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The map label for this gene is gatA [H]

Identifier: 66047165

GI number: 66047165

Start: 4683313

End: 4684686

Strand: Reverse

Name: gatA [H]

Synonym: Psyr_3938

Alternate gene names: 66047165

Gene position: 4684686-4683313 (Counterclockwise)

Preceding gene: 66047167

Following gene: 66047164

Centisome position: 76.88

GC content: 65.79

Gene sequence:

>1374_bases
ATGACCGATACCGGGAGTCACAGCGTTACCCGTGATGCCTGCGCACTGGCCGAGGATTTTGCTCAGGGGCGCAGTGACCC
TGTGCAGGCTCTGGACAAGGCCCTGCAACAGGCGGCACGCGCCGATCATGTGTTCATTTCCATGAGTATCGAACGGGCAC
GCCGGGAAGCCGAAGCGTCTGCGGCCCGCTGGCAGCAAGGGCAGCCATTGAGCCCCTTCGACGGCGTGCCCATCGCCTGG
AAGGATCTTTTCGACGTGGCAGGCAGCGTCACGACCGCCGGCGCCGCGGTGCGCAACAACCTGTCGCCCGCGCTGCTGGA
TGCGCCGAGTGTCGGGCTGCTGGCAAGGTCCGGGATGGTCAGCCTGGGCAAGACCAACCTCAGTGAGTTCGCTTATTCAG
GGCTGGGCCTGAACCCGCATTTCGGCACGCCGATCAACCCCAACAGCGACAGCTCGCCGCGCATTCCGGGCGGTTCGTCT
TCAGGTTCGGCAGTCGCCGTGGCCAACGGAACCGTACCGATTGCAATGGGCACCGATACCGCCGGTTCTATTCGTGTGCC
GGCAGCGCTCAATGGCCTGGTGGGTTTTCGCAGTACCAGCCGCCGCTACAGCCGCGACGGCGTTTTCCCGCTGGCGCTGA
CGCTGGACAGCGTCGGGCCACTGACCCGCAGCGTGCGCGACGCTCTGGTGATCGACGATCTGCTCTGTGCGCGCAGCAAA
CCTTCATCGTTGATACCACGCAGCCTGGCCGGTCAGCGTTTTCTGCTCGATCAGGCGGTGCTGGAAGACGAGCGCGTCAC
GCCGGCCGTTCGCGACAACCTGTTGCGTGCCGTCGAAGCCTTGCGTGCAAGTGGCGCGCTGATCGAGGTCAAGCCTTGCC
AGGCATTTCAGGCCACCTTGCTGTTGATCCAGCAACAGGGCTGGCTTGGCGCAGCCGAAGCGTTCGCGCTGCATCAGGCG
TTGCTCGACAGCGACGCCGCCAGCCAGCTCGACCCCAGGGTTCGCAAGCGCCTGGAGGCCGCCCGTTCCATGCCGGCCAG
CCTGCTGGTCAATCTGTATGCCGCGCGCGAGCGGTTGCAGGAGCAGTTGACCCTCGAACTGGACGGCGCGCTGCTGATTA
CCCCGACCGTGGCCCATGTGGCGCCGCCGCTCGTACCGCTGCTCAAGGACGACGATCTGTTCGTTCAAACCAACCTCGCC
ACGCTGCGCCTGACCATGCCCGGCAGCCTGCTGAACATGCCCGGCGTGTCATTGCCCAGCGGCCGCGATGCCTCGGGCCT
GCCCACCGGGCTGTTGCTCAGCGCACCGGCAGGCGAAGACGCACGGCTGCTGCGTGCGGCGCTGACTGTGGAGTCGTTGA
TAGCAGCCCCTTGA

Upstream 100 bases:

>100_bases
TCGATCGGGTCAGGGCGTCGCGACGTGCAGATTGGCATGGAACCTGCCTTTGTTAAATGTACATTTTATTAATAAGTACA
TTTTTAAAGGTGTGATGTCC

Downstream 100 bases:

>100_bases
ACACGTTTTGCAGTGCCAACCGGCCCGCGCGCCGTACCGCGCGAAGCCGATCTGATTAACCCGTGGGAGAACAACGATGG
CCAAAGAAATTCTGTGTGCG

Product: hypothetical protein

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTDTGSHSVTRDACALAEDFAQGRSDPVQALDKALQQAARADHVFISMSIERARREAEASAARWQQGQPLSPFDGVPIAW
KDLFDVAGSVTTAGAAVRNNLSPALLDAPSVGLLARSGMVSLGKTNLSEFAYSGLGLNPHFGTPINPNSDSSPRIPGGSS
SGSAVAVANGTVPIAMGTDTAGSIRVPAALNGLVGFRSTSRRYSRDGVFPLALTLDSVGPLTRSVRDALVIDDLLCARSK
PSSLIPRSLAGQRFLLDQAVLEDERVTPAVRDNLLRAVEALRASGALIEVKPCQAFQATLLLIQQQGWLGAAEAFALHQA
LLDSDAASQLDPRVRKRLEAARSMPASLLVNLYAARERLQEQLTLELDGALLITPTVAHVAPPLVPLLKDDDLFVQTNLA
TLRLTMPGSLLNMPGVSLPSGRDASGLPTGLLLSAPAGEDARLLRAALTVESLIAAP

Sequences:

>Translated_457_residues
MTDTGSHSVTRDACALAEDFAQGRSDPVQALDKALQQAARADHVFISMSIERARREAEASAARWQQGQPLSPFDGVPIAW
KDLFDVAGSVTTAGAAVRNNLSPALLDAPSVGLLARSGMVSLGKTNLSEFAYSGLGLNPHFGTPINPNSDSSPRIPGGSS
SGSAVAVANGTVPIAMGTDTAGSIRVPAALNGLVGFRSTSRRYSRDGVFPLALTLDSVGPLTRSVRDALVIDDLLCARSK
PSSLIPRSLAGQRFLLDQAVLEDERVTPAVRDNLLRAVEALRASGALIEVKPCQAFQATLLLIQQQGWLGAAEAFALHQA
LLDSDAASQLDPRVRKRLEAARSMPASLLVNLYAARERLQEQLTLELDGALLITPTVAHVAPPLVPLLKDDDLFVQTNLA
TLRLTMPGSLLNMPGVSLPSGRDASGLPTGLLLSAPAGEDARLLRAALTVESLIAAP
>Mature_456_residues
TDTGSHSVTRDACALAEDFAQGRSDPVQALDKALQQAARADHVFISMSIERARREAEASAARWQQGQPLSPFDGVPIAWK
DLFDVAGSVTTAGAAVRNNLSPALLDAPSVGLLARSGMVSLGKTNLSEFAYSGLGLNPHFGTPINPNSDSSPRIPGGSSS
GSAVAVANGTVPIAMGTDTAGSIRVPAALNGLVGFRSTSRRYSRDGVFPLALTLDSVGPLTRSVRDALVIDDLLCARSKP
SSLIPRSLAGQRFLLDQAVLEDERVTPAVRDNLLRAVEALRASGALIEVKPCQAFQATLLLIQQQGWLGAAEAFALHQAL
LDSDAASQLDPRVRKRLEAARSMPASLLVNLYAARERLQEQLTLELDGALLITPTVAHVAPPLVPLLKDDDLFVQTNLAT
LRLTMPGSLLNMPGVSLPSGRDASGLPTGLLLSAPAGEDARLLRAALTVESLIAAP

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=230, Percent_Identity=37.3913043478261, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI195972892, Length=213, Percent_Identity=30.5164319248826, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=459, Percent_Identity=27.4509803921569, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=194, Percent_Identity=35.0515463917526, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=202, Percent_Identity=29.7029702970297, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=214, Percent_Identity=31.7757009345794, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=264, Percent_Identity=29.5454545454545, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556276, Length=264, Percent_Identity=29.5454545454545, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=436, Percent_Identity=30.5045871559633, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323950, Length=136, Percent_Identity=41.9117647058824, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648113, Length=222, Percent_Identity=35.5855855855856, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24652985, Length=478, Percent_Identity=24.8953974895398, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922090, Length=478, Percent_Identity=24.8953974895398, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652981, Length=478, Percent_Identity=24.8953974895398, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652983, Length=478, Percent_Identity=24.8953974895398, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356731, Length=239, Percent_Identity=29.2887029288703, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI45550774, Length=235, Percent_Identity=28.0851063829787, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648435, Length=235, Percent_Identity=28.0851063829787, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648437, Length=235, Percent_Identity=28.0851063829787, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24644968, Length=216, Percent_Identity=29.6296296296296, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 47967; Mature: 47836

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTGSHSVTRDACALAEDFAQGRSDPVQALDKALQQAARADHVFISMSIERARREAEAS
CCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHH
AARWQQGQPLSPFDGVPIAWKDLFDVAGSVTTAGAAVRNNLSPALLDAPSVGLLARSGMV
HHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCHHCCCCCCHHHHHCCCC
SLGKTNLSEFAYSGLGLNPHFGTPINPNSDSSPRIPGGSSSGSAVAVANGTVPIAMGTDT
CCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCC
AGSIRVPAALNGLVGFRSTSRRYSRDGVFPLALTLDSVGPLTRSVRDALVIDDLLCARSK
CCCEECCHHHHHHHHCCHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
PSSLIPRSLAGQRFLLDQAVLEDERVTPAVRDNLLRAVEALRASGALIEVKPCQAFQATL
CCHHCCHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHH
LLIQQQGWLGAAEAFALHQALLDSDAASQLDPRVRKRLEAARSMPASLLVNLYAARERLQ
HHHHCCCCCCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
EQLTLELDGALLITPTVAHVAPPLVPLLKDDDLFVQTNLATLRLTMPGSLLNMPGVSLPS
HHHEEEECCEEEECCCHHHHCCCCCHHCCCCCEEEEECCEEEEEECCCHHHCCCCCCCCC
GRDASGLPTGLLLSAPAGEDARLLRAALTVESLIAAP
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDTGSHSVTRDACALAEDFAQGRSDPVQALDKALQQAARADHVFISMSIERARREAEAS
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHH
AARWQQGQPLSPFDGVPIAWKDLFDVAGSVTTAGAAVRNNLSPALLDAPSVGLLARSGMV
HHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCHHCCCCCCHHHHHCCCC
SLGKTNLSEFAYSGLGLNPHFGTPINPNSDSSPRIPGGSSSGSAVAVANGTVPIAMGTDT
CCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCC
AGSIRVPAALNGLVGFRSTSRRYSRDGVFPLALTLDSVGPLTRSVRDALVIDDLLCARSK
CCCEECCHHHHHHHHCCHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
PSSLIPRSLAGQRFLLDQAVLEDERVTPAVRDNLLRAVEALRASGALIEVKPCQAFQATL
CCHHCCHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHH
LLIQQQGWLGAAEAFALHQALLDSDAASQLDPRVRKRLEAARSMPASLLVNLYAARERLQ
HHHHCCCCCCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
EQLTLELDGALLITPTVAHVAPPLVPLLKDDDLFVQTNLATLRLTMPGSLLNMPGVSLPS
HHHEEEECCEEEECCCHHHHCCCCCHHCCCCCEEEEECCEEEEEECCCHHHCCCCCCCCC
GRDASGLPTGLLLSAPAGEDARLLRAALTVESLIAAP
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA