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The map label for this gene is mscM [H]

Identifier: 66047031

GI number: 66047031

Start: 4520428

End: 4521720

Strand: Direct

Name: mscM [H]

Synonym: Psyr_3803

Alternate gene names: 66047031

Gene position: 4520428-4521720 (Clockwise)

Preceding gene: 66047029

Following gene: 66047032

Centisome position: 74.18

GC content: 61.18

Gene sequence:

>1293_bases
ATGGATATTCACTCGCTTTGGCTCAAGACCCAGGAACTCTGGGACATGCTCGATCAGCACCCGTGGGTACGCACCGGGCT
GGCGCTGATCCTGTTGCTGACCGCCGCACTGGTGCTTGGCCGCGTGGCGCGCTTTCTGGTGCTGTACGCCGTCAGGATGC
TCGGCCGTCAGCCGTCCCTGCATTGGGTCAACGACTTCCGCCACAACAAGGTTTTCCATCGCCTGGCGCAGATGGTGCCG
TCGCTGGTCATCCAGTTCGGCCTCACCCTGGTCCCGGGCCTGAGTACTGCGGGCCGGAACGTGATCGGCAACATCGCCAT
GGCGTTCACCATCCTGTTCATGACCCTGGCCATCGGCACCCTGCTCAACGCCCTGCTGGACATCTACGCGCGCACCGAAC
ACGCCCGCACGCGCTCCATCAAGGGTTACGTGCAGCTGTCGAAGATGATTCTTTACGTCTTCGCCGGGATCATCATCGTC
GCCACCCTGATCGACCGTTCGCCCCTGCTACTGCTGTCCGGCCTCGGCGCCATGTCGGCGGTCATCCTGTTGGTCTACAA
GGACACCCTGCTCTCCTTCGTCGCCAGCGTGCAGCTGACCAGCAACGACATGCTGCGCGTCGGCGACTGGATCGAAATGC
CGCAAGTGGGCGCGGATGGCGATGTGGTGGACATCACCCTGCACACCGTCAAGGTGCAGAACTTCGACAAAACCATCGTC
TCCATCCCGACCTGGCGCCTGATGTCCGAGTCGTTCCGCAACTGGCGCGGCATGCAGCAATCCGGCGGACGGCGCATCAA
GCGCAGCCTGTTCATCGACGCCAGCGGCGTGCGCTTCGTGCGCGACGACGAAGAGCAGCAACTCATGCAAATCCACCTGC
TCACCGACTACATCGGCCGCAAACAGGCCGAACTGCTGGCCTGGAACCAGGCACAAGGCAACGTCGCACAAATGTCCGCC
AACCGCCGGCGCATGACCAACATCGGCACCTTCCGAGCCTACGCCCTGGCATACCTGAAGAGCCACGTCGACATCAACCC
GAACATGACCTGCATGGTTCGCCAGCTCGAACCCACCTCACAGGGCATCCCGCTGGAAATCTACTGCTTCACCCGCACGA
CGGTGTGGGTGGATTACGAGCGGATACAGGGGGATATTTTCGATTATCTGATTACGGTGATGCCGGAGTTCGGGTTGAGT
TTGTATCAGCAGCCTAGTGGGGCGGATATGCGGGTGGGGTTGAGGGGGGAGACTATGAGTCAAGTCACAGCTGACTGGAC
GAAGGAAAGGTGA

Upstream 100 bases:

>100_bases
TCATTGCTCAACGTGCACTTCGTGCTCACAGGCCGCCCCGTCCGACCCGGTAAATGTTCTAATCACGCCCCCCTCTTCGC
TCAACGGGCCCGATGCTTTT

Downstream 100 bases:

>100_bases
GGTTGGGATTTGAAGGTGTCTGAATTGGGGCCATAGCAAATGAAGCAGGAAATTAACTCGGGTGAGAAAATCGCCAGGTA
TCGATATATGTCGCACTTTT

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MDIHSLWLKTQELWDMLDQHPWVRTGLALILLLTAALVLGRVARFLVLYAVRMLGRQPSLHWVNDFRHNKVFHRLAQMVP
SLVIQFGLTLVPGLSTAGRNVIGNIAMAFTILFMTLAIGTLLNALLDIYARTEHARTRSIKGYVQLSKMILYVFAGIIIV
ATLIDRSPLLLLSGLGAMSAVILLVYKDTLLSFVASVQLTSNDMLRVGDWIEMPQVGADGDVVDITLHTVKVQNFDKTIV
SIPTWRLMSESFRNWRGMQQSGGRRIKRSLFIDASGVRFVRDDEEQQLMQIHLLTDYIGRKQAELLAWNQAQGNVAQMSA
NRRRMTNIGTFRAYALAYLKSHVDINPNMTCMVRQLEPTSQGIPLEIYCFTRTTVWVDYERIQGDIFDYLITVMPEFGLS
LYQQPSGADMRVGLRGETMSQVTADWTKER

Sequences:

>Translated_430_residues
MDIHSLWLKTQELWDMLDQHPWVRTGLALILLLTAALVLGRVARFLVLYAVRMLGRQPSLHWVNDFRHNKVFHRLAQMVP
SLVIQFGLTLVPGLSTAGRNVIGNIAMAFTILFMTLAIGTLLNALLDIYARTEHARTRSIKGYVQLSKMILYVFAGIIIV
ATLIDRSPLLLLSGLGAMSAVILLVYKDTLLSFVASVQLTSNDMLRVGDWIEMPQVGADGDVVDITLHTVKVQNFDKTIV
SIPTWRLMSESFRNWRGMQQSGGRRIKRSLFIDASGVRFVRDDEEQQLMQIHLLTDYIGRKQAELLAWNQAQGNVAQMSA
NRRRMTNIGTFRAYALAYLKSHVDINPNMTCMVRQLEPTSQGIPLEIYCFTRTTVWVDYERIQGDIFDYLITVMPEFGLS
LYQQPSGADMRVGLRGETMSQVTADWTKER
>Mature_430_residues
MDIHSLWLKTQELWDMLDQHPWVRTGLALILLLTAALVLGRVARFLVLYAVRMLGRQPSLHWVNDFRHNKVFHRLAQMVP
SLVIQFGLTLVPGLSTAGRNVIGNIAMAFTILFMTLAIGTLLNALLDIYARTEHARTRSIKGYVQLSKMILYVFAGIIIV
ATLIDRSPLLLLSGLGAMSAVILLVYKDTLLSFVASVQLTSNDMLRVGDWIEMPQVGADGDVVDITLHTVKVQNFDKTIV
SIPTWRLMSESFRNWRGMQQSGGRRIKRSLFIDASGVRFVRDDEEQQLMQIHLLTDYIGRKQAELLAWNQAQGNVAQMSA
NRRRMTNIGTFRAYALAYLKSHVDINPNMTCMVRQLEPTSQGIPLEIYCFTRTTVWVDYERIQGDIFDYLITVMPEFGLS
LYQQPSGADMRVGLRGETMSQVTADWTKER

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=382, Percent_Identity=44.5026178010471, Blast_Score=342, Evalue=4e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 48783; Mature: 48783

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIHSLWLKTQELWDMLDQHPWVRTGLALILLLTAALVLGRVARFLVLYAVRMLGRQPSL
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
HWVNDFRHNKVFHRLAQMVPSLVIQFGLTLVPGLSTAGRNVIGNIAMAFTILFMTLAIGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLNALLDIYARTEHARTRSIKGYVQLSKMILYVFAGIIIVATLIDRSPLLLLSGLGAMSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VILLVYKDTLLSFVASVQLTSNDMLRVGDWIEMPQVGADGDVVDITLHTVKVQNFDKTIV
HHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCEEEEEEEEEEECCCCCHHH
SIPTWRLMSESFRNWRGMQQSGGRRIKRSLFIDASGVRFVRDDEEQQLMQIHLLTDYIGR
CCCHHHHHHHHHHHHCCCHHCCCHHHHHHHEEECCCCEEECCCHHHHHHHHHHHHHHHCC
KQAELLAWNQAQGNVAQMSANRRRMTNIGTFRAYALAYLKSHVDINPNMTCMVRQLEPTS
CHHHHHEECCCCCCHHHHCCCHHHHHHCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
QGIPLEIYCFTRTTVWVDYERIQGDIFDYLITVMPEFGLSLYQQPSGADMRVGLRGETMS
CCCCEEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCEEEECCCCHHH
QVTADWTKER
HHHHHHCCCC
>Mature Secondary Structure
MDIHSLWLKTQELWDMLDQHPWVRTGLALILLLTAALVLGRVARFLVLYAVRMLGRQPSL
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
HWVNDFRHNKVFHRLAQMVPSLVIQFGLTLVPGLSTAGRNVIGNIAMAFTILFMTLAIGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLNALLDIYARTEHARTRSIKGYVQLSKMILYVFAGIIIVATLIDRSPLLLLSGLGAMSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VILLVYKDTLLSFVASVQLTSNDMLRVGDWIEMPQVGADGDVVDITLHTVKVQNFDKTIV
HHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCEEEEEEEEEEECCCCCHHH
SIPTWRLMSESFRNWRGMQQSGGRRIKRSLFIDASGVRFVRDDEEQQLMQIHLLTDYIGR
CCCHHHHHHHHHHHHCCCHHCCCHHHHHHHEEECCCCEEECCCHHHHHHHHHHHHHHHCC
KQAELLAWNQAQGNVAQMSANRRRMTNIGTFRAYALAYLKSHVDINPNMTCMVRQLEPTS
CHHHHHEECCCCCCHHHHCCCHHHHHHCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
QGIPLEIYCFTRTTVWVDYERIQGDIFDYLITVMPEFGLSLYQQPSGADMRVGLRGETMS
CCCCEEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCEEEECCCCHHH
QVTADWTKER
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]