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The map label for this gene is radA [H]

Identifier: 66047502

GI number: 66047502

Start: 5099513

End: 5100880

Strand: Reverse

Name: radA [H]

Synonym: Psyr_4275

Alternate gene names: 66047502

Gene position: 5100880-5099513 (Counterclockwise)

Preceding gene: 66047504

Following gene: 66047500

Centisome position: 83.71

GC content: 64.11

Gene sequence:

>1368_bases
ATGGCCAAGGCCAAACGCTTGTACGGCTGCACCGAGTGCGGCGCGACATTTCCCAAGTGGGCGGGCCAGTGCAGCGAATG
CGGTGCCTGGAACACACTGGTCGAAACCATGATTGAAAGCGGTGCCGCCGCGCCGCCTACCGGGCGTACCGGCTGGACCG
GTTCCCAAGCGCAGATCAGGACCCTGGCGGAGGTCAGCGTCGAAGAAATCCCGCGCTTCTCCACCAACTCCACCGAGCTG
GACAGGGTACTCGGCGGCGGTCTGGTTGACGGTTCGGTGGTGCTGATTGGCGGCGACCCCGGCATCGGCAAGTCCACGAT
CCTCTTGCAGACACTGTGCAACATCGCCCAGCGCATGCCCGCGCTGTATGTCACCGGTGAGGAATCCCAGCAGCAGGTCG
CCATGCGCGCCCGGCGCCTGGGGCTGCCGCAGGACAAGCTGCGGGTCATGACCGAAACCTGCATCGAGACCATCATTGCC
ACCGCCAAGGTCGAGAAGCCCAAGGTGATGGTGATCGATTCGATCCAGACGATTTTCACCGAACAGCTGCAGTCGGCCCC
TGGCGGCGTGTCGCAGGTTCGCGAAAGCGCCGCCTTGCTGGTGCGCTACGCCAAGCAGAGCGGTACGGCGATCTTCCTGG
TCGGCCACGTCACCAAGGAAGGTGCACTGGCCGGCCCGCGTGTGCTTGAGCACATGGTCGACACCGTGTTGTATTTCGAG
GGCGAATCGGACGGCCGCCTGCGTTTGCTGCGCGCGGTGAAGAACCGCTTTGGCGCGGTCAACGAGCTGGGCGTGTTCGG
CATGACCGACAAGGGCCTGAAAGAAGTCTCCAACCCGTCGGCGATCTTTCTCACCCGTGCGCAGGAAGAAGTACCGGGCA
GTGTGGTCATGGCCACTTGGGAGGGCACGCGGCCGATGCTGGTCGAGGTGCAGGCGCTGGTGGACGACAGTCACATGTCC
AATCCGCGCCGCGTAACGCTGGGTCTGGATCAGAATCGTCTGGCCATGTTGCTGGCGGTCCTGCACCGACATGGCGGCAT
TCCCACTCACGATCAGGACGTGTTTCTCAACGTGGTGGGTGGTGTGAAGGTACTGGAAACCGCCTCCGACCTGGCCTTGA
TGGCAGCGGTGATGTCCAGCCTGCGCAATCGTCCGTTGCCGCATGACTTGCTGGTGTTCGGTGAGGTGGGGCTGTCGGGC
GAAGTCAGGCCGGTGCCCAGCGGCCAGGAGCGCCTCAAGGAGGCCGCCAAGCACGGCTTCAAGCGCGCCATCGTGCCCAA
GGGCAATGCGCCGAAGGAAGCGCCGCCCGGCTTGCAGATCATTGCCGTTACGCGCCTGGAGCAGGCCCTGGATGCGCTGT
TCGAGTAA

Upstream 100 bases:

>100_bases
GCGTAAGTTTTATCCGGTTTTCAAAAGCAGTTGTCGCCGAGCGACTGGCAGGGGTTGCAACCTGCGTTATCATGGGCGTT
TTTTAACGACTGGGGTTGTC

Downstream 100 bases:

>100_bases
GCAGGTCTAGAGTTCGATCAGTGCTGCCAGCTCGCGGTCCAGTTCGTCCTGATCGGCAAGGTTCAGCTCGATCAGGCGCC
GCAGATGGCTGATCGACTCC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MAKAKRLYGCTECGATFPKWAGQCSECGAWNTLVETMIESGAAAPPTGRTGWTGSQAQIRTLAEVSVEEIPRFSTNSTEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRMPALYVTGEESQQQVAMRARRLGLPQDKLRVMTETCIETIIA
TAKVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHMS
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Sequences:

>Translated_455_residues
MAKAKRLYGCTECGATFPKWAGQCSECGAWNTLVETMIESGAAAPPTGRTGWTGSQAQIRTLAEVSVEEIPRFSTNSTEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRMPALYVTGEESQQQVAMRARRLGLPQDKLRVMTETCIETIIA
TAKVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHMS
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE
>Mature_454_residues
AKAKRLYGCTECGATFPKWAGQCSECGAWNTLVETMIESGAAAPPTGRTGWTGSQAQIRTLAEVSVEEIPRFSTNSTELD
RVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRMPALYVTGEESQQQVAMRARRLGLPQDKLRVMTETCIETIIAT
AKVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFEG
ESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHMSN
PRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSGE
VRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=460, Percent_Identity=63.2608695652174, Blast_Score=601, Evalue=1e-173,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 48914; Mature: 48783

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKRLYGCTECGATFPKWAGQCSECGAWNTLVETMIESGAAAPPTGRTGWTGSQAQIR
CCCCHHHCCCHHHCCCCCHHCCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
TLAEVSVEEIPRFSTNSTELDRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRMP
HHHHHHHHHCCCCCCCCHHHHHHHCCCEECCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
ALYVTGEESQQQVAMRARRLGLPQDKLRVMTETCIETIIATAKVEKPKVMVIDSIQTIFT
EEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHH
EQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHEEEC
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATW
CCCCCHHHHHHHHHHHHCCHHHCCEECCCCHHHHHCCCCCEEEEEECHHHCCCCEEEEEE
EGTRPMLVEVQALVDDSHMSNPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVG
CCCCCCEEEHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHEEHHC
GVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSGEVRPVPSGQERLKEAAKHGF
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCC
KRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE
CEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHCC
>Mature Secondary Structure 
AKAKRLYGCTECGATFPKWAGQCSECGAWNTLVETMIESGAAAPPTGRTGWTGSQAQIR
CCCHHHCCCHHHCCCCCHHCCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
TLAEVSVEEIPRFSTNSTELDRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRMP
HHHHHHHHHCCCCCCCCHHHHHHHCCCEECCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
ALYVTGEESQQQVAMRARRLGLPQDKLRVMTETCIETIIATAKVEKPKVMVIDSIQTIFT
EEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHH
EQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHEEEC
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATW
CCCCCHHHHHHHHHHHHCCHHHCCEECCCCHHHHHCCCCCEEEEEECHHHCCCCEEEEEE
EGTRPMLVEVQALVDDSHMSNPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVG
CCCCCCEEEHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHEEHHC
GVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSGEVRPVPSGQERLKEAAKHGF
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCC
KRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE
CEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]