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The map label for this gene is phaD [H]

Identifier: 66046483

GI number: 66046483

Start: 3899067

End: 3900752

Strand: Direct

Name: phaD [H]

Synonym: Psyr_3254

Alternate gene names: 66046483

Gene position: 3899067-3900752 (Clockwise)

Preceding gene: 66046482

Following gene: 66046484

Centisome position: 63.99

GC content: 62.1

Gene sequence:

>1686_bases
ATGAGCTGGATGAACCAACTGATCATCGCGCCCATCCTGCTGCCGCTGCTGACCGCCGGGCTGATGCTCTGGCTGGGCGA
AAAGCACCGGCCGCTCAAGGGCCGTATCAATCTGTTTTCCAGCATCCTCGGGCTGGGCATTTCCGTAGCGCTGTTTCTCT
GGGTGCAACACAGCGGCAGCAGCGGCTCGATCGGTGTCTATCTGCCGGGCAACTGGGGCGTGCCGTTCGGCATCGTGCTG
GTGGTGGACCACCTGTCAGCGATGATGCTGGTGCTGACCGGGATCGTCGGGGTCTGCGCCCTGCTGTTCGCGCTGGCTCG
CTGGGACAGGGCCGGTGCCAGCTTCCATGCGCTGTTCCAGATCCAGCTCATGGGGCTGTACGGTGCGTTCCTGACCGCTG
ACCTGTTCAACCTGTTCGTGTTCTTCGAAGTACTGCTCGCAGCGTCCTACGGCTTGATGTTGCATGGCTCTGGCCGGGCA
AGGGTGTCGACGGGGCTGCATTACATTTCGATCAACCTGCTGGCTTCGTCGTTGTTTCTGATCGGTGCTGCGTTGATTTA
CGGGGTGACCGGCACGCTGAACTTTGCCGATCTGGCCATCAAGATCCTGCAGGTGGCGGAGTCCGATCTGGGCCTGCTGC
ATGCCGGAGCTGCGATTCTGGCCACGGCGTTCCTGGCCAAGGCCGGCATGTGGCCGCTGAACTTCTGGCTGGTTCCCGCC
TACTCGGCAGCCAGCGCGCCGGTAGCGGCAATGTTTGCGATCATGACCAAAGTGGGTGTCTATACCCTGCTACGCCTGTG
GACCCTGCTGTTTTCCGGTCAGGCCGGTGCCTCGGCATTCTTCGGCGGCGACTGGCTGATCTACGGCGGCATGGCGACGA
TTTTCACCGCGGCCATCGCCATCATCGCCGCCCAGCGCCTGGAGCGCCTCGCCAGCCTGAGCATACTGGTGTCAGCGGGC
ATTCTGCTGTCCGCCGTCGGCTTCGCCCAGCCGAGCCTGACCTCGGGCGCGCTGTTCTATATGGTCAGCTCGACGCTGGC
ATTGAGCGCCATGTTTCTGCTGGGCGAACTGATCGAACGCTCCCGCTCGGCCAACGAGATCCCACTGGAATACGACGTAG
ATCAATTGCCTCGTACCCTGGAGTCCCTGCATCCGCCGCCCGGTGCCAACCTGGACGACGAACAGAAGGTCGTGGTCGGT
CAGGTCATTCCCATGACCATGGCTTTTCTGGGCCTGAGTTTTATCGCCTGCGCCTTGCTGATCATCGGCATGCCGCCGCT
CTCGGGATTCATCGGCAAGCTCACCTTGATCAGCGCGCTGATGAACCCGCTGGGGCTGGACGTCGCGAAGGATGAGGCGC
TTTCCACTCAGGCATGGATGCTGATCGGCCTGCTGATTTTTTCCGGGCTGGCGTCGCTGATCGCTTTTTCAAGGCTCGGC
ACGCAACGTTTCTGGACGCCACACGAGCGCCCTTCGCCGATGCTGCGTCGCAACGAATGCCTGCCGATCATCATTCTTCT
GGGTCTGTGCATCGCGCTGACCTTCAAGGCCGAACCCTTGTTGCGCTACACCCAGGACACCGCCGCAGCGCTGCATGAGC
CCAAACAGTATGTGCAGTCAGTGCTCGCCACACGCCCGATACCCGGCCCCACCAGCCAGCAAGCAGATCCGGAGGAGCAG
CCATGA

Upstream 100 bases:

>100_bases
TCGTCATCAGCTTCGCCATGACCGCGCTGTTTCTGGTGGTCTTGCTCGCCTCCAGAGGCCTGACCGGCACCGACCATGTA
GATGGCCGGGAGCCCAAGGC

Downstream 100 bases:

>100_bases
AGCGCCTGTTTCCCGCCCCGCTGCTCTCGTTGGCACTCTGGCTGTTGTGGTTGGTATTGAATCTGTCGATCAGCCCCGGT
CACCTGCTGCTGGGCGCGGT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MSWMNQLIIAPILLPLLTAGLMLWLGEKHRPLKGRINLFSSILGLGISVALFLWVQHSGSSGSIGVYLPGNWGVPFGIVL
VVDHLSAMMLVLTGIVGVCALLFALARWDRAGASFHALFQIQLMGLYGAFLTADLFNLFVFFEVLLAASYGLMLHGSGRA
RVSTGLHYISINLLASSLFLIGAALIYGVTGTLNFADLAIKILQVAESDLGLLHAGAAILATAFLAKAGMWPLNFWLVPA
YSAASAPVAAMFAIMTKVGVYTLLRLWTLLFSGQAGASAFFGGDWLIYGGMATIFTAAIAIIAAQRLERLASLSILVSAG
ILLSAVGFAQPSLTSGALFYMVSSTLALSAMFLLGELIERSRSANEIPLEYDVDQLPRTLESLHPPPGANLDDEQKVVVG
QVIPMTMAFLGLSFIACALLIIGMPPLSGFIGKLTLISALMNPLGLDVAKDEALSTQAWMLIGLLIFSGLASLIAFSRLG
TQRFWTPHERPSPMLRRNECLPIIILLGLCIALTFKAEPLLRYTQDTAAALHEPKQYVQSVLATRPIPGPTSQQADPEEQ
P

Sequences:

>Translated_561_residues
MSWMNQLIIAPILLPLLTAGLMLWLGEKHRPLKGRINLFSSILGLGISVALFLWVQHSGSSGSIGVYLPGNWGVPFGIVL
VVDHLSAMMLVLTGIVGVCALLFALARWDRAGASFHALFQIQLMGLYGAFLTADLFNLFVFFEVLLAASYGLMLHGSGRA
RVSTGLHYISINLLASSLFLIGAALIYGVTGTLNFADLAIKILQVAESDLGLLHAGAAILATAFLAKAGMWPLNFWLVPA
YSAASAPVAAMFAIMTKVGVYTLLRLWTLLFSGQAGASAFFGGDWLIYGGMATIFTAAIAIIAAQRLERLASLSILVSAG
ILLSAVGFAQPSLTSGALFYMVSSTLALSAMFLLGELIERSRSANEIPLEYDVDQLPRTLESLHPPPGANLDDEQKVVVG
QVIPMTMAFLGLSFIACALLIIGMPPLSGFIGKLTLISALMNPLGLDVAKDEALSTQAWMLIGLLIFSGLASLIAFSRLG
TQRFWTPHERPSPMLRRNECLPIIILLGLCIALTFKAEPLLRYTQDTAAALHEPKQYVQSVLATRPIPGPTSQQADPEEQ
P
>Mature_560_residues
SWMNQLIIAPILLPLLTAGLMLWLGEKHRPLKGRINLFSSILGLGISVALFLWVQHSGSSGSIGVYLPGNWGVPFGIVLV
VDHLSAMMLVLTGIVGVCALLFALARWDRAGASFHALFQIQLMGLYGAFLTADLFNLFVFFEVLLAASYGLMLHGSGRAR
VSTGLHYISINLLASSLFLIGAALIYGVTGTLNFADLAIKILQVAESDLGLLHAGAAILATAFLAKAGMWPLNFWLVPAY
SAASAPVAAMFAIMTKVGVYTLLRLWTLLFSGQAGASAFFGGDWLIYGGMATIFTAAIAIIAAQRLERLASLSILVSAGI
LLSAVGFAQPSLTSGALFYMVSSTLALSAMFLLGELIERSRSANEIPLEYDVDQLPRTLESLHPPPGANLDDEQKVVVGQ
VIPMTMAFLGLSFIACALLIIGMPPLSGFIGKLTLISALMNPLGLDVAKDEALSTQAWMLIGLLIFSGLASLIAFSRLGT
QRFWTPHERPSPMLRRNECLPIIILLGLCIALTFKAEPLLRYTQDTAAALHEPKQYVQSVLATRPIPGPTSQQADPEEQP

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=379, Percent_Identity=29.8153034300792, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788827, Length=517, Percent_Identity=25.7253384912959, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788831, Length=425, Percent_Identity=24, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 60067; Mature: 59936

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWMNQLIIAPILLPLLTAGLMLWLGEKHRPLKGRINLFSSILGLGISVALFLWVQHSGS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCC
SGSIGVYLPGNWGVPFGIVLVVDHLSAMMLVLTGIVGVCALLFALARWDRAGASFHALFQ
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IQLMGLYGAFLTADLFNLFVFFEVLLAASYGLMLHGSGRARVSTGLHYISINLLASSLFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHCHHHHHHHHHHHHHHH
IGAALIYGVTGTLNFADLAIKILQVAESDLGLLHAGAAILATAFLAKAGMWPLNFWLVPA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
YSAASAPVAAMFAIMTKVGVYTLLRLWTLLFSGQAGASAFFGGDWLIYGGMATIFTAAIA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEEECCHHHHHHHHHH
IIAAQRLERLASLSILVSAGILLSAVGFAQPSLTSGALFYMVSSTLALSAMFLLGELIER
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SRSANEIPLEYDVDQLPRTLESLHPPPGANLDDEQKVVVGQVIPMTMAFLGLSFIACALL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IIGMPPLSGFIGKLTLISALMNPLGLDVAKDEALSTQAWMLIGLLIFSGLASLIAFSRLG
HHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TQRFWTPHERPSPMLRRNECLPIIILLGLCIALTFKAEPLLRYTQDTAAALHEPKQYVQS
CHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VLATRPIPGPTSQQADPEEQP
HHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SWMNQLIIAPILLPLLTAGLMLWLGEKHRPLKGRINLFSSILGLGISVALFLWVQHSGS
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCC
SGSIGVYLPGNWGVPFGIVLVVDHLSAMMLVLTGIVGVCALLFALARWDRAGASFHALFQ
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IQLMGLYGAFLTADLFNLFVFFEVLLAASYGLMLHGSGRARVSTGLHYISINLLASSLFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHCHHHHHHHHHHHHHHH
IGAALIYGVTGTLNFADLAIKILQVAESDLGLLHAGAAILATAFLAKAGMWPLNFWLVPA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
YSAASAPVAAMFAIMTKVGVYTLLRLWTLLFSGQAGASAFFGGDWLIYGGMATIFTAAIA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEEECCHHHHHHHHHH
IIAAQRLERLASLSILVSAGILLSAVGFAQPSLTSGALFYMVSSTLALSAMFLLGELIER
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SRSANEIPLEYDVDQLPRTLESLHPPPGANLDDEQKVVVGQVIPMTMAFLGLSFIACALL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IIGMPPLSGFIGKLTLISALMNPLGLDVAKDEALSTQAWMLIGLLIFSGLASLIAFSRLG
HHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TQRFWTPHERPSPMLRRNECLPIIILLGLCIALTFKAEPLLRYTQDTAAALHEPKQYVQS
CHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VLATRPIPGPTSQQADPEEQP
HHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]