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The map label for this gene is manC [H]

Identifier: 66046531

GI number: 66046531

Start: 3951223

End: 3952680

Strand: Direct

Name: manC [H]

Synonym: Psyr_3302

Alternate gene names: 66046531

Gene position: 3951223-3952680 (Clockwise)

Preceding gene: 66046530

Following gene: 66046532

Centisome position: 64.84

GC content: 61.25

Gene sequence:

>1458_bases
ATGAGCACGCTCATTCCCTGCATCATTGCCGGCGGCTCCGGCACACGCCTGTGGCCGGTTTCGCGTGAAGCCATGCCCAA
GCCGTTCATGCGTCTGCCAGATGGCCAGAGCCTGCTGCAGAAGACCTTTCTGCGTGCAGTCGGCCTGCCCGGCGTCGAGC
GTCTGCTGACGGTGACCAACCGGGAAGTGTATTTCCGCACGGTGGACGACTACCGCCAGTTGAACAAGGGCAATGCACGC
CTGGATTTCATCCTTGAGCCTTTCGGCCGTAATACGGCAGCTGCGGTTGCCGCTGCGGCACTGCATGTCAGCCAGCTGTA
TGGCGACGAGGCGCAACTGCTGGTGTTGCCTGCCGATCACCTGATTACCGACGTGGCGGAATTCTCCCGAGCAGTGACGG
CGGCACAGAAGCTGGCCGCAGAGGGCTGGCTGGTGACGTTCGGCATCCTGCCGACCGCGCCGGAAACCGGTTTTGGCTAC
ATCGAGAAAGGCCCCGTACTCAATGCCGACGCCTGTCAGGTCGCCCGTTTTGTCGAAAAACCGGACGCGATCACTGCCCA
GGGCTATCTGGACAGTGGTCAGCATTTCTGGAATGCCGGCATGTTCTGCATGCGCGCCGACGCCGTGCTGCGCGAGTTGC
GCAAGCATGCACCGGACGTCCTGAGCGCCGTCAGCGCGTGCCTGGAATCGAGCCCGCGCAAGGAAGGCAACCGTGAGCTG
CAAGTCGAGCTGGACAGTGAGTCGTTTGCCCTGACACCGGACATTTCCATCGACTACGCGCTGATGGAACGCTCCGAAAA
AGTCGCCGTGGTGCCCTGCCAGCTTGGCTGGAGTGATATCGGCTCGTGGCAGGCGGTGCGTGAACTGTCTCCGGTCGATG
CTCAAGGCAACTACTGCAATGGCGAAACCGTGCTGCACGACGTCAGCAACTGCTACATCGACTCACCCAAGCGTCTGGTG
GGCGCTATCGGCCTGGACAATCTGATCATCATCGACACCCCCGACGCCTTGCTGATCGCCGACGGCGGCCGCAGCCAGGA
CGTCAGGATCATCGCTCAGGAGCTCAAGCGTCAGGGCCATGATGCCTATCGCCTGCACCGCACCGTTACACGCCCCTGGG
GCACCTACACCGTCCTCGAAGAAGGCAAGCGTTTCAAGATCAAGCGCATCGTAGTTCGTCCGCAGGCGTCCCTGTCGCTG
CAGATGCACCATCACCGCAGCGAACACTGGATCGTGGTCAGCGGCATGGCCCGTGTCACCAATGGCGAGCGTGAGTTCAT
GCTCGATACCAACGAGTCGACCTTCATCAAGCCGGGCCACACGCATCGTCTGGTCAACCCCGGCGTCATCGATCTGGTGA
TGATCGAAGTGCAGAGCGGCGAGTACCTGGGCGAAGACGACATCGTGCGTTTTACCGACATTTATGGACGTGTACCCGAT
AAGGCCATGAAGAGCTGA

Upstream 100 bases:

>100_bases
AGTTCGACCTTGAGTACATAGAAAACTGGTCCGTCTGGTTCGACCTCTACATCCTTATGCGCACCGTGCCTGCAGTGCTA
TTCACCCGTGAGGCTTACTG

Downstream 100 bases:

>100_bases
TAGGCGCAGCAACAAGCGCCGCTTGATTCAAGCGGCACGCAATCGACAGCACCCGGAACAGGCATGACCCGCCAACATGA
ATTTATTACCCGCGAGGGAG

Product: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSTLIPCIIAGGSGTRLWPVSREAMPKPFMRLPDGQSLLQKTFLRAVGLPGVERLLTVTNREVYFRTVDDYRQLNKGNAR
LDFILEPFGRNTAAAVAAAALHVSQLYGDEAQLLVLPADHLITDVAEFSRAVTAAQKLAAEGWLVTFGILPTAPETGFGY
IEKGPVLNADACQVARFVEKPDAITAQGYLDSGQHFWNAGMFCMRADAVLRELRKHAPDVLSAVSACLESSPRKEGNREL
QVELDSESFALTPDISIDYALMERSEKVAVVPCQLGWSDIGSWQAVRELSPVDAQGNYCNGETVLHDVSNCYIDSPKRLV
GAIGLDNLIIIDTPDALLIADGGRSQDVRIIAQELKRQGHDAYRLHRTVTRPWGTYTVLEEGKRFKIKRIVVRPQASLSL
QMHHHRSEHWIVVSGMARVTNGEREFMLDTNESTFIKPGHTHRLVNPGVIDLVMIEVQSGEYLGEDDIVRFTDIYGRVPD
KAMKS

Sequences:

>Translated_485_residues
MSTLIPCIIAGGSGTRLWPVSREAMPKPFMRLPDGQSLLQKTFLRAVGLPGVERLLTVTNREVYFRTVDDYRQLNKGNAR
LDFILEPFGRNTAAAVAAAALHVSQLYGDEAQLLVLPADHLITDVAEFSRAVTAAQKLAAEGWLVTFGILPTAPETGFGY
IEKGPVLNADACQVARFVEKPDAITAQGYLDSGQHFWNAGMFCMRADAVLRELRKHAPDVLSAVSACLESSPRKEGNREL
QVELDSESFALTPDISIDYALMERSEKVAVVPCQLGWSDIGSWQAVRELSPVDAQGNYCNGETVLHDVSNCYIDSPKRLV
GAIGLDNLIIIDTPDALLIADGGRSQDVRIIAQELKRQGHDAYRLHRTVTRPWGTYTVLEEGKRFKIKRIVVRPQASLSL
QMHHHRSEHWIVVSGMARVTNGEREFMLDTNESTFIKPGHTHRLVNPGVIDLVMIEVQSGEYLGEDDIVRFTDIYGRVPD
KAMKS
>Mature_484_residues
STLIPCIIAGGSGTRLWPVSREAMPKPFMRLPDGQSLLQKTFLRAVGLPGVERLLTVTNREVYFRTVDDYRQLNKGNARL
DFILEPFGRNTAAAVAAAALHVSQLYGDEAQLLVLPADHLITDVAEFSRAVTAAQKLAAEGWLVTFGILPTAPETGFGYI
EKGPVLNADACQVARFVEKPDAITAQGYLDSGQHFWNAGMFCMRADAVLRELRKHAPDVLSAVSACLESSPRKEGNRELQ
VELDSESFALTPDISIDYALMERSEKVAVVPCQLGWSDIGSWQAVRELSPVDAQGNYCNGETVLHDVSNCYIDSPKRLVG
AIGLDNLIIIDTPDALLIADGGRSQDVRIIAQELKRQGHDAYRLHRTVTRPWGTYTVLEEGKRFKIKRIVVRPQASLSLQ
MHHHRSEHWIVVSGMARVTNGEREFMLDTNESTFIKPGHTHRLVNPGVIDLVMIEVQSGEYLGEDDIVRFTDIYGRVPDK
AMKS

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O9 antigen [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=483, Percent_Identity=45.1345755693582, Blast_Score=399, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 53731; Mature: 53600

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLIPCIIAGGSGTRLWPVSREAMPKPFMRLPDGQSLLQKTFLRAVGLPGVERLLTVTN
CCCEEEEEEECCCCCEEEECCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCC
REVYFRTVDDYRQLNKGNARLDFILEPFGRNTAAAVAAAALHVSQLYGDEAQLLVLPADH
CEEEEEEHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHH
LITDVAEFSRAVTAAQKLAAEGWLVTFGILPTAPETGFGYIEKGPVLNADACQVARFVEK
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEECCCCCCCCHHHHHHHHHCC
PDAITAQGYLDSGQHFWNAGMFCMRADAVLRELRKHAPDVLSAVSACLESSPRKEGNREL
CCCEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEE
QVELDSESFALTPDISIDYALMERSEKVAVVPCQLGWSDIGSWQAVRELSPVDAQGNYCN
EEEECCCCEEECCCCCEEHHHHCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCC
GETVLHDVSNCYIDSPKRLVGAIGLDNLIIIDTPDALLIADGGRSQDVRIIAQELKRQGH
CCHHHHHHHHCEECCHHHHHHHCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCC
DAYRLHRTVTRPWGTYTVLEEGKRFKIKRIVVRPQASLSLQMHHHRSEHWIVVSGMARVT
HHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCEEEEEECCCCCCEEEEECCHHHC
NGEREFMLDTNESTFIKPGHTHRLVNPGVIDLVMIEVQSGEYLGEDDIVRFTDIYGRVPD
CCCEEEEEECCCCEEECCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEHHHCCCCH
KAMKS
HHHCC
>Mature Secondary Structure 
STLIPCIIAGGSGTRLWPVSREAMPKPFMRLPDGQSLLQKTFLRAVGLPGVERLLTVTN
CCEEEEEEECCCCCEEEECCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCC
REVYFRTVDDYRQLNKGNARLDFILEPFGRNTAAAVAAAALHVSQLYGDEAQLLVLPADH
CEEEEEEHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHH
LITDVAEFSRAVTAAQKLAAEGWLVTFGILPTAPETGFGYIEKGPVLNADACQVARFVEK
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEECCCCCCCCHHHHHHHHHCC
PDAITAQGYLDSGQHFWNAGMFCMRADAVLRELRKHAPDVLSAVSACLESSPRKEGNREL
CCCEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEE
QVELDSESFALTPDISIDYALMERSEKVAVVPCQLGWSDIGSWQAVRELSPVDAQGNYCN
EEEECCCCEEECCCCCEEHHHHCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCC
GETVLHDVSNCYIDSPKRLVGAIGLDNLIIIDTPDALLIADGGRSQDVRIIAQELKRQGH
CCHHHHHHHHCEECCHHHHHHHCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCC
DAYRLHRTVTRPWGTYTVLEEGKRFKIKRIVVRPQASLSLQMHHHRSEHWIVVSGMARVT
HHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCEEEEEECCCCCCEEEEECCHHHC
NGEREFMLDTNESTFIKPGHTHRLVNPGVIDLVMIEVQSGEYLGEDDIVRFTDIYGRVPD
CCCEEEEEECCCCEEECCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEHHHCCCCH
KAMKS
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7515042; 9004408 [H]