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The map label for this gene is lptD [H]

Identifier: 66047851

GI number: 66047851

Start: 5482570

End: 5485350

Strand: Direct

Name: lptD [H]

Synonym: Psyr_4624

Alternate gene names: 66047851

Gene position: 5482570-5485350 (Clockwise)

Preceding gene: 66047847

Following gene: 66047852

Centisome position: 89.97

GC content: 58.58

Gene sequence:

>2781_bases
ATGGCATTGAAATCCCCCGCGTTTCGTAAAAAATTTCCGTTGCTCGTAACCGGCAGTCTGCTGGCGATGCAACCTCTGGC
CACTCAGTTCGTGGTCGCCGCGGAACAGTATGACTGCTCAGTCTCTGCTTCGGGTGCCTGGAACTGTGCGCCGAAATCCA
ATGCTGCTGCAGTGGATCTGCCTCCGCGCCCCGTGCATGACACGACGTCGGTCAGCTCCAACGGCACGGTCACCTCACAA
GGCACCTCTTCGGGTGAACAGTCTGCGGGTACGCAACTGGTCACTGAAGCCAAGGGCAAAGGTCTGAAGTCGCGTAGTGC
TGACTACAGCCACCTCGATTGGGTTCCTCGCGAAAAGCTTACCGCAGCGCAACTGGCCGAGACCGGCCCGTATTGCTCGG
GTGCCTACGTCGAGCCGATCCGTCCTGGCATGGACGACAAGACGCCGATGAAAGACGCGCCGATGTTCGTGGGAGCCAAG
GCTTCGCGTTACGAACAGGACGCGCAGGTCGCGACGCTGGCCGGTGATGTGGTGATGCGCCAGGGCAGCATGCAGGTGCA
GGCCCAGGAGGCTGCACTGCATCAGGCCGAGAACCGTGGCGAGCTGAACGGCGATGTCCGTCTGCGCGACAACGGTGCGC
TGATCGTCGGTGACAAGGCCGAACTGCAACTCGACACCGGCGAAGCCCGTGTCGACAACGCCGAGTACGTCCTGCACAAG
TCGAACATTCGCGGTAACGCCCTGTACGCCAAGCGTGCAGAGAACGCGATCATCCGGCTCAAGGACGGTACCTACACGAC
GTGCGAGCCGAACAGCAACGCCTGGACGCTCAAGGGCAACAACATCACGCTGAACCCGGCCACCGGCTTCGGTACAGCGA
CCAACGTTACCCTGCGGGTCAAGGACATACCGGTTCTGTACACGCCGTATATCTACTTCCCGATCGACGACCGTCGTCAG
TCCGGTTTCCTGCCGCCGACCATTGCAGCAGGTGGCGACAACGGCTTCACGCTGGTCACCCCGTACTACTTCAACCTGGC
ACCGAACTACGACGCCACGTTGTACCCGCGTTACATGGCTGATCGTGGCTTGCTGATGGAAGGCGAGTTCCGCTACCTGA
CCAAGGGCAGCGAAGGTCAGTTTGGCGGCGCGTACCTGAACGACGAGAATGACGACCGCAAACAGCAGTCCGATTACGAC
AAGACCCGCTGGATGATCAACTGGCAGCACAAGGGCGGGCTTGATACGCGCTGGCTGACCCAGGTCGATTACACCGATAT
CAGTGATCCGTATTACTTCCAGGATCTGGAAACCGATCAGATCGGCGTAAAAAGAACAGACTTCCTCAACCAGCAGGGCT
CCCTGACCTACCGTGGCGACAGCTTCTCGGCAGTGCTCAATGCTCAGGCCTACAAGCTGGCGACGGTTGCCAACGTTACG
CCGTACAACCGCCTGCCTCAGCTGACGCTCAACGGTACGTTGCCCTACAACCCCGGCGGCCTGAAGTTCGATTATCAGAC
CGAAGCCGTCCGCTTCGAGCGTGATCTGCGCAGCGGCGCGTTCATCGATGAAGATGGCAACTCCGAAACACGTCTGGACA
ACAACATCTCCGGCCTGGCTCGCGCCAACGGTGATCGCCTGAACCTGGCACCGTCGGTCAGCTTGCCGATGAACTGGACC
TATGGCTTCCTGACGCCGAAGCTGAAGTATGTCTACACGCAATACGATCTGGATCTGGACAGCCAGGGCAAAAACACCCT
GCTGGCCGGCGAAGAGTACAGCAGCAGCCAAAGCCGCAGCGTACCGATCTTCAGCGTCGACAGCGGTCTGTACTTCGACC
GCAATACCAACTGGTTCGGCAAGGATTACCGCCAGACCCTGGAGCCGCGCCTGTTCTATCTCTATGTTCCCGAGAAAGAC
CAGACCGATATCCCGGTATTCGACACCAGCGAAAGCACGTTCAACTATGCCTCGCTGTTCCGTGACAACCGTTTCACCGG
CTCCGACCGCATCGGCGACGAGAACAAACTGTCGCTGGGCGTCACCAACCGCTGGATCGAGGACAACGGCTTCGAACGTC
AACGCTTCAGCATCGGTCAGGCGCTGTATTTCGAAGACCGCAAGGTGCAGTTGCCAGGCGTGGTGTTTGCTGATCGTGAT
GATGCGCGTTCCAACGTTTCGCCTTACGCACTGGAATACGAATACCGCTTCAACCGCGACTGGCGCTTCAACTCCGATTT
CAACTGGGATCCGGACAGCCGAAGCACCCGTTCGGGCAGCGCAATGTTCCACTACCAGCCTGAAGACAACCCGAACAAGG
TCGTCAACCTCGGCTATCGCTATCGTAACGACCAGATTCGTTACGACGAAAGCACCGGCCGTTGGGTCGTGGGCGGTGGC
GACTACGGCACTCCGGGTTCGCCAAACTACGTGAAGGACTACTACAAGATTCAGCAGCACGATTTCTCGGTCATCTGGCC
GATCGTGCCGCAGTGGAGCCTGATCAGCCGTTGGCAGTATGACTACAACCGTGAACGCACCATCGAAGCTTTCGGCGGGT
TCGAGTACGACAACTGCTGCTGGAAAATGCGCCTGGTCAATCGTTACTGGATCGACTACGACGAATTCAGCCAGGCAGCA
CCTCAGAACGAGAAAGGCGACCGCGGCATATTCCTACAAATCGTGTTGAAGGGGCTCGGCGGTGTAACTGGCGCCAAGGT
AGACAGCTTCCTCGACAAAGGCATCCAAGGTTATCGTGAACGTGAAGACCAAGCTTTCTGA

Upstream 100 bases:

>100_bases
AAGCGGGTCATGCTTTATTATCCGGAATCTTTTTCAGCCCATCGAGAGGCGTGCGGCCCCCCCGCGGGCAGATGGCGCGC
AGGAAGCCCGGACTAATAAG

Downstream 100 bases:

>100_bases
TTGTCTGCGCCCGCTGATGCTGGGCGCGTTACTCCTGAGCGGTGCAGTGCATGCTGCGGTTCAACCTCTGGACAGTGTCG
TGGCCATCGTCGACAACGAC

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 926; Mature: 925

Protein sequence:

>926_residues
MALKSPAFRKKFPLLVTGSLLAMQPLATQFVVAAEQYDCSVSASGAWNCAPKSNAAAVDLPPRPVHDTTSVSSNGTVTSQ
GTSSGEQSAGTQLVTEAKGKGLKSRSADYSHLDWVPREKLTAAQLAETGPYCSGAYVEPIRPGMDDKTPMKDAPMFVGAK
ASRYEQDAQVATLAGDVVMRQGSMQVQAQEAALHQAENRGELNGDVRLRDNGALIVGDKAELQLDTGEARVDNAEYVLHK
SNIRGNALYAKRAENAIIRLKDGTYTTCEPNSNAWTLKGNNITLNPATGFGTATNVTLRVKDIPVLYTPYIYFPIDDRRQ
SGFLPPTIAAGGDNGFTLVTPYYFNLAPNYDATLYPRYMADRGLLMEGEFRYLTKGSEGQFGGAYLNDENDDRKQQSDYD
KTRWMINWQHKGGLDTRWLTQVDYTDISDPYYFQDLETDQIGVKRTDFLNQQGSLTYRGDSFSAVLNAQAYKLATVANVT
PYNRLPQLTLNGTLPYNPGGLKFDYQTEAVRFERDLRSGAFIDEDGNSETRLDNNISGLARANGDRLNLAPSVSLPMNWT
YGFLTPKLKYVYTQYDLDLDSQGKNTLLAGEEYSSSQSRSVPIFSVDSGLYFDRNTNWFGKDYRQTLEPRLFYLYVPEKD
QTDIPVFDTSESTFNYASLFRDNRFTGSDRIGDENKLSLGVTNRWIEDNGFERQRFSIGQALYFEDRKVQLPGVVFADRD
DARSNVSPYALEYEYRFNRDWRFNSDFNWDPDSRSTRSGSAMFHYQPEDNPNKVVNLGYRYRNDQIRYDESTGRWVVGGG
DYGTPGSPNYVKDYYKIQQHDFSVIWPIVPQWSLISRWQYDYNRERTIEAFGGFEYDNCCWKMRLVNRYWIDYDEFSQAA
PQNEKGDRGIFLQIVLKGLGGVTGAKVDSFLDKGIQGYREREDQAF

Sequences:

>Translated_926_residues
MALKSPAFRKKFPLLVTGSLLAMQPLATQFVVAAEQYDCSVSASGAWNCAPKSNAAAVDLPPRPVHDTTSVSSNGTVTSQ
GTSSGEQSAGTQLVTEAKGKGLKSRSADYSHLDWVPREKLTAAQLAETGPYCSGAYVEPIRPGMDDKTPMKDAPMFVGAK
ASRYEQDAQVATLAGDVVMRQGSMQVQAQEAALHQAENRGELNGDVRLRDNGALIVGDKAELQLDTGEARVDNAEYVLHK
SNIRGNALYAKRAENAIIRLKDGTYTTCEPNSNAWTLKGNNITLNPATGFGTATNVTLRVKDIPVLYTPYIYFPIDDRRQ
SGFLPPTIAAGGDNGFTLVTPYYFNLAPNYDATLYPRYMADRGLLMEGEFRYLTKGSEGQFGGAYLNDENDDRKQQSDYD
KTRWMINWQHKGGLDTRWLTQVDYTDISDPYYFQDLETDQIGVKRTDFLNQQGSLTYRGDSFSAVLNAQAYKLATVANVT
PYNRLPQLTLNGTLPYNPGGLKFDYQTEAVRFERDLRSGAFIDEDGNSETRLDNNISGLARANGDRLNLAPSVSLPMNWT
YGFLTPKLKYVYTQYDLDLDSQGKNTLLAGEEYSSSQSRSVPIFSVDSGLYFDRNTNWFGKDYRQTLEPRLFYLYVPEKD
QTDIPVFDTSESTFNYASLFRDNRFTGSDRIGDENKLSLGVTNRWIEDNGFERQRFSIGQALYFEDRKVQLPGVVFADRD
DARSNVSPYALEYEYRFNRDWRFNSDFNWDPDSRSTRSGSAMFHYQPEDNPNKVVNLGYRYRNDQIRYDESTGRWVVGGG
DYGTPGSPNYVKDYYKIQQHDFSVIWPIVPQWSLISRWQYDYNRERTIEAFGGFEYDNCCWKMRLVNRYWIDYDEFSQAA
PQNEKGDRGIFLQIVLKGLGGVTGAKVDSFLDKGIQGYREREDQAF
>Mature_925_residues
ALKSPAFRKKFPLLVTGSLLAMQPLATQFVVAAEQYDCSVSASGAWNCAPKSNAAAVDLPPRPVHDTTSVSSNGTVTSQG
TSSGEQSAGTQLVTEAKGKGLKSRSADYSHLDWVPREKLTAAQLAETGPYCSGAYVEPIRPGMDDKTPMKDAPMFVGAKA
SRYEQDAQVATLAGDVVMRQGSMQVQAQEAALHQAENRGELNGDVRLRDNGALIVGDKAELQLDTGEARVDNAEYVLHKS
NIRGNALYAKRAENAIIRLKDGTYTTCEPNSNAWTLKGNNITLNPATGFGTATNVTLRVKDIPVLYTPYIYFPIDDRRQS
GFLPPTIAAGGDNGFTLVTPYYFNLAPNYDATLYPRYMADRGLLMEGEFRYLTKGSEGQFGGAYLNDENDDRKQQSDYDK
TRWMINWQHKGGLDTRWLTQVDYTDISDPYYFQDLETDQIGVKRTDFLNQQGSLTYRGDSFSAVLNAQAYKLATVANVTP
YNRLPQLTLNGTLPYNPGGLKFDYQTEAVRFERDLRSGAFIDEDGNSETRLDNNISGLARANGDRLNLAPSVSLPMNWTY
GFLTPKLKYVYTQYDLDLDSQGKNTLLAGEEYSSSQSRSVPIFSVDSGLYFDRNTNWFGKDYRQTLEPRLFYLYVPEKDQ
TDIPVFDTSESTFNYASLFRDNRFTGSDRIGDENKLSLGVTNRWIEDNGFERQRFSIGQALYFEDRKVQLPGVVFADRDD
ARSNVSPYALEYEYRFNRDWRFNSDFNWDPDSRSTRSGSAMFHYQPEDNPNKVVNLGYRYRNDQIRYDESTGRWVVGGGD
YGTPGSPNYVKDYYKIQQHDFSVIWPIVPQWSLISRWQYDYNRERTIEAFGGFEYDNCCWKMRLVNRYWIDYDEFSQAAP
QNEKGDRGIFLQIVLKGLGGVTGAKVDSFLDKGIQGYREREDQAF

