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The map label for this gene is prfC [H]

Identifier: 66047480

GI number: 66047480

Start: 5070353

End: 5071936

Strand: Direct

Name: prfC [H]

Synonym: Psyr_4253

Alternate gene names: 66047480

Gene position: 5070353-5071936 (Clockwise)

Preceding gene: 66047475

Following gene: 66047481

Centisome position: 83.21

GC content: 60.16

Gene sequence:

>1584_bases
ATGACCCAACAGGCCGCCGAAGTCGCGAAACGCCGCACTTTCGCCATTATTTCCCACCCGGATGCGGGTAAAACCACCAT
CACGGAAAAACTCCTGCTGATGGGCAAGGCCATTTCCGTCGCCGGTACGGTCAAGTCGCGTAAATCCGACCGTCACGCCA
CCTCCGACTGGATGGAAATGGAGAAACAGCGTGGTATCTCCATTACCACGTCCGTGATGCAGTTTCCGTATCGCGACCAC
ATGATCAACCTGCTCGACACCCCGGGCCACGAAGACTTCTCCGAAGACACCTACCGCACCCTGACGGCGGTCGACTCGGC
CTTGATGGTCCTCGACGGCGGTAAAGGCGTCGAGCCACGCACCATCGCGCTGATGGATGTCTGCCGTCTGCGCGACACGC
CTATCGTCAGCTTCATCAACAAGCTCGACCGCGATATCCGCGACCCGATCGAGCTGCTCGATGAAATCGAAGCGGTCCTC
AAGATCAAGGCGGCGCCGATCACCTGGCCGATCGGTTGCTACCGGGACTTCAAGGGTGTCTATCACCTGGCCGACGACTA
CATCATTGTCTACACCGCAGGCCACGGCCACGAGCGTACCGAAACCAAAATCATCGAGAAGCTCGATTCTGACGAAGCCC
GCGCCCACCTGGGCGATGAATACGAGCGCTTCGTCGAGCAACTGGAGCTGGTACAGGGCGCCTGCCACGAATTCAACCAG
CAGGAATTTATCGACGGTCAGCTGACCCCGGTGTTCTTCGGTACCGCGCTGGGCAACTTTGGTGTCGATCATGTACTCGA
TGCCGTGGTGAACTGGGCGCCCAAGCCGTTGGCACGTGTGGCCAACGAACGCACCGTCGAGCCGGCGGAGGAGAAATTCA
GCGGTTTCGTGTTCAAGATCCAGGCGAACATGGACCCCAAGCACCGCGACCGCATCGCCTTCATGCGTATCTGCTCGGGC
CGTTACGACAAGGGCATGAAAATGCGCCATGTGCGCCTGGGCAAGGACGTACGGATCGGCGACGCGCTGACGTTCTTCTC
GTCGGAGCGCGAGCAGCTGGAAGAAGCCTACGCCGGCGACATCATCGGCTTGCACAACCACGGCACCATCCAGATCGGCG
ATACCTTTACCGAAGGCGAAGCGCTGGGCTTCACCGGTATTCCGCACTTCGCCCCGGAGCTGTTTCGTCGCGTCCGCCTG
AAGGACCCGCTCAAATCCAAGCAACTGCGTCAGGGTCTGCAACAGCTGGCCGAAGAAGGCGCCACACAGGTGTTCTTCCC
GCAGCGTAGCAACGACATCATCCTCGGCGCCGTCGGTGTGCTGCAGTTCGATGTGGTCGCCAGCCGCCTCAAGGAAGAAT
ACAAGGTCGAATGCGCCTACGAGCCTATCACTGTGTGGTCAGCGCGCTGGATCGACTGCGACGACAAGAAGAAGCTCGAA
GAGTTTGAGAACAAGGCGGTGGAAAACCTGGCAGTGGACGGCGGTGGTCACCTGACCTACCTCGCCCCTACCCGCGTGAA
CCTGGCGCTGATGGAAGAACGCTGGCCCGACGTGAAATTCCGCGCCACCCGCGAGCACCACTGA

Upstream 100 bases:

>100_bases
ACATTATCTGGCCATCGTTTATTGTCTGAACGGTTCGGTTATTGCCGCGAGTTGAGCTATAATCTGCGCCCTTTTTCAAT
CCCATCCCAGGCGATTTCCC

Downstream 100 bases:

>100_bases
CCGGCCGATGGCGTCGCTGGGTGACGCGGAGCGTCACGAACGGCATTCCCACGCGGGAGCGTGAGGAACGATAGGTCTGT
AACTATCGTGCCGATGCTCC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MTQQAAEVAKRRTFAIISHPDAGKTTITEKLLLMGKAISVAGTVKSRKSDRHATSDWMEMEKQRGISITTSVMQFPYRDH
MINLLDTPGHEDFSEDTYRTLTAVDSALMVLDGGKGVEPRTIALMDVCRLRDTPIVSFINKLDRDIRDPIELLDEIEAVL
KIKAAPITWPIGCYRDFKGVYHLADDYIIVYTAGHGHERTETKIIEKLDSDEARAHLGDEYERFVEQLELVQGACHEFNQ
QEFIDGQLTPVFFGTALGNFGVDHVLDAVVNWAPKPLARVANERTVEPAEEKFSGFVFKIQANMDPKHRDRIAFMRICSG
RYDKGMKMRHVRLGKDVRIGDALTFFSSEREQLEEAYAGDIIGLHNHGTIQIGDTFTEGEALGFTGIPHFAPELFRRVRL
KDPLKSKQLRQGLQQLAEEGATQVFFPQRSNDIILGAVGVLQFDVVASRLKEEYKVECAYEPITVWSARWIDCDDKKKLE
EFENKAVENLAVDGGGHLTYLAPTRVNLALMEERWPDVKFRATREHH

Sequences:

>Translated_527_residues
MTQQAAEVAKRRTFAIISHPDAGKTTITEKLLLMGKAISVAGTVKSRKSDRHATSDWMEMEKQRGISITTSVMQFPYRDH
MINLLDTPGHEDFSEDTYRTLTAVDSALMVLDGGKGVEPRTIALMDVCRLRDTPIVSFINKLDRDIRDPIELLDEIEAVL
KIKAAPITWPIGCYRDFKGVYHLADDYIIVYTAGHGHERTETKIIEKLDSDEARAHLGDEYERFVEQLELVQGACHEFNQ
QEFIDGQLTPVFFGTALGNFGVDHVLDAVVNWAPKPLARVANERTVEPAEEKFSGFVFKIQANMDPKHRDRIAFMRICSG
RYDKGMKMRHVRLGKDVRIGDALTFFSSEREQLEEAYAGDIIGLHNHGTIQIGDTFTEGEALGFTGIPHFAPELFRRVRL
KDPLKSKQLRQGLQQLAEEGATQVFFPQRSNDIILGAVGVLQFDVVASRLKEEYKVECAYEPITVWSARWIDCDDKKKLE
EFENKAVENLAVDGGGHLTYLAPTRVNLALMEERWPDVKFRATREHH
>Mature_526_residues
TQQAAEVAKRRTFAIISHPDAGKTTITEKLLLMGKAISVAGTVKSRKSDRHATSDWMEMEKQRGISITTSVMQFPYRDHM
INLLDTPGHEDFSEDTYRTLTAVDSALMVLDGGKGVEPRTIALMDVCRLRDTPIVSFINKLDRDIRDPIELLDEIEAVLK
IKAAPITWPIGCYRDFKGVYHLADDYIIVYTAGHGHERTETKIIEKLDSDEARAHLGDEYERFVEQLELVQGACHEFNQQ
EFIDGQLTPVFFGTALGNFGVDHVLDAVVNWAPKPLARVANERTVEPAEEKFSGFVFKIQANMDPKHRDRIAFMRICSGR
YDKGMKMRHVRLGKDVRIGDALTFFSSEREQLEEAYAGDIIGLHNHGTIQIGDTFTEGEALGFTGIPHFAPELFRRVRLK
DPLKSKQLRQGLQQLAEEGATQVFFPQRSNDIILGAVGVLQFDVVASRLKEEYKVECAYEPITVWSARWIDCDDKKKLEE
FENKAVENLAVDGGGHLTYLAPTRVNLALMEERWPDVKFRATREHH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=484, Percent_Identity=28.099173553719, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI19923640, Length=514, Percent_Identity=26.8482490272374, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI25306283, Length=399, Percent_Identity=28.8220551378446, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI25306287, Length=486, Percent_Identity=25.5144032921811, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI157426893, Length=157, Percent_Identity=30.5732484076433, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI94966754, Length=150, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI310132016, Length=124, Percent_Identity=32.258064516129, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310110807, Length=124, Percent_Identity=32.258064516129, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310123363, Length=124, Percent_Identity=32.258064516129, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=66.9865642994242, Blast_Score=739, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=483, Percent_Identity=28.7784679089027, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1788922, Length=158, Percent_Identity=34.1772151898734, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=34.8993288590604, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=488, Percent_Identity=28.6885245901639, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17556745, Length=178, Percent_Identity=33.7078651685393, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557151, Length=134, Percent_Identity=32.089552238806, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=30.8270676691729, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=30.8270676691729, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=476, Percent_Identity=29.6218487394958, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6322359, Length=425, Percent_Identity=26.8235294117647, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=27.8145695364238, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=156, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=156, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24582462, Length=476, Percent_Identity=29.8319327731092, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI221458488, Length=511, Percent_Identity=24.6575342465753, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=30.188679245283, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59721; Mature: 59590

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQAAEVAKRRTFAIISHPDAGKTTITEKLLLMGKAISVAGTVKSRKSDRHATSDWMEM
CCCHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
EKQRGISITTSVMQFPYRDHMINLLDTPGHEDFSEDTYRTLTAVDSALMVLDGGKGVEPR
HHHCCCEEEHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCH
TIALMDVCRLRDTPIVSFINKLDRDIRDPIELLDEIEAVLKIKAAPITWPIGCYRDFKGV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHH
YHLADDYIIVYTAGHGHERTETKIIEKLDSDEARAHLGDEYERFVEQLELVQGACHEFNQ
HEECCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
QEFIDGQLTPVFFGTALGNFGVDHVLDAVVNWAPKPLARVANERTVEPAEEKFSGFVFKI
HHHCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHCCEEEEE
QANMDPKHRDRIAFMRICSGRYDKGMKMRHVRLGKDVRIGDALTFFSSEREQLEEAYAGD
ECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEECHHHHHHHHHHHHHHHHHCCC
IIGLHNHGTIQIGDTFTEGEALGFTGIPHFAPELFRRVRLKDPLKSKQLRQGLQQLAEEG
EEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
ATQVFFPQRSNDIILGAVGVLQFDVVASRLKEEYKVECAYEPITVWSARWIDCDDKKKLE
CCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEEEEECCCCHHHHH
EFENKAVENLAVDGGGHLTYLAPTRVNLALMEERWPDVKFRATREHH
HHHHHHHHHEEECCCCEEEEECCCCEEEEEHHHCCCCCEEECCCCCC
>Mature Secondary Structure 
TQQAAEVAKRRTFAIISHPDAGKTTITEKLLLMGKAISVAGTVKSRKSDRHATSDWMEM
CCHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
EKQRGISITTSVMQFPYRDHMINLLDTPGHEDFSEDTYRTLTAVDSALMVLDGGKGVEPR
HHHCCCEEEHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCH
TIALMDVCRLRDTPIVSFINKLDRDIRDPIELLDEIEAVLKIKAAPITWPIGCYRDFKGV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHH
YHLADDYIIVYTAGHGHERTETKIIEKLDSDEARAHLGDEYERFVEQLELVQGACHEFNQ
HEECCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
QEFIDGQLTPVFFGTALGNFGVDHVLDAVVNWAPKPLARVANERTVEPAEEKFSGFVFKI
HHHCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHCCEEEEE
QANMDPKHRDRIAFMRICSGRYDKGMKMRHVRLGKDVRIGDALTFFSSEREQLEEAYAGD
ECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEECHHHHHHHHHHHHHHHHHCCC
IIGLHNHGTIQIGDTFTEGEALGFTGIPHFAPELFRRVRLKDPLKSKQLRQGLQQLAEEG
EEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
ATQVFFPQRSNDIILGAVGVLQFDVVASRLKEEYKVECAYEPITVWSARWIDCDDKKKLE
CCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEEEEECCCCHHHHH
EFENKAVENLAVDGGGHLTYLAPTRVNLALMEERWPDVKFRATREHH
HHHHHHHHHEEECCCCEEEEECCCCEEEEEHHHCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA