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The map label for this gene is flbD [H]

Identifier: 66046477

GI number: 66046477

Start: 3891187

End: 3892293

Strand: Direct

Name: flbD [H]

Synonym: Psyr_3248

Alternate gene names: 66046477

Gene position: 3891187-3892293 (Clockwise)

Preceding gene: 66046476

Following gene: 66046480

Centisome position: 63.86

GC content: 61.43

Gene sequence:

>1107_bases
ATGTCCGGGCAACTGCTGACCTTACCCCACTCGCCTGCGCTGGCCACCTCGATCAGGGCCACTGCGCAGGTGTTCGAAGA
CCCCAAGTCGCGAGCCCTGCTCGCTCACGTACGCCAGGTTGCGCCCAGCGATGCCAGTGTGCTGATCATCGGCGAGACCG
GCACCGGCAAGGAACTGGTTGCTCGCCACGTCCACGAACTGAGCGCCCGCCGCGACAAACCGTTCGTGGCGGTCAACTGC
GGCGCGTTCTCCGAGAACCTGATCGAGGCGGAGTTGTTCGGCCACGAAAAAGGCGCGTTCACCGGAGCGATCAGCGCCAA
GGCCGGCTGGTTTGAAGAGGCCAACGGCGGCACGCTGTTTCTCGATGAGATCGGCGACCTGCCCATGACCTTGCAGGTCA
AATTGCTGCGCGTGCTTCAGGAGCGTGAAGTGGTGCGTCTGGGCTCGCGAAAGAGTGTGCCCATTGATGTAAGGGTGCTC
GCAGCCACCAACGTGCAACTGGAAAAGGCCATCAACGCCGGGCATTTCCGCGAGGACCTTTACTACCGGCTCGACGTGGT
TAATCTGGAATTGAGCCCGCTGCGCGAGCGGCCGGGCGATATCCTGCCCCTGGCCCGGCACTTCATAGAGGTCTACAGCC
AGCGGCTGCGGCATGGTCCGGTGCGGATCAGCCCCGAAGCCGAGCACAAGCTCAAGACCTACAGCTGGCCAGGCAATATT
CGCGAGCTGGAAAACGTCATCCATCACACCCTGCTGGTGTGCCGTGAAGGTGTCATTCAGCGTGATGACCTGCGCATGTC
GAACCTTAGAATCGAGCGCCAGGATGAACACAACCCGCCTGACGAATCCGCCGAGCAACTGCTCAACCGGGCGTTTCAGA
AACTGTTTGAAGAACAGGCCGGTGCCTTGCACGAGAAGGTCGAAGATACGTTGCTCAGAACCGCCTATCGCTTCTGCCAC
CACAACCAGGTGCACACGGCCAGCCTGCTGGGCCTGACCCGCAACGTCACGCGCACACGTCTGATCAAGATTGGCGAACT
GGCGGTCAACAAACGCAGGCCGGGTGAAAATGTCCAGGGCGACCGCATGCTGCACCTGTCGGTGTGA

Upstream 100 bases:

>100_bases
TGCCTGAGCCTTACGCCAGCCTCGCCGGGCGCGGCATCACCAACCTGACGGGCCCGTTCGGCGAAATGATCGCCAACGAT
CTGCCCCCGCAGGCCAAGTG

Downstream 100 bases:

>100_bases
AGGCGCAGACCGATGACGCGGAGCGTCACGAACGGCATTCCCACGCTGGAGCGTGAGGAACGATAGCTGTCCGGAAGAAC
ACCATCGTGCCCATGCTCCG

Product: helix-turn-helix, Fis-type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSGQLLTLPHSPALATSIRATAQVFEDPKSRALLAHVRQVAPSDASVLIIGETGTGKELVARHVHELSARRDKPFVAVNC
GAFSENLIEAELFGHEKGAFTGAISAKAGWFEEANGGTLFLDEIGDLPMTLQVKLLRVLQEREVVRLGSRKSVPIDVRVL
AATNVQLEKAINAGHFREDLYYRLDVVNLELSPLRERPGDILPLARHFIEVYSQRLRHGPVRISPEAEHKLKTYSWPGNI
RELENVIHHTLLVCREGVIQRDDLRMSNLRIERQDEHNPPDESAEQLLNRAFQKLFEEQAGALHEKVEDTLLRTAYRFCH
HNQVHTASLLGLTRNVTRTRLIKIGELAVNKRRPGENVQGDRMLHLSV

Sequences:

>Translated_368_residues
MSGQLLTLPHSPALATSIRATAQVFEDPKSRALLAHVRQVAPSDASVLIIGETGTGKELVARHVHELSARRDKPFVAVNC
GAFSENLIEAELFGHEKGAFTGAISAKAGWFEEANGGTLFLDEIGDLPMTLQVKLLRVLQEREVVRLGSRKSVPIDVRVL
AATNVQLEKAINAGHFREDLYYRLDVVNLELSPLRERPGDILPLARHFIEVYSQRLRHGPVRISPEAEHKLKTYSWPGNI
RELENVIHHTLLVCREGVIQRDDLRMSNLRIERQDEHNPPDESAEQLLNRAFQKLFEEQAGALHEKVEDTLLRTAYRFCH
HNQVHTASLLGLTRNVTRTRLIKIGELAVNKRRPGENVQGDRMLHLSV
>Mature_367_residues
SGQLLTLPHSPALATSIRATAQVFEDPKSRALLAHVRQVAPSDASVLIIGETGTGKELVARHVHELSARRDKPFVAVNCG
AFSENLIEAELFGHEKGAFTGAISAKAGWFEEANGGTLFLDEIGDLPMTLQVKLLRVLQEREVVRLGSRKSVPIDVRVLA
ATNVQLEKAINAGHFREDLYYRLDVVNLELSPLRERPGDILPLARHFIEVYSQRLRHGPVRISPEAEHKLKTYSWPGNIR
ELENVIHHTLLVCREGVIQRDDLRMSNLRIERQDEHNPPDESAEQLLNRAFQKLFEEQAGALHEKVEDTLLRTAYRFCHH
NQVHTASLLGLTRNVTRTRLIKIGELAVNKRRPGENVQGDRMLHLSV

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=232, Percent_Identity=54.7413793103448, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI1790437, Length=243, Percent_Identity=51.440329218107, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1788550, Length=252, Percent_Identity=48.8095238095238, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1788905, Length=240, Percent_Identity=53.3333333333333, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1789233, Length=303, Percent_Identity=41.2541254125413, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1790299, Length=327, Percent_Identity=40.9785932721713, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1789087, Length=229, Percent_Identity=51.9650655021834, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1786524, Length=240, Percent_Identity=52.0833333333333, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI87082152, Length=250, Percent_Identity=49.2, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI87081872, Length=324, Percent_Identity=40.4320987654321, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1787583, Length=238, Percent_Identity=39.9159663865546, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87081858, Length=239, Percent_Identity=34.3096234309623, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789828, Length=246, Percent_Identity=34.9593495934959, Blast_Score=135, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 41409; Mature: 41278

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGQLLTLPHSPALATSIRATAQVFEDPKSRALLAHVRQVAPSDASVLIIGETGTGKELV
CCCCEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHH
ARHVHELSARRDKPFVAVNCGAFSENLIEAELFGHEKGAFTGAISAKAGWFEEANGGTLF
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEE
LDEIGDLPMTLQVKLLRVLQEREVVRLGSRKSVPIDVRVLAATNVQLEKAINAGHFREDL
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHCCCCHHHHH
YYRLDVVNLELSPLRERPGDILPLARHFIEVYSQRLRHGPVRISPEAEHKLKTYSWPGNI
EEEEEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHHCCEEECCCCCH
RELENVIHHTLLVCREGVIQRDDLRMSNLRIERQDEHNPPDESAEQLLNRAFQKLFEEQA
HHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
GALHEKVEDTLLRTAYRFCHHNQVHTASLLGLTRNVTRTRLIKIGELAVNKRRPGENVQG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DRMLHLSV
CEEEEEEC
>Mature Secondary Structure 
SGQLLTLPHSPALATSIRATAQVFEDPKSRALLAHVRQVAPSDASVLIIGETGTGKELV
CCCEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHH
ARHVHELSARRDKPFVAVNCGAFSENLIEAELFGHEKGAFTGAISAKAGWFEEANGGTLF
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEE
LDEIGDLPMTLQVKLLRVLQEREVVRLGSRKSVPIDVRVLAATNVQLEKAINAGHFREDL
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHCCCCHHHHH
YYRLDVVNLELSPLRERPGDILPLARHFIEVYSQRLRHGPVRISPEAEHKLKTYSWPGNI
EEEEEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHHCCEEECCCCCH
RELENVIHHTLLVCREGVIQRDDLRMSNLRIERQDEHNPPDESAEQLLNRAFQKLFEEQA
HHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
GALHEKVEDTLLRTAYRFCHHNQVHTASLLGLTRNVTRTRLIKIGELAVNKRRPGENVQG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DRMLHLSV
CEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]