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=820, Percent_Identity=29.2682926829268, Blast_Score=314, Evalue=1e-86,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 104581; Mature: 104450

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKSPAFRKKFPLLVTGSLLAMQPLATQFVVAAEQYDCSVSASGAWNCAPKSNAAAVDL
CCCCCCCHHHCCCEEEECCHHHHHHHHHEEEEEEECCCCEEECCCCEECCCCCCCEEEEC
PPRPVHDTTSVSSNGTVTSQGTSSGEQSAGTQLVTEAKGKGLKSRSADYSHLDWVPREKL
CCCCCCCCCCCCCCCEEEECCCCCCCHHCCCEEEEHHCCCCCCCCCCCCCCCCCCCHHHH
TAAQLAETGPYCSGAYVEPIRPGMDDKTPMKDAPMFVGAKASRYEQDAQVATLAGDVVMR
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHCCCCCCEEEEEECHHEEE
QGSMQVQAQEAALHQAENRGELNGDVRLRDNGALIVGDKAELQLDTGEARVDNAEYVLHK
CCCCEEEHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCEEEEECCCCCCCCCEEEEEE
SNIRGNALYAKRAENAIIRLKDGTYTTCEPNSNAWTLKGNNITLNPATGFGTATNVTLRV
CCCCCCEEEEECCCCEEEEECCCCEEEECCCCCEEEEECCEEEECCCCCCCCEEEEEEEE
KDIPVLYTPYIYFPIDDRRQSGFLPPTIAAGGDNGFTLVTPYYFNLAPNYDATLYPRYMA
ECCCEEECCEEEEECCCCCCCCCCCCEEECCCCCCEEEECEEEEEECCCCCCEECCHHHC
DRGLLMEGEFRYLTKGSEGQFGGAYLNDENDDRKQQSDYDKTRWMINWQHKGGLDTRWLT
CCCEEEECCEEEEECCCCCCCCCEEECCCCCCHHHHCCCCCEEEEEEEECCCCCCCEEEE
QVDYTDISDPYYFQDLETDQIGVKRTDFLNQQGSLTYRGDSFSAVLNAQAYKLATVANVT
EECCCCCCCCEEEECCCCCCCCCCHHHHCCCCCCEEECCCCEEEEECCCEEEEEEEECCC
PYNRLPQLTLNGTLPYNPGGLKFDYQTEAVRFERDLRSGAFIDEDGNSETRLDNNISGLA
CCCCCCEEEECCCCCCCCCCEEEECCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCHH
RANGDRLNLAPSVSLPMNWTYGFLTPKLKYVYTQYDLDLDSQGKNTLLAGEEYSSSQSRS
HCCCCEEEECCCCCCCCCCEEEECCCCEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCC
VPIFSVDSGLYFDRNTNWFGKDYRQTLEPRLFYLYVPEKDQTDIPVFDTSESTFNYASLF
CEEEEECCCEEEECCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCHHHHHH
RDNRFTGSDRIGDENKLSLGVTNRWIEDNGFERQRFSIGQALYFEDRKVQLPGVVFADRD
CCCCCCCCCCCCCCCEEEEEEEECEECCCCCCHHHHHCCEEEEEECCEEECCEEEEECCC
DARSNVSPYALEYEYRFNRDWRFNSDFNWDPDSRSTRSGSAMFHYQPEDNPNKVVNLGYR
CCCCCCCCEEEEEEEEECCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEE
YRNDQIRYDESTGRWVVGGGDYGTPGSPNYVKDYYKIQQHDFSVIWPIVPQWSLISRWQY
ECCCEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCC
DYNRERTIEAFGGFEYDNCCWKMRLVNRYWIDYDEFSQAAPQNEKGDRGIFLQIVLKGLG
