Click here to switch to the map view.

The map label for this gene is mraW [H]

Identifier: 66047337

GI number: 66047337

Start: 4900478

End: 4901419

Strand: Reverse

Name: mraW [H]

Synonym: Psyr_4110

Alternate gene names: 66047337

Gene position: 4901419-4900478 (Counterclockwise)

Preceding gene: 66047338

Following gene: 66047336

Centisome position: 80.43

GC content: 60.4

Gene sequence:

>942_bases
ATGAATAGCGGCTTTACCCACATCACCGTGCTGCTTGAAGAAGCCGTGGAGGCTCTCGCGGTGCGCGCCGATGGCTGCTA
TCTGGACGGCACGTTCGGACGTGGCGGGCATAGTCGTCTGATACTCAGCCAGCTGGGCCCCGATGGTCGGCTTCTGGGAT
TCGACAAGGACCCTCAAGCGATTGCCACCGGGCAAGCGCTAGCGGCCGAAGACGGCCGCTTTGTCATTGTGCAGCGCAGC
TTTGCCGAATTGGGATCCGAAACTCAGGAACGCGGTTTGGCCGGCAAGGTCAGTGGCATCCTGCTGGATCTGGGTGTGTC
GTCCCCGCAGCTGGATGATCCCGAGCGCGGTTTCAGCTTCATGAACGACGGTCCTCTGGACATGCGCATGGACCCCGCCC
GTGGCATAAGCGCCGCCGAGTTCATCGCGACTGCACCGGCTGAAGAAATTGCCCGCGTCTTCAAGGAATACGGCGAAGAG
CGGTTTGCCAAGCGGATGGCCAATGCGGTCGTTGCGCGTCGCGAAGTCCAGCCTTTCGAGCGCACAGCCGATCTGGCAGA
AGTGCTCAAGGTCGCCAACCCTGCCTGGGAAAAGGGCAAGAATCCGGCAACGCGTGCCTTTCAGGGCCTGCGTATTCACG
TAAACAACGAACTGGGCGATCTGGAAGCCGGCCTCGAAGCGGCACTGGATGCGCTGGAAGTGGGTGGCCGCCTGGTCGTC
ATCAGCTTTCACTCGCTGGAAGACCGCATCGTCAAACTGTTCATGCGCAAGCTCGCCAAGGGCGAAGCCGACAACATGCC
GCGCAATCTGCCGATCCAGTTCAAACCGTTCGAACCGAAAATCAAAATTCACGGCAAGGCTCAATTCGCCTCGGATGCCG
AAACCAAAGCCAACCCGCGCTCGCGCAGTGCTGTCATGCGTGTAGCGGAGAAGCTCAGGTGA

Upstream 100 bases:

>100_bases
GGGACGAGGACGCCTGGAACGCTCTTGCTGATGCTGACCTGGCTGCCATTCAAAAACCTGGCGCGATGCCCGACGAATTA
CGTGATTTGATCCTGTGACT

Downstream 100 bases:

>100_bases
GCCGTCTGTTTCTGAAGCCTCTGCCCGGCGGCAGTTTCATTATGCTGCTGCTGTTCATTGCTGTCCTGATTTCGGCGATT
GGCGTTTCCTATAGTGCACA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNSGFTHITVLLEEAVEALAVRADGCYLDGTFGRGGHSRLILSQLGPDGRLLGFDKDPQAIATGQALAAEDGRFVIVQRS
FAELGSETQERGLAGKVSGILLDLGVSSPQLDDPERGFSFMNDGPLDMRMDPARGISAAEFIATAPAEEIARVFKEYGEE
RFAKRMANAVVARREVQPFERTADLAEVLKVANPAWEKGKNPATRAFQGLRIHVNNELGDLEAGLEAALDALEVGGRLVV
ISFHSLEDRIVKLFMRKLAKGEADNMPRNLPIQFKPFEPKIKIHGKAQFASDAETKANPRSRSAVMRVAEKLR

Sequences:

>Translated_313_residues
MNSGFTHITVLLEEAVEALAVRADGCYLDGTFGRGGHSRLILSQLGPDGRLLGFDKDPQAIATGQALAAEDGRFVIVQRS
FAELGSETQERGLAGKVSGILLDLGVSSPQLDDPERGFSFMNDGPLDMRMDPARGISAAEFIATAPAEEIARVFKEYGEE
RFAKRMANAVVARREVQPFERTADLAEVLKVANPAWEKGKNPATRAFQGLRIHVNNELGDLEAGLEAALDALEVGGRLVV
ISFHSLEDRIVKLFMRKLAKGEADNMPRNLPIQFKPFEPKIKIHGKAQFASDAETKANPRSRSAVMRVAEKLR
>Mature_313_residues
MNSGFTHITVLLEEAVEALAVRADGCYLDGTFGRGGHSRLILSQLGPDGRLLGFDKDPQAIATGQALAAEDGRFVIVQRS
FAELGSETQERGLAGKVSGILLDLGVSSPQLDDPERGFSFMNDGPLDMRMDPARGISAAEFIATAPAEEIARVFKEYGEE
RFAKRMANAVVARREVQPFERTADLAEVLKVANPAWEKGKNPATRAFQGLRIHVNNELGDLEAGLEAALDALEVGGRLVV
ISFHSLEDRIVKLFMRKLAKGEADNMPRNLPIQFKPFEPKIKIHGKAQFASDAETKANPRSRSAVMRVAEKLR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=348, Percent_Identity=37.0689655172414, Blast_Score=194, Evalue=6e-50,
Organism=Homo sapiens, GI165377202, Length=193, Percent_Identity=38.860103626943, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI1786270, Length=318, Percent_Identity=55.6603773584906, Blast_Score=335, Evalue=3e-93,
Organism=Drosophila melanogaster, GI62472493, Length=329, Percent_Identity=35.8662613981763, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28571637, Length=329, Percent_Identity=35.8662613981763, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 34151; Mature: 34151

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSGFTHITVLLEEAVEALAVRADGCYLDGTFGRGGHSRLILSQLGPDGRLLGFDKDPQA
CCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEHHHCCCCCCEECCCCCCCH
IATGQALAAEDGRFVIVQRSFAELGSETQERGLAGKVSGILLDLGVSSPQLDDPERGFSF
HHCCCCEECCCCCEEEEECHHHHHCCHHHHCCCCHHHCEEEEECCCCCCCCCCHHHCCCC
MNDGPLDMRMDPARGISAAEFIATAPAEEIARVFKEYGEERFAKRMANAVVARREVQPFE
CCCCCCCEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
RTADLAEVLKVANPAWEKGKNPATRAFQGLRIHVNNELGDLEAGLEAALDALEVGGRLVV
HHHHHHHHHHHCCCHHHCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEE
ISFHSLEDRIVKLFMRKLAKGEADNMPRNLPIQFKPFEPKIKIHGKAQFASDAETKANPR
EEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCCCCCCCCCH
SRSAVMRVAEKLR
HHHHHHHHHHHCC
>Mature Secondary Structure
MNSGFTHITVLLEEAVEALAVRADGCYLDGTFGRGGHSRLILSQLGPDGRLLGFDKDPQA
CCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEHHHCCCCCCEECCCCCCCH
IATGQALAAEDGRFVIVQRSFAELGSETQERGLAGKVSGILLDLGVSSPQLDDPERGFSF
HHCCCCEECCCCCEEEEECHHHHHCCHHHHCCCCHHHCEEEEECCCCCCCCCCHHHCCCC
MNDGPLDMRMDPARGISAAEFIATAPAEEIARVFKEYGEERFAKRMANAVVARREVQPFE
CCCCCCCEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
RTADLAEVLKVANPAWEKGKNPATRAFQGLRIHVNNELGDLEAGLEAALDALEVGGRLVV
HHHHHHHHHHHCCCHHHCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEE
ISFHSLEDRIVKLFMRKLAKGEADNMPRNLPIQFKPFEPKIKIHGKAQFASDAETKANPR
EEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCCCCCCCCCH
SRSAVMRVAEKLR
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA