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The map label for this gene is miaB [H]

Identifier: 66047572

GI number: 66047572

Start: 5198298

End: 5199626

Strand: Direct

Name: miaB [H]

Synonym: Psyr_4345

Alternate gene names: 66047572

Gene position: 5198298-5199626 (Clockwise)

Preceding gene: 66047570

Following gene: 66047573

Centisome position: 85.31

GC content: 61.02

Gene sequence:

>1329_bases
ATGGCCAAAAAGCTTTATATCGAAACCCACGGTTGCCAGATGAACGAGTACGACAGCTCGCGCATGGTCGACCTGCTGGG
CGAACATCAGGCCCTGGAAGTCACCGCTCGCGCGGAAGACGCCGACGTGATCCTGCTCAATACCTGCTCGATTCGTGAGC
GCGCCCAGGACCGGGTGTATTCCCAGCTCGGCCGCTGGCGCGAGCTGAAGCTGGCCAATCCGGAAATGGTCATCGCCGTC
GGTGGCTGCGTGGCCAGCCAGGAAGGCGCAGCCATTCGCGATCGCGCACCCTATGTGGACGTGGTGTTCGGCCCGCAGAC
CCTGCACCGCCTGCCGGAAATGATCGACGCCGCACGCGTGACCCGCTTGCCGCAGGTCGACGTCTCGTTCCCGGAAATCG
AAAAGTTCGACCATTTGCCCGAGCCACGCGTCGATGGCCCGAGTGCTTATGTGTCGGTGATGGAAGGCTGCAGCAAATAC
TGCACCTTCTGCGTGGTGCCCTACACCCGTGGCGAGGAAGTCAGTCGTCCGTTCGACGACGTGCTGAGTGAAGTCATCCA
CCTGGCCGAAAACGGCGTGCGTGAAGTCACTCTGCTGGGGCAGAACGTCAACGGTTATCGCGGCACCACCCACGACGGCC
GCGTTGCAGACCTGGCGGACCTGATCCGCGTAGTCGCCGCCGTCGATGGCATCGACCGCATTCGCTACACCACCTCGCAC
CCGCTGGAATTTTCCGACAGCCTGATCCAGGCCCACGCGGAAGTGCCGGAGCTGGTCAAACACCTGCATTTGCCTGTGCA
ATCGGGTTCCGACCGCATCCTGGCGGCGATGAAGCGCAACCACACCACGCTGGAATACAAGTCCCGCTTGCGCAAGCTCA
GGGCCGCAGTGCCGGGCATCAGTATCAGTTCCGACTTCATCGTCGGCTTCCCCGGCGAGACCGAAAAGGATTTCGACAAC
ACCATGAAGTTGATCGAGGACGTGGGTTTCGACTTCTCGTTCTCGTTCGTCTACAGCCCGCGTCCGGGCACGCCCGCCGC
GGACCTCAAGGACGACACGCCGGAAGCCCTGAAAAAAGAGCGACTGGCCGCGCTGCAACATCGTCTGAACCAGCAGGGTT
TCGAGATCAGCCGACAGATGGTCGGCAGCATCCAGCGCATTCTGGTAACCGACTACTCGAAGAAAGACCCGGGAGAACTG
CAGGGCCGCACCGAGAACAACCGGATTGTCAATTTTCGCTGCGACAACCCCAAATTGATCGGGCAGTTTGCCGATGTACA
CATCGATGATGCACAGCCTCACTCGTTGCGCGGCTCGCTGCTACAGTAA

Upstream 100 bases:

>100_bases
ACTATAGCATGTCCGCCCGCCTCGTCGGACAGCCAACGCTAGGAACCCGAGCCCTTCATCCTTATAATGCGCGGTTCACT
GACACGGTTGATCATCACGC

Downstream 100 bases:

>100_bases
AGCCTGAGTGTCGGCGCAGAAACGAATCGCCGAATGAATTCGTTTCTGCGCGGTCCCTACCATTGAGCACGGTGAATCCC
TGGCGCGGCCTTTAACAAGG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MAKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVYSQLGRWRELKLANPEMVIAV
GGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARVTRLPQVDVSFPEIEKFDHLPEPRVDGPSAYVSVMEGCSKY
CTFCVVPYTRGEEVSRPFDDVLSEVIHLAENGVREVTLLGQNVNGYRGTTHDGRVADLADLIRVVAAVDGIDRIRYTTSH
PLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTTLEYKSRLRKLRAAVPGISISSDFIVGFPGETEKDFDN
TMKLIEDVGFDFSFSFVYSPRPGTPAADLKDDTPEALKKERLAALQHRLNQQGFEISRQMVGSIQRILVTDYSKKDPGEL
QGRTENNRIVNFRCDNPKLIGQFADVHIDDAQPHSLRGSLLQ

Sequences:

>Translated_442_residues
MAKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVYSQLGRWRELKLANPEMVIAV
GGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARVTRLPQVDVSFPEIEKFDHLPEPRVDGPSAYVSVMEGCSKY
CTFCVVPYTRGEEVSRPFDDVLSEVIHLAENGVREVTLLGQNVNGYRGTTHDGRVADLADLIRVVAAVDGIDRIRYTTSH
PLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTTLEYKSRLRKLRAAVPGISISSDFIVGFPGETEKDFDN
TMKLIEDVGFDFSFSFVYSPRPGTPAADLKDDTPEALKKERLAALQHRLNQQGFEISRQMVGSIQRILVTDYSKKDPGEL
QGRTENNRIVNFRCDNPKLIGQFADVHIDDAQPHSLRGSLLQ
>Mature_441_residues
AKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVYSQLGRWRELKLANPEMVIAVG
GCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARVTRLPQVDVSFPEIEKFDHLPEPRVDGPSAYVSVMEGCSKYC
TFCVVPYTRGEEVSRPFDDVLSEVIHLAENGVREVTLLGQNVNGYRGTTHDGRVADLADLIRVVAAVDGIDRIRYTTSHP
LEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTTLEYKSRLRKLRAAVPGISISSDFIVGFPGETEKDFDNT
MKLIEDVGFDFSFSFVYSPRPGTPAADLKDDTPEALKKERLAALQHRLNQQGFEISRQMVGSIQRILVTDYSKKDPGELQ
GRTENNRIVNFRCDNPKLIGQFADVHIDDAQPHSLRGSLLQ

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=36.4016736401674, Blast_Score=278, Evalue=6e-75,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=36.4016736401674, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI93277076, Length=456, Percent_Identity=28.0701754385965, Blast_Score=168, Evalue=8e-42,
Organism=Escherichia coli, GI1786882, Length=442, Percent_Identity=65.158371040724, Blast_Score=613, Evalue=1e-177,
Organism=Escherichia coli, GI1787057, Length=453, Percent_Identity=27.8145695364238, Blast_Score=159, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17553146, Length=463, Percent_Identity=34.7732181425486, Blast_Score=254, Evalue=9e-68,
Organism=Caenorhabditis elegans, GI71996771, Length=262, Percent_Identity=33.206106870229, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356207, Length=484, Percent_Identity=32.6446280991736, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=28.4140969162996, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49452; Mature: 49320

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVY
CCCEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHH
SQLGRWRELKLANPEMVIAVGGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARV
HHHCCCEEEEECCCCEEEEECCEEECCCCCEECCCCCEEEEEECHHHHHHHHHHHHHHHH
TRLPQVDVSFPEIEKFDHLPEPRVDGPSAYVSVMEGCSKYCTFCVVPYTRGEEVSRPFDD
HCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCHHH
VLSEVIHLAENGVREVTLLGQNVNGYRGTTHDGRVADLADLIRVVAAVDGIDRIRYTTSH
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHEECCCC
PLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTTLEYKSRLRKLRAAVPGI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
SISSDFIVGFPGETEKDFDNTMKLIEDVGFDFSFSFVYSPRPGTPAADLKDDTPEALKKE
EECCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHH
RLAALQHRLNQQGFEISRQMVGSIQRILVTDYSKKDPGELQGRTENNRIVNFRCDNPKLI
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCEE
GQFADVHIDDAQPHSLRGSLLQ
EHHHCEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
AKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVY
CCEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHH
SQLGRWRELKLANPEMVIAVGGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARV
HHHCCCEEEEECCCCEEEEECCEEECCCCCEECCCCCEEEEEECHHHHHHHHHHHHHHHH
TRLPQVDVSFPEIEKFDHLPEPRVDGPSAYVSVMEGCSKYCTFCVVPYTRGEEVSRPFDD
HCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCHHH
VLSEVIHLAENGVREVTLLGQNVNGYRGTTHDGRVADLADLIRVVAAVDGIDRIRYTTSH
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHEECCCC
PLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTTLEYKSRLRKLRAAVPGI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
SISSDFIVGFPGETEKDFDNTMKLIEDVGFDFSFSFVYSPRPGTPAADLKDDTPEALKKE
EECCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHH
RLAALQHRLNQQGFEISRQMVGSIQRILVTDYSKKDPGELQGRTENNRIVNFRCDNPKLI
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCEE
GQFADVHIDDAQPHSLRGSLLQ
EHHHCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA