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The map label for this gene is wcaF [C]

Identifier: 66046539

GI number: 66046539

Start: 3961818

End: 3962639

Strand: Direct

Name: wcaF [C]

Synonym: Psyr_3310

Alternate gene names: 66046539

Gene position: 3961818-3962639 (Clockwise)

Preceding gene: 66046538

Following gene: 66046540

Centisome position: 65.02

GC content: 61.31

Gene sequence:

>822_bases
ATGAAACACCGCATGATGCATTCTCATAATCTCAGAGAGGCACTGCCCGATTACGCGCGCAAGATGGCGGTGCCGCTCGA
CGAGCTGGAAGCCGCCTACCAGTGGATGCTGGACAACGAAGTGTGCTTTGAAACACGTATCAAGGATCGCACCCTGTCGT
TCATCTGCTACCTCAATATCGAGCCCAGAATCGAACACCCGCTGGCGCGACGCTTCTACAAGCTGCTGGCCATCGAAACC
AAAGGCGCGCTTATCCCGCTGTACGGGATCAACTGGCCGACCCTGCGCGACCGCATGTTGCGGACTTGGGAGCAGGCTTA
CAACATCCTGATCTGCAAGATCCCCAGCCACACCCTGCGCCTGCTGTGGTTGCGCATCGGCGGCGCGAAGATCGGCAAGG
GCTCGACGGTCTGGCGCAACACCGAGGTGCTCGGCGTCGACAGCCTGCGCATCGGCAATGACAGCACGGTGGGCTGGCAT
TGCCAGCTTGACGCGCGCGGCGGGCTGGTGATCGGCGATCACGTCACCATCGCTTCGCATGTGCTGATCATCGCCGGCGG
CCACGATCTCAACGAACCCGAATTCTGGGCAGTAGGCGGGCCGGTGTTCATCGGCGACTACGCCTGGATCTGCAGCCGTG
CGCTGCTTTCGTTTGGTGCCGACATCGGCGAGGGCGCAGTGGTGGGCGGTAACAGCGTGGTCTCCAAACCGGTTCCGCCC
TATGCCATCGTCAGTGGCCCGAATGCGGAAATCAAGGGCGAGCGTGCTCGCGGCCTCAACTACAAGGTGGGCGGCAAAGG
CCTGTTCACCTTGTTTCACTGA

Upstream 100 bases:

>100_bases
TATTGGTCGGCATCAGCCTGCACGAAACCCGACGCCTGCGCGCCAGGACCGACACCCCATCCAGACACCTTGACGCCTCA
CCCGAGCCGGGAGCCTCCTC

Downstream 100 bases:

>100_bases
TCAGAAGCACAGACGATGCTGGGTTCGACACTTTGGCTGACACTGGCCACCCTCACCGGTCTGGCTGCCGGATTTGCCCG
CGAATGGCTGTTGGTGGCGG

Product: hexapaptide repeat-containing transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKHRMMHSHNLREALPDYARKMAVPLDELEAAYQWMLDNEVCFETRIKDRTLSFICYLNIEPRIEHPLARRFYKLLAIET
KGALIPLYGINWPTLRDRMLRTWEQAYNILICKIPSHTLRLLWLRIGGAKIGKGSTVWRNTEVLGVDSLRIGNDSTVGWH
CQLDARGGLVIGDHVTIASHVLIIAGGHDLNEPEFWAVGGPVFIGDYAWICSRALLSFGADIGEGAVVGGNSVVSKPVPP
YAIVSGPNAEIKGERARGLNYKVGGKGLFTLFH

Sequences:

>Translated_273_residues
MKHRMMHSHNLREALPDYARKMAVPLDELEAAYQWMLDNEVCFETRIKDRTLSFICYLNIEPRIEHPLARRFYKLLAIET
KGALIPLYGINWPTLRDRMLRTWEQAYNILICKIPSHTLRLLWLRIGGAKIGKGSTVWRNTEVLGVDSLRIGNDSTVGWH
CQLDARGGLVIGDHVTIASHVLIIAGGHDLNEPEFWAVGGPVFIGDYAWICSRALLSFGADIGEGAVVGGNSVVSKPVPP
YAIVSGPNAEIKGERARGLNYKVGGKGLFTLFH
>Mature_273_residues
MKHRMMHSHNLREALPDYARKMAVPLDELEAAYQWMLDNEVCFETRIKDRTLSFICYLNIEPRIEHPLARRFYKLLAIET
KGALIPLYGINWPTLRDRMLRTWEQAYNILICKIPSHTLRLLWLRIGGAKIGKGSTVWRNTEVLGVDSLRIGNDSTVGWH
CQLDARGGLVIGDHVTIASHVLIIAGGHDLNEPEFWAVGGPVFIGDYAWICSRALLSFGADIGEGAVVGGNSVVSKPVPP
YAIVSGPNAEIKGERARGLNYKVGGKGLFTLFH

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=136, Percent_Identity=30.8823529411765, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 30470; Mature: 30470

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHRMMHSHNLREALPDYARKMAVPLDELEAAYQWMLDNEVCFETRIKDRTLSFICYLNI
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEC
EPRIEHPLARRFYKLLAIETKGALIPLYGINWPTLRDRMLRTWEQAYNILICKIPSHTLR
CCCCCCHHHHHHHHHEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEECCCHHEE
LLWLRIGGAKIGKGSTVWRNTEVLGVDSLRIGNDSTVGWHCQLDARGGLVIGDHVTIASH
EEEEEECCCEECCCCEEEECCEEEEECEEEECCCCCEEEEEEEECCCCEEEECCEEEEEE
VLIIAGGHDLNEPEFWAVGGPVFIGDYAWICSRALLSFGADIGEGAVVGGNSVVSKPVPP
EEEEECCCCCCCCCEEEECCCEEECCHHHHHHHHHHHHCCCCCCCEEECCCCEECCCCCC
YAIVSGPNAEIKGERARGLNYKVGGKGLFTLFH
EEEEECCCCCCCCCCCCCCCEEECCCEEEEEEC
>Mature Secondary Structure
MKHRMMHSHNLREALPDYARKMAVPLDELEAAYQWMLDNEVCFETRIKDRTLSFICYLNI
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEC
EPRIEHPLARRFYKLLAIETKGALIPLYGINWPTLRDRMLRTWEQAYNILICKIPSHTLR
CCCCCCHHHHHHHHHEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEECCCHHEE
LLWLRIGGAKIGKGSTVWRNTEVLGVDSLRIGNDSTVGWHCQLDARGGLVIGDHVTIASH
EEEEEECCCEECCCCEEEECCEEEEECEEEECCCCCEEEEEEEECCCCEEEECCEEEEEE
VLIIAGGHDLNEPEFWAVGGPVFIGDYAWICSRALLSFGADIGEGAVVGGNSVVSKPVPP
EEEEECCCCCCCCCEEEECCCEEECCHHHHHHHHHHHHCCCCCCCEEECCCCEECCCCCC
YAIVSGPNAEIKGERARGLNYKVGGKGLFTLFH
EEEEECCCCCCCCCCCCCCCEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]