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The map label for this gene is mccA [H]

Identifier: 66047548

GI number: 66047548

Start: 5173582

End: 5174484

Strand: Reverse

Name: mccA [H]

Synonym: Psyr_4321

Alternate gene names: 66047548

Gene position: 5174484-5173582 (Counterclockwise)

Preceding gene: 66047549

Following gene: 66047547

Centisome position: 84.92

GC content: 48.06

Gene sequence:

>903_bases
ATGAGTGTGCTTATCAGAACTCATGATCGAGTTTTAACCAAGCTGGAAATGAATAGCCCAGGAGGTAGTCACAAATATCG
CGCAGCTAATTTAATTGTAAAAAATGCGCTACGCAATGGGAAAATTATTCCTGGTATAACTACTGTAATTGAAAAGGCGG
GTGGCAATTTTGGTTTTGGTCTGTTATCTGCCTGCCACGCGTATGGTATTCCGGTTGAACTGGCAGTTGGCCTCGGTTTT
AGTCAGACCAAAAGAGATTTGTTGGAATGCTTTGGTGCCAAATTGATTGGTAAGGATATGTTGATCGCAGGCGCATCGCC
GAGGGATGTCGTTGCCCATCATTTGGAGCATCAGGTCGAAATGGACAAGTGTTACTACTATACCGATCAGTTTAATAACC
AAATCGGGATCGAGGCATATCGCTACCAGACTGCATTTGAGCTTGCCTGCCAATTAGAAGAAGCTGAGGCCGACAAAGAG
CTTTTGTTTATAGGCTGCGCAGGGACGGGGGCGAGCTTTACCGGTATTACCTTGGGCTTGAAGGACAATGGTTTCGATGT
ATCTACTGTCTTGGTCGACCCCTCGGGTTGCGACTCCAAGAATGGTGTTTTTTCAGACCATCGTTTTGAAGGGATGGCGG
TTGGCGTCTGCCCACCCTTTCTGGACTGGTCCTTGGTAAACAGATGCTATCAAGTCGAACATGGCGAGATGCTGGCAGCG
CAACGCGGATTCTATATTCAGAGCGGTACTTTTGTCGGCAATACCGCAGCGGCATGTCTGGCAGTCGCTCAGCGTCTTGC
CTGCCTTCCGGAATACGCAGAGACCACGTTGGTGACAATCGCTTATGACAACGGGCTCTGGTATCAGGATTTACAAAGGT
CAGTGGCATTAAGTGCAGCTTAG

Upstream 100 bases:

>100_bases
ACCAACGCTATTTGTATTGGGCTATGCATCTTTTTGTTAGCTGTAATTGTTGCGCTGTTGGATCAAAAAAAACGGGCCAC
GCCATGGAGGGAGGCATTAA

Downstream 100 bases:

>100_bases
TGACGTTTATGTAAGGCTAGAAAATCGTCTAGGGTTATGGGGTGTCAAATTTTTAAGAAGGGTGAGTAGAGAGAGGGCTT
TCTAAAGCGTTATGCCAATA

Product: pyridoxal-5'-phosphate-dependent enzyme, beta subunit

Products: @CYST01txt*L-cysteine!; acetate; Coenzyme A [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSVLIRTHDRVLTKLEMNSPGGSHKYRAANLIVKNALRNGKIIPGITTVIEKAGGNFGFGLLSACHAYGIPVELAVGLGF
SQTKRDLLECFGAKLIGKDMLIAGASPRDVVAHHLEHQVEMDKCYYYTDQFNNQIGIEAYRYQTAFELACQLEEAEADKE
LLFIGCAGTGASFTGITLGLKDNGFDVSTVLVDPSGCDSKNGVFSDHRFEGMAVGVCPPFLDWSLVNRCYQVEHGEMLAA
QRGFYIQSGTFVGNTAAACLAVAQRLACLPEYAETTLVTIAYDNGLWYQDLQRSVALSAA

Sequences:

>Translated_300_residues
MSVLIRTHDRVLTKLEMNSPGGSHKYRAANLIVKNALRNGKIIPGITTVIEKAGGNFGFGLLSACHAYGIPVELAVGLGF
SQTKRDLLECFGAKLIGKDMLIAGASPRDVVAHHLEHQVEMDKCYYYTDQFNNQIGIEAYRYQTAFELACQLEEAEADKE
LLFIGCAGTGASFTGITLGLKDNGFDVSTVLVDPSGCDSKNGVFSDHRFEGMAVGVCPPFLDWSLVNRCYQVEHGEMLAA
QRGFYIQSGTFVGNTAAACLAVAQRLACLPEYAETTLVTIAYDNGLWYQDLQRSVALSAA
>Mature_299_residues
SVLIRTHDRVLTKLEMNSPGGSHKYRAANLIVKNALRNGKIIPGITTVIEKAGGNFGFGLLSACHAYGIPVELAVGLGFS
QTKRDLLECFGAKLIGKDMLIAGASPRDVVAHHLEHQVEMDKCYYYTDQFNNQIGIEAYRYQTAFELACQLEEAEADKEL
LFIGCAGTGASFTGITLGLKDNGFDVSTVLVDPSGCDSKNGVFSDHRFEGMAVGVCPPFLDWSLVNRCYQVEHGEMLAAQ
RGFYIQSGTFVGNTAAACLAVAQRLACLPEYAETTLVTIAYDNGLWYQDLQRSVALSAA

Specific function: Catalyzes the conversion of O-acetylserine and homocysteine to cystathionine [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=263, Percent_Identity=26.615969581749, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI295821200, Length=263, Percent_Identity=26.615969581749, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4557415, Length=263, Percent_Identity=26.615969581749, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1788754, Length=295, Percent_Identity=25.7627118644068, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367138, Length=280, Percent_Identity=27.1428571428571, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535051, Length=282, Percent_Identity=26.9503546099291, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI32566674, Length=271, Percent_Identity=25.830258302583, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17562970, Length=271, Percent_Identity=25.830258302583, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI115535073, Length=290, Percent_Identity=24.4827586206897, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17561720, Length=271, Percent_Identity=25.830258302583, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=289, Percent_Identity=28.0276816608997, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24643623, Length=185, Percent_Identity=31.3513513513514, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI20129101, Length=185, Percent_Identity=31.3513513513514, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 2.5.1.47 [C]

Molecular weight: Translated: 32539; Mature: 32408

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLIRTHDRVLTKLEMNSPGGSHKYRAANLIVKNALRNGKIIPGITTVIEKAGGNFGFG
CEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHH
LLSACHAYGIPVELAVGLGFSQTKRDLLECFGAKLIGKDMLIAGASPRDVVAHHLEHQVE
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCHHHCCCCEEEECCCHHHHHHHHHHHHHC
MDKCYYYTDQFNNQIGIEAYRYQTAFELACQLEEAEADKELLFIGCAGTGASFTGITLGL
HHHEEEEECCCCCEECCEEEEHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEE
KDNGFDVSTVLVDPSGCDSKNGVFSDHRFEGMAVGVCPPFLDWSLVNRCYQVEHGEMLAA
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEC
QRGFYIQSGTFVGNTAAACLAVAQRLACLPEYAETTLVTIAYDNGLWYQDLQRSVALSAA
CCCEEEECCCEECHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SVLIRTHDRVLTKLEMNSPGGSHKYRAANLIVKNALRNGKIIPGITTVIEKAGGNFGFG
EEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHH
LLSACHAYGIPVELAVGLGFSQTKRDLLECFGAKLIGKDMLIAGASPRDVVAHHLEHQVE
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCHHHCCCCEEEECCCHHHHHHHHHHHHHC
MDKCYYYTDQFNNQIGIEAYRYQTAFELACQLEEAEADKELLFIGCAGTGASFTGITLGL
HHHEEEEECCCCCEECCEEEEHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEE
KDNGFDVSTVLVDPSGCDSKNGVFSDHRFEGMAVGVCPPFLDWSLVNRCYQVEHGEMLAA
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEC
QRGFYIQSGTFVGNTAAACLAVAQRLACLPEYAETTLVTIAYDNGLWYQDLQRSVALSAA
CCCEEEECCCEECHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 44000 [C]

Specific activity: 1100

Km value (mM): 0.12 {acetyl-CoA}} 3.6 {S2-}} 0.1 {S2-}} 20 {O-acetyl-L-Ser}} 7.1 {O-acetyl-L-Ser}} 5 {O-acetyl-L-Ser}} 0.7 {L-Ser}} [C]

Substrates: O-acetyl-L-serine; L-serine; acetyl-CoA; H2S [C]

Specific reaction: O-acetyl-L-serine + H2S = @CYST01.txt*L-cysteine! + acetate L-serine + acetyl-CoA + H2S = L-cysteine + Coenzyme A + acetate [C]

General reaction: Elimination; C-O bond cleavage [C]

Inhibitor: acetyl-CoA; D-cycloserine; L-cysteine; NH2OH; semicarbazide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]