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The map label for this gene is glpF [H]

Identifier: 66047131

GI number: 66047131

Start: 4645020

End: 4645877

Strand: Direct

Name: glpF [H]

Synonym: Psyr_3904

Alternate gene names: 66047131

Gene position: 4645020-4645877 (Clockwise)

Preceding gene: 66047129

Following gene: 66047132

Centisome position: 76.23

GC content: 57.81

Gene sequence:

>858_bases
ATGTCCATCGCACTAAAACAACCGACGCTCACCGGGCAATGTGTGGCTGAATTTCTTGGCACCGCCCTGATGATTTTTTT
CGGCACAGGATGCGTTGCAGCGCTCAAGGTAGCGGGAGCCACCTTCGGCCTTTGGGAAATCAGCATCATCTGGGGCATTG
CCGTCAGCATGGGGATCTACCTCAGCGCCGGCATTTCGGGTGCCCACCTCAACCCGGCCGTCAGCATCGCGCTGAGTCTG
TTCGCCGGTTTTGAAAAACGTAAATTGCCCTTCTATATCTCGGCGCAAATCGCAGGCGCGTTCTGCGGAGCGGGTCTGGT
GTACCTGCTGTACATCAGCCTTTTTTTCGACTTCGAACATGCTCACCACATCATTCGCGGCAGCGAGCAGAGTCTTGAAC
TGGCCTCGGTGTTCTCGACGTATCCGAACCCGGCCATTTCGGTCGGTCAGGCCTTTCTGGTCGAAGTGGTCATCACCACC
ATTCTGATGGGTGTGATCATGGCCTTGGGCGATGACAGCAATGGTTTGCCACGCGGCCCGCTGGCACCCTTGCTGATCGG
CCTGCTGGTAGCCGTGATCGGCAGCTCGATGGGCCCGCTGACCGGTTTCGCCATGAACCCGGCACGCGACTTCGGTCCGA
AGCTGATGACCTTCTTCGCTGGCTGGGGTGAAATGGCCTTCACCGGTGGTCGCGACATTCCTTATTTCCTGGTTCCGATT
TTTGCGCCCATTCTGGGTGCCTGCCTTGGTGCTGCCGGCTACCGCGCGCTGATTGCGCGTCATCTGCCAAGCGCCGCTCC
TGTTGAAAATGAAAAAGAGGCTCCTGTGGTTCGCGGCAAAGTTCAGGCTTCATCTTGA

Upstream 100 bases:

>100_bases
CGCAACGGCGATCAGAAATGGGGTTTTTTCTGAGACGCACGAATAACGAGCACCCACACCTGTCTCAGGGTGCCGAGACA
AAAACAAAAATGAGGTATCC

Downstream 100 bases:

>100_bases
TTTCACTCAATTTGAATCTCACCCAACTGAATCCCACCCAATAATGCAAGGCAATCGACATGACTGACACACAGAACAAG
AACTACATCATTGCTCTTGA

Product: major intrinsic protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Glycerol diffusion facilitator [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSIALKQPTLTGQCVAEFLGTALMIFFGTGCVAALKVAGATFGLWEISIIWGIAVSMGIYLSAGISGAHLNPAVSIALSL
FAGFEKRKLPFYISAQIAGAFCGAGLVYLLYISLFFDFEHAHHIIRGSEQSLELASVFSTYPNPAISVGQAFLVEVVITT
ILMGVIMALGDDSNGLPRGPLAPLLIGLLVAVIGSSMGPLTGFAMNPARDFGPKLMTFFAGWGEMAFTGGRDIPYFLVPI
FAPILGACLGAAGYRALIARHLPSAAPVENEKEAPVVRGKVQASS

Sequences:

>Translated_285_residues
MSIALKQPTLTGQCVAEFLGTALMIFFGTGCVAALKVAGATFGLWEISIIWGIAVSMGIYLSAGISGAHLNPAVSIALSL
FAGFEKRKLPFYISAQIAGAFCGAGLVYLLYISLFFDFEHAHHIIRGSEQSLELASVFSTYPNPAISVGQAFLVEVVITT
ILMGVIMALGDDSNGLPRGPLAPLLIGLLVAVIGSSMGPLTGFAMNPARDFGPKLMTFFAGWGEMAFTGGRDIPYFLVPI
FAPILGACLGAAGYRALIARHLPSAAPVENEKEAPVVRGKVQASS
>Mature_284_residues
SIALKQPTLTGQCVAEFLGTALMIFFGTGCVAALKVAGATFGLWEISIIWGIAVSMGIYLSAGISGAHLNPAVSIALSLF
AGFEKRKLPFYISAQIAGAFCGAGLVYLLYISLFFDFEHAHHIIRGSEQSLELASVFSTYPNPAISVGQAFLVEVVITTI
LMGVIMALGDDSNGLPRGPLAPLLIGLLVAVIGSSMGPLTGFAMNPARDFGPKLMTFFAGWGEMAFTGGRDIPYFLVPIF
APILGACLGAAGYRALIARHLPSAAPVENEKEAPVVRGKVQASS

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=274, Percent_Identity=40.1459854014599, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI22538420, Length=257, Percent_Identity=38.9105058365759, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI4826645, Length=273, Percent_Identity=38.4615384615385, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI4502187, Length=260, Percent_Identity=33.8461538461538, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI310133356, Length=217, Percent_Identity=29.4930875576037, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI310114181, Length=217, Percent_Identity=29.4930875576037, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI45446752, Length=204, Percent_Identity=32.3529411764706, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4502179, Length=204, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4502183, Length=244, Percent_Identity=32.3770491803279, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI6912506, Length=214, Percent_Identity=30.3738317757009, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI86792455, Length=206, Percent_Identity=31.5533980582524, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790362, Length=268, Percent_Identity=70.8955223880597, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71994009, Length=264, Percent_Identity=33.7121212121212, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71992966, Length=261, Percent_Identity=30.2681992337165, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17544068, Length=262, Percent_Identity=33.969465648855, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17533613, Length=262, Percent_Identity=31.6793893129771, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32564052, Length=262, Percent_Identity=31.6793893129771, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17531429, Length=277, Percent_Identity=30.6859205776173, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17531431, Length=277, Percent_Identity=30.6859205776173, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71992961, Length=261, Percent_Identity=27.5862068965517, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71993722, Length=244, Percent_Identity=27.4590163934426, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI212646268, Length=279, Percent_Identity=26.5232974910394, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558372, Length=257, Percent_Identity=26.8482490272374, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=213, Percent_Identity=38.9671361502347, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322985, Length=294, Percent_Identity=29.2517006802721, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24762344, Length=259, Percent_Identity=30.1158301158301, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24762346, Length=259, Percent_Identity=30.1158301158301, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24762348, Length=259, Percent_Identity=30.1158301158301, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20130305, Length=259, Percent_Identity=30.1158301158301, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550503, Length=211, Percent_Identity=28.436018957346, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24652747, Length=202, Percent_Identity=30.6930693069307, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI45551084, Length=202, Percent_Identity=30.6930693069307, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 29846; Mature: 29714

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIALKQPTLTGQCVAEFLGTALMIFFGTGCVAALKVAGATFGLWEISIIWGIAVSMGIY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LSAGISGAHLNPAVSIALSLFAGFEKRKLPFYISAQIAGAFCGAGLVYLLYISLFFDFEH
HHCCCCCCCCCHHHHHHHHHHHCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AHHIIRGSEQSLELASVFSTYPNPAISVGQAFLVEVVITTILMGVIMALGDDSNGLPRGP
HHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LAPLLIGLLVAVIGSSMGPLTGFAMNPARDFGPKLMTFFAGWGEMAFTGGRDIPYFLVPI
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHH
FAPILGACLGAAGYRALIARHLPSAAPVENEKEAPVVRGKVQASS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
SIALKQPTLTGQCVAEFLGTALMIFFGTGCVAALKVAGATFGLWEISIIWGIAVSMGIY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LSAGISGAHLNPAVSIALSLFAGFEKRKLPFYISAQIAGAFCGAGLVYLLYISLFFDFEH
HHCCCCCCCCCHHHHHHHHHHHCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AHHIIRGSEQSLELASVFSTYPNPAISVGQAFLVEVVITTILMGVIMALGDDSNGLPRGP
HHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LAPLLIGLLVAVIGSSMGPLTGFAMNPARDFGPKLMTFFAGWGEMAFTGGRDIPYFLVPI
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHH
FAPILGACLGAAGYRALIARHLPSAAPVENEKEAPVVRGKVQASS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9141691; 10984043 [H]