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The map label for this gene is truB [H]

Identifier: 66047405

GI number: 66047405

Start: 4970069

End: 4970986

Strand: Reverse

Name: truB [H]

Synonym: Psyr_4178

Alternate gene names: 66047405

Gene position: 4970986-4970069 (Counterclockwise)

Preceding gene: 66047406

Following gene: 66047404

Centisome position: 81.58

GC content: 58.93

Gene sequence:

>918_bases
GTGGCTCAGGTCAAGCGTATACGCCGCAATGTCAGCGGGATCATCCTGCTCGACAAGCCACTGGGTTTCACCTCCAACGC
CGCTCTGCAAAAGGTTCGCTGGCTGCTCAACGCCGAGAAAGCCGGTCACACCGGCAGCCTCGATCCTCTGGCAACGGGCG
TTCTGCCGTTGTGCTTTGGTGAGGCGACCAAGTTCTCTCAGTACCTGCTCGATTCGGACAAGTCCTACGAGACCCTGGCG
CAACTGGGCAAGACCACTACCACTGCCGACTCTGAAGGCGAGGTTTTGCTCACGCGTCCGGTGACCGTTGGTCGCGACGA
TATCGAAGCGGCTCTGCCGCATTTTCGCGGACAAATCAGCCAGATACCTCCCATGTACTCGGCCCTCAAGCGTGATGGCC
AGCCACTTTACAAGCTGGCACGTGCAGGGGAAGTAGTGGAGCGCGAGCCCCGTTCTGTTACTATTGCGCGCCTGGAATTA
CTTGCTTGTGAAGGTGACACAGCACGTCTGTCCGTCGATTGCAGCAAAGGCACCTATATCCGCACGCTGGTCGAGGATAT
CGGTGAGAAGCTTGGTTGTGGTGCTTACGTCGCAGAGCTGCGTCGTACCCAGGCCGGGCCTTTCACGCTGGCTCAGACAG
TGACGCTGGAAGAGCTCGAGCAGGTGCATGCCGACGGCGGCAATGAAGCGGTTGACCGTTTCCTGATGCCATCGGACAGC
GGTCTGCTGGACTGGCCGCTGTTGAAGTTTTCGGAACACAGTTCGTTTTACTGGTTGCATGGTCAGCCAGTACGCGCGCC
GGACGCACCGAAATTCGGTATGGTTCGCGTGCAGGATCACGAAGGTCGCTTCATCGGTATCGGTGAAGTCGCCGAAGACG
GGCGCATTGCGCCGCGTCGCTTGATTCGGTCAGAGTGA

Upstream 100 bases:

>100_bases
TCTCTCGGCGCTGATCGAGCGTGCCGTGGCAGAAGACGGTCAGCATCAGGAAGGCCCTGCATCGGCGGATGCAAAGCCTG
AAAGTACCGAGGAATAAAGA

Downstream 100 bases:

>100_bases
CCGAAACCAGTCCAGCCGCCGGCTTGCCGGCGAGTGGGGTGGAACGAGGATGGCTGTCAACAGGCACGGTCATACCTCAT
TTTTTTAATACGAGGATCTG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MAQVKRIRRNVSGIILLDKPLGFTSNAALQKVRWLLNAEKAGHTGSLDPLATGVLPLCFGEATKFSQYLLDSDKSYETLA
QLGKTTTTADSEGEVLLTRPVTVGRDDIEAALPHFRGQISQIPPMYSALKRDGQPLYKLARAGEVVEREPRSVTIARLEL
LACEGDTARLSVDCSKGTYIRTLVEDIGEKLGCGAYVAELRRTQAGPFTLAQTVTLEELEQVHADGGNEAVDRFLMPSDS
GLLDWPLLKFSEHSSFYWLHGQPVRAPDAPKFGMVRVQDHEGRFIGIGEVAEDGRIAPRRLIRSE

Sequences:

>Translated_305_residues
MAQVKRIRRNVSGIILLDKPLGFTSNAALQKVRWLLNAEKAGHTGSLDPLATGVLPLCFGEATKFSQYLLDSDKSYETLA
QLGKTTTTADSEGEVLLTRPVTVGRDDIEAALPHFRGQISQIPPMYSALKRDGQPLYKLARAGEVVEREPRSVTIARLEL
LACEGDTARLSVDCSKGTYIRTLVEDIGEKLGCGAYVAELRRTQAGPFTLAQTVTLEELEQVHADGGNEAVDRFLMPSDS
GLLDWPLLKFSEHSSFYWLHGQPVRAPDAPKFGMVRVQDHEGRFIGIGEVAEDGRIAPRRLIRSE
>Mature_304_residues
AQVKRIRRNVSGIILLDKPLGFTSNAALQKVRWLLNAEKAGHTGSLDPLATGVLPLCFGEATKFSQYLLDSDKSYETLAQ
LGKTTTTADSEGEVLLTRPVTVGRDDIEAALPHFRGQISQIPPMYSALKRDGQPLYKLARAGEVVEREPRSVTIARLELL
ACEGDTARLSVDCSKGTYIRTLVEDIGEKLGCGAYVAELRRTQAGPFTLAQTVTLEELEQVHADGGNEAVDRFLMPSDSG
LLDWPLLKFSEHSSFYWLHGQPVRAPDAPKFGMVRVQDHEGRFIGIGEVAEDGRIAPRRLIRSE

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=221, Percent_Identity=41.1764705882353, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI215599015, Length=195, Percent_Identity=31.7948717948718, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI4503337, Length=195, Percent_Identity=31.7948717948718, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI2367200, Length=309, Percent_Identity=50.1618122977346, Blast_Score=309, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17553978, Length=195, Percent_Identity=29.2307692307692, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324037, Length=193, Percent_Identity=34.7150259067358, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=202, Percent_Identity=29.2079207920792, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281364189, Length=196, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364187, Length=196, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364185, Length=196, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364183, Length=196, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI62471759, Length=196, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17975520, Length=196, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33544; Mature: 33412

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQVKRIRRNVSGIILLDKPLGFTSNAALQKVRWLLNAEKAGHTGSLDPLATGVLPLCFG
CCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
EATKFSQYLLDSDKSYETLAQLGKTTTTADSEGEVLLTRPVTVGRDDIEAALPHFRGQIS
CHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
QIPPMYSALKRDGQPLYKLARAGEVVEREPRSVTIARLELLACEGDTARLSVDCSKGTYI
CCCHHHHHHHHCCHHHHHHHHHCHHHHCCCCEEEEEEEEEEEECCCCEEEEEECCCCCHH
RTLVEDIGEKLGCGAYVAELRRTQAGPFTLAQTVTLEELEQVHADGGNEAVDRFLMPSDS
HHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
GLLDWPLLKFSEHSSFYWLHGQPVRAPDAPKFGMVRVQDHEGRFIGIGEVAEDGRIAPRR
CCCCCCHHEECCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCHHH
LIRSE
HHCCC
>Mature Secondary Structure 
AQVKRIRRNVSGIILLDKPLGFTSNAALQKVRWLLNAEKAGHTGSLDPLATGVLPLCFG
CHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
EATKFSQYLLDSDKSYETLAQLGKTTTTADSEGEVLLTRPVTVGRDDIEAALPHFRGQIS
CHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
QIPPMYSALKRDGQPLYKLARAGEVVEREPRSVTIARLELLACEGDTARLSVDCSKGTYI
CCCHHHHHHHHCCHHHHHHHHHCHHHHCCCCEEEEEEEEEEEECCCCEEEEEECCCCCHH
RTLVEDIGEKLGCGAYVAELRRTQAGPFTLAQTVTLEELEQVHADGGNEAVDRFLMPSDS
HHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
GLLDWPLLKFSEHSSFYWLHGQPVRAPDAPKFGMVRVQDHEGRFIGIGEVAEDGRIAPRR
CCCCCCHHEECCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCHHH
LIRSE
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA