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The map label for this gene is mnmC

Identifier: 66046970

GI number: 66046970

Start: 4457020

End: 4459017

Strand: Direct

Name: mnmC

Synonym: Psyr_3742

Alternate gene names: 66046970

Gene position: 4457020-4459017 (Clockwise)

Preceding gene: 66046968

Following gene: 66046971

Centisome position: 73.14

GC content: 63.81

Gene sequence:

>1998_bases
ATGACCATTACCCGCCACGCCCAGATCGACTGGGACGAACACGGCAACCCGCGTTCCCGCGACTTCTCCGACGTGTACTT
TTCCACCGAATCCGGCCTGGAGGAAACGCGCCATGTGTTTCTGGTGCAGAACGACCTGCGCCGGCGCTTCACCGAGTTGC
AGGACGGCGGAAGGCTGATCATTGGCGAGACCGGATTTGGCACCGGGCTGAATTTTCTCTGCGCCTGGCAGTTGTTCGAC
GAGTGCGCGCCCGCCAATGCACGCCTGCAGTTCGTCAGCGTCGAGAAGTACCCGTTGAGCCACGCCGACCTGCAGCGTGC
CCTGGCGTTGTGGCCCGAGCTGTCGCCGTTCGCGGGTCAATTGCTCGACCAGTATGTGGCTGTGCACGAAGGCTTTCAGC
GTCTGGTGTTCGCCGGCGGCCGCGTGACGCTTACATTGCTGATCGGCGATGCACTGCAGATGCTGCCGCAACTGGACGGG
CAGATGGACGCCTGGTTTCTCGACGGTTTTGCGCCGGCGAAAAACCCGGAAATGTGGACTCCGGAGCTGTTTGCCGAACT
GGCGCGCCTGTCCACATCGAGCACCACGATTGGTACGTTTACCAGCACCGGCTGGGTGCGCCGCTCGCTGAATGCGGCAG
GTTTCAAGATGAAGCGCGTGCCGGGCATCGGCCACAAATGGGAAGTGCTGCGTGGCACGTTTATCGCCTGGCCAGAGGAC
GTGGCGCATCCGCCTGCCGCCAAGCCATGGTTAGCCCGCCCCGCGCCGATTGGCGGCGAGCGCAAAGCGCTGGTGATTGG
CGCGGGTCTGGCCGGCTGTGCCACGGCGCAGAGTCTGGCGCAACGCGGCTGGCAGGTCAGCCTGCTGGAGCGCCACGCCG
CACCGGCGCAGGAAGCGTCGGGTAATCCGCAAGGCGTGCTGTATTTGAAACTGTCGGCGCACGGTACCGCGCTGTCACAG
TTGATCCTCAGCGGTTTTGGCCATACGCGAAGGCTGCTTGAACGCTTGCAGCGCGGGGTCGACTGGGACGCTTGCGGGGT
GCTGCAACTGACCTTCGATGACAAGGAAGCGCTGCGCCAGAAACAACTGGCCGATGCCTTCCCGGAATCACTTCTGCACC
TGCTGGACAAGCCTGCCGCCGAAGCACAAAGCGGCGTTGCACTGAACAGTGGCGGGCTGTTTTATCCCGAAGGCGGCTGG
GTTCATCCGCCTGCGTTGTGTCACGCACAGGCTGCGCACGCCAATATCCGCCTGATCGCTCATCAGCAGGCGCTGGAGCT
GAGACGTGTCGATGATCAGTGGCAGGTCTGGAGTGAGGAACAGTTGGTCGACAGCGCTCCGGTCGTTGTGCTGGCCGGTG
CAGCTGATATCCAGCAGTTTTCGCAGAGCGCCGATTTGCCGCTCAAGCGCATTCGCGGGCAGATCACCCGCCTGCCACAG
ACTCAGGCCAGCACGGCATTGCGCAGCGTGGTGTGCGCCGAGGGTTATGTGGCTCCGGCGCGTCTGGGCGAGCATACGCT
CGGGGCGAGTTTTGACTTCAACAGCACTGATCTGACGCCAAACCTGGCCGATCACCTGGACAATCTGGGGCTGTTGCGGG
AGATTTCGGAAGACCTCACTTCGCGCCTCGATACCGCCGATTTGTCGCCCGAGCAATTGCAGGGACGCGCGGCGTTTCGC
TGCACCAGCCCGGATTACCTGCCCATCGTCGGGCCGCTGGCCGATCGCGAGGCGTTCATGCAAGCCTATGCCGCGCTGGG
CAAGGATGCGCGTCAGGTGCCGGACATCACCTGCCCGTGGCTGGATGGCCTGTACGTTAACAGCGGCCACGGTTCGCGCG
GCCTTATCACTGCGCCGCTGTGTGGCGAACTGATCGCCGCCTGGCTCGACAACGAACCACTGCCCCTGCCACGCAGCGTC
GCCGAGGCCTGCCACCCCAACCGCTTTGCCCTGCGCGGGCTGATTCGCGGCAAGGGGAAACAGACTGTCGGGCACTGA

Upstream 100 bases:

>100_bases
AGACCATTGATGACCGGCATCGGTCAATCCACACAACCTTGATAGCACTTTTCTCTACGCAGACGGCTAAAATGCCGCCA
CTGCCTCCGCTGAGCCCGAC

Downstream 100 bases:

>100_bases
AACCCGCAGATCATCCTCGCTTGCACGACAGTTGAGATTAGCGTTCCCTACGCTCCAGTGGAATGCAGCTCTCGACGCTC
TGCGTCGAAAAGAGGACGCA

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MTITRHAQIDWDEHGNPRSRDFSDVYFSTESGLEETRHVFLVQNDLRRRFTELQDGGRLIIGETGFGTGLNFLCAWQLFD
ECAPANARLQFVSVEKYPLSHADLQRALALWPELSPFAGQLLDQYVAVHEGFQRLVFAGGRVTLTLLIGDALQMLPQLDG
QMDAWFLDGFAPAKNPEMWTPELFAELARLSTSSTTIGTFTSTGWVRRSLNAAGFKMKRVPGIGHKWEVLRGTFIAWPED
VAHPPAAKPWLARPAPIGGERKALVIGAGLAGCATAQSLAQRGWQVSLLERHAAPAQEASGNPQGVLYLKLSAHGTALSQ
LILSGFGHTRRLLERLQRGVDWDACGVLQLTFDDKEALRQKQLADAFPESLLHLLDKPAAEAQSGVALNSGGLFYPEGGW
VHPPALCHAQAAHANIRLIAHQQALELRRVDDQWQVWSEEQLVDSAPVVVLAGAADIQQFSQSADLPLKRIRGQITRLPQ
TQASTALRSVVCAEGYVAPARLGEHTLGASFDFNSTDLTPNLADHLDNLGLLREISEDLTSRLDTADLSPEQLQGRAAFR
CTSPDYLPIVGPLADREAFMQAYAALGKDARQVPDITCPWLDGLYVNSGHGSRGLITAPLCGELIAAWLDNEPLPLPRSV
AEACHPNRFALRGLIRGKGKQTVGH

Sequences:

>Translated_665_residues
MTITRHAQIDWDEHGNPRSRDFSDVYFSTESGLEETRHVFLVQNDLRRRFTELQDGGRLIIGETGFGTGLNFLCAWQLFD
ECAPANARLQFVSVEKYPLSHADLQRALALWPELSPFAGQLLDQYVAVHEGFQRLVFAGGRVTLTLLIGDALQMLPQLDG
QMDAWFLDGFAPAKNPEMWTPELFAELARLSTSSTTIGTFTSTGWVRRSLNAAGFKMKRVPGIGHKWEVLRGTFIAWPED
VAHPPAAKPWLARPAPIGGERKALVIGAGLAGCATAQSLAQRGWQVSLLERHAAPAQEASGNPQGVLYLKLSAHGTALSQ
LILSGFGHTRRLLERLQRGVDWDACGVLQLTFDDKEALRQKQLADAFPESLLHLLDKPAAEAQSGVALNSGGLFYPEGGW
VHPPALCHAQAAHANIRLIAHQQALELRRVDDQWQVWSEEQLVDSAPVVVLAGAADIQQFSQSADLPLKRIRGQITRLPQ
TQASTALRSVVCAEGYVAPARLGEHTLGASFDFNSTDLTPNLADHLDNLGLLREISEDLTSRLDTADLSPEQLQGRAAFR
CTSPDYLPIVGPLADREAFMQAYAALGKDARQVPDITCPWLDGLYVNSGHGSRGLITAPLCGELIAAWLDNEPLPLPRSV
AEACHPNRFALRGLIRGKGKQTVGH
>Mature_664_residues
TITRHAQIDWDEHGNPRSRDFSDVYFSTESGLEETRHVFLVQNDLRRRFTELQDGGRLIIGETGFGTGLNFLCAWQLFDE
CAPANARLQFVSVEKYPLSHADLQRALALWPELSPFAGQLLDQYVAVHEGFQRLVFAGGRVTLTLLIGDALQMLPQLDGQ
MDAWFLDGFAPAKNPEMWTPELFAELARLSTSSTTIGTFTSTGWVRRSLNAAGFKMKRVPGIGHKWEVLRGTFIAWPEDV
AHPPAAKPWLARPAPIGGERKALVIGAGLAGCATAQSLAQRGWQVSLLERHAAPAQEASGNPQGVLYLKLSAHGTALSQL
ILSGFGHTRRLLERLQRGVDWDACGVLQLTFDDKEALRQKQLADAFPESLLHLLDKPAAEAQSGVALNSGGLFYPEGGWV
HPPALCHAQAAHANIRLIAHQQALELRRVDDQWQVWSEEQLVDSAPVVVLAGAADIQQFSQSADLPLKRIRGQITRLPQT
QASTALRSVVCAEGYVAPARLGEHTLGASFDFNSTDLTPNLADHLDNLGLLREISEDLTSRLDTADLSPEQLQGRAAFRC
TSPDYLPIVGPLADREAFMQAYAALGKDARQVPDITCPWLDGLYVNSGHGSRGLITAPLCGELIAAWLDNEPLPLPRSVA
EACHPNRFALRGLIRGKGKQTVGH

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=676, Percent_Identity=39.3491124260355, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_PSEU2 (Q4ZPZ9)

Other databases:

- EMBL:   CP000075
- RefSeq:   YP_236811.1
- ProteinModelPortal:   Q4ZPZ9
- SMR:   Q4ZPZ9
- STRING:   Q4ZPZ9
- GeneID:   3369277
- GenomeReviews:   CP000075_GR
- KEGG:   psb:Psyr_3742
- NMPDR:   fig|205918.4.peg.4178
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- ProtClustDB:   PRK01747
- BioCyc:   PSYR205918:PSYR_3742-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 72739; Mature: 72608

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITRHAQIDWDEHGNPRSRDFSDVYFSTESGLEETRHVFLVQNDLRRRFTELQDGGRLI
CCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHEEEEEHHHHHHHHHHHCCCCEEE
IGETGFGTGLNFLCAWQLFDECAPANARLQFVSVEKYPLSHADLQRALALWPELSPFAGQ
EECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHH
LLDQYVAVHEGFQRLVFAGGRVTLTLLIGDALQMLPQLDGQMDAWFLDGFAPAKNPEMWT
HHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCC
PELFAELARLSTSSTTIGTFTSTGWVRRSLNAAGFKMKRVPGIGHKWEVLRGTFIAWPED
HHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCCCEEEECCCCCCCCHHHHHCEEEECCHH
VAHPPAAKPWLARPAPIGGERKALVIGAGLAGCATAQSLAQRGWQVSLLERHAAPAQEAS
HCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEHHHHCCCCCHHCC
GNPQGVLYLKLSAHGTALSQLILSGFGHTRRLLERLQRGVDWDACGVLQLTFDDKEALRQ
CCCCEEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHH
KQLADAFPESLLHLLDKPAAEAQSGVALNSGGLFYPEGGWVHPPALCHAQAAHANIRLIA
HHHHHHHHHHHHHHHCCCHHHHHCCEEECCCCEECCCCCCCCCHHHHHHHHCCCEEEEEE
HQQALELRRVDDQWQVWSEEQLVDSAPVVVLAGAADIQQFSQSADLPLKRIRGQITRLPQ
HHHHHHHHHCCHHHHHCCHHHHHCCCCEEEEECCHHHHHHHHCCCCCHHHHHHHHHCCCC
TQASTALRSVVCAEGYVAPARLGEHTLGASFDFNSTDLTPNLADHLDNLGLLREISEDLT
HHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SRLDTADLSPEQLQGRAAFRCTSPDYLPIVGPLADREAFMQAYAALGKDARQVPDITCPW
HHCCCCCCCHHHHCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHCCCHHHCCCCCCCC
LDGLYVNSGHGSRGLITAPLCGELIAAWLDNEPLPLPRSVAEACHPNRFALRGLIRGKGK
CCCEEEECCCCCCCEEECCHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCC
QTVGH
CCCCC
>Mature Secondary Structure 
TITRHAQIDWDEHGNPRSRDFSDVYFSTESGLEETRHVFLVQNDLRRRFTELQDGGRLI
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHEEEEEHHHHHHHHHHHCCCCEEE
IGETGFGTGLNFLCAWQLFDECAPANARLQFVSVEKYPLSHADLQRALALWPELSPFAGQ
EECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHH
LLDQYVAVHEGFQRLVFAGGRVTLTLLIGDALQMLPQLDGQMDAWFLDGFAPAKNPEMWT
HHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCC
PELFAELARLSTSSTTIGTFTSTGWVRRSLNAAGFKMKRVPGIGHKWEVLRGTFIAWPED
HHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCCCEEEECCCCCCCCHHHHHCEEEECCHH
VAHPPAAKPWLARPAPIGGERKALVIGAGLAGCATAQSLAQRGWQVSLLERHAAPAQEAS
HCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEHHHHCCCCCHHCC
GNPQGVLYLKLSAHGTALSQLILSGFGHTRRLLERLQRGVDWDACGVLQLTFDDKEALRQ
CCCCEEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHH
KQLADAFPESLLHLLDKPAAEAQSGVALNSGGLFYPEGGWVHPPALCHAQAAHANIRLIA
HHHHHHHHHHHHHHHCCCHHHHHCCEEECCCCEECCCCCCCCCHHHHHHHHCCCEEEEEE
HQQALELRRVDDQWQVWSEEQLVDSAPVVVLAGAADIQQFSQSADLPLKRIRGQITRLPQ
HHHHHHHHHCCHHHHHCCHHHHHCCCCEEEEECCHHHHHHHHCCCCCHHHHHHHHHCCCC
TQASTALRSVVCAEGYVAPARLGEHTLGASFDFNSTDLTPNLADHLDNLGLLREISEDLT
HHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SRLDTADLSPEQLQGRAAFRCTSPDYLPIVGPLADREAFMQAYAALGKDARQVPDITCPW
HHCCCCCCCHHHHCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHCCCHHHCCCCCCCC
LDGLYVNSGHGSRGLITAPLCGELIAAWLDNEPLPLPRSVAEACHPNRFALRGLIRGKGK
CCCEEEECCCCCCCEEECCHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCC
QTVGH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA