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The map label for this gene is pnp [H]

Identifier: 66047403

GI number: 66047403

Start: 4967358

End: 4969463

Strand: Reverse

Name: pnp [H]

Synonym: Psyr_4176

Alternate gene names: 66047403

Gene position: 4969463-4967358 (Counterclockwise)

Preceding gene: 66047404

Following gene: 66047402

Centisome position: 81.55

GC content: 59.02

Gene sequence:

>2106_bases
GTGAACCCGGTAATCAAAAAGTTTCAGTTCGGTCAATCGACCGTAACCCTCGAGACAGGCCGTATCGCCCGTCAAGCCTC
TGGTGCAGTATTGGTCACCGTTGACGACGACGTCAGCGTACTCGTGACTGTGGTCGGCGCCAAACAGGCTGACGCTGGCA
AAGGCTTCTTCCCTCTGTCCGTGCACTATCAGGAAAAAACCTACGCTGCCGGCAAGATTCCTGGTGGTTTCTTCAAGCGT
GAAGGCCGTCCTTCCGAGAAAGAAACCCTGACTTCGCGCCTGATCGACCGTCCGATCCGCCCGTTGTTTCCAGAAGGCTT
CATGAACGAAGTGCAGGTCGTCTGCACCGTCGTCTCCACCAGCAAGAAAATCGATCCGGACATCGCTGCGATGATCGGTA
CTTCGGCTGCCCTGGCCATCTCCGGTATCCCGTTTGACGGTCCGGTCGGTGCTGCCCGCGTTGCTTTCCACGAAAGCACC
GGCTACCTGTTGAACCCGACTTACGAGCAGCTGCAGGCTTCGAGCCTGGACATGGTCGTTGCCGGTACTTCCGAAGCCGT
TCTGATGGTCGAGTCGGAAGCCAAAGAGCTGACCGAAGACCAGATGCTGGGCGCCGTGCTGTTCGCGCATGACGAGTTCC
AGGTCGTGATCAACGCCATCAAGGAACTGGCTGCCGAAGCCGCCAAGCCTACCTGGGACTGGCAGCCAAAGCCTGAAGCG
ACTGCACTGCTGGGCGCTATCCGTGCCGAGTTCGGCGACGCGATCTCCCAGGCTTACACCATCACCGTCAAAGCCGACCG
CTATGCGCGTCTGGGCGAACTGAAAGATCAGGTCGTTGCCAAGCTCGCTGTCGAAGACGGCAGCCCGTCTGCCAGCGAAG
TCAAAGCGGCTTTCGGCGAAATCGAATACCGCACCGTTCGCGAAAACATCGTTAACGGCAAGCCGCGTATCGATGGTCGT
GACACGCGCACCGTTCGTCCTCTGAACATCGAAGTCGGCGTTCTGCCGAAGACTCACGGTTCGGCACTGTTCACCCGTGG
CGAAACTCAGGCGCTGGTGGTTGCCACGCTGGGTACTGCACGTGACGCACAGCTGCTCGATACCCTCGAAGGCGAGAAGA
AAGACCCCTTCATGCTGCACTACAACTTCCCTCCGTTCTCGGTGGGCGAGTGTGGTCGCATGGGCGGCGCAGGTCGTCGT
GAAATCGGTCACGGCCGTCTGGCCCGTCGCAGTGTCCAGGCCATGCTGCCAGGCGCTGACGTGTTCCCGTACACCATCCG
TGTCGTGTCGGAAATCACCGAGTCCAACGGCTCCAGCTCCATGGCTTCGGTCTGCGGTGCTTCCCTGGCTCTGATGGACG
CTGGTGTGCCGATGAAGGCGCCGGTTGCCGGTATCGCCATGGGTCTGGTCAAGGAAGGCGAGAAGTTCGCGATTCTGACC
GACATCCTGGGTGACGAAGACCACCTGGGCGACATGGACTTCAAGGTAGCCGGTACCTCCAAAGGTGTTACCGCGCTGCA
GATGGACATCAAGATCAAAGGCATCACCGAAGAGATCATGGAGATCGCCCTGGGCCAGGCCCTGGAAGCTCGCCTGAATA
TCCTCGGCCAGATGAACCAGATCATTGGTCAGTCGCGTAACGAACTGTCGGCCAACGCTCCGACCATGATCGCGATGAAG
ATCGACACCGACAAGATCCGTGACGTGATCGGCAAGGGTGGCGCAACCATCCGTGCCATCTGCGAAGAGACCAAGGCTTC
GATCGACATCGAAGACGACGGCTCGATCAAGATCTTCGGCGAGTCCAAGGAAGCGGCTGAGGCTGCACGTCAGCGCGTTC
TGGGCATCACCGCAGAAGCGGAAATCGGCAAGATCTACATCGGCAAGGTTGAGCGCATCGTCGATTTCGGCGCGTTCGTC
AACATCCTGCCGGGTAAGGACGGTCTGGTTCATATCTCGATGCTGAGCGATGCTCGCGTAGAGAAAGTGACCGACATCCT
CAAAGAAGGTCAGGAAGTCGAAGTACTGGTACTGGACGTGGACAACCGCGGCCGTATCAAGCTGTCGATCAAGGACGTAG
CCGCAGCGAAAGCGTCGGGCGTCTGA

Upstream 100 bases:

>100_bases
TCAAGTTTTATCTGGGCTGTGGAAGGGGCCGATTCCCCACGCTGCCCAAGAATTCGCAAGAAACCAGTTCCCCAAGAGCC
ACAAAGAAAGGTAGGAAACC

Downstream 100 bases:

>100_bases
TTGAGCTGAGCGGCATGTGAAAAAGGACTCTTCGGAGTCCTTTTTTTATTAGCGCAATTAAAGGCTTACACATGGAGCAT
GCAATCTGCTCGGTGCAAAC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MNPVIKKFQFGQSTVTLETGRIARQASGAVLVTVDDDVSVLVTVVGAKQADAGKGFFPLSVHYQEKTYAAGKIPGGFFKR
EGRPSEKETLTSRLIDRPIRPLFPEGFMNEVQVVCTVVSTSKKIDPDIAAMIGTSAALAISGIPFDGPVGAARVAFHEST
GYLLNPTYEQLQASSLDMVVAGTSEAVLMVESEAKELTEDQMLGAVLFAHDEFQVVINAIKELAAEAAKPTWDWQPKPEA
TALLGAIRAEFGDAISQAYTITVKADRYARLGELKDQVVAKLAVEDGSPSASEVKAAFGEIEYRTVRENIVNGKPRIDGR
DTRTVRPLNIEVGVLPKTHGSALFTRGETQALVVATLGTARDAQLLDTLEGEKKDPFMLHYNFPPFSVGECGRMGGAGRR
EIGHGRLARRSVQAMLPGADVFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPMKAPVAGIAMGLVKEGEKFAILT
DILGDEDHLGDMDFKVAGTSKGVTALQMDIKIKGITEEIMEIALGQALEARLNILGQMNQIIGQSRNELSANAPTMIAMK
IDTDKIRDVIGKGGATIRAICEETKASIDIEDDGSIKIFGESKEAAEAARQRVLGITAEAEIGKIYIGKVERIVDFGAFV
NILPGKDGLVHISMLSDARVEKVTDILKEGQEVEVLVLDVDNRGRIKLSIKDVAAAKASGV

Sequences:

>Translated_701_residues
MNPVIKKFQFGQSTVTLETGRIARQASGAVLVTVDDDVSVLVTVVGAKQADAGKGFFPLSVHYQEKTYAAGKIPGGFFKR
EGRPSEKETLTSRLIDRPIRPLFPEGFMNEVQVVCTVVSTSKKIDPDIAAMIGTSAALAISGIPFDGPVGAARVAFHEST
GYLLNPTYEQLQASSLDMVVAGTSEAVLMVESEAKELTEDQMLGAVLFAHDEFQVVINAIKELAAEAAKPTWDWQPKPEA
TALLGAIRAEFGDAISQAYTITVKADRYARLGELKDQVVAKLAVEDGSPSASEVKAAFGEIEYRTVRENIVNGKPRIDGR
DTRTVRPLNIEVGVLPKTHGSALFTRGETQALVVATLGTARDAQLLDTLEGEKKDPFMLHYNFPPFSVGECGRMGGAGRR
EIGHGRLARRSVQAMLPGADVFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPMKAPVAGIAMGLVKEGEKFAILT
DILGDEDHLGDMDFKVAGTSKGVTALQMDIKIKGITEEIMEIALGQALEARLNILGQMNQIIGQSRNELSANAPTMIAMK
IDTDKIRDVIGKGGATIRAICEETKASIDIEDDGSIKIFGESKEAAEAARQRVLGITAEAEIGKIYIGKVERIVDFGAFV
NILPGKDGLVHISMLSDARVEKVTDILKEGQEVEVLVLDVDNRGRIKLSIKDVAAAKASGV
>Mature_701_residues
MNPVIKKFQFGQSTVTLETGRIARQASGAVLVTVDDDVSVLVTVVGAKQADAGKGFFPLSVHYQEKTYAAGKIPGGFFKR
EGRPSEKETLTSRLIDRPIRPLFPEGFMNEVQVVCTVVSTSKKIDPDIAAMIGTSAALAISGIPFDGPVGAARVAFHEST
GYLLNPTYEQLQASSLDMVVAGTSEAVLMVESEAKELTEDQMLGAVLFAHDEFQVVINAIKELAAEAAKPTWDWQPKPEA
TALLGAIRAEFGDAISQAYTITVKADRYARLGELKDQVVAKLAVEDGSPSASEVKAAFGEIEYRTVRENIVNGKPRIDGR
DTRTVRPLNIEVGVLPKTHGSALFTRGETQALVVATLGTARDAQLLDTLEGEKKDPFMLHYNFPPFSVGECGRMGGAGRR
EIGHGRLARRSVQAMLPGADVFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPMKAPVAGIAMGLVKEGEKFAILT
DILGDEDHLGDMDFKVAGTSKGVTALQMDIKIKGITEEIMEIALGQALEARLNILGQMNQIIGQSRNELSANAPTMIAMK
IDTDKIRDVIGKGGATIRAICEETKASIDIEDDGSIKIFGESKEAAEAARQRVLGITAEAEIGKIYIGKVERIVDFGAFV
NILPGKDGLVHISMLSDARVEKVTDILKEGQEVEVLVLDVDNRGRIKLSIKDVAAAKASGV

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=714, Percent_Identity=35.8543417366947, Blast_Score=414, Evalue=1e-115,
Organism=Escherichia coli, GI145693187, Length=692, Percent_Identity=63.0057803468208, Blast_Score=882, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=713, Percent_Identity=33.8008415147265, Blast_Score=337, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI17535281, Length=77, Percent_Identity=48.0519480519481, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320850, Length=103, Percent_Identity=35.9223300970874, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281362905, Length=703, Percent_Identity=36.8421052631579, Blast_Score=423, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24651641, Length=703, Percent_Identity=36.8421052631579, Blast_Score=423, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24651643, Length=703, Percent_Identity=36.8421052631579, Blast_Score=423, Evalue=1e-118,
Organism=Drosophila melanogaster, GI161079377, Length=650, Percent_Identity=36.7692307692308, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 74943; Mature: 74943

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVIKKFQFGQSTVTLETGRIARQASGAVLVTVDDDVSVLVTVVGAKQADAGKGFFPLS
CCCHHHHHCCCCCEEEEECCCHHHHCCCEEEEEECCCCEEEEEEECCCCCCCCCCEEEEE
VHYQEKTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPIRPLFPEGFMNEVQVVCTVVST
EEECCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHEEEEEECC
SKKIDPDIAAMIGTSAALAISGIPFDGPVGAARVAFHESTGYLLNPTYEQLQASSLDMVV
CCCCCCHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCEECCCHHHHHCCCCCEEE
AGTSEAVLMVESEAKELTEDQMLGAVLFAHDEFQVVINAIKELAAEAAKPTWDWQPKPEA
ECCCCEEEEEECHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
TALLGAIRAEFGDAISQAYTITVKADRYARLGELKDQVVAKLAVEDGSPSASEVKAAFGE
HHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCC
IEYRTVRENIVNGKPRIDGRDTRTVRPLNIEVGVLPKTHGSALFTRGETQALVVATLGTA
CHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEECCCCCEEEEEECCCC
RDAQLLDTLEGEKKDPFMLHYNFPPFSVGECGRMGGAGRREIGHGRLARRSVQAMLPGAD
CCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
VFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPMKAPVAGIAMGLVKEGEKFAILT
CCHHHHHHHHHHHHCCCCHHHHHHHCCHHEEEECCCCCCCCHHHHHHHHHHCCCCEEEEE
DILGDEDHLGDMDFKVAGTSKGVTALQMDIKIKGITEEIMEIALGQALEARLNILGQMNQ
ECCCCCCCCCCCEEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGQSRNELSANAPTMIAMKIDTDKIRDVIGKGGATIRAICEETKASIDIEDDGSIKIFG
HHCCCCCCCCCCCCEEEEEEECHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCCEEEEE
ESKEAAEAARQRVLGITAEAEIGKIYIGKVERIVDFGAFVNILPGKDGLVHISMLSDARV
CCHHHHHHHHHHHEEEEECCCCCEEEEHHHHHHHHHCCEEEEECCCCCEEEEEECCCHHH
EKVTDILKEGQEVEVLVLDVDNRGRIKLSIKDVAAAKASGV
HHHHHHHHCCCCEEEEEEEECCCCEEEEEEHHHHHHHCCCC
>Mature Secondary Structure
MNPVIKKFQFGQSTVTLETGRIARQASGAVLVTVDDDVSVLVTVVGAKQADAGKGFFPLS
CCCHHHHHCCCCCEEEEECCCHHHHCCCEEEEEECCCCEEEEEEECCCCCCCCCCEEEEE
VHYQEKTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPIRPLFPEGFMNEVQVVCTVVST
EEECCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHEEEEEECC
SKKIDPDIAAMIGTSAALAISGIPFDGPVGAARVAFHESTGYLLNPTYEQLQASSLDMVV
CCCCCCHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCEECCCHHHHHCCCCCEEE
AGTSEAVLMVESEAKELTEDQMLGAVLFAHDEFQVVINAIKELAAEAAKPTWDWQPKPEA
ECCCCEEEEEECHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
TALLGAIRAEFGDAISQAYTITVKADRYARLGELKDQVVAKLAVEDGSPSASEVKAAFGE
HHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCC
IEYRTVRENIVNGKPRIDGRDTRTVRPLNIEVGVLPKTHGSALFTRGETQALVVATLGTA
CHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEECCCCCEEEEEECCCC
RDAQLLDTLEGEKKDPFMLHYNFPPFSVGECGRMGGAGRREIGHGRLARRSVQAMLPGAD
CCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
VFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPMKAPVAGIAMGLVKEGEKFAILT
CCHHHHHHHHHHHHCCCCHHHHHHHCCHHEEEECCCCCCCCHHHHHHHHHHCCCCEEEEE
DILGDEDHLGDMDFKVAGTSKGVTALQMDIKIKGITEEIMEIALGQALEARLNILGQMNQ
ECCCCCCCCCCCEEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGQSRNELSANAPTMIAMKIDTDKIRDVIGKGGATIRAICEETKASIDIEDDGSIKIFG
HHCCCCCCCCCCCCEEEEEEECHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCCEEEEE
ESKEAAEAARQRVLGITAEAEIGKIYIGKVERIVDFGAFVNILPGKDGLVHISMLSDARV
CCHHHHHHHHHHHEEEEECCCCCEEEEHHHHHHHHHCCEEEEECCCCCEEEEEECCCHHH
EKVTDILKEGQEVEVLVLDVDNRGRIKLSIKDVAAAKASGV
HHHHHHHHCCCCEEEEEEEECCCCEEEEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA