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The map label for this gene is asnB [H]

Identifier: 66047542

GI number: 66047542

Start: 5164033

End: 5166009

Strand: Reverse

Name: asnB [H]

Synonym: Psyr_4315

Alternate gene names: 66047542

Gene position: 5166009-5164033 (Counterclockwise)

Preceding gene: 66047543

Following gene: 66047541

Centisome position: 84.78

GC content: 49.27

Gene sequence:

>1977_bases
ATGTGTGGGATAACTGGCTTCTTTGGTGTTAAAAATAAAAATCTCGATGAAACCACCGTCATAAAAAATATGCTTTCAAT
GATTGAACATCGAGGCCCCGACCAGGCAGGGTATTATGTCAATAATTCCATGGGTCTCGGCACCATGCGTTTAAGTATTA
TTGACCTTGCGGCAGGTATTCAACCCCTCAGCGATGGCACAGGTCAGTATTGGATTTCATACAACGGTGAACTGTATAAC
TACATCGAGCTAAGGCGTCGATTGCAGGTCTTGGGACACCATTTTTCCACTGAGTCCGACACCGAGGTGGTGTTGCGCAG
CTGGATGGAGTGGGGTGATGAGGCCTTGGAAAAATTTAATGGCGCTTTCGCCTTCGCCATTTATGACACGGTTAAAGATG
AAGTATTTATAGCGCGCGATCGCTTTGGTAAGCGCCCGCTGTTTTATACGCAAACCGACGGTGGTTATCTTTTTGCTTCA
GAGATGAAGTGCTTTTTTGGCTTTCCAGGGTTTGAGTTCAAGTTTGACGAAATGTCCTTGTCCTCAATCCTCGCCCATTG
GACACCCCTGCCATCGCAAACCCCGTTTCAGGGCATTCGTCAGCTGCCACCTGGGTCCATCATGACCTTGCGAGGGGCTC
AGTGTGAGATCCGGCCCTACTACGCCCTGGATTTTCGCGCTGTGGATTTTGATGGCACTGAAAATGAAGCCAAGGAAGAA
ATCCGCAACACCCTCGAGCAGGCGGTCAAGCTCAGACTGCGCAGTGACGTGCCTATGGGGCTGTACCTCAGTGGTGGTGT
TGACTCCTCCGTTTTGACCAGTATTGCGGCAAGGATCATCAAGCAAAAGCCGCTGCGCACGTTTTCTGTGGAGTTTGAAG
ATAAGAACCTGGATGAGTCGAGTGATCAACAACTCGTCTCTGCGTTGTTCTCGACCAAGCATGAACGGTTGATTATTAAC
CATAAAGACATTGTTGATAATTTCTCGGCGACGGTTTTCCATGCTGAAATGCCGTTGTTTCGTACCGCGCCCGTGCCGAT
GTACATGTTGTCCAAGCATGTTCGCTCGACCGGTATCAAAGCGATTCTGAGCGGAGAGGGAGCGGATGAAGTATTTCTGG
GCTACAACATCTTTAAAGAGACTCTGTTGCGCTCCCAGTGGGACACCACTGATCACGATGAGAAGAAACGCTTGCTGGCC
AAGTTGTATCCTTACATGAAAAGCTTCAGTGAAGACAATGACTCGAACTTGATGGGTTTCTATAACGCTTATGCGAAAGA
AAAAATCCCTGGGTTGTTCTCCCACGAAATACGTTTTCAAAATGGGCGCTTTGCCAGTCGGTTGCTGAAAACCAAGAATG
ATCCATTTGCTGGCATTCAGGCATGGGTGGCGAAAGACCCGGTGTACAGCGCGTTAAGCCCAACGCAAAAAGCCCAGTGG
CTGGAGTTCAATACCTTGCTTTCCGGGTATCTCCTCAGTAGCCAGGGCGAGCGTATGGGCCTTGCTCATGGTATTGAAAA
CCGCTGTCCATTCCTCGACATCAATGTGCAGAAACTCGCCTCCTCCATCAATTTGCGTTTTGACGATGGCTTTACTGAAA
AGTACATGCTGCGCATGGCCTACGATGGAATTCTGCCGGGTCGTATCATTCATAAAGCCAAAACACCGTATCGTGCGCCG
GACAGCGGGCCGTTTGTCGAACACGCCCTCGATTATCTCAATCATCTTTGCGAGTCAAATGCGCTGGACGCCATCGAGCT
GATTAATCCGGTGTTTGCAAAAGCACTGATCAAAAAAGTGGTCGAACGAACCCTTGCCGGCGGTGAAGACATAACAGTCA
GAGACAACCAGGCGTTTGTCTATCTGATGTCCTTGATCGAGCTGGATAAATGGTTTGTCAAACGTCAGGGCGCGGCTTAT
ATCAATCCTTGCCGGGTGGATAAGTTGTTGGTGCAAGCCGTAGACGCAAGGCACTAG

Upstream 100 bases:

>100_bases
AAACTCCAAGCCGTGATTGGGACTTTCGACCTTCGCTAAGGCGGTAGGGCGCTACAGTTCTGTGTGGGGTGCCAACTTAA
ACTTTGGTGGGTTTATTTTT

Downstream 100 bases:

>100_bases
AAACCTAAGGGACAACGTTTATGTCAAGCACAAAAACTGACCTAGATAGCACCCAGGTCGCTATTGTCGGGGTTGGCCTT
CGCCTGCCTGAAGCAGACTC

Product: asparagine synthase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MCGITGFFGVKNKNLDETTVIKNMLSMIEHRGPDQAGYYVNNSMGLGTMRLSIIDLAAGIQPLSDGTGQYWISYNGELYN
YIELRRRLQVLGHHFSTESDTEVVLRSWMEWGDEALEKFNGAFAFAIYDTVKDEVFIARDRFGKRPLFYTQTDGGYLFAS
EMKCFFGFPGFEFKFDEMSLSSILAHWTPLPSQTPFQGIRQLPPGSIMTLRGAQCEIRPYYALDFRAVDFDGTENEAKEE
IRNTLEQAVKLRLRSDVPMGLYLSGGVDSSVLTSIAARIIKQKPLRTFSVEFEDKNLDESSDQQLVSALFSTKHERLIIN
HKDIVDNFSATVFHAEMPLFRTAPVPMYMLSKHVRSTGIKAILSGEGADEVFLGYNIFKETLLRSQWDTTDHDEKKRLLA
KLYPYMKSFSEDNDSNLMGFYNAYAKEKIPGLFSHEIRFQNGRFASRLLKTKNDPFAGIQAWVAKDPVYSALSPTQKAQW
LEFNTLLSGYLLSSQGERMGLAHGIENRCPFLDINVQKLASSINLRFDDGFTEKYMLRMAYDGILPGRIIHKAKTPYRAP
DSGPFVEHALDYLNHLCESNALDAIELINPVFAKALIKKVVERTLAGGEDITVRDNQAFVYLMSLIELDKWFVKRQGAAY
INPCRVDKLLVQAVDARH

Sequences:

>Translated_658_residues
MCGITGFFGVKNKNLDETTVIKNMLSMIEHRGPDQAGYYVNNSMGLGTMRLSIIDLAAGIQPLSDGTGQYWISYNGELYN
YIELRRRLQVLGHHFSTESDTEVVLRSWMEWGDEALEKFNGAFAFAIYDTVKDEVFIARDRFGKRPLFYTQTDGGYLFAS
EMKCFFGFPGFEFKFDEMSLSSILAHWTPLPSQTPFQGIRQLPPGSIMTLRGAQCEIRPYYALDFRAVDFDGTENEAKEE
IRNTLEQAVKLRLRSDVPMGLYLSGGVDSSVLTSIAARIIKQKPLRTFSVEFEDKNLDESSDQQLVSALFSTKHERLIIN
HKDIVDNFSATVFHAEMPLFRTAPVPMYMLSKHVRSTGIKAILSGEGADEVFLGYNIFKETLLRSQWDTTDHDEKKRLLA
KLYPYMKSFSEDNDSNLMGFYNAYAKEKIPGLFSHEIRFQNGRFASRLLKTKNDPFAGIQAWVAKDPVYSALSPTQKAQW
LEFNTLLSGYLLSSQGERMGLAHGIENRCPFLDINVQKLASSINLRFDDGFTEKYMLRMAYDGILPGRIIHKAKTPYRAP
DSGPFVEHALDYLNHLCESNALDAIELINPVFAKALIKKVVERTLAGGEDITVRDNQAFVYLMSLIELDKWFVKRQGAAY
INPCRVDKLLVQAVDARH
>Mature_658_residues
MCGITGFFGVKNKNLDETTVIKNMLSMIEHRGPDQAGYYVNNSMGLGTMRLSIIDLAAGIQPLSDGTGQYWISYNGELYN
YIELRRRLQVLGHHFSTESDTEVVLRSWMEWGDEALEKFNGAFAFAIYDTVKDEVFIARDRFGKRPLFYTQTDGGYLFAS
EMKCFFGFPGFEFKFDEMSLSSILAHWTPLPSQTPFQGIRQLPPGSIMTLRGAQCEIRPYYALDFRAVDFDGTENEAKEE
IRNTLEQAVKLRLRSDVPMGLYLSGGVDSSVLTSIAARIIKQKPLRTFSVEFEDKNLDESSDQQLVSALFSTKHERLIIN
HKDIVDNFSATVFHAEMPLFRTAPVPMYMLSKHVRSTGIKAILSGEGADEVFLGYNIFKETLLRSQWDTTDHDEKKRLLA
KLYPYMKSFSEDNDSNLMGFYNAYAKEKIPGLFSHEIRFQNGRFASRLLKTKNDPFAGIQAWVAKDPVYSALSPTQKAQW
LEFNTLLSGYLLSSQGERMGLAHGIENRCPFLDINVQKLASSINLRFDDGFTEKYMLRMAYDGILPGRIIHKAKTPYRAP
DSGPFVEHALDYLNHLCESNALDAIELINPVFAKALIKKVVERTLAGGEDITVRDNQAFVYLMSLIELDKWFVKRQGAAY
INPCRVDKLLVQAVDARH

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=413, Percent_Identity=29.7820823244552, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI168229252, Length=413, Percent_Identity=29.7820823244552, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI168229250, Length=413, Percent_Identity=29.7820823244552, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI296010848, Length=385, Percent_Identity=29.8701298701299, Blast_Score=139, Evalue=1e-32,
Organism=Homo sapiens, GI296010852, Length=309, Percent_Identity=29.7734627831715, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI296010850, Length=309, Percent_Identity=29.7734627831715, Blast_Score=105, Evalue=2e-22,
Organism=Escherichia coli, GI1786889, Length=422, Percent_Identity=33.175355450237, Blast_Score=192, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI71993933, Length=391, Percent_Identity=29.923273657289, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI25147557, Length=391, Percent_Identity=29.923273657289, Blast_Score=145, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17560178, Length=393, Percent_Identity=30.2798982188295, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25147560, Length=326, Percent_Identity=27.9141104294479, Blast_Score=107, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321563, Length=427, Percent_Identity=29.03981264637, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325403, Length=423, Percent_Identity=26.4775413711584, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45553209, Length=404, Percent_Identity=31.1881188118812, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 74704; Mature: 74704

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGFFGVKNKNLDETTVIKNMLSMIEHRGPDQAGYYVNNSMGLGTMRLSIIDLAAGI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHCC
QPLSDGTGQYWISYNGELYNYIELRRRLQVLGHHFSTESDTEVVLRSWMEWGDEALEKFN
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
GAFAFAIYDTVKDEVFIARDRFGKRPLFYTQTDGGYLFASEMKCFFGFPGFEFKFDEMSL
CEEEEEEEHHCCCCEEEEHHCCCCCCEEEEECCCCEEEEECCHHEECCCCCEEEECHHHH
SSILAHWTPLPSQTPFQGIRQLPPGSIMTLRGAQCEIRPYYALDFRAVDFDGTENEAKEE
HHHHHHCCCCCCCCCHHHHHCCCCCCEEEEECCCEEEEEEEEEEEEEECCCCCCHHHHHH
IRNTLEQAVKLRLRSDVPMGLYLSGGVDSSVLTSIAARIIKQKPLRTFSVEFEDKNLDES
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
SDQQLVSALFSTKHERLIINHKDIVDNFSATVFHAEMPLFRTAPVPMYMLSKHVRSTGIK
HHHHHHHHHHHCCCCEEEEECHHHHCCCCCEEEEECCCCEECCCCCHHHHHHHHHHHCCE
AILSGEGADEVFLGYNIFKETLLRSQWDTTDHDEKKRLLAKLYPYMKSFSEDNDSNLMGF
EEECCCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHH
YNAYAKEKIPGLFSHEIRFQNGRFASRLLKTKNDPFAGIQAWVAKDPVYSALSPTQKAQW
HHHHHHHHCCCCHHHCEEECCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHCCCCHHHHH
LEFNTLLSGYLLSSQGERMGLAHGIENRCPFLDINVQKLASSINLRFDDGFTEKYMLRMA
HHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEECCHHHHHHHCCEEECCCCHHHHHHHHH
YDGILPGRIIHKAKTPYRAPDSGPFVEHALDYLNHLCESNALDAIELINPVFAKALIKKV
HCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VERTLAGGEDITVRDNQAFVYLMSLIELDKWFVKRQGAAYINPCRVDKLLVQAVDARH
HHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGITGFFGVKNKNLDETTVIKNMLSMIEHRGPDQAGYYVNNSMGLGTMRLSIIDLAAGI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHCC
QPLSDGTGQYWISYNGELYNYIELRRRLQVLGHHFSTESDTEVVLRSWMEWGDEALEKFN
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
GAFAFAIYDTVKDEVFIARDRFGKRPLFYTQTDGGYLFASEMKCFFGFPGFEFKFDEMSL
CEEEEEEEHHCCCCEEEEHHCCCCCCEEEEECCCCEEEEECCHHEECCCCCEEEECHHHH
SSILAHWTPLPSQTPFQGIRQLPPGSIMTLRGAQCEIRPYYALDFRAVDFDGTENEAKEE
HHHHHHCCCCCCCCCHHHHHCCCCCCEEEEECCCEEEEEEEEEEEEEECCCCCCHHHHHH
IRNTLEQAVKLRLRSDVPMGLYLSGGVDSSVLTSIAARIIKQKPLRTFSVEFEDKNLDES
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
SDQQLVSALFSTKHERLIINHKDIVDNFSATVFHAEMPLFRTAPVPMYMLSKHVRSTGIK
HHHHHHHHHHHCCCCEEEEECHHHHCCCCCEEEEECCCCEECCCCCHHHHHHHHHHHCCE
AILSGEGADEVFLGYNIFKETLLRSQWDTTDHDEKKRLLAKLYPYMKSFSEDNDSNLMGF
EEECCCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHH
YNAYAKEKIPGLFSHEIRFQNGRFASRLLKTKNDPFAGIQAWVAKDPVYSALSPTQKAQW
HHHHHHHHCCCCHHHCEEECCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHCCCCHHHHH
LEFNTLLSGYLLSSQGERMGLAHGIENRCPFLDINVQKLASSINLRFDDGFTEKYMLRMA
HHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEECCHHHHHHHCCEEECCCCHHHHHHHHH
YDGILPGRIIHKAKTPYRAPDSGPFVEHALDYLNHLCESNALDAIELINPVFAKALIKKV
HCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VERTLAGGEDITVRDNQAFVYLMSLIELDKWFVKRQGAAYINPCRVDKLLVQAVDARH
HHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]