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The map label for this gene is mltB [H]

Identifier: 66047590

GI number: 66047590

Start: 5216395

End: 5217402

Strand: Reverse

Name: mltB [H]

Synonym: Psyr_4363

Alternate gene names: 66047590

Gene position: 5217402-5216395 (Counterclockwise)

Preceding gene: 66047591

Following gene: 66047589

Centisome position: 85.62

GC content: 60.71

Gene sequence:

>1008_bases
ATGCAAGCAATGCGTAACTGGGCTCGATATGCTCCGGTGGTCGGTCTGGTGGGCATCCTTGGCTCGGTGCAAGAGGCGCT
GGCGGGCGACTATGATGGGTCGCCCCAGGTGGCGGAATTCGTCGGTGAGCTGACCCGCGATCATGGCTTTGCCGGTGAGC
AGTTGATGAGCCTGTTCCGCGAGGTCGAGCGCAAGCAGGCCATTCTCGATGCCATTTCCCGCCCGGCCGAACGGGTCAAG
CAGTGGAAAGAGTATCGGCCGATGTTCATCACCGATACGCGCATCGCTCGCGGTGTCGACTTCTGGCGTCAGCATGAGGC
CGCCTTGGCGCGTGCCGAGCAGGAATATGGCGTGCCGGCGCAGATCATCGTGTCGATCATTGGTGTAGAAACCTTCTTCG
GCCGCAATACCGGTAATTACCGGGTGATCGATGCCCTGTCGACGCTTGGCTTCGATTACCCGCCCCGCGCCCAGTTCTTC
CGCAAGGAACTGCGCGAGTTTCTGCTGCTGGCGCGCGAGCAACAGGTCGATCCCCTGACCCTCAAGGGGTCGTACGCCGG
GGCGATGGGGCTGCCGCAGTTCATGCCGAGCAGTTTCCGCGCCTACGCGGTGGATTTCGACGGTGACGGTCACATCAATA
TCTGGACCGACCCGGACGATGCCATCGGCAGTGTCGCCAGCTATTTCAAACGCCATGGCTGGGTGGCCGGTCAGCCGGTG
GTCAGTCGCGCCACGGCCCGCGGCGACCGGGTTGACGAGGGCTTGAGTCCTGCGCTTGATCCGGTAATGTCGGTCGGGGA
GTTGCGACAGCTGGGCTGGGCGAGTCATGATGCGCTGCGCGACGATATGCCGGTGACGGCATTTCGTCTGGAAGGCGACA
ATGGTCCTGAATACTGGCTGGGTTTGAACAATTTTTATGCAATCACCCGTTATAACCGCAGTGTGATGTACGCCATGGCC
GTATATCAGCTGTCTGAATCGTTGGTTCAAGCTCGAGGCGTCAAGTAA

Upstream 100 bases:

>100_bases
GAACTTCGCTCGTGACCCTACTGTCAGCGTTTGGGGTTTTGATGTCGATCCATACACATCGTAAATGGATCGCTCAAGTT
TGATTAAGGTGAACTATTCA

Downstream 100 bases:

>100_bases
TGCGGGCATCGCCGATTCTTCAATCATTCAAGCTTCTGGCGCTGACCGGCCTGGCAGTGCTGGTTGCCAGTTGCTCGTCG
CCGTCACGCACAACGTCGTC

Product: membrane-bound lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MQAMRNWARYAPVVGLVGILGSVQEALAGDYDGSPQVAEFVGELTRDHGFAGEQLMSLFREVERKQAILDAISRPAERVK
QWKEYRPMFITDTRIARGVDFWRQHEAALARAEQEYGVPAQIIVSIIGVETFFGRNTGNYRVIDALSTLGFDYPPRAQFF
RKELREFLLLAREQQVDPLTLKGSYAGAMGLPQFMPSSFRAYAVDFDGDGHINIWTDPDDAIGSVASYFKRHGWVAGQPV
VSRATARGDRVDEGLSPALDPVMSVGELRQLGWASHDALRDDMPVTAFRLEGDNGPEYWLGLNNFYAITRYNRSVMYAMA
VYQLSESLVQARGVK

Sequences:

>Translated_335_residues
MQAMRNWARYAPVVGLVGILGSVQEALAGDYDGSPQVAEFVGELTRDHGFAGEQLMSLFREVERKQAILDAISRPAERVK
QWKEYRPMFITDTRIARGVDFWRQHEAALARAEQEYGVPAQIIVSIIGVETFFGRNTGNYRVIDALSTLGFDYPPRAQFF
RKELREFLLLAREQQVDPLTLKGSYAGAMGLPQFMPSSFRAYAVDFDGDGHINIWTDPDDAIGSVASYFKRHGWVAGQPV
VSRATARGDRVDEGLSPALDPVMSVGELRQLGWASHDALRDDMPVTAFRLEGDNGPEYWLGLNNFYAITRYNRSVMYAMA
VYQLSESLVQARGVK
>Mature_335_residues
MQAMRNWARYAPVVGLVGILGSVQEALAGDYDGSPQVAEFVGELTRDHGFAGEQLMSLFREVERKQAILDAISRPAERVK
QWKEYRPMFITDTRIARGVDFWRQHEAALARAEQEYGVPAQIIVSIIGVETFFGRNTGNYRVIDALSTLGFDYPPRAQFF
RKELREFLLLAREQQVDPLTLKGSYAGAMGLPQFMPSSFRAYAVDFDGDGHINIWTDPDDAIGSVASYFKRHGWVAGQPV
VSRATARGDRVDEGLSPALDPVMSVGELRQLGWASHDALRDDMPVTAFRLEGDNGPEYWLGLNNFYAITRYNRSVMYAMA
VYQLSESLVQARGVK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=315, Percent_Identity=46.3492063492063, Blast_Score=279, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 37558; Mature: 37558

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAMRNWARYAPVVGLVGILGSVQEALAGDYDGSPQVAEFVGELTRDHGFAGEQLMSLFR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
EVERKQAILDAISRPAERVKQWKEYRPMFITDTRIARGVDFWRQHEAALARAEQEYGVPA
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCH
QIIVSIIGVETFFGRNTGNYRVIDALSTLGFDYPPRAQFFRKELREFLLLAREQQVDPLT
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
LKGSYAGAMGLPQFMPSSFRAYAVDFDGDGHINIWTDPDDAIGSVASYFKRHGWVAGQPV
ECCCCCCCCCCHHHCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCHH
VSRATARGDRVDEGLSPALDPVMSVGELRQLGWASHDALRDDMPVTAFRLEGDNGPEYWL
HHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEEECCCCCCEEE
GLNNFYAITRYNRSVMYAMAVYQLSESLVQARGVK
ECCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQAMRNWARYAPVVGLVGILGSVQEALAGDYDGSPQVAEFVGELTRDHGFAGEQLMSLFR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
EVERKQAILDAISRPAERVKQWKEYRPMFITDTRIARGVDFWRQHEAALARAEQEYGVPA
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCH
QIIVSIIGVETFFGRNTGNYRVIDALSTLGFDYPPRAQFFRKELREFLLLAREQQVDPLT
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
LKGSYAGAMGLPQFMPSSFRAYAVDFDGDGHINIWTDPDDAIGSVASYFKRHGWVAGQPV
ECCCCCCCCCCHHHCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCHH
VSRATARGDRVDEGLSPALDPVMSVGELRQLGWASHDALRDDMPVTAFRLEGDNGPEYWL
HHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEEECCCCCCEEE
GLNNFYAITRYNRSVMYAMAVYQLSESLVQARGVK
ECCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]