CCCCCEEEHHHCCCCCCCCEEEEEEHHHEECCHHHHHHCCCCCCCCCCCEEHEEHHHHHC
GVTGAKVDSFLDKGIQGYREREDQAF
CCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALKSPAFRKKFPLLVTGSLLAMQPLATQFVVAAEQYDCSVSASGAWNCAPKSNAAAVDL
CCCCCCHHHCCCEEEECCHHHHHHHHHEEEEEEECCCCEEECCCCEECCCCCCCEEEEC
PPRPVHDTTSVSSNGTVTSQGTSSGEQSAGTQLVTEAKGKGLKSRSADYSHLDWVPREKL
CCCCCCCCCCCCCCCEEEECCCCCCCHHCCCEEEEHHCCCCCCCCCCCCCCCCCCCHHHH
TAAQLAETGPYCSGAYVEPIRPGMDDKTPMKDAPMFVGAKASRYEQDAQVATLAGDVVMR
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHCCCCCCEEEEEECHHEEE
QGSMQVQAQEAALHQAENRGELNGDVRLRDNGALIVGDKAELQLDTGEARVDNAEYVLHK
CCCCEEEHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCEEEEECCCCCCCCCEEEEEE
SNIRGNALYAKRAENAIIRLKDGTYTTCEPNSNAWTLKGNNITLNPATGFGTATNVTLRV
CCCCCCEEEEECCCCEEEEECCCCEEEECCCCCEEEEECCEEEECCCCCCCCEEEEEEEE
KDIPVLYTPYIYFPIDDRRQSGFLPPTIAAGGDNGFTLVTPYYFNLAPNYDATLYPRYMA
ECCCEEECCEEEEECCCCCCCCCCCCEEECCCCCCEEEECEEEEEECCCCCCEECCHHHC
DRGLLMEGEFRYLTKGSEGQFGGAYLNDENDDRKQQSDYDKTRWMINWQHKGGLDTRWLT
CCCEEEECCEEEEECCCCCCCCCEEECCCCCCHHHHCCCCCEEEEEEEECCCCCCCEEEE
QVDYTDISDPYYFQDLETDQIGVKRTDFLNQQGSLTYRGDSFSAVLNAQAYKLATVANVT
EECCCCCCCCEEEECCCCCCCCCCHHHHCCCCCCEEECCCCEEEEECCCEEEEEEEECCC
PYNRLPQLTLNGTLPYNPGGLKFDYQTEAVRFERDLRSGAFIDEDGNSETRLDNNISGLA
CCCCCCEEEECCCCCCCCCCEEEECCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCHH
RANGDRLNLAPSVSLPMNWTYGFLTPKLKYVYTQYDLDLDSQGKNTLLAGEEYSSSQSRS
HCCCCEEEECCCCCCCCCCEEEECCCCEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCC
VPIFSVDSGLYFDRNTNWFGKDYRQTLEPRLFYLYVPEKDQTDIPVFDTSESTFNYASLF
CEEEEECCCEEEECCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCHHHHHH
RDNRFTGSDRIGDENKLSLGVTNRWIEDNGFERQRFSIGQALYFEDRKVQLPGVVFADRD
CCCCCCCCCCCCCCCEEEEEEEECEECCCCCCHHHHHCCEEEEEECCEEECCEEEEECCC
DARSNVSPYALEYEYRFNRDWRFNSDFNWDPDSRSTRSGSAMFHYQPEDNPNKVVNLGYR
CCCCCCCCEEEEEEEEECCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEE
YRNDQIRYDESTGRWVVGGGDYGTPGSPNYVKDYYKIQQHDFSVIWPIVPQWSLISRWQY
ECCCEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCC
DYNRERTIEAFGGFEYDNCCWKMRLVNRYWIDYDEFSQAAPQNEKGDRGIFLQIVLKGLG
CCCCCEEEHHHCCCCCCCCEEEEEEHHHEECCHHHHHHCCCCCCCCCCCEEHEEHHHHHC
GVTGAKVDSFLDKGIQGYREREDQAF
CCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]