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The map label for this gene is flgJ [H]

Identifier: 66046701

GI number: 66046701

Start: 4142461

End: 4143705

Strand: Reverse

Name: flgJ [H]

Synonym: Psyr_3472

Alternate gene names: 66046701

Gene position: 4143705-4142461 (Counterclockwise)

Preceding gene: 66046702

Following gene: 66046700

Centisome position: 68.0

GC content: 61.29

Gene sequence:

>1245_bases
ATGGATATGCCCAAGAGCGGAATTTCGTCAGCGGTCGACTCGGGTGCGTACACCGACGTCAACCGTCTGGCGTCGCTGAA
GCATGGCGACAAGGACAGTGTGGAAAACCAGAAGAAGGTGGCCCGCGAGTTCGAGTCGCTGTTCGTCAGCCAGATGCTCA
AGGCCATGCGCTCGGCCAACGAAGTGCTGGCCAAGGACAATCCGATGAACACCCCGGCCACGCGGCAGTATCAGGATATG
TATGACCAGCAGTTGGCGGTCACGCTGTCCACGCGTGGCAACGGTATCGGTCTGCAGGATGTGCTGATGCGCCAATTGTC
GAAAGACAAGGGGATAAACCATGCCGCGCCGGTCAATACGACCGACGCTGCAACGGCCGCCACTGACGCTGCGCCGGCCA
AGACCGGTCTGGCGACCAGCGTCTACCAGCGTCCGTTGTGGGCCACGCGCTCGGTGGCTGCCGATCAGGCGGCGGCTGCG
GCATCGGCGTCCGGAGAAGGGCGCAATGACATGGCGATGCTCAACGCGCGCCGTCTGTCCCTGCCCGCCAAGCTCACCGA
TCGTCTGCTGGCTGGCATCGTGCCGTCCGCCACAACGGCGGTCAGCAACAGTCCGGTACCCGCTCGCGCGACAGCCGGCA
CTACAGGCAACAACGGCGACTGGACACTCGATCCGAACCTGGCACCCGCGCCCGAGTACGTGCGCTCCATGGCCCAGCCA
CCGCTGGCACCGGCCAAGCGTGCGTTCAGCAACGCCGATCAGTTCGTCGAAACCATGCTGCCATTGGCCAAGGAAGCCGC
AGCACGTATCGGCGTTGACCCGGTCATGCTGGTGGCGCAGGCGGCACTGGAAACCGGTTGGGGCAAATCGATCATGCGTC
AGCAGGACGGCAGCAGCAGTCATAACCTGTTCGGCATCAAGGCGGCAGGCAGCTGGAAGGGGGCCGAAGCGCGCGCGATC
ACCAGCGAGTTCCGCGACGGCAAGATGGTCAAGGAAACCGCGGACTTCCGTTCCTACGATTCCTATGCCGACAGCTTCCA
CGACCTGGTCAGTCTGTTGCAGAACAACAGCCGCTATAAAGAAGTTGTCAATTCGGCCGATAAACCGGAACAGTTTGTAA
AAGAATTGCAGAAAGCCGGTTACGCCACTGACCCGGACTATGCCAGCAAGATTTCGCAGATTGCCAAGCAGATGAAGAGT
TACCAGACTTACGCCGCTGCAGCGGGCTCTTCCACGACTTTATAA

Upstream 100 bases:

>100_bases
CAGGTGGGCGCTGCGCCCGGTGACCTGATGGCCATTCTCGAAGCGCTTAAACAGGCCGGCGCGTTGCAAGCCGACCTGAT
CGTGATCTGAGGATTCGAGC

Downstream 100 bases:

>100_bases
GGTTTGACTCATGTCGCTGATTTCAATTGGGCTCTCAGGTATCAATGCCAGCAGTGCCGCGATCAACACCATCGGTAACA
ACACGGCCAACGTGGATACC

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MDMPKSGISSAVDSGAYTDVNRLASLKHGDKDSVENQKKVAREFESLFVSQMLKAMRSANEVLAKDNPMNTPATRQYQDM
YDQQLAVTLSTRGNGIGLQDVLMRQLSKDKGINHAAPVNTTDAATAATDAAPAKTGLATSVYQRPLWATRSVAADQAAAA
ASASGEGRNDMAMLNARRLSLPAKLTDRLLAGIVPSATTAVSNSPVPARATAGTTGNNGDWTLDPNLAPAPEYVRSMAQP
PLAPAKRAFSNADQFVETMLPLAKEAAARIGVDPVMLVAQAALETGWGKSIMRQQDGSSSHNLFGIKAAGSWKGAEARAI
TSEFRDGKMVKETADFRSYDSYADSFHDLVSLLQNNSRYKEVVNSADKPEQFVKELQKAGYATDPDYASKISQIAKQMKS
YQTYAAAAGSSTTL

Sequences:

>Translated_414_residues
MDMPKSGISSAVDSGAYTDVNRLASLKHGDKDSVENQKKVAREFESLFVSQMLKAMRSANEVLAKDNPMNTPATRQYQDM
YDQQLAVTLSTRGNGIGLQDVLMRQLSKDKGINHAAPVNTTDAATAATDAAPAKTGLATSVYQRPLWATRSVAADQAAAA
ASASGEGRNDMAMLNARRLSLPAKLTDRLLAGIVPSATTAVSNSPVPARATAGTTGNNGDWTLDPNLAPAPEYVRSMAQP
PLAPAKRAFSNADQFVETMLPLAKEAAARIGVDPVMLVAQAALETGWGKSIMRQQDGSSSHNLFGIKAAGSWKGAEARAI
TSEFRDGKMVKETADFRSYDSYADSFHDLVSLLQNNSRYKEVVNSADKPEQFVKELQKAGYATDPDYASKISQIAKQMKS
YQTYAAAAGSSTTL
>Mature_414_residues
MDMPKSGISSAVDSGAYTDVNRLASLKHGDKDSVENQKKVAREFESLFVSQMLKAMRSANEVLAKDNPMNTPATRQYQDM
YDQQLAVTLSTRGNGIGLQDVLMRQLSKDKGINHAAPVNTTDAATAATDAAPAKTGLATSVYQRPLWATRSVAADQAAAA
ASASGEGRNDMAMLNARRLSLPAKLTDRLLAGIVPSATTAVSNSPVPARATAGTTGNNGDWTLDPNLAPAPEYVRSMAQP
PLAPAKRAFSNADQFVETMLPLAKEAAARIGVDPVMLVAQAALETGWGKSIMRQQDGSSSHNLFGIKAAGSWKGAEARAI
TSEFRDGKMVKETADFRSYDSYADSFHDLVSLLQNNSRYKEVVNSADKPEQFVKELQKAGYATDPDYASKISQIAKQMKS
YQTYAAAAGSSTTL

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG3951

COG function: function code MNO; Rod binding protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=141, Percent_Identity=46.0992907801418, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR019301
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 44196; Mature: 44196

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMPKSGISSAVDSGAYTDVNRLASLKHGDKDSVENQKKVAREFESLFVSQMLKAMRSAN
CCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVLAKDNPMNTPATRQYQDMYDQQLAVTLSTRGNGIGLQDVLMRQLSKDKGINHAAPVNT
HHHHCCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
TDAATAATDAAPAKTGLATSVYQRPLWATRSVAADQAAAAASASGEGRNDMAMLNARRLS
CCHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
LPAKLTDRLLAGIVPSATTAVSNSPVPARATAGTTGNNGDWTLDPNLAPAPEYVRSMAQP
CCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHCCC
PLAPAKRAFSNADQFVETMLPLAKEAAARIGVDPVMLVAQAALETGWGKSIMRQQDGSSS
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCC
HNLFGIKAAGSWKGAEARAITSEFRDGKMVKETADFRSYDSYADSFHDLVSLLQNNSRYK
CCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
EVVNSADKPEQFVKELQKAGYATDPDYASKISQIAKQMKSYQTYAAAAGSSTTL
HHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDMPKSGISSAVDSGAYTDVNRLASLKHGDKDSVENQKKVAREFESLFVSQMLKAMRSAN
CCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVLAKDNPMNTPATRQYQDMYDQQLAVTLSTRGNGIGLQDVLMRQLSKDKGINHAAPVNT
HHHHCCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
TDAATAATDAAPAKTGLATSVYQRPLWATRSVAADQAAAAASASGEGRNDMAMLNARRLS
CCHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
LPAKLTDRLLAGIVPSATTAVSNSPVPARATAGTTGNNGDWTLDPNLAPAPEYVRSMAQP
CCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHCCC
PLAPAKRAFSNADQFVETMLPLAKEAAARIGVDPVMLVAQAALETGWGKSIMRQQDGSSS
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCC
HNLFGIKAAGSWKGAEARAITSEFRDGKMVKETADFRSYDSYADSFHDLVSLLQNNSRYK
CCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
EVVNSADKPEQFVKELQKAGYATDPDYASKISQIAKQMKSYQTYAAAAGSSTTL
HHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]