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The map label for this gene is topA [H]

Identifier: 66046516

GI number: 66046516

Start: 3932531

End: 3935143

Strand: Reverse

Name: topA [H]

Synonym: Psyr_3287

Alternate gene names: 66046516

Gene position: 3935143-3932531 (Counterclockwise)

Preceding gene: 66046517

Following gene: 66046515

Centisome position: 64.58

GC content: 60.31

Gene sequence:

>2613_bases
ATGGGCAAATCGCTGGTCATCGTGGAATCCCCGGCTAAGGCCAAGACCATCAACAAGTATTTGGGCAACCAGTACGTGGT
GAAGTCGAGTATCGGCCATATCCGAGACCTGCCCACCAGCGGTTCGGCGAGTGCCGCCAAAGATCCTGCCGCCAAGCGTG
GCAAGGCTGCTGCGGGCGAAGGCCCGGTACTGACGCCGAAAGAAAAGGCGCAACGCCAGCTGGTCTCGCGCATGGGAGTC
GATCCGGAACACGGCTGGAAAGCCAAGTACGAGATCCTCCCCGGCAAGGAAAAGGTCATCGAAGAGCTGCGCAAGCTCGC
CAAGGATGCCGACATCATCTATCTCGCGACGGACTTGGACCGCGAAGGGGAAGCCATTGCCTGGCACCTGCGCGAAGCCA
TCGGGGGGGATGACAGCCGCTACAAGCGCGTGGTGTTCAACGAGATCACCAAGAAAGCCATTCAGGAAGCGTTTTCCAAG
CCAGGCGAACTGGATATTGCCCGGGTCAACGCCCAGCAGGCGCGTCGCTTCCTCGACCGCGTCGTCGGCTACATGGTCTC
GCCGCTGCTCTGGCAGAAAGTCGCCCGTGGCTTGTCCGCCGGGCGTGTGCAGTCGGTCGCAGTAAAACTGGTGGTCGAGC
GCGAACGTGAAATCCGTGCGTTCAACCCAGAAGAGTATTGGGAGATCCACGCCGATCTGGGCACCGCCAAGGGCGCCAAT
GTGCGTTTCGAAGTGGCGCGTGAAAACGGCGAAGCATTCAAGCCGCTGAATGAAACCCAGGCCATGGCCGCCCTTGAAAA
GCTCAAGGCGTCCAGCTACAGCATCGTCAAGCGTGAAGACAAGCCGACCAGCAGCAAGCCATCGGCGCCGTTCATTACCT
CGACCCTGCAGCAGGCAGCGAGCAACCGCCTGGGCTTCGGCGTGAAGAAGACCATGATGATGGCTCAGCGCTTGTACGAA
GCGGGCTACATCACCTACATGCGTACCGACTCGACCAACCTGTCGGCTGACGCAGTGAGCATGGCGCGTGACTACATCGG
AACCGAATTCGGCAAAAAGTACCTGCCGGAGTCGCCCAACGTCTACAGCAGCAAGGAAGGCGCGCAAGAGGCGCACGAAG
CGATTCGTCCTTCCGACGTCAACACTCACCCGAGCAAACTGACCGGGATGGAGCGTGACGCCGAGCGTCTTTACGAGTTG
ATCTGGCGCCAGTTCGTGGCCTGCCAGATGCTGCCTGCGCAATACCTGTCGACCACCGTCACGGTCGCCGCGAGCGATTT
CGAGCTGCGCGCCAAGGGCCGCATCCTCAAGTTCGACGGTTACACCCGTGCACTGCCGCAGATGGCCAAGCCGGGCGATG
ACGATGTTCTGCCGGACATGGCGCAGGGCGAAACGCTGAAACTGAACAAGCTCGACCCAAGCCAGCACTTCACCAAGCCG
CCTGCGCGTTACTCGGAAGCGAGCCTGGTCAAGGAGATGGAAAAGCGTGGCATCGGCCGCCCGTCGACCTACGCTGCGAT
CATCTCGACCATTCAGGACCGTGGCTACGTGGCGTTGCATAACCGCCGCTTCTACTCGGAAAAGATGGGCGACATCGTCA
CTGGGCGACTGTCGGAAAGCTTTTCCAACCTGATGGACTATGGCTTCACCGCCGGGATGGAAGAGAACCTCGACGACGTG
GCTCAGGGTGAGCGTGACTGGAAGAACGTGCTGGACGAGTTCTACGGCGATTTCCGTAAAAAACTCGAGGTGGCCGAGGC
CGCTGATGGCGGCATGCGCGCCAACCAGCCGGTGATGACGGATATCCCGTGCAAGGTGTGCGGCCGTCCAATGCAGATCC
GTACTGCCTCGACGGGCGTTTTCCTGGGTTGCTCCGGCTACAGCCTGCCACCCAAGGAACGCTGCAAGGCGACCGTCAAT
CTGACCCCCGGCGACGAAATTGCCGACGATGATGAGGGTGAGTCCGAGTCGCGGGTTCTGCGCGGCAAGCATCGCTGCCC
GATCTGCAGCACGGCGATGGACGCCTACCTGCTGGACGAGAAGCACAAGCTGCATATCTGCGGCAACAACCCCGATTGCA
CCGGTTACGAGATCGAGGAAGGCACTTATCGCATCAAAGGCTATGAAGGTCCGAGCCTGGAATGTGACAAGTGCGGCAGC
GAGATGCAGCTCAAGACCGGCCGTTTCGGCAAGTTCTTCGGCTGCACCAATCCGGACTGCAAGAACACCCGTAAACTGCT
GAAAAGCGGCGACGCGGCGCCGCCGAAGATGGACCCGGTGAAAATGCCGGAGCTCAAGTGCGACAAGGTTGACGATACTT
ACATACTGCGTGACGGGGCTTCGGGTCTGTTCCTGGCCGCCAGCCAGTTCCCGAAGAATCGCGAAACCCGCGCGCCGCTG
GTCATCGAAATCGCCCCGCACAAGGACGAAATCGATCCCAAGTATCACTTCCTTTGCGAAGCGCCGAAGAAGGATCCGGA
CGGTCGTCCAGCCGTGATCCGCTACAGTCGCAAGACCAAAGAGCAATACGTCCAGACCGAAGTAGAAGGCAAGCCTACCG
GCTGGCGTGCGTTCTACGACGGCAGCGCCTGGAAGGTTGAAGACAAGCGTTGA

Upstream 100 bases:

>100_bases
AACAGGCTGTGCTTGACCTCACCTTGTAGCCACTATATATAGCGTGGCCTTTACAAGCTCCTCACTGTCTTTCATCTAGT
ATTCAGGAATTTTCTGATCC

Downstream 100 bases:

>100_bases
TCGCAAATGTCCGGCTCAAGGGAGTCGGGCGCTGTTTTCGACAGGCATCCGCCTGTTTCGCCGGACTGTCCAGGCGTCAC
AGGCTATGCTGTTTTCTATC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 870; Mature: 869

Protein sequence:

>870_residues
MGKSLVIVESPAKAKTINKYLGNQYVVKSSIGHIRDLPTSGSASAAKDPAAKRGKAAAGEGPVLTPKEKAQRQLVSRMGV
DPEHGWKAKYEILPGKEKVIEELRKLAKDADIIYLATDLDREGEAIAWHLREAIGGDDSRYKRVVFNEITKKAIQEAFSK
PGELDIARVNAQQARRFLDRVVGYMVSPLLWQKVARGLSAGRVQSVAVKLVVEREREIRAFNPEEYWEIHADLGTAKGAN
VRFEVARENGEAFKPLNETQAMAALEKLKASSYSIVKREDKPTSSKPSAPFITSTLQQAASNRLGFGVKKTMMMAQRLYE
AGYITYMRTDSTNLSADAVSMARDYIGTEFGKKYLPESPNVYSSKEGAQEAHEAIRPSDVNTHPSKLTGMERDAERLYEL
IWRQFVACQMLPAQYLSTTVTVAASDFELRAKGRILKFDGYTRALPQMAKPGDDDVLPDMAQGETLKLNKLDPSQHFTKP
PARYSEASLVKEMEKRGIGRPSTYAAIISTIQDRGYVALHNRRFYSEKMGDIVTGRLSESFSNLMDYGFTAGMEENLDDV
AQGERDWKNVLDEFYGDFRKKLEVAEAADGGMRANQPVMTDIPCKVCGRPMQIRTASTGVFLGCSGYSLPPKERCKATVN
LTPGDEIADDDEGESESRVLRGKHRCPICSTAMDAYLLDEKHKLHICGNNPDCTGYEIEEGTYRIKGYEGPSLECDKCGS
EMQLKTGRFGKFFGCTNPDCKNTRKLLKSGDAAPPKMDPVKMPELKCDKVDDTYILRDGASGLFLAASQFPKNRETRAPL
VIEIAPHKDEIDPKYHFLCEAPKKDPDGRPAVIRYSRKTKEQYVQTEVEGKPTGWRAFYDGSAWKVEDKR

Sequences:

>Translated_870_residues
MGKSLVIVESPAKAKTINKYLGNQYVVKSSIGHIRDLPTSGSASAAKDPAAKRGKAAAGEGPVLTPKEKAQRQLVSRMGV
DPEHGWKAKYEILPGKEKVIEELRKLAKDADIIYLATDLDREGEAIAWHLREAIGGDDSRYKRVVFNEITKKAIQEAFSK
PGELDIARVNAQQARRFLDRVVGYMVSPLLWQKVARGLSAGRVQSVAVKLVVEREREIRAFNPEEYWEIHADLGTAKGAN
VRFEVARENGEAFKPLNETQAMAALEKLKASSYSIVKREDKPTSSKPSAPFITSTLQQAASNRLGFGVKKTMMMAQRLYE
AGYITYMRTDSTNLSADAVSMARDYIGTEFGKKYLPESPNVYSSKEGAQEAHEAIRPSDVNTHPSKLTGMERDAERLYEL
IWRQFVACQMLPAQYLSTTVTVAASDFELRAKGRILKFDGYTRALPQMAKPGDDDVLPDMAQGETLKLNKLDPSQHFTKP
PARYSEASLVKEMEKRGIGRPSTYAAIISTIQDRGYVALHNRRFYSEKMGDIVTGRLSESFSNLMDYGFTAGMEENLDDV
AQGERDWKNVLDEFYGDFRKKLEVAEAADGGMRANQPVMTDIPCKVCGRPMQIRTASTGVFLGCSGYSLPPKERCKATVN
LTPGDEIADDDEGESESRVLRGKHRCPICSTAMDAYLLDEKHKLHICGNNPDCTGYEIEEGTYRIKGYEGPSLECDKCGS
EMQLKTGRFGKFFGCTNPDCKNTRKLLKSGDAAPPKMDPVKMPELKCDKVDDTYILRDGASGLFLAASQFPKNRETRAPL
VIEIAPHKDEIDPKYHFLCEAPKKDPDGRPAVIRYSRKTKEQYVQTEVEGKPTGWRAFYDGSAWKVEDKR
>Mature_869_residues
GKSLVIVESPAKAKTINKYLGNQYVVKSSIGHIRDLPTSGSASAAKDPAAKRGKAAAGEGPVLTPKEKAQRQLVSRMGVD
PEHGWKAKYEILPGKEKVIEELRKLAKDADIIYLATDLDREGEAIAWHLREAIGGDDSRYKRVVFNEITKKAIQEAFSKP
GELDIARVNAQQARRFLDRVVGYMVSPLLWQKVARGLSAGRVQSVAVKLVVEREREIRAFNPEEYWEIHADLGTAKGANV
RFEVARENGEAFKPLNETQAMAALEKLKASSYSIVKREDKPTSSKPSAPFITSTLQQAASNRLGFGVKKTMMMAQRLYEA
GYITYMRTDSTNLSADAVSMARDYIGTEFGKKYLPESPNVYSSKEGAQEAHEAIRPSDVNTHPSKLTGMERDAERLYELI
WRQFVACQMLPAQYLSTTVTVAASDFELRAKGRILKFDGYTRALPQMAKPGDDDVLPDMAQGETLKLNKLDPSQHFTKPP
ARYSEASLVKEMEKRGIGRPSTYAAIISTIQDRGYVALHNRRFYSEKMGDIVTGRLSESFSNLMDYGFTAGMEENLDDVA
QGERDWKNVLDEFYGDFRKKLEVAEAADGGMRANQPVMTDIPCKVCGRPMQIRTASTGVFLGCSGYSLPPKERCKATVNL
TPGDEIADDDEGESESRVLRGKHRCPICSTAMDAYLLDEKHKLHICGNNPDCTGYEIEEGTYRIKGYEGPSLECDKCGSE
MQLKTGRFGKFFGCTNPDCKNTRKLLKSGDAAPPKMDPVKMPELKCDKVDDTYILRDGASGLFLAASQFPKNRETRAPLV
IEIAPHKDEIDPKYHFLCEAPKKDPDGRPAVIRYSRKTKEQYVQTEVEGKPTGWRAFYDGSAWKVEDKR

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=527, Percent_Identity=27.134724857685, Blast_Score=152, Evalue=2e-36,
Organism=Homo sapiens, GI10835218, Length=609, Percent_Identity=26.7651888341544, Blast_Score=145, Evalue=2e-34,
Organism=Escherichia coli, GI1787529, Length=873, Percent_Identity=66.0939289805269, Blast_Score=1211, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=549, Percent_Identity=22.9508196721311, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32563869, Length=537, Percent_Identity=27.1880819366853, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17555378, Length=529, Percent_Identity=26.4650283553875, Blast_Score=128, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=487, Percent_Identity=24.2299794661191, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24585251, Length=593, Percent_Identity=25.2951096121417, Blast_Score=135, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640096, Length=511, Percent_Identity=25.440313111546, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 96969; Mature: 96838

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSLVIVESPAKAKTINKYLGNQYVVKSSIGHIRDLPTSGSASAAKDPAAKRGKAAAGE
CCCCEEEEECCCHHHHHHHHHCCCEEEHHHCCHHHCCCCCCCCCCCCCCHHHCCCCCCCC
GPVLTPKEKAQRQLVSRMGVDPEHGWKAKYEILPGKEKVIEELRKLAKDADIIYLATDLD
CCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCC
REGEAIAWHLREAIGGDDSRYKRVVFNEITKKAIQEAFSKPGELDIARVNAQQARRFLDR
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHH
VVGYMVSPLLWQKVARGLSAGRVQSVAVKLVVEREREIRAFNPEEYWEIHADLGTAKGAN
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCC
VRFEVARENGEAFKPLNETQAMAALEKLKASSYSIVKREDKPTSSKPSAPFITSTLQQAA
EEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
SNRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSADAVSMARDYIGTEFGKKYLPESPN
HCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VYSSKEGAQEAHEAIRPSDVNTHPSKLTGMERDAERLYELIWRQFVACQMLPAQYLSTTV
CCCCCHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
TVAASDFELRAKGRILKFDGYTRALPQMAKPGDDDVLPDMAQGETLKLNKLDPSQHFTKP
EEEECCEEEEECCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHCCCC
PARYSEASLVKEMEKRGIGRPSTYAAIISTIQDRGYVALHNRRFYSEKMGDIVTGRLSES
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHH
FSNLMDYGFTAGMEENLDDVAQGERDWKNVLDEFYGDFRKKLEVAEAADGGMRANQPVMT
HHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
DIPCKVCGRPMQIRTASTGVFLGCSGYSLPPKERCKATVNLTPGDEIADDDEGESESRVL
CCCHHHCCCCEEEEEECCCEEEECCCCCCCCHHHCCEEEECCCCCCCCCCCCCCCHHHHH
RGKHRCPICSTAMDAYLLDEKHKLHICGNNPDCTGYEIEEGTYRIKGYEGPSLECDKCGS
HCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCEEEEECCCCCCCCHHHCCC
EMQLKTGRFGKFFGCTNPDCKNTRKLLKSGDAAPPKMDPVKMPELKCDKVDDTYILRDGA
CCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
SGLFLAASQFPKNRETRAPLVIEIAPHKDEIDPKYHFLCEAPKKDPDGRPAVIRYSRKTK
CCEEEEHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCEEEEECHHHH
EQYVQTEVEGKPTGWRAFYDGSAWKVEDKR
HHHHHHHCCCCCCCEEEEECCCEEEECCCC
>Mature Secondary Structure 
GKSLVIVESPAKAKTINKYLGNQYVVKSSIGHIRDLPTSGSASAAKDPAAKRGKAAAGE
CCCEEEEECCCHHHHHHHHHCCCEEEHHHCCHHHCCCCCCCCCCCCCCHHHCCCCCCCC
GPVLTPKEKAQRQLVSRMGVDPEHGWKAKYEILPGKEKVIEELRKLAKDADIIYLATDLD
CCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCC
REGEAIAWHLREAIGGDDSRYKRVVFNEITKKAIQEAFSKPGELDIARVNAQQARRFLDR
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHH
VVGYMVSPLLWQKVARGLSAGRVQSVAVKLVVEREREIRAFNPEEYWEIHADLGTAKGAN
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCC
VRFEVARENGEAFKPLNETQAMAALEKLKASSYSIVKREDKPTSSKPSAPFITSTLQQAA
EEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
SNRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSADAVSMARDYIGTEFGKKYLPESPN
HCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VYSSKEGAQEAHEAIRPSDVNTHPSKLTGMERDAERLYELIWRQFVACQMLPAQYLSTTV
CCCCCHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
TVAASDFELRAKGRILKFDGYTRALPQMAKPGDDDVLPDMAQGETLKLNKLDPSQHFTKP
EEEECCEEEEECCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHCCCC
PARYSEASLVKEMEKRGIGRPSTYAAIISTIQDRGYVALHNRRFYSEKMGDIVTGRLSES
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHH
FSNLMDYGFTAGMEENLDDVAQGERDWKNVLDEFYGDFRKKLEVAEAADGGMRANQPVMT
HHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
DIPCKVCGRPMQIRTASTGVFLGCSGYSLPPKERCKATVNLTPGDEIADDDEGESESRVL
CCCHHHCCCCEEEEEECCCEEEECCCCCCCCHHHCCEEEECCCCCCCCCCCCCCCHHHHH
RGKHRCPICSTAMDAYLLDEKHKLHICGNNPDCTGYEIEEGTYRIKGYEGPSLECDKCGS
HCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCEEEEECCCCCCCCHHHCCC
EMQLKTGRFGKFFGCTNPDCKNTRKLLKSGDAAPPKMDPVKMPELKCDKVDDTYILRDGA
CCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
SGLFLAASQFPKNRETRAPLVIEIAPHKDEIDPKYHFLCEAPKKDPDGRPAVIRYSRKTK
CCEEEEHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCEEEEECHHHH
EQYVQTEVEGKPTGWRAFYDGSAWKVEDKR
HHHHHHHCCCCCCCEEEEECCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]