Click here to switch to the map view.

The map label for this gene is ada [H]

Identifier: 66046594

GI number: 66046594

Start: 4023774

End: 4024928

Strand: Reverse

Name: ada [H]

Synonym: Psyr_3365

Alternate gene names: 66046594

Gene position: 4024928-4023774 (Counterclockwise)

Preceding gene: 66046595

Following gene: 66046592

Centisome position: 66.05

GC content: 61.56

Gene sequence:

>1155_bases
GTGGCTCCGACAGGCGCAGGCTTATCGAAACTCAAGGCAGGCTCGACCATGGACAGCACAGAAGGCACTCAAACGGCACG
CGCCCTGCGCACTGAAGACGACCCGCGCTGGGACATCCTGGTCAATCGGCGTTCACACGCCGGCGCTGACTTCGTTTATG
GCGTGCTGACCACCGGCATCTATTGCAAGCCGTGCAGCCCGACCCGGCTGCCGCGTCCGGAAAACGTGGTGTTTTTTGAT
AGTGCGCACGACGCCGAGGCCGCCGGGTTTCGCCCATCCCTGCGCAATGCCGGCGACTCAAACGCGCTGCAACGCAAGCA
CGCAGAAGTCGTTGCCCAGGCCTGCAGACTGATCGATGCCGCCGATTCGATGCTCAATCTGACCGCGCTTGCGGAGCAGG
TCGGGATCAGCGGTTTTCATTTCCACCGCATTTTCAAAAGACTGACCGGCTTGACGCCCAGGGCCTACAGTGTCGCCTCG
TTACGTTCGCGGGTGAAAGTCCAGCTCTCACAGAGCGTAAGCATTACCCATGCGTTGTACGAGGCGGGCTACAACGCCAA
CAGTCGTTTCTACGAGGCTTCGCAGGACATGCTCGGCATGAAACCTTCCGAATACCGCGCGGGCGGCGCCAATGTCGATA
TCAGGTTCGCTCTGGGTGAAAGTTCGCTGGGTTCGATTCTGGTGGCCACCAGCAGCAAGGGTATCTGTGCGATTTCGCTC
GGTGATGACCCGCATGCGCTGATCGAAGCTTTCCAGGACCAGTTTCCCAACGCCAACCTGATCGGTGCAGACGCCGCGTT
CGAACAACTGGTTGCCGAGGTCGTGGGTTTTGTCGAATCACCTGCGACCGGCCTGGCGCTGCCATTGGATATCCGCGGCA
CGGTGTTTCAGGAGCGGGTCTGGCAGGCGCTGAGGGACATTCCTGCCGGATCGACGGCCACCTATACGCAGATCGCGACG
CAGATAGGACTGCCCAGTGCAGTCCGTGCGGTGGCCAACGCCTGCGGTGCGAACAAGCTCGCGGTGGCGATTCCCTGCCA
CCGCGTGGTGCGCAGCGATGGATCGTTGTCGGGCTACCGCTGGGGCGTCGAGCGCAAGCGCAAGCTGCTTGAAATCGAAG
CGGCCGAAGGGGCAGGGCTGTTTTCAGGGCGTTGA

Upstream 100 bases:

>100_bases
GCCTTACCGAACCATCGCTGCCTGGTATCTGTGGCGAGTACCGGTCGATTTCAGCGAGCTCGGCTCATCTCGAATTTGAA
AGCAAGACTCGGAGTGCCCA

Downstream 100 bases:

>100_bases
GCAGCAGCCCATTTTCTGCAGAATCCGATAGGCCAGTTTCACCCGCTCCTCGTTCGGGTAGTTTTTGTTGGCGAGGATGG
CAATCCCCAACTGCTGCTCC

Product: methylated-DNA-[protein]-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MAPTGAGLSKLKAGSTMDSTEGTQTARALRTEDDPRWDILVNRRSHAGADFVYGVLTTGIYCKPCSPTRLPRPENVVFFD
SAHDAEAAGFRPSLRNAGDSNALQRKHAEVVAQACRLIDAADSMLNLTALAEQVGISGFHFHRIFKRLTGLTPRAYSVAS
LRSRVKVQLSQSVSITHALYEAGYNANSRFYEASQDMLGMKPSEYRAGGANVDIRFALGESSLGSILVATSSKGICAISL
GDDPHALIEAFQDQFPNANLIGADAAFEQLVAEVVGFVESPATGLALPLDIRGTVFQERVWQALRDIPAGSTATYTQIAT
QIGLPSAVRAVANACGANKLAVAIPCHRVVRSDGSLSGYRWGVERKRKLLEIEAAEGAGLFSGR

Sequences:

>Translated_384_residues
MAPTGAGLSKLKAGSTMDSTEGTQTARALRTEDDPRWDILVNRRSHAGADFVYGVLTTGIYCKPCSPTRLPRPENVVFFD
SAHDAEAAGFRPSLRNAGDSNALQRKHAEVVAQACRLIDAADSMLNLTALAEQVGISGFHFHRIFKRLTGLTPRAYSVAS
LRSRVKVQLSQSVSITHALYEAGYNANSRFYEASQDMLGMKPSEYRAGGANVDIRFALGESSLGSILVATSSKGICAISL
GDDPHALIEAFQDQFPNANLIGADAAFEQLVAEVVGFVESPATGLALPLDIRGTVFQERVWQALRDIPAGSTATYTQIAT
QIGLPSAVRAVANACGANKLAVAIPCHRVVRSDGSLSGYRWGVERKRKLLEIEAAEGAGLFSGR
>Mature_383_residues
APTGAGLSKLKAGSTMDSTEGTQTARALRTEDDPRWDILVNRRSHAGADFVYGVLTTGIYCKPCSPTRLPRPENVVFFDS
AHDAEAAGFRPSLRNAGDSNALQRKHAEVVAQACRLIDAADSMLNLTALAEQVGISGFHFHRIFKRLTGLTPRAYSVASL
RSRVKVQLSQSVSITHALYEAGYNANSRFYEASQDMLGMKPSEYRAGGANVDIRFALGESSLGSILVATSSKGICAISLG
DDPHALIEAFQDQFPNANLIGADAAFEQLVAEVVGFVESPATGLALPLDIRGTVFQERVWQALRDIPAGSTATYTQIATQ
IGLPSAVRAVANACGANKLAVAIPCHRVVRSDGSLSGYRWGVERKRKLLEIEAAEGAGLFSGR

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=342, Percent_Identity=47.953216374269, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI1787596, Length=88, Percent_Identity=46.5909090909091, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=129, Percent_Identity=41.8604651162791, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI115533068, Length=130, Percent_Identity=42.3076923076923, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320001, Length=170, Percent_Identity=32.3529411764706, Blast_Score=92, Evalue=9e-20,
Organism=Drosophila melanogaster, GI17137554, Length=126, Percent_Identity=42.8571428571429, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 41026; Mature: 40895

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPTGAGLSKLKAGSTMDSTEGTQTARALRTEDDPRWDILVNRRSHAGADFVYGVLTTGI
CCCCCCCHHHHCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCE
YCKPCSPTRLPRPENVVFFDSAHDAEAAGFRPSLRNAGDSNALQRKHAEVVAQACRLIDA
EECCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ADSMLNLTALAEQVGISGFHFHRIFKRLTGLTPRAYSVASLRSRVKVQLSQSVSITHALY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
EAGYNANSRFYEASQDMLGMKPSEYRAGGANVDIRFALGESSLGSILVATSSKGICAISL
HCCCCCCCCHHHCCHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEE
GDDPHALIEAFQDQFPNANLIGADAAFEQLVAEVVGFVESPATGLALPLDIRGTVFQERV
CCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
WQALRDIPAGSTATYTQIATQIGLPSAVRAVANACGANKLAVAIPCHRVVRSDGSLSGYR
HHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCEE
WGVERKRKLLEIEAAEGAGLFSGR
CCHHHHHHEEEEECCCCCCCCCCC
>Mature Secondary Structure 
APTGAGLSKLKAGSTMDSTEGTQTARALRTEDDPRWDILVNRRSHAGADFVYGVLTTGI
CCCCCCHHHHCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCE
YCKPCSPTRLPRPENVVFFDSAHDAEAAGFRPSLRNAGDSNALQRKHAEVVAQACRLIDA
EECCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ADSMLNLTALAEQVGISGFHFHRIFKRLTGLTPRAYSVASLRSRVKVQLSQSVSITHALY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
EAGYNANSRFYEASQDMLGMKPSEYRAGGANVDIRFALGESSLGSILVATSSKGICAISL
HCCCCCCCCHHHCCHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEE
GDDPHALIEAFQDQFPNANLIGADAAFEQLVAEVVGFVESPATGLALPLDIRGTVFQERV
CCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
WQALRDIPAGSTATYTQIATQIGLPSAVRAVANACGANKLAVAIPCHRVVRSDGSLSGYR
HHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCEE
WGVERKRKLLEIEAAEGAGLFSGR
CCHHHHHHEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]