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The map label for this gene is grpE [H]

Identifier: 66047423

GI number: 66047423

Start: 4992730

End: 4993293

Strand: Reverse

Name: grpE [H]

Synonym: Psyr_4196

Alternate gene names: 66047423

Gene position: 4993293-4992730 (Counterclockwise)

Preceding gene: 66047425

Following gene: 66047422

Centisome position: 81.94

GC content: 58.87

Gene sequence:

>564_bases
ATGGCTGACGAACAGAATCTGGATGCGCAGGCTCAGGATCAGGCTGCCGAGGCGGGCGCGGGTGACGAACTGACAACCCG
CGTGCAGGTGCTCGAAGAGCAACTGGCTGCTGCGCAGGACCAATCCCTTCGCGTGGCTGCGGATCTGCAGAATGTCCGCC
GCCGTGCCGAACAGGATGTTGAAAAGGCGCACAAGTTTGCGCTGGAGAAATTTGCCGGTGATCTGTTGCCGATCATCGAC
AGTCTGGAGCGAGGCCTTGATCTGTCCAGTCCGGACGATGAAAGCATTCGCCCGATGCGTGAAGGCATCGAGCTGACCTT
GAAGATGTTCCAGGACACCCTCAAGCGTTATCAGCTGGAAGCCATCGATCCGCACGGTCAACCGTTCAGTGCCGACCAGC
ACCAGGCGATGGCCATGCAGGAAAGTGCCGACGTTGAGCCCAACACGGTGCTCAAGGTATTTCAGAAGGGTTATCAGCTT
AATGGTCGCCTGTTGCGCCCGGCCATGGTCGTGGTCAGCAAGGCACCATCGCCTGCAACGCCGTCAATCAACGAGCAGGC
TTGA

Upstream 100 bases:

>100_bases
ATCTCGATCGCACGAAACCCTTGAAGCCGGAAAATCCGATCCCATATAGGTGGGCAGAAGCGCGAATCGGGATCGCGGAC
GTTTTTAGAGGAGAACATGT

Downstream 100 bases:

>100_bases
AATCAGCTGTAAGGCCCCCATTTAAAGGTCAAGCGTTTAAGAGCTACCGCACGGTGTAAACGCACTGGCGGCAACCAAAT
CCAAGTTTTGCAAGTTTCGG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MADEQNLDAQAQDQAAEAGAGDELTTRVQVLEEQLAAAQDQSLRVAADLQNVRRRAEQDVEKAHKFALEKFAGDLLPIID
SLERGLDLSSPDDESIRPMREGIELTLKMFQDTLKRYQLEAIDPHGQPFSADQHQAMAMQESADVEPNTVLKVFQKGYQL
NGRLLRPAMVVVSKAPSPATPSINEQA

Sequences:

>Translated_187_residues
MADEQNLDAQAQDQAAEAGAGDELTTRVQVLEEQLAAAQDQSLRVAADLQNVRRRAEQDVEKAHKFALEKFAGDLLPIID
SLERGLDLSSPDDESIRPMREGIELTLKMFQDTLKRYQLEAIDPHGQPFSADQHQAMAMQESADVEPNTVLKVFQKGYQL
NGRLLRPAMVVVSKAPSPATPSINEQA
>Mature_186_residues
ADEQNLDAQAQDQAAEAGAGDELTTRVQVLEEQLAAAQDQSLRVAADLQNVRRRAEQDVEKAHKFALEKFAGDLLPIIDS
LERGLDLSSPDDESIRPMREGIELTLKMFQDTLKRYQLEAIDPHGQPFSADQHQAMAMQESADVEPNTVLKVFQKGYQLN
GRLLRPAMVVVSKAPSPATPSINEQA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=149, Percent_Identity=32.8859060402685, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI40255109, Length=137, Percent_Identity=30.6569343065693, Blast_Score=70, Evalue=8e-13,
Organism=Escherichia coli, GI1788967, Length=153, Percent_Identity=51.6339869281046, Blast_Score=151, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17552458, Length=164, Percent_Identity=31.0975609756098, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=161, Percent_Identity=34.7826086956522, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653432, Length=158, Percent_Identity=34.8101265822785, Blast_Score=97, Evalue=6e-21,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 20649; Mature: 20518

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEQNLDAQAQDQAAEAGAGDELTTRVQVLEEQLAAAQDQSLRVAADLQNVRRRAEQDV
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EKAHKFALEKFAGDLLPIIDSLERGLDLSSPDDESIRPMREGIELTLKMFQDTLKRYQLE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIDPHGQPFSADQHQAMAMQESADVEPNTVLKVFQKGYQLNGRLLRPAMVVVSKAPSPAT
HCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCC
PSINEQA
CCCCCCC
>Mature Secondary Structure 
ADEQNLDAQAQDQAAEAGAGDELTTRVQVLEEQLAAAQDQSLRVAADLQNVRRRAEQDV
CCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EKAHKFALEKFAGDLLPIIDSLERGLDLSSPDDESIRPMREGIELTLKMFQDTLKRYQLE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIDPHGQPFSADQHQAMAMQESADVEPNTVLKVFQKGYQLNGRLLRPAMVVVSKAPSPAT
HCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCC
PSINEQA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA