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The map label for this gene is greB [H]

Identifier: 66047490

GI number: 66047490

Start: 5080858

End: 5081355

Strand: Direct

Name: greB [H]

Synonym: Psyr_4263

Alternate gene names: 66047490

Gene position: 5080858-5081355 (Clockwise)

Preceding gene: 66047489

Following gene: 66047492

Centisome position: 83.38

GC content: 62.05

Gene sequence:

>498_bases
ATGAGCCGCGCATTCGTAAACGAAGACAACGCTGCCGCCGAGGCCGAACAACCGGTAGAGCGGCTGGTCAGCACACAGAT
CAACTACGTCACTGCCGAAGGCCTGACCCTGTTGAAGAAACACGTCGGCAGCCTGCAGGCTCAGCACAGTATGCAAACTG
CGTTGGGCGAAGCGGCGGACAAGCAACGACTGGCCGACCTGGAACGCGACCTGCGTTACTTCAACCAGCGCCTGCAAAGC
GCGCAGGTCGTCGCCCCGGCTGTGTCGACCGAAAAAGTGCAGATCGGCAGTTGGGTGACGTTCGCGGATGAACAGGACAA
CCGCCAGCGCGTGCATCTGGTCGGAGAGGATCAGGCCGATGCAGCCAACGGGCTGATCAATTGGGGGTCGCCACTGGGCC
GCGCCCTGATCGGCGCGCAGAAAGGCGATGAAGTGTTGTGGCAGCGCCCGGCGGGCGATCAGTCGATTGAAGTGCTGCTG
ATCGAACCTGACGCGTGA

Upstream 100 bases:

>100_bases
CCGCCAAGGCCTATCGTCCGAACTGACCCGCAAAGCGTGGAAAATCGACGAAACCAATGATGTCCTGCTGTGGCCCAAAG
CCTATTAGGGAGGACTTTTG

Downstream 100 bases:

>100_bases
GCCAGGCCAGCCAACGGCAGCGCCGCTCCTTGCAAGGAGCGGCGCGTGCCTGAACGCTGATCAGGCCAGCTTTTTGTGAC
GCACACGATGCGGCTGGGCA

Product: transcription elongation factor GreA/GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MSRAFVNEDNAAAEAEQPVERLVSTQINYVTAEGLTLLKKHVGSLQAQHSMQTALGEAADKQRLADLERDLRYFNQRLQS
AQVVAPAVSTEKVQIGSWVTFADEQDNRQRVHLVGEDQADAANGLINWGSPLGRALIGAQKGDEVLWQRPAGDQSIEVLL
IEPDA

Sequences:

>Translated_165_residues
MSRAFVNEDNAAAEAEQPVERLVSTQINYVTAEGLTLLKKHVGSLQAQHSMQTALGEAADKQRLADLERDLRYFNQRLQS
AQVVAPAVSTEKVQIGSWVTFADEQDNRQRVHLVGEDQADAANGLINWGSPLGRALIGAQKGDEVLWQRPAGDQSIEVLL
IEPDA
>Mature_164_residues
SRAFVNEDNAAAEAEQPVERLVSTQINYVTAEGLTLLKKHVGSLQAQHSMQTALGEAADKQRLADLERDLRYFNQRLQSA
QVVAPAVSTEKVQIGSWVTFADEQDNRQRVHLVGEDQADAANGLINWGSPLGRALIGAQKGDEVLWQRPAGDQSIEVLLI
EPDA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18072; Mature: 17941

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAFVNEDNAAAEAEQPVERLVSTQINYVTAEGLTLLKKHVGSLQAQHSMQTALGEAAD
CCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQRLADLERDLRYFNQRLQSAQVVAPAVSTEKVQIGSWVTFADEQDNRQRVHLVGEDQAD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEECCCCCCCEEEEEECCCCCC
AANGLINWGSPLGRALIGAQKGDEVLWQRPAGDQSIEVLLIEPDA
HHCCCCCCCCHHHHHHHCCCCCCHHEEECCCCCCCEEEEEECCCC
>Mature Secondary Structure 
SRAFVNEDNAAAEAEQPVERLVSTQINYVTAEGLTLLKKHVGSLQAQHSMQTALGEAAD
CCCCCCCCCCHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQRLADLERDLRYFNQRLQSAQVVAPAVSTEKVQIGSWVTFADEQDNRQRVHLVGEDQAD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEECCCCCCCEEEEEECCCCCC
AANGLINWGSPLGRALIGAQKGDEVLWQRPAGDQSIEVLLIEPDA
HHCCCCCCCCHHHHHHHCCCCCCHHEEECCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]