Click here to switch to the map view.

The map label for this gene is ptsN [H]

Identifier: 66047376

GI number: 66047376

Start: 4936465

End: 4936929

Strand: Direct

Name: ptsN [H]

Synonym: Psyr_4149

Alternate gene names: 66047376

Gene position: 4936465-4936929 (Clockwise)

Preceding gene: 66047375

Following gene: 66047377

Centisome position: 81.01

GC content: 59.35

Gene sequence:

>465_bases
ATGATCCGAATCGAAAATATCCTGACCCCCGGCCGCTCCCTTGTGAACGTGCCGGGCGGCAGTAAAAAGCGTGTACTCGA
AGAAATTGCCAACCTGATCGGCCGTGAAGTGCCGGGCATGGTGTCGGACACAGTATTCGCAAGTCTCGTCGCCCGGGAAA
AACTGGGCTCGACCGGTTTTGGCAACGGTATTGCCATCCCCCACTGCCGCCTCGCTGACTGCAGCGAGCCTGTCAGTGCA
GTGATTCACCTCTCCACCCCTGTGGATTTCGACGCCATCGATGGTGCGCCGGTCGACCTGCTGTTCGTCTTGCTGGTCCC
GCAAGCTGCCACCGATGAACACCTGGAGCTGCTGCGCCAGATCGCCAGCATGCTCGACGACAAAGATGTGCGTAAGCGCC
TGCGCAGTGCCGAAAGCGCTGAGGCGCTTTATCAAGTGGTGCTGGACGTGCAGAACGGAAACTGA

Upstream 100 bases:

>100_bases
ATCGACCTGCTCACTGACAAGCTAGACCGACAATTGCTCAAGCATAAGGAAAAGCAGCAAAGCATCCTCAAAGGTGCGGC
CGCTCGCTAACGCCCATTTA

Downstream 100 bases:

>100_bases
CCATGCGCATGATCATCGTCAGCGGTCGCTCCGGCTCAGGCAAGAGTACGGCGCTCGATGTGCTGGAAGACAACGGCTTT
TACTGTGTCGATAACCTGCC

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MIRIENILTPGRSLVNVPGGSKKRVLEEIANLIGREVPGMVSDTVFASLVAREKLGSTGFGNGIAIPHCRLADCSEPVSA
VIHLSTPVDFDAIDGAPVDLLFVLLVPQAATDEHLELLRQIASMLDDKDVRKRLRSAESAEALYQVVLDVQNGN

Sequences:

>Translated_154_residues
MIRIENILTPGRSLVNVPGGSKKRVLEEIANLIGREVPGMVSDTVFASLVAREKLGSTGFGNGIAIPHCRLADCSEPVSA
VIHLSTPVDFDAIDGAPVDLLFVLLVPQAATDEHLELLRQIASMLDDKDVRKRLRSAESAEALYQVVLDVQNGN
>Mature_154_residues
MIRIENILTPGRSLVNVPGGSKKRVLEEIANLIGREVPGMVSDTVFASLVAREKLGSTGFGNGIAIPHCRLADCSEPVSA
VIHLSTPVDFDAIDGAPVDLLFVLLVPQAATDEHLELLRQIASMLDDKDVRKRLRSAESAEALYQVVLDVQNGN

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=132, Percent_Identity=48.4848484848485, Blast_Score=121, Evalue=2e-29,
Organism=Escherichia coli, GI1786951, Length=100, Percent_Identity=36, Blast_Score=67, Evalue=5e-13,
Organism=Escherichia coli, GI2367327, Length=131, Percent_Identity=25.9541984732824, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16568; Mature: 16568

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIENILTPGRSLVNVPGGSKKRVLEEIANLIGREVPGMVSDTVFASLVAREKLGSTGF
CEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
GNGIAIPHCRLADCSEPVSAVIHLSTPVDFDAIDGAPVDLLFVLLVPQAATDEHLELLRQ
CCCCCCCCCCCCCCCHHHHHEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
IASMLDDKDVRKRLRSAESAEALYQVVLDVQNGN
HHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRIENILTPGRSLVNVPGGSKKRVLEEIANLIGREVPGMVSDTVFASLVAREKLGSTGF
CEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
GNGIAIPHCRLADCSEPVSAVIHLSTPVDFDAIDGAPVDLLFVLLVPQAATDEHLELLRQ
CCCCCCCCCCCCCCCHHHHHEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
IASMLDDKDVRKRLRSAESAEALYQVVLDVQNGN
HHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]