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The map label for this gene is metE [H]

Identifier: 66047143

GI number: 66047143

Start: 4659698

End: 4660495

Strand: Reverse

Name: metE [H]

Synonym: Psyr_3916

Alternate gene names: 66047143

Gene position: 4660495-4659698 (Counterclockwise)

Preceding gene: 66047145

Following gene: 66047142

Centisome position: 76.48

GC content: 59.15

Gene sequence:

>798_bases
ATGAGTTTGTCTCATGTTGTGTCAGCTGTCGACAATAGCCCCATCTTTTATGGAGGACGCATCGTGGCCCTGACTCAACA
CCCGGACTATTTTCACATTGGCGCAGACCGTGAACTGCAGCACGCTCTGCAGGCTTTCTGGAAACAGGATCAGGGTGTTA
CGACCACGGGCGCCGAATTGCGCACCTGGTTGATGGCGCGTTACCCGCAGCAGGTGCCACACCTGAAACGCGACCAGCGT
TTTCAGCCCGATTGGGCGTCGTTGCTGGCGAGCGTCGCTGAAGCAGGCGAGCAAGGCCGCAAACCTGCATTGATCGGTCC
TCTGACACTGCTCTGGCTAAGTGACGTTCAGGGTGACGAGGTAGATCGCCTCGACCTGCTGGAGCATCTGCTGCCGGTGT
ATGGCGAGATCTTCGGGCGTCTGGCTGCGCGCGGTGTCGAGTGGATTCAGATCGACGAACCGATCCTCGCGCTCGACCTG
CCGCTGGCATGGAGCAACGCCTTCGAACGTGCCTATCACATTCTTCAGTATTCGCCGTTGAAGAAGCTCATCGGCCTCTA
TCACGGTGACCTGCGGCCCAACCTGGGTGTCGCGGCCTTGCTGCCGGTTGCAGGGTTGCATCTGGACAGCGTGACCGAAC
CGGAGCTGCTGGCCCCGGTGTTTGATCGTCTGCCCGTTTACAAGGTTCTGTCTCTGGGCGAGTGCGATACGCATTCGAAC
TGGCATCAGGAATCGTTGCTCGAAGCGCGCGCCCGTTTTGGGGAAAACCTGATGGTGGCGGATCAGGTCGCTGCGTAA

Upstream 100 bases:

>100_bases
AAGGCTGTCGGCCTCGCGCAGGGCATGCAGGGTTTTGAGGTGGCGTATTTCAAGCACGGTCAGTGCTCCATGAGCAGTAT
TTGTGATCAACACGAAAAAA

Downstream 100 bases:

>100_bases
TGTAATACCCGATTAACGACCGCTCAGCGCCATTCATCAAACTGTCACGCAAGTGTGGCAGCGCGCTTGGAAAAACATCA
TCAGACTCGCGGCCTACTGG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSLSHVVSAVDNSPIFYGGRIVALTQHPDYFHIGADRELQHALQAFWKQDQGVTTTGAELRTWLMARYPQQVPHLKRDQR
FQPDWASLLASVAEAGEQGRKPALIGPLTLLWLSDVQGDEVDRLDLLEHLLPVYGEIFGRLAARGVEWIQIDEPILALDL
PLAWSNAFERAYHILQYSPLKKLIGLYHGDLRPNLGVAALLPVAGLHLDSVTEPELLAPVFDRLPVYKVLSLGECDTHSN
WHQESLLEARARFGENLMVADQVAA

Sequences:

>Translated_265_residues
MSLSHVVSAVDNSPIFYGGRIVALTQHPDYFHIGADRELQHALQAFWKQDQGVTTTGAELRTWLMARYPQQVPHLKRDQR
FQPDWASLLASVAEAGEQGRKPALIGPLTLLWLSDVQGDEVDRLDLLEHLLPVYGEIFGRLAARGVEWIQIDEPILALDL
PLAWSNAFERAYHILQYSPLKKLIGLYHGDLRPNLGVAALLPVAGLHLDSVTEPELLAPVFDRLPVYKVLSLGECDTHSN
WHQESLLEARARFGENLMVADQVAA
>Mature_264_residues
SLSHVVSAVDNSPIFYGGRIVALTQHPDYFHIGADRELQHALQAFWKQDQGVTTTGAELRTWLMARYPQQVPHLKRDQRF
QPDWASLLASVAEAGEQGRKPALIGPLTLLWLSDVQGDEVDRLDLLEHLLPVYGEIFGRLAARGVEWIQIDEPILALDLP
LAWSNAFERAYHILQYSPLKKLIGLYHGDLRPNLGVAALLPVAGLHLDSVTEPELLAPVFDRLPVYKVLSLGECDTHSNW
HQESLLEARARFGENLMVADQVAA

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=195, Percent_Identity=41.025641025641, Blast_Score=141, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320936, Length=201, Percent_Identity=31.3432835820896, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 29645; Mature: 29514

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSHVVSAVDNSPIFYGGRIVALTQHPDYFHIGADRELQHALQAFWKQDQGVTTTGAEL
CCHHHHHHHHCCCCEEECCEEEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCCCCHHHH
RTWLMARYPQQVPHLKRDQRFQPDWASLLASVAEAGEQGRKPALIGPLTLLWLSDVQGDE
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCC
VDRLDLLEHLLPVYGEIFGRLAARGVEWIQIDEPILALDLPLAWSNAFERAYHILQYSPL
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHH
KKLIGLYHGDLRPNLGVAALLPVAGLHLDSVTEPELLAPVFDRLPVYKVLSLGECDTHSN
HHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCC
WHQESLLEARARFGENLMVADQVAA
HHHHHHHHHHHHHCCCCEEHHHHCC
>Mature Secondary Structure 
SLSHVVSAVDNSPIFYGGRIVALTQHPDYFHIGADRELQHALQAFWKQDQGVTTTGAEL
CHHHHHHHHCCCCEEECCEEEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCCCCHHHH
RTWLMARYPQQVPHLKRDQRFQPDWASLLASVAEAGEQGRKPALIGPLTLLWLSDVQGDE
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCC
VDRLDLLEHLLPVYGEIFGRLAARGVEWIQIDEPILALDLPLAWSNAFERAYHILQYSPL
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHH
KKLIGLYHGDLRPNLGVAALLPVAGLHLDSVTEPELLAPVFDRLPVYKVLSLGECDTHSN
HHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCC
WHQESLLEARARFGENLMVADQVAA
HHHHHHHHHHHHHCCCCEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]