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The map label for this gene is gsiB [H]

Identifier: 66047438

GI number: 66047438

Start: 5015990

End: 5017504

Strand: Reverse

Name: gsiB [H]

Synonym: Psyr_4211

Alternate gene names: 66047438

Gene position: 5017504-5015990 (Counterclockwise)

Preceding gene: 66047439

Following gene: 66047437

Centisome position: 82.34

GC content: 59.14

Gene sequence:

>1515_bases
ATGAAAGCTCGACAAATGAAAATGCTGACCGTCGCCATGCTGGCGGCCTGTTCCCTGGCGGCGGGCGTGGCCGAAGCTGC
GGGCGTGCTGACCATCGGCCGTCGTGAGGATGGCACCACGTTCGACCCGATCAAAAGCGCGCAGAACATCGACAACTGGG
TGTTTTCCAACGTCTACGACGTGCTGATCCGCGTAGATGACAAAGGCACCAAGCTGGTACCGGGGCTGGCGGAAAGCTGG
TCGATCTCGCCTGACGGGCTGACCTACACGCTCAAGATGCGCCCGGCCAAATTCTCCGATGGCTCGGACCTGACGGCCAG
TGACGCGGTGTTCAGCCTGCTGCGAATCCGCGACGACAAGGGTTCACTGTGGAGCGATTCCTACAAGATTATCGACAAGG
CCGTCGCGACAGATCCGCATACGCTGGTGATCACGCTGAAGAATCCTTCGGCCTCGTTCCTCTCGCAACTGGCCCTGCCC
AACGTGTCGATTCTCTCCGAGAAAGCCATGAAGAGCATGGGCGAAGAGGCGTATGCCGAGCAGCCTGTCAGCTCCGGCGC
ATTTACCGTGAAGGAATGGCGGCGCGGTGACCGGGTGATTCTGGCGAAGAACCCCAACTTCTGGGAAGCACCGAGCGTTA
GCCTGGACGGGGTCGAGTGGATCTCGGTGCCGGACGATAACACCCGCACGCTCATGGTCCAGAAGGGCGAGCTCGATGCT
GCGATATGGATACCGTTCTCGCGCATCGCAAATCTGCAGAAGGACCCCGATCTGGTGGTCCATCTCGACCCCTCCACCCG
TGAAGATCATCTGCTGATCAACCACGAACATGGCGCATTGGCCAAGCCGGAAGTGCGCCAGGCGCTGGACATGGCCATCG
ACAAGAAATCGCTGGTCGATGCCGCCACTTTCGGGCTGGGCACCGTGGCCAATTCCTACATTCCCAAAGGCGCGCTGTAC
CACTACGCCGACAACCTGCAACGTCCTTATGACCCGGTGAAGGCCAGACAGATGCTGGCGGACGCCGGTGCATCGGACCT
GAAACTCAATTACGTGGTCAACGCGGGCAACGAGGTCGACGAGCAAATCGCGGTCATGCTGCAACAGCAACTGGCCAAGG
TCGGCGTGACCACAACGCTGCAGAAAGTCGACCCGAGCCAGAGCTGGGACATGTTGATTGCCGGTGACTACGACATCTCG
GTGATGTACTGGACCAACGACATCCTCGACCCGGACCAGAAGACCACCTTCGTGCTCGGCCACGACACCAACAATAACTA
CATGACCCGTTACAAGAACGACAAGGTCAAGGATCTGGTCGCTGCCGCACGGGTCGAGATGGACCCGAAAAAGCGTGAGC
AGATGTACATCGACCTGCAGAAAATGGCCAAGGCGGACGTCAACTGGATCGACCTCTACTACAGTCCCTACATCAGCGTG
TCGCGCAAGAACATCGAGCACTTCGGCCAGAACCCGCTGGGCCGCTTTACGCTCGAAGAAACGGTCAAGAAGTGA

Upstream 100 bases:

>100_bases
ATGTCGGCCAGCAGCGCTACGACTGCGTGCTGGAAGCCCCGGTTTTTTGATCTCAACCTGTAACGACATCTGTCCCGACA
TCGACAAGGAGTTCATGCGT

Downstream 100 bases:

>100_bases
CATGAGCAGGGCGCCTGTCTGAACGGCAGGCGCTATTCAGCAACGCTTTCCGATGCCTATTCGGCCGGGAAACGTTAATT
TGTATCCTCAGGTTTACCAG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MKARQMKMLTVAMLAACSLAAGVAEAAGVLTIGRREDGTTFDPIKSAQNIDNWVFSNVYDVLIRVDDKGTKLVPGLAESW
SISPDGLTYTLKMRPAKFSDGSDLTASDAVFSLLRIRDDKGSLWSDSYKIIDKAVATDPHTLVITLKNPSASFLSQLALP
NVSILSEKAMKSMGEEAYAEQPVSSGAFTVKEWRRGDRVILAKNPNFWEAPSVSLDGVEWISVPDDNTRTLMVQKGELDA
AIWIPFSRIANLQKDPDLVVHLDPSTREDHLLINHEHGALAKPEVRQALDMAIDKKSLVDAATFGLGTVANSYIPKGALY
HYADNLQRPYDPVKARQMLADAGASDLKLNYVVNAGNEVDEQIAVMLQQQLAKVGVTTTLQKVDPSQSWDMLIAGDYDIS
VMYWTNDILDPDQKTTFVLGHDTNNNYMTRYKNDKVKDLVAAARVEMDPKKREQMYIDLQKMAKADVNWIDLYYSPYISV
SRKNIEHFGQNPLGRFTLEETVKK

Sequences:

>Translated_504_residues
MKARQMKMLTVAMLAACSLAAGVAEAAGVLTIGRREDGTTFDPIKSAQNIDNWVFSNVYDVLIRVDDKGTKLVPGLAESW
SISPDGLTYTLKMRPAKFSDGSDLTASDAVFSLLRIRDDKGSLWSDSYKIIDKAVATDPHTLVITLKNPSASFLSQLALP
NVSILSEKAMKSMGEEAYAEQPVSSGAFTVKEWRRGDRVILAKNPNFWEAPSVSLDGVEWISVPDDNTRTLMVQKGELDA
AIWIPFSRIANLQKDPDLVVHLDPSTREDHLLINHEHGALAKPEVRQALDMAIDKKSLVDAATFGLGTVANSYIPKGALY
HYADNLQRPYDPVKARQMLADAGASDLKLNYVVNAGNEVDEQIAVMLQQQLAKVGVTTTLQKVDPSQSWDMLIAGDYDIS
VMYWTNDILDPDQKTTFVLGHDTNNNYMTRYKNDKVKDLVAAARVEMDPKKREQMYIDLQKMAKADVNWIDLYYSPYISV
SRKNIEHFGQNPLGRFTLEETVKK
>Mature_504_residues
MKARQMKMLTVAMLAACSLAAGVAEAAGVLTIGRREDGTTFDPIKSAQNIDNWVFSNVYDVLIRVDDKGTKLVPGLAESW
SISPDGLTYTLKMRPAKFSDGSDLTASDAVFSLLRIRDDKGSLWSDSYKIIDKAVATDPHTLVITLKNPSASFLSQLALP
NVSILSEKAMKSMGEEAYAEQPVSSGAFTVKEWRRGDRVILAKNPNFWEAPSVSLDGVEWISVPDDNTRTLMVQKGELDA
AIWIPFSRIANLQKDPDLVVHLDPSTREDHLLINHEHGALAKPEVRQALDMAIDKKSLVDAATFGLGTVANSYIPKGALY
HYADNLQRPYDPVKARQMLADAGASDLKLNYVVNAGNEVDEQIAVMLQQQLAKVGVTTTLQKVDPSQSWDMLIAGDYDIS
VMYWTNDILDPDQKTTFVLGHDTNNNYMTRYKNDKVKDLVAAARVEMDPKKREQMYIDLQKMAKADVNWIDLYYSPYISV
SRKNIEHFGQNPLGRFTLEETVKK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=504, Percent_Identity=30.3571428571429, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1787762, Length=500, Percent_Identity=27.4, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1789966, Length=473, Percent_Identity=26.4270613107822, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1787551, Length=492, Percent_Identity=26.0162601626016, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789397, Length=515, Percent_Identity=25.0485436893204, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1787495, Length=514, Percent_Identity=24.7081712062257, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789887, Length=381, Percent_Identity=25.4593175853018, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI87081878, Length=469, Percent_Identity=23.8805970149254, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56069; Mature: 56069

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKARQMKMLTVAMLAACSLAAGVAEAAGVLTIGRREDGTTFDPIKSAQNIDNWVFSNVYD
CCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEE
VLIRVDDKGTKLVPGLAESWSISPDGLTYTLKMRPAKFSDGSDLTASDAVFSLLRIRDDK
EEEEECCCCCEECCCCHHCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHEECCC
GSLWSDSYKIIDKAVATDPHTLVITLKNPSASFLSQLALPNVSILSEKAMKSMGEEAYAE
CCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHCHHHHHC
QPVSSGAFTVKEWRRGDRVILAKNPNFWEAPSVSLDGVEWISVPDDNTRTLMVQKGELDA
CCCCCCCEEHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCE
AIWIPFSRIANLQKDPDLVVHLDPSTREDHLLINHEHGALAKPEVRQALDMAIDKKSLVD
EEEEEHHHHCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCHHHHHH
AATFGLGTVANSYIPKGALYHYADNLQRPYDPVKARQMLADAGASDLKLNYVVNAGNEVD
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCHH
EQIAVMLQQQLAKVGVTTTLQKVDPSQSWDMLIAGDYDISVMYWTNDILDPDQKTTFVLG
HHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEECCCEEEEEEEECCCCCCCCCEEEEEE
HDTNNNYMTRYKNDKVKDLVAAARVEMDPKKREQMYIDLQKMAKADVNWIDLYYSPYISV
ECCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEE
SRKNIEHFGQNPLGRFTLEETVKK
EHHHHHHHCCCCCCCEEHHHHHCC
>Mature Secondary Structure
MKARQMKMLTVAMLAACSLAAGVAEAAGVLTIGRREDGTTFDPIKSAQNIDNWVFSNVYD
CCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEE
VLIRVDDKGTKLVPGLAESWSISPDGLTYTLKMRPAKFSDGSDLTASDAVFSLLRIRDDK
EEEEECCCCCEECCCCHHCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHEECCC
GSLWSDSYKIIDKAVATDPHTLVITLKNPSASFLSQLALPNVSILSEKAMKSMGEEAYAE
CCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHCHHHHHC
QPVSSGAFTVKEWRRGDRVILAKNPNFWEAPSVSLDGVEWISVPDDNTRTLMVQKGELDA
CCCCCCCEEHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCE
AIWIPFSRIANLQKDPDLVVHLDPSTREDHLLINHEHGALAKPEVRQALDMAIDKKSLVD
EEEEEHHHHCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCHHHHHH
AATFGLGTVANSYIPKGALYHYADNLQRPYDPVKARQMLADAGASDLKLNYVVNAGNEVD
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCHH
EQIAVMLQQQLAKVGVTTTLQKVDPSQSWDMLIAGDYDISVMYWTNDILDPDQKTTFVLG
HHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEECCCEEEEEEEECCCCCCCCCEEEEEE
HDTNNNYMTRYKNDKVKDLVAAARVEMDPKKREQMYIDLQKMAKADVNWIDLYYSPYISV
ECCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEE
SRKNIEHFGQNPLGRFTLEETVKK
EHHHHHHHCCCCCCCEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA