAAGCCGGTCAGAACTTCGAGTTCAAGGGCGAGCCTGTCGTGGAAGACGACAAGATCG
GCATCGCCGTGCGCAAGGGTGACGACGCCCTGCGCGAGCGCTTGAACGCGGCCCTGAAAGAAATCATCGCTGACGGTACC
TACAAGAAGATCAACGACAAGTATTTCCCTTTCAGCATCCTCTGA

Upstream 100 bases:

>100_bases
ATGGTCGAAGAACAGGGCACACCGCAGCAAGTATTCGAAAACCCGCTGTCGGCAAGGTGCAAACAATTCATGTCGAGTCA
CCGCTAACGGAGCAACACCC

Downstream 100 bases:

>100_bases
TTCACCTGACCGCGTCGCCGCCGCTTGCTGTCGAAGCGCGGCGACCGGCCCTGACAAAGCCTGCCCATGAACATTGATCT
CCACGGATTCGGCCCGGCCC

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MQSYKKILLIAAATLAFSGSAFAETMKMGIEGAYPPFNNKDASGQVVGFDYEIGQALCAKMKVECEAVTSDWDGIIPALM
ANKFDFLISSLSITEERKQVVDFTNPYYSNKLQFIAPKTSSIDVSSVEKAKESLAGKSVGAQRSTLAATWLEDKLGIMDA
KLYDTQENAFLDLSSGRVDAMLADKYVSFEWLKSEAGQNFEFKGEPVVEDDKIGIAVRKGDDALRERLNAALKEIIADGT
YKKINDKYFPFSIL

Sequences:

>Translated_254_residues
MQSYKKILLIAAATLAFSGSAFAETMKMGIEGAYPPFNNKDASGQVVGFDYEIGQALCAKMKVECEAVTSDWDGIIPALM
ANKFDFLISSLSITEERKQVVDFTNPYYSNKLQFIAPKTSSIDVSSVEKAKESLAGKSVGAQRSTLAATWLEDKLGIMDA
KLYDTQENAFLDLSSGRVDAMLADKYVSFEWLKSEAGQNFEFKGEPVVEDDKIGIAVRKGDDALRERLNAALKEIIADGT
YKKINDKYFPFSIL
>Mature_254_residues
MQSYKKILLIAAATLAFSGSAFAETMKMGIEGAYPPFNNKDASGQVVGFDYEIGQALCAKMKVECEAVTSDWDGIIPALM
ANKFDFLISSLSITEERKQVVDFTNPYYSNKLQFIAPKTSSIDVSSVEKAKESLAGKSVGAQRSTLAATWLEDKLGIMDA
KLYDTQENAFLDLSSGRVDAMLADKYVSFEWLKSEAGQNFEFKGEPVVEDDKIGIAVRKGDDALRERLNAALKEIIADGT
YKKINDKYFPFSIL

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=248, Percent_Identity=38.3064516129032, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1788649, Length=256, Percent_Identity=39.453125, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1787088, Length=251, Percent_Identity=37.4501992031873, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1788648, Length=241, Percent_Identity=36.5145228215768, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1788228, Length=225, Percent_Identity=30.6666666666667, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787031, Length=251, Percent_Identity=33.0677290836653, Blast_Score=124, Evalue=7e-30,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27988; Mature: 27988

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSYKKILLIAAATLAFSGSAFAETMKMGIEGAYPPFNNKDASGQVVGFDYEIGQALCAK
CCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHH
MKVECEAVTSDWDGIIPALMANKFDFLISSLSITEERKQVVDFTNPYYSNKLQFIAPKTS
HHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
SIDVSSVEKAKESLAGKSVGAQRSTLAATWLEDKLGIMDAKLYDTQENAFLDLSSGRVDA
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCEEE
MLADKYVSFEWLKSEAGQNFEFKGEPVVEDDKIGIAVRKGDDALRERLNAALKEIIADGT
EHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCC
YKKINDKYFPFSIL
CHHCCCCCCCEEEC
>Mature Secondary Structure
MQSYKKILLIAAATLAFSGSAFAETMKMGIEGAYPPFNNKDASGQVVGFDYEIGQALCAK
CCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHH
MKVECEAVTSDWDGIIPALMANKFDFLISSLSITEERKQVVDFTNPYYSNKLQFIAPKTS
HHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
SIDVSSVEKAKESLAGKSVGAQRSTLAATWLEDKLGIMDAKLYDTQENAFLDLSSGRVDA
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCEEE
MLADKYVSFEWLKSEAGQNFEFKGEPVVEDDKIGIAVRKGDDALRERLNAALKEIIADGT
EHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCC
YKKINDKYFPFSIL
CHHCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]