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The map label for this gene is phoB [H]

Identifier: 66046680

GI number: 66046680

Start: 4114333

End: 4116039

Strand: Reverse

Name: phoB [H]

Synonym: Psyr_3451

Alternate gene names: 66046680

Gene position: 4116039-4114333 (Counterclockwise)

Preceding gene: 66046681

Following gene: 66046679

Centisome position: 67.55

GC content: 61.39

Gene sequence:

>1707_bases
GTGCAGCCAGCCCCGAACAAACTGTGCATTCTGATCGCTGATGACAGCACCAGTGACCGCTTGCTGCTGTCAACCATCGT
GGCGCGGCAGGGTCACCGGGTCCTCAGCGCGGGCAACGGTGTCGAAGCGGTTGCCATCTTCGAGGCCGAAAGCCCGCAGT
TGATTCTGATGGACGCCATGATGCCGGTCATGGATGGTTTCGAGGCCGCGCGAAGGATCAAGGCGATGGCCGGCGAATCG
CTGGTGCCGATCATTTTCCTGACCTCGCTCACCGAAGGCGAGGCGCTTGCACGCTGCCTGGACGCTGGTGGTGACGACTT
TATGTCCAAGCCTTACAACCCGCTGGTATTGGCCGCCAAGCTCAACGCCATGAACCGTTTGCGGGTATTGCACGAGACTG
TTCGCCAGCAGCGTGACCAGATCGCCAGGCACCATGAATATCTGCTCAATGAGCAACGTGTCGCCAAGGCGGTGTTCGAT
CAGATCGCCCACGCCGGTTGCCTGGGGGCGAGCAATATCCGCTACCTGCAGTCCCCCTACGCACTGTTCAACGGCGACCT
GATGCTGGCTGCTTACACCCCCGCCGGGCACATGCAGGTGCTGCTCGGTGATTTCACCGGACATGGCTTGCCGGCTGCCG
TTGGCGCGATGCCGCTTGCAGAAGTGTTCTATGGCATGACAGCCAAGGGCTATGGCCTGGTTGAAATCCTCCGTGAAATG
AACGCCAAGCTCAAACGCATCCTCCCGGTCGACATGTTTTGTTGTGCGACGCTGCTGTGTATCAATCTGCCGCAGCGGCA
GGTCGAGGTCTGGAGCGGTGGCTTGCCGGACGGCTACCTGCTGCGCGGCGCACAGGGCGAGCATGTGCCGCTGGCCTCGA
ATCATCTGCCCTTGGGCGTATTGAGCGTCGATGCCTTCGACGCGCGCACCGACGTGTATCCGTTGGCGCCGGGTGACCGC
ATATTTCTGTTGTCCGACGGCGTGCTCGACACCAGTAACAGTCAGGAGGAGCTGTTCGGTGCGCAGCGTCTGCGACAGGT
TCTGTCGGCCAATCGCGAACCGCAGTTGCTGATCGAAGAGATCCTCGAGGCGCTCAACGAATTTGGCGGCAAGGCGCGTG
ACGATGTCAGTCTGCTGGAAGTCAGCGCTGTCGAAGCGCCGCTGCAAAGTCTGCCTGCCATGATGTATTCGGACAGCGGC
GCCTCCAGCCCGCTGGACTGGTCGGCCAGCTTCGAGTTTCGCGCCTCGACCCTCAAGCGTTTCAACCCCCTGCCTTACGT
ACTGCAATTGCTGCTGGAGGTTCACGGCCTGCGTGATCAGGGCGCTGCACTGCATATGGTGCTCGGCGAGCTGTACAGCA
ATGCGCTGGAGCATGGTGTGCTAGGGTTGGATTCGGCGCTCAAGCACGACGCAGCAGGCTTCGCGCTCTATTATCAGCAG
CGGGCAGAACGCCTCGACAGGCTGCAGTCCGGCTTCATCCGCATGACCCTGCAGGTTGAAACGGTCGCGCAGGGCGGTCG
GCTGACCCTCGGTGTCGAGGACAGCGGCCAGGGATTTGATGTGGAAAAGACCCGGACGCTGACGCCTGCCGGCAATGAAC
TGTATGGTCGCGGGCTGCATCTGGTCTGTGAGTTGAGTCGCGAGGCCAGGTGGTCCAGGGATGGCAGGACCGCCTGCGTG
GAATTTTCCTGGGAGGGAGTGGCATAA

Upstream 100 bases:

>100_bases
GGTGACAAGGCATTGGTACGCCTGGTCAACTGCACGCCTGATGTGCTGAAGATTCTGGCCATTTCCAATTTCTCGAAGCT
GTTCGAATTGAGCATACCCA

Downstream 100 bases:

>100_bases
TCCCCGCCTGGAGTCTTGATCAAGGAGTGAACAAGTGTCGATTCATCTGGATTACAGCGTGTTGAATACCTTGCAGGAGG
TCATGGAAGACGAATATCCA

Product: response regulator receiver:ATP-binding region, ATPase-like:stage II sporulation E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MQPAPNKLCILIADDSTSDRLLLSTIVARQGHRVLSAGNGVEAVAIFEAESPQLILMDAMMPVMDGFEAARRIKAMAGES
LVPIIFLTSLTEGEALARCLDAGGDDFMSKPYNPLVLAAKLNAMNRLRVLHETVRQQRDQIARHHEYLLNEQRVAKAVFD
QIAHAGCLGASNIRYLQSPYALFNGDLMLAAYTPAGHMQVLLGDFTGHGLPAAVGAMPLAEVFYGMTAKGYGLVEILREM
NAKLKRILPVDMFCCATLLCINLPQRQVEVWSGGLPDGYLLRGAQGEHVPLASNHLPLGVLSVDAFDARTDVYPLAPGDR
IFLLSDGVLDTSNSQEELFGAQRLRQVLSANREPQLLIEEILEALNEFGGKARDDVSLLEVSAVEAPLQSLPAMMYSDSG
ASSPLDWSASFEFRASTLKRFNPLPYVLQLLLEVHGLRDQGAALHMVLGELYSNALEHGVLGLDSALKHDAAGFALYYQQ
RAERLDRLQSGFIRMTLQVETVAQGGRLTLGVEDSGQGFDVEKTRTLTPAGNELYGRGLHLVCELSREARWSRDGRTACV
EFSWEGVA

Sequences:

>Translated_568_residues
MQPAPNKLCILIADDSTSDRLLLSTIVARQGHRVLSAGNGVEAVAIFEAESPQLILMDAMMPVMDGFEAARRIKAMAGES
LVPIIFLTSLTEGEALARCLDAGGDDFMSKPYNPLVLAAKLNAMNRLRVLHETVRQQRDQIARHHEYLLNEQRVAKAVFD
QIAHAGCLGASNIRYLQSPYALFNGDLMLAAYTPAGHMQVLLGDFTGHGLPAAVGAMPLAEVFYGMTAKGYGLVEILREM
NAKLKRILPVDMFCCATLLCINLPQRQVEVWSGGLPDGYLLRGAQGEHVPLASNHLPLGVLSVDAFDARTDVYPLAPGDR
IFLLSDGVLDTSNSQEELFGAQRLRQVLSANREPQLLIEEILEALNEFGGKARDDVSLLEVSAVEAPLQSLPAMMYSDSG
ASSPLDWSASFEFRASTLKRFNPLPYVLQLLLEVHGLRDQGAALHMVLGELYSNALEHGVLGLDSALKHDAAGFALYYQQ
RAERLDRLQSGFIRMTLQVETVAQGGRLTLGVEDSGQGFDVEKTRTLTPAGNELYGRGLHLVCELSREARWSRDGRTACV
EFSWEGVA
>Mature_568_residues
MQPAPNKLCILIADDSTSDRLLLSTIVARQGHRVLSAGNGVEAVAIFEAESPQLILMDAMMPVMDGFEAARRIKAMAGES
LVPIIFLTSLTEGEALARCLDAGGDDFMSKPYNPLVLAAKLNAMNRLRVLHETVRQQRDQIARHHEYLLNEQRVAKAVFD
QIAHAGCLGASNIRYLQSPYALFNGDLMLAAYTPAGHMQVLLGDFTGHGLPAAVGAMPLAEVFYGMTAKGYGLVEILREM
NAKLKRILPVDMFCCATLLCINLPQRQVEVWSGGLPDGYLLRGAQGEHVPLASNHLPLGVLSVDAFDARTDVYPLAPGDR
IFLLSDGVLDTSNSQEELFGAQRLRQVLSANREPQLLIEEILEALNEFGGKARDDVSLLEVSAVEAPLQSLPAMMYSDSG
ASSPLDWSASFEFRASTLKRFNPLPYVLQLLLEVHGLRDQGAALHMVLGELYSNALEHGVLGLDSALKHDAAGFALYYQQ
RAERLDRLQSGFIRMTLQVETVAQGGRLTLGVEDSGQGFDVEKTRTLTPAGNELYGRGLHLVCELSREARWSRDGRTACV
EFSWEGVA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=104, Percent_Identity=36.5384615384615, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=34.453781512605, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=134, Percent_Identity=31.3432835820896, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=29.0598290598291, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 62030; Mature: 62030

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPAPNKLCILIADDSTSDRLLLSTIVARQGHRVLSAGNGVEAVAIFEAESPQLILMDAM
CCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCEEEHHHH
MPVMDGFEAARRIKAMAGESLVPIIFLTSLTEGEALARCLDAGGDDFMSKPYNPLVLAAK
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHCCCCCCEEEEEH
LNAMNRLRVLHETVRQQRDQIARHHEYLLNEQRVAKAVFDQIAHAGCLGASNIRYLQSPY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCC
ALFNGDLMLAAYTPAGHMQVLLGDFTGHGLPAAVGAMPLAEVFYGMTAKGYGLVEILREM
CEECCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
NAKLKRILPVDMFCCATLLCINLPQRQVEVWSGGLPDGYLLRGAQGEHVPLASNHLPLGV
HHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEE
LSVDAFDARTDVYPLAPGDRIFLLSDGVLDTSNSQEELFGAQRLRQVLSANREPQLLIEE
EEECCCCCCCCEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
ILEALNEFGGKARDDVSLLEVSAVEAPLQSLPAMMYSDSGASSPLDWSASFEFRASTLKR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCCCCHHHHHHHHH
FNPLPYVLQLLLEVHGLRDQGAALHMVLGELYSNALEHGVLGLDSALKHDAAGFALYYQQ
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHH
RAERLDRLQSGFIRMTLQVETVAQGGRLTLGVEDSGQGFDVEKTRTLTPAGNELYGRGLH
HHHHHHHHHCCEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCEEECCCCCCHHCCCEE
LVCELSREARWSRDGRTACVEFSWEGVA
EEEEECHHHCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MQPAPNKLCILIADDSTSDRLLLSTIVARQGHRVLSAGNGVEAVAIFEAESPQLILMDAM
CCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCEEEHHHH
MPVMDGFEAARRIKAMAGESLVPIIFLTSLTEGEALARCLDAGGDDFMSKPYNPLVLAAK
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHCCCCCCEEEEEH
LNAMNRLRVLHETVRQQRDQIARHHEYLLNEQRVAKAVFDQIAHAGCLGASNIRYLQSPY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCC
ALFNGDLMLAAYTPAGHMQVLLGDFTGHGLPAAVGAMPLAEVFYGMTAKGYGLVEILREM
CEECCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
NAKLKRILPVDMFCCATLLCINLPQRQVEVWSGGLPDGYLLRGAQGEHVPLASNHLPLGV
HHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEE
LSVDAFDARTDVYPLAPGDRIFLLSDGVLDTSNSQEELFGAQRLRQVLSANREPQLLIEE
EEECCCCCCCCEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
ILEALNEFGGKARDDVSLLEVSAVEAPLQSLPAMMYSDSGASSPLDWSASFEFRASTLKR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCCCCHHHHHHHHH
FNPLPYVLQLLLEVHGLRDQGAALHMVLGELYSNALEHGVLGLDSALKHDAAGFALYYQQ
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHH
RAERLDRLQSGFIRMTLQVETVAQGGRLTLGVEDSGQGFDVEKTRTLTPAGNELYGRGLH
HHHHHHHHHCCEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCEEECCCCCCHHCCCEE
LVCELSREARWSRDGRTACVEFSWEGVA
EEEEECHHHCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]