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The map label for this gene is gacS [H]

Identifier: 66047566

GI number: 66047566

Start: 5191591

End: 5193624

Strand: Reverse

Name: gacS [H]

Synonym: Psyr_4339

Alternate gene names: 66047566

Gene position: 5193624-5191591 (Counterclockwise)

Preceding gene: 66047571

Following gene: 66047565

Centisome position: 85.23

GC content: 59.69

Gene sequence:

>2034_bases
ATGGATGGATTGAACGCGACGACCTACCTTGGGGCTTCAATGCGCTATCTGTTGATCATTCTGGCTCTTTGGCTGCCGTT
GCAGAGTCATGCAGTCGAGTTTGATGAAAACACTCGTTTTCTGCCGCTGGGCCGTGCCATTCAGGTATTCGAGGACCCCA
CCGGCGAGGCGACCATCGAAAGCGTCAGCGCTGCCGCTCATGCCAGTGCCTTTAAGCCCGTGCCTCCCGGAACCTTCAAC
GCCGGCTATTCGCGCTCTGCCTTCTGGCTGAAGGTCGAGCTGACCTATCGCCCCACAGATGCCAGCATCCATAACGACTG
GCTGCTGGAACTGGCCTATCCGCCAATGGACCACATTGACTTCTACGGCCCGGATGCCGACGGGCAGCCCACTCGCGCCT
GGCATACCGGCGATATGCTGCCGTTCTCCAGCCGTCAGTTCGCCCAGAACAACTACCTCTTCCAGCTGGACCTGCCGCCT
GGCCAGACGCGAACCCTGTACATGCGTATTCGCAGCGAAGGCGCTGTTCAGGCACCGCTCTACCTCTGGTCCACCCACGC
CTATATGGATGCGCAGCCCACCAAGATTTACGTCTTCGGTCTGATCTACGGCGTGCTGCTGGGCATGCTGGTCTACAACC
TGTTTATCTACCTCAGCGTTCGTGAGGTGGATTACCTGTATTACCTGCTGTATGTCGCCTCGTTCGGGCTTTACCAGATG
TCGATCAACGGCGTTGCGATCGAATACCTATGGCCTGACAGCCCGTGGTGGGCGAATGCGTCGACCCCCTTCCTGATATC
GCTGGCGACGTTATTTGCGTGCCAGTTTTCGCGCAGCTTCCTGGGCACGGCACAACTGAGCCGCTGGCTGGACCGGCTGT
TGCTGGCGGTCATCGGCGCTGCCGTTCTGGTCATGGGTATGGCCTTGTTCCTCAGCTACGGGCCAGCCTTGCGCGGCGCC
GCTCAACTGGTCATGGCAGGCGCCCTGACGATCTACCTGGCGGGTATCGTTGCGGTCATCAAAGGCGAGCGGGTCGGGCG
CTATTTTGTGCTCGCCTGGTCGGTGTTCATGGTCAGCGGACTGATCTTCGGCTTGATGCTGCTTGGCTATCTGCCTAATA
CCTTCCTGACCATGTACGCCTCGCACATCGGTACGGTGCTGGAAATGGCGTTTCTGTCGATGGCGCTGGCCGACCGTATC
AACCACGCCCGCAGGCAGCAGGCGCAAACCTTGCTGGCTGCCGGTCAGGATCTGGAGCGTCTCAACCAGCAACTGGCCAA
CAGCAACCGGCTTAAGGATGAGTTTCTGGCCACGCTCACCCATGAGCTGCGCACGCCGATGAACGGCGTGATCGGGTCGC
TGGAAGTCATGCAGACGCTCGACATGGACGACGAAGTCGAGATGTATCAGCAAACCGCCGCCAGTTCGGCACGCGACATG
ATGAGCATGATCAACGGCATCCTGACCCTCACCGAGCTGCAGGCCGGGGTGCTGTATGCCGACTGCGAGGCGTTTTCGCC
CAAGGATCTGGCCGACAGGCTGCGTGAACGCTTCAGCGGTGCGGCTCAGTCCAAGGGCCTGATATTGACACTTGATCTGG
ACGATAAACTGCCGCCGAGCCTTCGTGGCGACGCGGGCAAGCTCTACCAGTGCATCGAGTGCCTGGTGGACAACGCGATC
AAATTCACCCGAAAAGGCGCAGTGCAGGTGCGTTTCAGTGGTCATCCGCAAGACCTGGAGCGCCTGTACCTGCGGGTCGA
AGTGATGGACAGCGGTATCGGTTTGAGCTGCCTTGACGAAGCCGGGCTGTATCAGCATTTCTTTCAGGTCGACGGCTCCA
TGACCCGTGAGTACGGTGGGCTGGGCATCGGTCTGGCGATCTGCCGGAAACTGATCGAGTTGCAGGGCGGCGAGCTGAGT
CATCGTTCCGAACCCGGCAAAGGCAGCTGTTTTACGCTGAGTGTCCCGATGCTGATGGTCGTACCGGGTGCAGTGCGCAG
GGCGCCTGAACTCAAGGCTCAATGGGGCATATAA

Upstream 100 bases:

>100_bases
CGGGCTTTTACTGATGTCTGCCGGTATATGTGCACGTTCAATGGTGTGTTTGAATCATTAGCCTGTTTCAAGGATAGAAA
TTTGCGGGACGTCGTAATCA

Downstream 100 bases:

>100_bases
GCGGCCTTATAGCGCCATCAGCTTAAAGCGCCGACCGTTAGGCGGCTGCGGTACTTTTGCGAGGGCCGGGTGCGTGATTC
ACTGTGTGCATATTGCGTGT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MDGLNATTYLGASMRYLLIILALWLPLQSHAVEFDENTRFLPLGRAIQVFEDPTGEATIESVSAAAHASAFKPVPPGTFN
AGYSRSAFWLKVELTYRPTDASIHNDWLLELAYPPMDHIDFYGPDADGQPTRAWHTGDMLPFSSRQFAQNNYLFQLDLPP
GQTRTLYMRIRSEGAVQAPLYLWSTHAYMDAQPTKIYVFGLIYGVLLGMLVYNLFIYLSVREVDYLYYLLYVASFGLYQM
SINGVAIEYLWPDSPWWANASTPFLISLATLFACQFSRSFLGTAQLSRWLDRLLLAVIGAAVLVMGMALFLSYGPALRGA
AQLVMAGALTIYLAGIVAVIKGERVGRYFVLAWSVFMVSGLIFGLMLLGYLPNTFLTMYASHIGTVLEMAFLSMALADRI
NHARRQQAQTLLAAGQDLERLNQQLANSNRLKDEFLATLTHELRTPMNGVIGSLEVMQTLDMDDEVEMYQQTAASSARDM
MSMINGILTLTELQAGVLYADCEAFSPKDLADRLRERFSGAAQSKGLILTLDLDDKLPPSLRGDAGKLYQCIECLVDNAI
KFTRKGAVQVRFSGHPQDLERLYLRVEVMDSGIGLSCLDEAGLYQHFFQVDGSMTREYGGLGIGLAICRKLIELQGGELS
HRSEPGKGSCFTLSVPMLMVVPGAVRRAPELKAQWGI

Sequences:

>Translated_677_residues
MDGLNATTYLGASMRYLLIILALWLPLQSHAVEFDENTRFLPLGRAIQVFEDPTGEATIESVSAAAHASAFKPVPPGTFN
AGYSRSAFWLKVELTYRPTDASIHNDWLLELAYPPMDHIDFYGPDADGQPTRAWHTGDMLPFSSRQFAQNNYLFQLDLPP
GQTRTLYMRIRSEGAVQAPLYLWSTHAYMDAQPTKIYVFGLIYGVLLGMLVYNLFIYLSVREVDYLYYLLYVASFGLYQM
SINGVAIEYLWPDSPWWANASTPFLISLATLFACQFSRSFLGTAQLSRWLDRLLLAVIGAAVLVMGMALFLSYGPALRGA
AQLVMAGALTIYLAGIVAVIKGERVGRYFVLAWSVFMVSGLIFGLMLLGYLPNTFLTMYASHIGTVLEMAFLSMALADRI
NHARRQQAQTLLAAGQDLERLNQQLANSNRLKDEFLATLTHELRTPMNGVIGSLEVMQTLDMDDEVEMYQQTAASSARDM
MSMINGILTLTELQAGVLYADCEAFSPKDLADRLRERFSGAAQSKGLILTLDLDDKLPPSLRGDAGKLYQCIECLVDNAI
KFTRKGAVQVRFSGHPQDLERLYLRVEVMDSGIGLSCLDEAGLYQHFFQVDGSMTREYGGLGIGLAICRKLIELQGGELS
HRSEPGKGSCFTLSVPMLMVVPGAVRRAPELKAQWGI
>Mature_677_residues
MDGLNATTYLGASMRYLLIILALWLPLQSHAVEFDENTRFLPLGRAIQVFEDPTGEATIESVSAAAHASAFKPVPPGTFN
AGYSRSAFWLKVELTYRPTDASIHNDWLLELAYPPMDHIDFYGPDADGQPTRAWHTGDMLPFSSRQFAQNNYLFQLDLPP
GQTRTLYMRIRSEGAVQAPLYLWSTHAYMDAQPTKIYVFGLIYGVLLGMLVYNLFIYLSVREVDYLYYLLYVASFGLYQM
SINGVAIEYLWPDSPWWANASTPFLISLATLFACQFSRSFLGTAQLSRWLDRLLLAVIGAAVLVMGMALFLSYGPALRGA
AQLVMAGALTIYLAGIVAVIKGERVGRYFVLAWSVFMVSGLIFGLMLLGYLPNTFLTMYASHIGTVLEMAFLSMALADRI
NHARRQQAQTLLAAGQDLERLNQQLANSNRLKDEFLATLTHELRTPMNGVIGSLEVMQTLDMDDEVEMYQQTAASSARDM
MSMINGILTLTELQAGVLYADCEAFSPKDLADRLRERFSGAAQSKGLILTLDLDDKLPPSLRGDAGKLYQCIECLVDNAI
KFTRKGAVQVRFSGHPQDLERLYLRVEVMDSGIGLSCLDEAGLYQHFFQVDGSMTREYGGLGIGLAICRKLIELQGGELS
HRSEPGKGSCFTLSVPMLMVVPGAVRRAPELKAQWGI

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=247, Percent_Identity=32.7935222672065, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=33.6065573770492, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI48994928, Length=249, Percent_Identity=32.1285140562249, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI87081816, Length=313, Percent_Identity=30.3514376996805, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1788713, Length=231, Percent_Identity=31.1688311688312, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1786600, Length=259, Percent_Identity=28.1853281853282, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1788393, Length=249, Percent_Identity=28.5140562248996, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1786912, Length=277, Percent_Identity=27.0758122743682, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1790346, Length=253, Percent_Identity=24.5059288537549, Blast_Score=70, Evalue=6e-13,
Organism=Escherichia coli, GI1786783, Length=250, Percent_Identity=24, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 75143; Mature: 75143

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGLNATTYLGASMRYLLIILALWLPLQSHAVEFDENTRFLPLGRAIQVFEDPTGEATIE
CCCCCCHHHHHHHHHHHHHHHHHHHCHHHHCEEECCCCEEEECCCEEEEEECCCCCHHHH
SVSAAAHASAFKPVPPGTFNAGYSRSAFWLKVELTYRPTDASIHNDWLLELAYPPMDHID
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCC
FYGPDADGQPTRAWHTGDMLPFSSRQFAQNNYLFQLDLPPGQTRTLYMRIRSEGAVQAPL
CCCCCCCCCCCCEEECCCCCCCCCCHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCCE
YLWSTHAYMDAQPTKIYVFGLIYGVLLGMLVYNLFIYLSVREVDYLYYLLYVASFGLYQM
EEEECCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
SINGVAIEYLWPDSPWWANASTPFLISLATLFACQFSRSFLGTAQLSRWLDRLLLAVIGA
EECCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLVMGMALFLSYGPALRGAAQLVMAGALTIYLAGIVAVIKGERVGRYFVLAWSVFMVSG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LIFGLMLLGYLPNTFLTMYASHIGTVLEMAFLSMALADRINHARRQQAQTLLAAGQDLER
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LNQQLANSNRLKDEFLATLTHELRTPMNGVIGSLEVMQTLDMDDEVEMYQQTAASSARDM
HHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MSMINGILTLTELQAGVLYADCEAFSPKDLADRLRERFSGAAQSKGLILTLDLDDKLPPS
HHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
LRGDAGKLYQCIECLVDNAIKFTRKGAVQVRFSGHPQDLERLYLRVEVMDSGIGLSCLDE
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHEEEEECCCCCCHHHHH
AGLYQHFFQVDGSMTREYGGLGIGLAICRKLIELQGGELSHRSEPGKGSCFTLSVPMLMV
HHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHH
VPGAVRRAPELKAQWGI
CCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDGLNATTYLGASMRYLLIILALWLPLQSHAVEFDENTRFLPLGRAIQVFEDPTGEATIE
CCCCCCHHHHHHHHHHHHHHHHHHHCHHHHCEEECCCCEEEECCCEEEEEECCCCCHHHH
SVSAAAHASAFKPVPPGTFNAGYSRSAFWLKVELTYRPTDASIHNDWLLELAYPPMDHID
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCC
FYGPDADGQPTRAWHTGDMLPFSSRQFAQNNYLFQLDLPPGQTRTLYMRIRSEGAVQAPL
CCCCCCCCCCCCEEECCCCCCCCCCHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCCE
YLWSTHAYMDAQPTKIYVFGLIYGVLLGMLVYNLFIYLSVREVDYLYYLLYVASFGLYQM
EEEECCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
SINGVAIEYLWPDSPWWANASTPFLISLATLFACQFSRSFLGTAQLSRWLDRLLLAVIGA
EECCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLVMGMALFLSYGPALRGAAQLVMAGALTIYLAGIVAVIKGERVGRYFVLAWSVFMVSG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LIFGLMLLGYLPNTFLTMYASHIGTVLEMAFLSMALADRINHARRQQAQTLLAAGQDLER
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LNQQLANSNRLKDEFLATLTHELRTPMNGVIGSLEVMQTLDMDDEVEMYQQTAASSARDM
HHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MSMINGILTLTELQAGVLYADCEAFSPKDLADRLRERFSGAAQSKGLILTLDLDDKLPPS
HHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
LRGDAGKLYQCIECLVDNAIKFTRKGAVQVRFSGHPQDLERLYLRVEVMDSGIGLSCLDE
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHEEEEECCCCCCHHHHH
AGLYQHFFQVDGSMTREYGGLGIGLAICRKLIELQGGELSHRSEPGKGSCFTLSVPMLMV
HHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHH
VPGAVRRAPELKAQWGI
CCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]