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The map label for this gene is lepA [H]

Identifier: 66047181

GI number: 66047181

Start: 4700728

End: 4702524

Strand: Reverse

Name: lepA [H]

Synonym: Psyr_3954

Alternate gene names: 66047181

Gene position: 4702524-4700728 (Counterclockwise)

Preceding gene: 66047182

Following gene: 66047180

Centisome position: 77.17

GC content: 58.21

Gene sequence:

>1797_bases
GTGAGTGATTTGAGTCATATCCGCAATTTCTCCATCATCGCCCACATTGACCATGGCAAGTCGACGCTGGCCGACCGCTT
CATTCAAATGTGCGGCGGCCTGACCGAGCGCGAAATGGAAGCGCAGGTGCTTGACTCCATGGATCTTGAGCGCGAGCGCG
GGATCACCATCAAGGCCCATAGCGTTACCCTGTATTACAAGGCCAAAGACGGTATCACCTACCAGCTGAACTTCATTGAC
ACCCCGGGGCACGTCGACTTCACCTACGAAGTCAGCCGTTCTCTGGCCGCGTGCGAAGGCGCGTTGCTGGTGGTCGATGC
AGGGCAGGGCGTCGAGGCGCAATCGGTCGCCAACTGCTACACCGCCATCGAGCAGGGCCTTGAGGTCATGCCGGTACTGA
ACAAGATGGACTTGCCGCAGGCCGATCCGGACCGCGTCAAGGAAGAGATCGAGAAGATCATCGGCATCGACGCCACCGAT
GCCGTGGCGTGCAGCGCCAAGAGCGGCATGGGCGTGGACGAGGTCCTCGAGCGTCTGGTCGCGACCATTCCAGCGCCCAC
CGGCAATATCGAAGATCCGCTGCAGGCGTTGATCATCGACTCCTGGTTCGACAACTACCTGGGCGTCGTGTCTCTGGTGC
GTGTGCGCCACGGCCGCGTCAAGAAGGGCGACAAGATTCTCGTCAAGTCGACCGGCAAGCTGCATCTGGTCGACAGCGTG
GGCGTGTTCAACCCCAAGCACTCGGCGACCGTCGATCTGAAAGCCGGCGAAGTAGGCTTCATCATTGCCGGCATCAAGGA
CATTCACGGTGCGCCGGTCGGTGACACCCTGACCCTGAGCACCACCCCCGACGTCGACGTCCTGCCTGGCTTCAAACGCA
TTCAGCCACAAGTCTATGCAGGCCTGTTTCCGGTCAGCTCGGACGACTTCGAAGACTTCCGTGAAGCGCTGCAAAAGCTG
ACCCTGAACGACTCTTCACTGCAATACCTGCCCGAGAGTTCCGACGCGCTGGGCTTCGGCTTCCGCTGCGGCTTCCTCGG
CATGCTGCACATGGAGATCATCCAGGAACGCCTGGAGCGTGAGTACAATCTCGACCTGATCACCACCGCGCCAACCGTAA
TCTTCGAGCTGTTGCTCAAGACCGGCGAAACGATTTACGTCGATAACCCTTCCAAACTGCCTGACCTGTCGGCCATCGAA
GACATGCGCGAGCCCATCGTTCGCGCAAATATTCTTGTGCCGCAGGAGCACCTGGGCAACGTCATTACGTTGTGCATCGA
AAAACGTGGCGTACAGCACGACATGCTGTTCCTCGGCACGCAGGTTCAGGTGAGCTACGATTTGCCGATGAACGAAGTCG
TTCTGGACTTCTTCGATCGTCTCAAGTCTGTCAGCCGCGGTTATGCTTCGCTGGATTATCATTTTGATCGCTACCAGTCG
GCCAACCTGGTCAAGCTCGACGTGCTGATCAACGCCGAGAAGGTCGACGCCCTGGCCCTGATCGTTCACCGCGACAACGC
ACACTACAAGGGGCGTGCGTTGACCGAGAAGATGAAAGAGCTGATCCCTCGACAGATGTTCGACGTGGCAATTCAGGCGG
CTATCGGCGGTCAGATTGTGGCCCGGACAACCGTCAAGGCGCTTAGAAAGAACGTATTGGCCAAATGCTACGGCGGTGAC
GTCAGCCGCAAGCGTAAATTGCTGGAGAAGCAAAAGGCCGGTAAGAAACGCATGAAGCAGGTCGGCAACGTGGAAATTCC
ACAGGAAGCCTTCCTTGCAGTGCTCAGGTTGGAATAG

Upstream 100 bases:

>100_bases
CCGGGTGCCTGTAAGCGTGACGCCTCTTGCTTAGTTCAGGTACAATTCCCGGCTATTTTTCGGCGGGCAATCTGCCTGCA
ACCTTTTTGAGTGTTGATCC

Downstream 100 bases:

>100_bases
TCAGGTCCTATGTCACTAAATTTCCCGCTGTTGTTGGTCATCGCTGTCTTCGTCTGTGGCGTGCTTGCACTGATTGATCT
GGTCATCCTGGCGCCTCGCC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MSDLSHIRNFSIIAHIDHGKSTLADRFIQMCGGLTEREMEAQVLDSMDLERERGITIKAHSVTLYYKAKDGITYQLNFID
TPGHVDFTYEVSRSLAACEGALLVVDAGQGVEAQSVANCYTAIEQGLEVMPVLNKMDLPQADPDRVKEEIEKIIGIDATD
AVACSAKSGMGVDEVLERLVATIPAPTGNIEDPLQALIIDSWFDNYLGVVSLVRVRHGRVKKGDKILVKSTGKLHLVDSV
GVFNPKHSATVDLKAGEVGFIIAGIKDIHGAPVGDTLTLSTTPDVDVLPGFKRIQPQVYAGLFPVSSDDFEDFREALQKL
TLNDSSLQYLPESSDALGFGFRCGFLGMLHMEIIQERLEREYNLDLITTAPTVIFELLLKTGETIYVDNPSKLPDLSAIE
DMREPIVRANILVPQEHLGNVITLCIEKRGVQHDMLFLGTQVQVSYDLPMNEVVLDFFDRLKSVSRGYASLDYHFDRYQS
ANLVKLDVLINAEKVDALALIVHRDNAHYKGRALTEKMKELIPRQMFDVAIQAAIGGQIVARTTVKALRKNVLAKCYGGD
VSRKRKLLEKQKAGKKRMKQVGNVEIPQEAFLAVLRLE

Sequences:

>Translated_598_residues
MSDLSHIRNFSIIAHIDHGKSTLADRFIQMCGGLTEREMEAQVLDSMDLERERGITIKAHSVTLYYKAKDGITYQLNFID
TPGHVDFTYEVSRSLAACEGALLVVDAGQGVEAQSVANCYTAIEQGLEVMPVLNKMDLPQADPDRVKEEIEKIIGIDATD
AVACSAKSGMGVDEVLERLVATIPAPTGNIEDPLQALIIDSWFDNYLGVVSLVRVRHGRVKKGDKILVKSTGKLHLVDSV
GVFNPKHSATVDLKAGEVGFIIAGIKDIHGAPVGDTLTLSTTPDVDVLPGFKRIQPQVYAGLFPVSSDDFEDFREALQKL
TLNDSSLQYLPESSDALGFGFRCGFLGMLHMEIIQERLEREYNLDLITTAPTVIFELLLKTGETIYVDNPSKLPDLSAIE
DMREPIVRANILVPQEHLGNVITLCIEKRGVQHDMLFLGTQVQVSYDLPMNEVVLDFFDRLKSVSRGYASLDYHFDRYQS
ANLVKLDVLINAEKVDALALIVHRDNAHYKGRALTEKMKELIPRQMFDVAIQAAIGGQIVARTTVKALRKNVLAKCYGGD
VSRKRKLLEKQKAGKKRMKQVGNVEIPQEAFLAVLRLE
>Mature_597_residues
SDLSHIRNFSIIAHIDHGKSTLADRFIQMCGGLTEREMEAQVLDSMDLERERGITIKAHSVTLYYKAKDGITYQLNFIDT
PGHVDFTYEVSRSLAACEGALLVVDAGQGVEAQSVANCYTAIEQGLEVMPVLNKMDLPQADPDRVKEEIEKIIGIDATDA
VACSAKSGMGVDEVLERLVATIPAPTGNIEDPLQALIIDSWFDNYLGVVSLVRVRHGRVKKGDKILVKSTGKLHLVDSVG
VFNPKHSATVDLKAGEVGFIIAGIKDIHGAPVGDTLTLSTTPDVDVLPGFKRIQPQVYAGLFPVSSDDFEDFREALQKLT
LNDSSLQYLPESSDALGFGFRCGFLGMLHMEIIQERLEREYNLDLITTAPTVIFELLLKTGETIYVDNPSKLPDLSAIED
MREPIVRANILVPQEHLGNVITLCIEKRGVQHDMLFLGTQVQVSYDLPMNEVVLDFFDRLKSVSRGYASLDYHFDRYQSA
NLVKLDVLINAEKVDALALIVHRDNAHYKGRALTEKMKELIPRQMFDVAIQAAIGGQIVARTTVKALRKNVLAKCYGGDV
SRKRKLLEKQKAGKKRMKQVGNVEIPQEAFLAVLRLE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=604, Percent_Identity=49.1721854304636, Blast_Score=626, Evalue=1e-179,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=39.5973154362416, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI94966754, Length=166, Percent_Identity=36.7469879518072, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI25306283, Length=137, Percent_Identity=43.7956204379562, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI19923640, Length=137, Percent_Identity=43.7956204379562, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI25306287, Length=137, Percent_Identity=43.7956204379562, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI18390331, Length=158, Percent_Identity=37.3417721518987, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=39.8230088495575, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=39.8230088495575, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=39.8230088495575, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI217272894, Length=173, Percent_Identity=29.4797687861272, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI217272892, Length=173, Percent_Identity=29.4797687861272, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI53729339, Length=276, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI53729337, Length=276, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI4503471, Length=336, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=72.5589225589226, Blast_Score=886, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=505, Percent_Identity=28.5148514851485, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI1789738, Length=161, Percent_Identity=36.0248447204969, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1789559, Length=227, Percent_Identity=32.15859030837, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1790835, Length=163, Percent_Identity=29.4478527607362, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=42.4590163934426, Blast_Score=505, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17556745, Length=161, Percent_Identity=33.5403726708075, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=34.7826086956522, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=36.3013698630137, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=35.3383458646617, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=35.3383458646617, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=152, Percent_Identity=32.8947368421053, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25141371, Length=240, Percent_Identity=30, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=45.0166112956811, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=38.9261744966443, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=38.9261744966443, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=160, Percent_Identity=38.75, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=117, Percent_Identity=39.3162393162393, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=149, Percent_Identity=36.9127516778523, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6325337, Length=291, Percent_Identity=25.4295532646048, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319594, Length=291, Percent_Identity=25.4295532646048, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI78706572, Length=599, Percent_Identity=44.0734557595993, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI28574573, Length=175, Percent_Identity=38.8571428571429, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=35.4037267080745, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585709, Length=174, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585711, Length=174, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585713, Length=174, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI221458488, Length=141, Percent_Identity=42.5531914893617, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=163, Percent_Identity=31.9018404907975, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45553807, Length=320, Percent_Identity=27.5, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45553816, Length=320, Percent_Identity=27.5, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24651721, Length=320, Percent_Identity=27.5, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17864154, Length=320, Percent_Identity=27.5, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652838, Length=204, Percent_Identity=32.3529411764706, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137572, Length=204, Percent_Identity=32.3529411764706, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28572034, Length=231, Percent_Identity=27.7056277056277, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66246; Mature: 66115

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLSHIRNFSIIAHIDHGKSTLADRFIQMCGGLTEREMEAQVLDSMDLERERGITIKAH
CCCHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEE
SVTLYYKAKDGITYQLNFIDTPGHVDFTYEVSRSLAACEGALLVVDAGQGVEAQSVANCY
EEEEEEEECCCEEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
TAIEQGLEVMPVLNKMDLPQADPDRVKEEIEKIIGIDATDAVACSAKSGMGVDEVLERLV
HHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHEECCCCCCCHHHHHHHHH
ATIPAPTGNIEDPLQALIIDSWFDNYLGVVSLVRVRHGRVKKGDKILVKSTGKLHLVDSV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECC
GVFNPKHSATVDLKAGEVGFIIAGIKDIHGAPVGDTLTLSTTPDVDVLPGFKRIQPQVYA
CCCCCCCCCEEEEECCCEEEEEECHHHHCCCCCCCEEEECCCCCCCCCCCHHHHCCHHHH
GLFPVSSDDFEDFREALQKLTLNDSSLQYLPESSDALGFGFRCGFLGMLHMEIIQERLER
EECCCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EYNLDLITTAPTVIFELLLKTGETIYVDNPSKLPDLSAIEDMREPIVRANILVPQEHLGN
HCCCEEEECCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHEEECCHHHHCH
VITLCIEKRGVQHDMLFLGTQVQVSYDLPMNEVVLDFFDRLKSVSRGYASLDYHFDRYQS
HEEHHHHHCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCHHHHCCC
ANLVKLDVLINAEKVDALALIVHRDNAHYKGRALTEKMKELIPRQMFDVAIQAAIGGQIV
CCEEEEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARTTVKALRKNVLAKCYGGDVSRKRKLLEKQKAGKKRMKQVGNVEIPQEAFLAVLRLE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SDLSHIRNFSIIAHIDHGKSTLADRFIQMCGGLTEREMEAQVLDSMDLERERGITIKAH
CCHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEE
SVTLYYKAKDGITYQLNFIDTPGHVDFTYEVSRSLAACEGALLVVDAGQGVEAQSVANCY
EEEEEEEECCCEEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
TAIEQGLEVMPVLNKMDLPQADPDRVKEEIEKIIGIDATDAVACSAKSGMGVDEVLERLV
HHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHEECCCCCCCHHHHHHHHH
ATIPAPTGNIEDPLQALIIDSWFDNYLGVVSLVRVRHGRVKKGDKILVKSTGKLHLVDSV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECC
GVFNPKHSATVDLKAGEVGFIIAGIKDIHGAPVGDTLTLSTTPDVDVLPGFKRIQPQVYA
CCCCCCCCCEEEEECCCEEEEEECHHHHCCCCCCCEEEECCCCCCCCCCCHHHHCCHHHH
GLFPVSSDDFEDFREALQKLTLNDSSLQYLPESSDALGFGFRCGFLGMLHMEIIQERLER
EECCCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EYNLDLITTAPTVIFELLLKTGETIYVDNPSKLPDLSAIEDMREPIVRANILVPQEHLGN
HCCCEEEECCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHEEECCHHHHCH
VITLCIEKRGVQHDMLFLGTQVQVSYDLPMNEVVLDFFDRLKSVSRGYASLDYHFDRYQS
HEEHHHHHCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCHHHHCCC
ANLVKLDVLINAEKVDALALIVHRDNAHYKGRALTEKMKELIPRQMFDVAIQAAIGGQIV
CCEEEEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARTTVKALRKNVLAKCYGGDVSRKRKLLEKQKAGKKRMKQVGNVEIPQEAFLAVLRLE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA