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The map label for this gene is murD [H]

Identifier: 66047331

GI number: 66047331

Start: 4893201

End: 4894547

Strand: Reverse

Name: murD [H]

Synonym: Psyr_4104

Alternate gene names: 66047331

Gene position: 4894547-4893201 (Counterclockwise)

Preceding gene: 66047332

Following gene: 66047330

Centisome position: 80.32

GC content: 62.81

Gene sequence:

>1347_bases
GTGTCCCTGATCGTTTCCGACCGCTTCCGCATTGTCGTGGGTCTAGGCAAAAGCGGCATGTCCCTGGTTCGCTTCCTGGC
GAACCAGGGCGTGTCCTTTGCCGTGGCCGACACGCGGGAGAATCCTCCTGAGCTGGCGACCTTGCGTCGCGACTATCCGC
AGGTGGAAGTGCGTTGTGGCGAGCTGGACGTGGATTTCCTCTGCCGTGCCGATGAGCTGTATGTCAGCCCCGGTCTGGCC
CTGGCCACCCCGGCCCTGCAGCAGGCGCACGCACGCGGCGTCAAACTGTCGGGTGACATCGAACTGTTCGCGCGTTACGC
GAAGGCGCCGGTGATTGCCATTACCGGCTCGAATGCAAAAAGCACCGTGACCACGCTGGTCGGCGAGATGGCCGCTGCCG
CTGGAAAGCGTGTAGCGGTGGGCGGCAATCTTGGCACTCCGGCGCTGGACCTGCTCAGCGATGAGGTCGAGCTGTACGTG
ATGGAGCTCTCCAGCTTTCAGCTCGAAACCACCGACCAGCTCAATGCTGAAGTGGCGACCGTGCTGAATATCAGCGAAGA
CCATATGGACCGCTACAGCGGCCTGCCTGCCTACCACCTGGCCAAGCACCGGATTTTCCGTGGCGCGCGGCAAGTGGTGG
TCAATGGTCAGGATGCGCTGTCCCGTCCGCTGATTGGCGAAGGCCTGCCGTGCTGGACATTCGGCCTCAACAAGCCCGAC
TTCCATGGTTTCGGCCTGCGTGAAGAGAACGGCGAAAAGTACCTGGCGTTCCAGTTTGAAAACCTCATGCCGGTGCGTGA
GCTGAAAGTGCGCGGTGCGCACAATCAGGCCAACGCGCTTGCCGCCCTGGCGCTCGGGCATGCGGTCGGCCTGCCGTTCG
ACGCCATGCTCGCCAGCCTGCGTGAATTCACCGGCCTGGAGCATCGCTGCCAGTGGCTGCGCGAGCACGATGGCGTTCAT
TACTACAACGATTCCAAAGCCACCAATGTCGGTGCCGCACTGGCTGCCATCGAAGGGCTGGGCTCGGATATCGACGGCAA
GCTGGTGCTGATCGCCGGCGGCGATGGCAAAGGCGCCGATTTCAGCGGTTTGCGTGCGCCAGTCGCCAGGCATTGCCGCG
CGGCGGTACTGCTTGGGCGTGATGCCGAGCTGATTGCCCAGGCATTGGGCGACGCCGTTCCGCTGCTGCGTGTCGACACC
GTCCAGGCGGCTGTCGAGCACAGCGCAAAACTGGCGCAGTGTGGCGACGCCGTATTGCTTTCTCCTGCATGCGCCAGTCT
GGACATGTTCAAGAATTATGAAGAGCGCGGACGTGTGTTCGCGCAGGCAGTGGAGTGCTTGTCATGA

Upstream 100 bases:

>100_bases
TCAAAGGCTGGCCCGAGCCACGTGTGATCGTCCGTTTCTGGATCATCACCGTGATTCTGGTGCTCATTGGCCTTGCCACG
CTGAAACTGAGGTAGAACGA

Downstream 100 bases:

>100_bases
TCTTCGGTGTGATCAAGCCTTATCCATCGCCACTGATCAGCGGGCGCGGCATCGACCTCGATTTCCCGATGCTGGTCGGC
TGCCTGGCGCTGCTCGGCCT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSLIVSDRFRIVVGLGKSGMSLVRFLANQGVSFAVADTRENPPELATLRRDYPQVEVRCGELDVDFLCRADELYVSPGLA
LATPALQQAHARGVKLSGDIELFARYAKAPVIAITGSNAKSTVTTLVGEMAAAAGKRVAVGGNLGTPALDLLSDEVELYV
MELSSFQLETTDQLNAEVATVLNISEDHMDRYSGLPAYHLAKHRIFRGARQVVVNGQDALSRPLIGEGLPCWTFGLNKPD
FHGFGLREENGEKYLAFQFENLMPVRELKVRGAHNQANALAALALGHAVGLPFDAMLASLREFTGLEHRCQWLREHDGVH
YYNDSKATNVGAALAAIEGLGSDIDGKLVLIAGGDGKGADFSGLRAPVARHCRAAVLLGRDAELIAQALGDAVPLLRVDT
VQAAVEHSAKLAQCGDAVLLSPACASLDMFKNYEERGRVFAQAVECLS

Sequences:

>Translated_448_residues
MSLIVSDRFRIVVGLGKSGMSLVRFLANQGVSFAVADTRENPPELATLRRDYPQVEVRCGELDVDFLCRADELYVSPGLA
LATPALQQAHARGVKLSGDIELFARYAKAPVIAITGSNAKSTVTTLVGEMAAAAGKRVAVGGNLGTPALDLLSDEVELYV
MELSSFQLETTDQLNAEVATVLNISEDHMDRYSGLPAYHLAKHRIFRGARQVVVNGQDALSRPLIGEGLPCWTFGLNKPD
FHGFGLREENGEKYLAFQFENLMPVRELKVRGAHNQANALAALALGHAVGLPFDAMLASLREFTGLEHRCQWLREHDGVH
YYNDSKATNVGAALAAIEGLGSDIDGKLVLIAGGDGKGADFSGLRAPVARHCRAAVLLGRDAELIAQALGDAVPLLRVDT
VQAAVEHSAKLAQCGDAVLLSPACASLDMFKNYEERGRVFAQAVECLS
>Mature_447_residues
SLIVSDRFRIVVGLGKSGMSLVRFLANQGVSFAVADTRENPPELATLRRDYPQVEVRCGELDVDFLCRADELYVSPGLAL
ATPALQQAHARGVKLSGDIELFARYAKAPVIAITGSNAKSTVTTLVGEMAAAAGKRVAVGGNLGTPALDLLSDEVELYVM
ELSSFQLETTDQLNAEVATVLNISEDHMDRYSGLPAYHLAKHRIFRGARQVVVNGQDALSRPLIGEGLPCWTFGLNKPDF
HGFGLREENGEKYLAFQFENLMPVRELKVRGAHNQANALAALALGHAVGLPFDAMLASLREFTGLEHRCQWLREHDGVHY
YNDSKATNVGAALAAIEGLGSDIDGKLVLIAGGDGKGADFSGLRAPVARHCRAAVLLGRDAELIAQALGDAVPLLRVDTV
QAAVEHSAKLAQCGDAVLLSPACASLDMFKNYEERGRVFAQAVECLS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=438, Percent_Identity=50.4566210045662, Blast_Score=364, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48046; Mature: 47915

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIVSDRFRIVVGLGKSGMSLVRFLANQGVSFAVADTRENPPELATLRRDYPQVEVRCG
CCEEECCCEEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCCEEEEEC
ELDVDFLCRADELYVSPGLALATPALQQAHARGVKLSGDIELFARYAKAPVIAITGSNAK
CCCHHHEECCCCEEECCCHHHHHHHHHHHHHCCCEECCCHHHHHHHHCCCEEEEECCCCH
STVTTLVGEMAAAAGKRVAVGGNLGTPALDLLSDEVELYVMELSSFQLETTDQLNAEVAT
HHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCEEEEEECCCCEECCCHHCCCEEEE
VLNISEDHMDRYSGLPAYHLAKHRIFRGARQVVVNGQDALSRPLIGEGLPCWTFGLNKPD
EEECCHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCHHHHCCCCCCCCCCEEEECCCCCC
FHGFGLREENGEKYLAFQFENLMPVRELKVRGAHNQANALAALALGHAVGLPFDAMLASL
CCCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
REFTGLEHRCQWLREHDGVHYYNDSKATNVGAALAAIEGLGSDIDGKLVLIAGGDGKGAD
HHHHCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
FSGLRAPVARHCRAAVLLGRDAELIAQALGDAVPLLRVDTVQAAVEHSAKLAQCGDAVLL
CCCHHHHHHHHHHHHEEECCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEE
SPACASLDMFKNYEERGRVFAQAVECLS
CCHHHHHHHHHCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLIVSDRFRIVVGLGKSGMSLVRFLANQGVSFAVADTRENPPELATLRRDYPQVEVRCG
CEEECCCEEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCCEEEEEC
ELDVDFLCRADELYVSPGLALATPALQQAHARGVKLSGDIELFARYAKAPVIAITGSNAK
CCCHHHEECCCCEEECCCHHHHHHHHHHHHHCCCEECCCHHHHHHHHCCCEEEEECCCCH
STVTTLVGEMAAAAGKRVAVGGNLGTPALDLLSDEVELYVMELSSFQLETTDQLNAEVAT
HHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCEEEEEECCCCEECCCHHCCCEEEE
VLNISEDHMDRYSGLPAYHLAKHRIFRGARQVVVNGQDALSRPLIGEGLPCWTFGLNKPD
EEECCHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCHHHHCCCCCCCCCCEEEECCCCCC
FHGFGLREENGEKYLAFQFENLMPVRELKVRGAHNQANALAALALGHAVGLPFDAMLASL
CCCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
REFTGLEHRCQWLREHDGVHYYNDSKATNVGAALAAIEGLGSDIDGKLVLIAGGDGKGAD
HHHHCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
FSGLRAPVARHCRAAVLLGRDAELIAQALGDAVPLLRVDTVQAAVEHSAKLAQCGDAVLL
CCCHHHHHHHHHHHHEEECCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEE
SPACASLDMFKNYEERGRVFAQAVECLS
CCHHHHHHHHHCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA