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The map label for this gene is rlmE [H]

Identifier: 66047415

GI number: 66047415

Start: 4982078

End: 4982728

Strand: Reverse

Name: rlmE [H]

Synonym: Psyr_4188

Alternate gene names: 66047415

Gene position: 4982728-4982078 (Counterclockwise)

Preceding gene: 66047417

Following gene: 66047414

Centisome position: 81.77

GC content: 56.07

Gene sequence:

>651_bases
GTGGCCCGTTCCAAGACAAGCCATAACTGGCTCAAAGAACACTTCGACGACAAGTACGTCAAAATGGCGCAGAAGGACGG
CTACCGTTCGCGCGCCAGTTACAAGCTGCTCGAGATCCAGGAAAAGGACAAACTCATCCGTCCGGGCATGACCGTGATCG
ACCTCGGCGCAGCGCCGGGAGGCTGGTCACAGGTGACCAGTCGTCTGATCGGCGGTCAAGGTCGTCTGATCGCTTCCGAC
ATCCTTGAAATGGACAGCATCCCCGATGTGACCTTCATTCAGGGGGATTTTACCGAGGATGCGATTCTTGAGCAGATCCT
CGAGGCAGTCGGTAATACGCAGGTAGACCTTGTGATTTCCGATATGGCCCCCAATATGAGTGGATTGAGCGCTGTAGATA
TGCCGCGTGCAATGTTTCTCTGCGAATTGGCGCTCGATCTTGCCGGTCGGGTACTGCGTCCCGGTGGCGATTTTCTGATC
AAGGTCTTCCAGGGTGAAGGCTTCGATGTCTACCACAAGGACATTCGCAAGCTGTTCGACAAGGTGCAGATGCGCAAACC
GTCGTCGTCCCGCGACAGGTCACGCGAGCAATACCTGCTGGCTCGCGGCTTTCGCGGAATTGATGGCGCGGCCAGCATCG
AACGTTTTTGA

Upstream 100 bases:

>100_bases
CTGACCTCTGCGTCTGATCTTTTAAAAGCGGCGGCCAGTTTACCCGAGCGAGTCCGGGACCGCCCAGTTAATCACGACTC
GTTTATTTTTGAGGTGCCCC

Downstream 100 bases:

>100_bases
CAAGGCGCGATAGCTTTTTTCAAATGGCGTTACCGATGCAGCATGACTGAACATTATGTTGTCAAAGTTTCACAAAGGGT
TACAGACGGTGTCTGCCGCA

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MARSKTSHNWLKEHFDDKYVKMAQKDGYRSRASYKLLEIQEKDKLIRPGMTVIDLGAAPGGWSQVTSRLIGGQGRLIASD
ILEMDSIPDVTFIQGDFTEDAILEQILEAVGNTQVDLVISDMAPNMSGLSAVDMPRAMFLCELALDLAGRVLRPGGDFLI
KVFQGEGFDVYHKDIRKLFDKVQMRKPSSSRDRSREQYLLARGFRGIDGAASIERF

Sequences:

>Translated_216_residues
MARSKTSHNWLKEHFDDKYVKMAQKDGYRSRASYKLLEIQEKDKLIRPGMTVIDLGAAPGGWSQVTSRLIGGQGRLIASD
ILEMDSIPDVTFIQGDFTEDAILEQILEAVGNTQVDLVISDMAPNMSGLSAVDMPRAMFLCELALDLAGRVLRPGGDFLI
KVFQGEGFDVYHKDIRKLFDKVQMRKPSSSRDRSREQYLLARGFRGIDGAASIERF
>Mature_215_residues
ARSKTSHNWLKEHFDDKYVKMAQKDGYRSRASYKLLEIQEKDKLIRPGMTVIDLGAAPGGWSQVTSRLIGGQGRLIASDI
LEMDSIPDVTFIQGDFTEDAILEQILEAVGNTQVDLVISDMAPNMSGLSAVDMPRAMFLCELALDLAGRVLRPGGDFLIK
VFQGEGFDVYHKDIRKLFDKVQMRKPSSSRDRSREQYLLARGFRGIDGAASIERF

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=217, Percent_Identity=34.5622119815668, Blast_Score=147, Evalue=6e-36,
Organism=Homo sapiens, GI29029591, Length=195, Percent_Identity=36.4102564102564, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI29029589, Length=195, Percent_Identity=36.4102564102564, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI7110661, Length=195, Percent_Identity=36.4102564102564, Blast_Score=135, Evalue=2e-32,
Organism=Homo sapiens, GI194097365, Length=196, Percent_Identity=30.6122448979592, Blast_Score=114, Evalue=5e-26,
Organism=Escherichia coli, GI1789569, Length=201, Percent_Identity=61.6915422885572, Blast_Score=261, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17554650, Length=196, Percent_Identity=31.1224489795918, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17553474, Length=203, Percent_Identity=33.0049261083744, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71987550, Length=193, Percent_Identity=33.6787564766839, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71987561, Length=193, Percent_Identity=33.6787564766839, Blast_Score=107, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17553860, Length=193, Percent_Identity=33.6787564766839, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=35.678391959799, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319796, Length=192, Percent_Identity=34.375, Blast_Score=108, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6321302, Length=288, Percent_Identity=27.0833333333333, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356387, Length=213, Percent_Identity=35.2112676056338, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=32.3232323232323, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648639, Length=201, Percent_Identity=29.8507462686567, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859957, Length=192, Percent_Identity=30.2083333333333, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 24205; Mature: 24074

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSKTSHNWLKEHFDDKYVKMAQKDGYRSRASYKLLEIQEKDKLIRPGMTVIDLGAAPG
CCCCCCHHHHHHHHHCHHHHHHHHHCCCCCHHCCHHEEEHHHHHHHCCCCEEEECCCCCC
GWSQVTSRLIGGQGRLIASDILEMDSIPDVTFIQGDFTEDAILEQILEAVGNTQVDLVIS
CHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEE
DMAPNMSGLSAVDMPRAMFLCELALDLAGRVLRPGGDFLIKVFQGEGFDVYHKDIRKLFD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
KVQMRKPSSSRDRSREQYLLARGFRGIDGAASIERF
HHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure 
ARSKTSHNWLKEHFDDKYVKMAQKDGYRSRASYKLLEIQEKDKLIRPGMTVIDLGAAPG
CCCCCHHHHHHHHHCHHHHHHHHHCCCCCHHCCHHEEEHHHHHHHCCCCEEEECCCCCC
GWSQVTSRLIGGQGRLIASDILEMDSIPDVTFIQGDFTEDAILEQILEAVGNTQVDLVIS
CHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEE
DMAPNMSGLSAVDMPRAMFLCELALDLAGRVLRPGGDFLIKVFQGEGFDVYHKDIRKLFD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
KVQMRKPSSSRDRSREQYLLARGFRGIDGAASIERF
HHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA