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The map label for this gene is cls [H]

Identifier: 66047271

GI number: 66047271

Start: 4828329

End: 4829486

Strand: Reverse

Name: cls [H]

Synonym: Psyr_4044

Alternate gene names: 66047271

Gene position: 4829486-4828329 (Counterclockwise)

Preceding gene: 66047272

Following gene: 66047260

Centisome position: 79.25

GC content: 63.47

Gene sequence:

>1158_bases
ATGAACGGTGCGATTTTCCCGTGGCGTGAAAACAACCGCTTTCAATTGCTGATCGACGGCCCTGCGTTCTTTCCGCGCAT
GATCGCAGCCATTGATCGCGCCGAGCAGCAGGTCGATCTGGAGTTGTACCTGGTCGAAGCCGGGGCTTGCGCCGACGCCA
TCGTGCGCGCGCTGGTCGAGGCGGGCAGACGTGGCGTGATCGTGCGCTGCCTGTTCGATGACTTCGGTTCAAAAGCCTTC
GACGCCGGCCTGCGCAAGCAACTGACCGATGCCGGTGTGATTCTGCGTTTCTACAATCCGATCCACTGGCGGCGCGGCGT
GCGCAACTTCTACCGCGACCATCGCAAACTGCTGGTGGTGGACAAGGCCTTGGCCGTGGTGGGCGGCACCGGCGTGACCG
ACGAGTTCTGGGAGCCGGACAAGGACGCCAGCCAGTGGCACGAAGTGATGGTCGAAATCACCGGGCCGCTGGTGATTGAC
TGGCAGGCGCTGTTCAACCGGCAGTTCCACGCCAACGCCCGGCGTTTCGCCTGGCGTCCGAAAGAAAACTTCGGCCTTGA
TCACCTGCCCAAGGCCCCGACAACCGGCGAGGGGCTGGGCCGCGTGGCGTATGCCGATTCCCGCCAGCACCGCGACATTC
TGCAATCGCTGGTGCGTGCGCTGCACACCGGGCAAAAGCGGATCTGGCTGGCAACCCCTTACTTTCTGCCCACCTGGAAA
GTCCGCCGCGCCTTGCGCCGGGCCGCGGCGCGTGGCGTTGATGTGCGTTTGCTGCTGACCGGGCCGCGCACCGATCACCC
GTCCGTACGCTATGCCGGTCAACGCTACTACCCGCGCCTGTTGCGCGCCGGGGTCAAGGTGTTCGAATACCAGCCCTGCT
TTCTGCACCTGAAAATGGTCCTGATCGACGACTGGGTGAGTCTCGGCTCGTGCAACTTCGATCACTGGAACCTGCGCTTC
AACCTGGAAGCCAACCTCGAAGCCCTCGACCCCGGACTGACCCGCGACGTGGTCGCCAGCTTCGAAAAAGACTTCGCCCT
GAGCGCCGAAACCACGTTGGCAGACTGGAAGGCCAGACCGCTGTGGCGACGGGTCAAGCAGCGCTTGTGGGGCTGGATCG
ACCGGCTGGTGGTGAACTTGCTGGATCAGCGTAACTGA

Upstream 100 bases:

>100_bases
ATGGCCCCGGACTTCAAAGCCTTGCGCAATGCCGCAGGATTGTCGGCGGTGAGCCTCTCGGTCCCGGTCGGGGCTGTCCT
GATCTTCACGGCGCGCTGAT

Downstream 100 bases:

>100_bases
GGTTTGCGCGGGGCCGAGCGCGGCCCCGGTTGTCATCTGCTCAAACGATGCATTCCAGTGAACGATCAACCATGGCTTTG
GCCATCTCGGCCATGTGCAA

Product: phospholipase D/transphosphatidylase

Products: cardiolipin; glycerol [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MNGAIFPWRENNRFQLLIDGPAFFPRMIAAIDRAEQQVDLELYLVEAGACADAIVRALVEAGRRGVIVRCLFDDFGSKAF
DAGLRKQLTDAGVILRFYNPIHWRRGVRNFYRDHRKLLVVDKALAVVGGTGVTDEFWEPDKDASQWHEVMVEITGPLVID
WQALFNRQFHANARRFAWRPKENFGLDHLPKAPTTGEGLGRVAYADSRQHRDILQSLVRALHTGQKRIWLATPYFLPTWK
VRRALRRAAARGVDVRLLLTGPRTDHPSVRYAGQRYYPRLLRAGVKVFEYQPCFLHLKMVLIDDWVSLGSCNFDHWNLRF
NLEANLEALDPGLTRDVVASFEKDFALSAETTLADWKARPLWRRVKQRLWGWIDRLVVNLLDQRN

Sequences:

>Translated_385_residues
MNGAIFPWRENNRFQLLIDGPAFFPRMIAAIDRAEQQVDLELYLVEAGACADAIVRALVEAGRRGVIVRCLFDDFGSKAF
DAGLRKQLTDAGVILRFYNPIHWRRGVRNFYRDHRKLLVVDKALAVVGGTGVTDEFWEPDKDASQWHEVMVEITGPLVID
WQALFNRQFHANARRFAWRPKENFGLDHLPKAPTTGEGLGRVAYADSRQHRDILQSLVRALHTGQKRIWLATPYFLPTWK
VRRALRRAAARGVDVRLLLTGPRTDHPSVRYAGQRYYPRLLRAGVKVFEYQPCFLHLKMVLIDDWVSLGSCNFDHWNLRF
NLEANLEALDPGLTRDVVASFEKDFALSAETTLADWKARPLWRRVKQRLWGWIDRLVVNLLDQRN
>Mature_385_residues
MNGAIFPWRENNRFQLLIDGPAFFPRMIAAIDRAEQQVDLELYLVEAGACADAIVRALVEAGRRGVIVRCLFDDFGSKAF
DAGLRKQLTDAGVILRFYNPIHWRRGVRNFYRDHRKLLVVDKALAVVGGTGVTDEFWEPDKDASQWHEVMVEITGPLVID
WQALFNRQFHANARRFAWRPKENFGLDHLPKAPTTGEGLGRVAYADSRQHRDILQSLVRALHTGQKRIWLATPYFLPTWK
VRRALRRAAARGVDVRLLLTGPRTDHPSVRYAGQRYYPRLLRAGVKVFEYQPCFLHLKMVLIDDWVSLGSCNFDHWNLRF
NLEANLEALDPGLTRDVVASFEKDFALSAETTLADWKARPLWRRVKQRLWGWIDRLVVNLLDQRN

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787007, Length=326, Percent_Identity=34.3558282208589, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1787502, Length=374, Percent_Identity=27.5401069518717, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 44482; Mature: 44482

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGAIFPWRENNRFQLLIDGPAFFPRMIAAIDRAEQQVDLELYLVEAGACADAIVRALVE
CCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHH
AGRRGVIVRCLFDDFGSKAFDAGLRKQLTDAGVILRFYNPIHWRRGVRNFYRDHRKLLVV
CCCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHEEEEE
DKALAVVGGTGVTDEFWEPDKDASQWHEVMVEITGPLVIDWQALFNRQFHANARRFAWRP
EHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHCCHHHCCCHHEECCC
KENFGLDHLPKAPTTGEGLGRVAYADSRQHRDILQSLVRALHTGQKRIWLATPYFLPTWK
CCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHH
VRRALRRAAARGVDVRLLLTGPRTDHPSVRYAGQRYYPRLLRAGVKVFEYQPCFLHLKMV
HHHHHHHHHHCCCEEEEEEECCCCCCCCCEECCCHHHHHHHHCCHHHEECCCHHHHHHHH
LIDDWVSLGSCNFDHWNLRFNLEANLEALDPGLTRDVVASFEKDFALSAETTLADWKARP
HHHHHHHHCCCCCCCEEEEEEEECCCHHCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCH
LWRRVKQRLWGWIDRLVVNLLDQRN
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNGAIFPWRENNRFQLLIDGPAFFPRMIAAIDRAEQQVDLELYLVEAGACADAIVRALVE
CCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHH
AGRRGVIVRCLFDDFGSKAFDAGLRKQLTDAGVILRFYNPIHWRRGVRNFYRDHRKLLVV
CCCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHEEEEE
DKALAVVGGTGVTDEFWEPDKDASQWHEVMVEITGPLVIDWQALFNRQFHANARRFAWRP
EHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHCCHHHCCCHHEECCC
KENFGLDHLPKAPTTGEGLGRVAYADSRQHRDILQSLVRALHTGQKRIWLATPYFLPTWK
CCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHH
VRRALRRAAARGVDVRLLLTGPRTDHPSVRYAGQRYYPRLLRAGVKVFEYQPCFLHLKMV
HHHHHHHHHHCCCEEEEEEECCCCCCCCCEECCCHHHHHHHHCCHHHEECCCHHHHHHHH
LIDDWVSLGSCNFDHWNLRFNLEANLEALDPGLTRDVVASFEKDFALSAETTLADWKARP
HHHHHHHHCCCCCCCEEEEEEEECCCHHCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCH
LWRRVKQRLWGWIDRLVVNLLDQRN
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 L-1-phosphatidyl-glycerol [C]

Specific reaction: 2 L-1-phosphatidyl-glycerol = cardiolipin + glycerol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]