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The map label for this gene is pitA [H]

Identifier: 66047243

GI number: 66047243

Start: 4783510

End: 4784982

Strand: Direct

Name: pitA [H]

Synonym: Psyr_4016

Alternate gene names: 66047243

Gene position: 4783510-4784982 (Clockwise)

Preceding gene: 66047242

Following gene: 66047246

Centisome position: 78.5

GC content: 60.62

Gene sequence:

>1473_bases
ATGATCGATTTATTCAGCGGGCTTGATGCCTGGGTGCTTGTGAGCCTGTTGCTCGCCCTGGCTTTCGTCCTCACCTTCGA
GTTCATCAACGGCTTTCATGACACCGCCAACGCGGTGGCGACAGTCATTTACACCAAAGCAATGCCGCCTCATCTGGCCG
TGTTCTTCTCCGGTGTATTCAACTTTCTCGGCGTGTTGCTGGGCGGCGTGGGCGTTGCCTATGCCATCGTGCATCTGCTG
CCGGTCGAACTGCTCATCAACGTCAACACCGGACACGGCCTGGCCATGGTGTTCTCGCTGCTGGCGGCGGCCATTACCTG
GAACCTCGGGACTTGGTACTTCGGTATTCCGGCCTCCAGTTCGCACACGCTGATCGGCTCGATACTCGGTGTGGGTCTGG
CCAATGCCCTGCTCAACGATATCCCGTTGGCCGATGGCGTGAACTGGCAGAAAGCGATCGATATCGGCGCTTCGCTGGTG
TTCTCGCCGCTGGCCGGCTTTATCGTTGCGGGTCTGGTGCTGGTTGCCCTGAAATGGTGGCGCCCGCTGTCGAAGATGCA
CAAGACCCCTGACCAGCGCCGCAAGCTGGACGACAAGAAGCACCCTCCGTTCTGGAACCGTCTGGTACTGGTCATTTCCG
CCATGGCAGTCAGCTTCGTACACGGTTCGAATGACGGCCAGAAAGGCATTGGCCTGATCATGCTGGTGCTGATCGGCATC
GTGCCCAGCCAGTTCGTGCTTGACCTGACCAGCACGACCTACCAGATCGAACGTACCCGCGATGCGACCCAGCATCTGAG
CCAGTTCTATCAGCGCAACAGCAGCACGCTGGGCGAATATCTGGCCATGGGCAAAGCCGAAAAAGGCGACCTGCCGTCCA
GCTCCGCGTGTAATCCGAAGCAGACCGAACCTACCATCGACGCGCTGCTCGACCGCCTGAAGGGCGTGTCCGACTACCAC
GCGCTGGCCCCTGAAAGCCGCATCGAAGTGCGTCGCTATCTGTTGTGCCTGGACGACACCGCCCGCAAGGTCGGCAAACT
TCCAGACCTGTCAGCGCGTGAAAAATCCGATCTGGAGAAACTTCGCAAGGACCTGACCACCACCACCGAGTACGCACCGT
TCTGGGTGATTCTGGCGGTCGCACTGGCGCTGGGTATCGGCACCATGGTCGGCTGGAAGCGTGTAGTCCTGACGATCGGC
GAGAAGATCGGCAAGCAGGGCATGACTTACGCACAAGGCATGTCCGCGCAAATCACTACCGCCTGCGCAATCGGTCTGGC
CAACGTGTTCAGCCTTCCGGTGTCCACCACGCACATTCTGTCGTCGGGTGTGGCCGGCACCATGGTCGCCAACAAGAGCG
GCCTTCAGGGCGGAACCGTGCGCACCATCCTGCTGGCCTGGGTGCTGACCCTGCCGGCAACGGTCGCCTTGTCCGCAGCG
CTGTTCTGGCTGGCTTCCAAGGCCCTGGCCTGA

Upstream 100 bases:

>100_bases
CTTTTTTTTGGTTTGAGGCGACACCTCGCGGTGACGACAAGTCGTTCGGTATTTTTTCTGTCATAATCGCGCCCCCTTCA
GACCCGGTCAGAAAACCTAC

Downstream 100 bases:

>100_bases
TCTAGGCTCTGTACGAAAAGTCGGCGAGCGAAGGTCAGGCGAGGCAAAAACAGGCGAGGAACGGCCGGGGTCGCGATCGA
CTCTACGTGTTGTAAATGAG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MIDLFSGLDAWVLVSLLLALAFVLTFEFINGFHDTANAVATVIYTKAMPPHLAVFFSGVFNFLGVLLGGVGVAYAIVHLL
PVELLINVNTGHGLAMVFSLLAAAITWNLGTWYFGIPASSSHTLIGSILGVGLANALLNDIPLADGVNWQKAIDIGASLV
FSPLAGFIVAGLVLVALKWWRPLSKMHKTPDQRRKLDDKKHPPFWNRLVLVISAMAVSFVHGSNDGQKGIGLIMLVLIGI
VPSQFVLDLTSTTYQIERTRDATQHLSQFYQRNSSTLGEYLAMGKAEKGDLPSSSACNPKQTEPTIDALLDRLKGVSDYH
ALAPESRIEVRRYLLCLDDTARKVGKLPDLSAREKSDLEKLRKDLTTTTEYAPFWVILAVALALGIGTMVGWKRVVLTIG
EKIGKQGMTYAQGMSAQITTACAIGLANVFSLPVSTTHILSSGVAGTMVANKSGLQGGTVRTILLAWVLTLPATVALSAA
LFWLASKALA

Sequences:

>Translated_490_residues
MIDLFSGLDAWVLVSLLLALAFVLTFEFINGFHDTANAVATVIYTKAMPPHLAVFFSGVFNFLGVLLGGVGVAYAIVHLL
PVELLINVNTGHGLAMVFSLLAAAITWNLGTWYFGIPASSSHTLIGSILGVGLANALLNDIPLADGVNWQKAIDIGASLV
FSPLAGFIVAGLVLVALKWWRPLSKMHKTPDQRRKLDDKKHPPFWNRLVLVISAMAVSFVHGSNDGQKGIGLIMLVLIGI
VPSQFVLDLTSTTYQIERTRDATQHLSQFYQRNSSTLGEYLAMGKAEKGDLPSSSACNPKQTEPTIDALLDRLKGVSDYH
ALAPESRIEVRRYLLCLDDTARKVGKLPDLSAREKSDLEKLRKDLTTTTEYAPFWVILAVALALGIGTMVGWKRVVLTIG
EKIGKQGMTYAQGMSAQITTACAIGLANVFSLPVSTTHILSSGVAGTMVANKSGLQGGTVRTILLAWVLTLPATVALSAA
LFWLASKALA
>Mature_490_residues
MIDLFSGLDAWVLVSLLLALAFVLTFEFINGFHDTANAVATVIYTKAMPPHLAVFFSGVFNFLGVLLGGVGVAYAIVHLL
PVELLINVNTGHGLAMVFSLLAAAITWNLGTWYFGIPASSSHTLIGSILGVGLANALLNDIPLADGVNWQKAIDIGASLV
FSPLAGFIVAGLVLVALKWWRPLSKMHKTPDQRRKLDDKKHPPFWNRLVLVISAMAVSFVHGSNDGQKGIGLIMLVLIGI
VPSQFVLDLTSTTYQIERTRDATQHLSQFYQRNSSTLGEYLAMGKAEKGDLPSSSACNPKQTEPTIDALLDRLKGVSDYH
ALAPESRIEVRRYLLCLDDTARKVGKLPDLSAREKSDLEKLRKDLTTTTEYAPFWVILAVALALGIGTMVGWKRVVLTIG
EKIGKQGMTYAQGMSAQITTACAIGLANVFSLPVSTTHILSSGVAGTMVANKSGLQGGTVRTILLAWVLTLPATVALSAA
LFWLASKALA

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=498, Percent_Identity=55.2208835341365, Blast_Score=520, Evalue=1e-149,
Organism=Escherichia coli, GI1789360, Length=495, Percent_Identity=52.5252525252525, Blast_Score=504, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 52636; Mature: 52636

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]